ID DMA_BPT2 STANDARD; PRT; 259 AA. AC P12427; DT 01-OCT-1989 (Rel. 12, Created) DT 01-OCT-1989 (Rel. 12, Last sequence update) DT 10-MAY-2005 (Rel. 47, Last annotation update) DE DNA adenine methylase (EC 2.1.1.72) (Deoxyadenosyl-methyltransferase) DE (M.EcoT2Dam). GN Name=DAM; OS Bacteriophage T2. OC Viruses; dsDNA viruses, no RNA stage; Caudovirales; Myoviridae; OC T4-like viruses. OX NCBI_TaxID=10664; RN [1] RP NUCLEOTIDE SEQUENCE. RX MEDLINE=89033901; PubMed=3053648; RA Miner Z., Hattman S.; RT "Molecular cloning, sequencing, and mapping of the bacteriophage T2 RT dam gene."; RL J. Bacteriol. 170:5177-5184(1988). RN [2] RP MUTAGENESIS. RX MEDLINE=90045940; PubMed=2510127; RA Miner Z., Schlagman S.L., Hattman S.; RT "Single amino acid changes that alter the DNA sequence specificity of RT the DNA-[N6-adenine] methyltransferase (Dam) of bacteriophage T4."; RL Nucleic Acids Res. 17:8149-8157(1989). CC -!- FUNCTION: This methylase recognizes the double-stranded sequence CC GATC, causes specific methylation on A-2 on both strands. CC -!- CATALYTIC ACTIVITY: S-adenosyl-L-methionine + DNA adenine = S- CC adenosyl-L-homocysteine + DNA 6-methylaminopurine. CC -!- SIMILARITY: Belongs to the N4/N6-methyltransferase family. CC --------------------------------------------------------------------------- CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms CC Distributed under the Creative Commons Attribution (CC BY 4.0) License CC --------------------------------------------------------------------------- DR EMBL; M22342; AAA32477.1; -; Genomic_DNA. DR PIR; A30195; XYBPT2. DR SMR; P12427; 1-242. DR REBASE; 2685; M.EcoT2Dam. DR InterPro; IPR002294; D12N6_mtfrase. DR InterPro; IPR012326; Dam. DR InterPro; IPR012263; M_m6A_EcoRV. DR InterPro; IPR012327; MethyltransfD12. DR InterPro; IPR002052; N6_Mtase. DR Pfam; PF02086; MethyltransfD12; 1. DR PIRSF; PIRSF000398; M_m6A_EcoRV; 1. DR PRINTS; PR00505; D12N6MTFRASE. DR TIGRFAMs; TIGR00571; dam; 1. DR PROSITE; PS00092; N6_MTASE; 1. KW DNA replication; Methyltransferase; Transferase. SQ SEQUENCE 259 AA; 30439 MW; B3551303CFE71FD5 CRC64; MLGAIAYTGN KQSLLPELKP HFPKYDRFVD LFCGGLSVSL NVNGPVLAND IQEPIIEMYK RLINVSWDDV LKVIKQYKLS KTSKEEFLKL REDYNKTRDP LLLYVLHFHG FSNMIRINDK GNFTTPFGKR TINKNSEKRF NHFKQNCDKI IFSSLHFKDV KILDGDFVYV DPPYLITVAD YNKFWSEEEE KDLLNLLDSL NDRGIKFGLS NVLEHHGKEN TLLKEWSKKY NVKHLNKKYV FNIYHSKEKN GTDEVYIFN //