ID KAR1_YEAST Reviewed; 433 AA. AC P11927; D6W0Z9; E9P8Y4; DT 01-OCT-1989, integrated into UniProtKB/Swiss-Prot. DT 01-OCT-1996, sequence version 2. DT 31-MAY-2011, entry version 80. DE RecName: Full=Cell division control protein KAR1; GN Name=KAR1; OrderedLocusNames=YNL188W; ORFNames=N1611; OS Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast). OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; OC Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces. OX NCBI_TaxID=559292; RN [1] RP NUCLEOTIDE SEQUENCE [GENOMIC DNA]. RX MEDLINE=87159524; PubMed=3030557; DOI=10.1016/0092-8674(87)90712-4; RA Rose M.D., Fink G.R.; RT "KAR1, a gene required for function of both intranuclear and RT extranuclear microtubules in yeast."; RL Cell 48:1047-1060(1987). RN [2] RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. RC STRAIN=ATCC 204508 / S288c; RX MEDLINE=97313269; PubMed=9169873; RA Philippsen P., Kleine K., Poehlmann R., Duesterhoeft A., Hamberg K., RA Hegemann J.H., Obermaier B., Urrestarazu L.A., Aert R., Albermann K., RA Altmann R., Andre B., Baladron V., Ballesta J.P.G., Becam A.-M., RA Beinhauer J.D., Boskovic J., Buitrago M.J., Bussereau F., Coster F., RA Crouzet M., D'Angelo M., Dal Pero F., De Antoni A., del Rey F., RA Doignon F., Domdey H., Dubois E., Fiedler T.A., Fleig U., Floeth M., RA Fritz C., Gaillardin C., Garcia-Cantalejo J.M., Glansdorff N., RA Goffeau A., Gueldener U., Herbert C.J., Heumann K., Heuss-Neitzel D., RA Hilbert H., Hinni K., Iraqui Houssaini I., Jacquet M., Jimenez A., RA Jonniaux J.-L., Karpfinger-Hartl L., Lanfranchi G., Lepingle A., RA Levesque H., Lyck R., Maftahi M., Mallet L., Maurer C.T.C., RA Messenguy F., Mewes H.-W., Moestl D., Nasr F., Nicaud J.-M., RA Niedenthal R.K., Pandolfo D., Pierard A., Piravandi E., Planta R.J., RA Pohl T.M., Purnelle B., Rebischung C., Remacha M.A., Revuelta J.L., RA Rinke M., Saiz J.E., Sartorello F., Scherens B., Sen-Gupta M., RA Soler-Mira A., Urbanus J.H.M., Valle G., Van Dyck L., Verhasselt P., RA Vierendeels F., Vissers S., Voet M., Volckaert G., Wach A., RA Wambutt R., Wedler H., Zollner A., Hani J.; RT "The nucleotide sequence of Saccharomyces cerevisiae chromosome XIV RT and its evolutionary implications."; RL Nature 387:93-98(1997). RN [3] RP GENOME REANNOTATION. RC STRAIN=ATCC 204508 / S288c; RG Saccharomyces Genome Database; RL Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases. RN [4] RP NUCLEOTIDE SEQUENCE [GENOMIC DNA]. RC STRAIN=ATCC 204508 / S288c; RX PubMed=17322287; DOI=10.1101/gr.6037607; RA Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F., RA Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., RA Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., RA Williamson J., Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., RA Kolodner R.D., LaBaer J.; RT "Approaching a complete repository of sequence-verified protein- RT encoding clones for Saccharomyces cerevisiae."; RL Genome Res. 17:536-543(2007). RN [5] RP FUNCTION. RX MEDLINE=94350199; PubMed=8070654; RA Vallen E.A., Ho W., Winey M., Rose M.D.; RT "Genetic interactions between CDC31 and KAR1, two genes required for RT duplication of the microtubule organizing center in Saccharomyces RT cerevisiae."; RL Genetics 137:407-422(1994). RN [6] RP FUNCTION. RC STRAIN=ATCC 204508 / S288c; RX MEDLINE=95181541; PubMed=7876310; DOI=10.1083/jcb.128.5.863; RA Spang A., Courtney I., Grein K., Matzner M., Schiebel E.; RT "The Cdc31p-binding protein Kar1p is a component of the half bridge of RT the yeast spindle pole body."; RL J. Cell Biol. 128:863-877(1995). RN [7] RP FUNCTION, AND INTERACTION WITH SPC72. RX PubMed=10428957; DOI=10.1093/emboj/18.15.4180; RA Pereira G., Grueneberg U., Knop M., Schiebel E.; RT "Interaction of the yeast gamma-tubulin complex-binding protein Spc72p RT with Kar1p is essential for microtubule function during karyogamy."; RL EMBO J. 18:4180-4195(1999). CC -!- FUNCTION: KAR1 is required for function of both intranuclear and CC extranuclear microtubules. KAR1 helps localize CDC31 to the CC spindle pole body (SPB), CDC31 then initiates SPB duplication via CC interaction with a downstream effector. CC -!- SUBUNIT: Interacts with SPC72. CC -!- INTERACTION: CC P06704:CDC31; NbExp=1; IntAct=EBI-9491, EBI-4259; CC -!- SUBCELLULAR LOCATION: Cytoplasm, cytoskeleton, spindle pole body. CC --------------------------------------------------------------------------- CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms CC Distributed under the Creative Commons Attribution (CC BY 4.0) License CC --------------------------------------------------------------------------- DR EMBL; M15683; AAA34716.1; -; Genomic_DNA. DR EMBL; Z71464; CAA96082.1; -; Genomic_DNA. DR EMBL; AY692882; AAT92901.1; -; Genomic_DNA. DR EMBL; BK006947; DAA10365.1; -; Genomic_DNA. DR PIR; S63143; S63143. DR RefSeq; NP_014211.1; NM_001183026.1. DR PDB; 1OQP; NMR; -; B=239-257. DR PDBsum; 1OQP; -. DR ProteinModelPortal; P11927; -. DR DIP; DIP-2274N; -. DR IntAct; P11927; 6. DR MINT; MINT-551700; -. DR STRING; P11927; -. DR EnsemblFungi; YNL188W; YNL188W; YNL188W. DR GeneID; 855533; -. DR KEGG; sce:YNL188W; -. DR NMPDR; fig|4932.3.peg.5276; -. DR CYGD; YNL188w; -. DR SGD; S000005132; KAR1. DR eggNOG; fuNOG12922; -. DR OrthoDB; EOG473T22; -. DR PhylomeDB; P11927; -. DR NextBio; 979586; -. DR ArrayExpress; P11927; -. DR Genevestigator; P11927; -. DR GermOnline; YNL188W; Saccharomyces cerevisiae. DR GO; GO:0005825; C:half bridge of spindle pole body; IDA:SGD. DR GO; GO:0005874; C:microtubule; IEA:UniProtKB-KW. DR GO; GO:0005634; C:nucleus; IEA:UniProtKB-KW. DR GO; GO:0005515; F:protein binding; IPI:IntAct. DR GO; GO:0051301; P:cell division; IEA:UniProtKB-KW. DR GO; GO:0000742; P:karyogamy involved in conjugation with cellular fusion; IMP:SGD. DR GO; GO:0007067; P:mitosis; IEA:UniProtKB-KW. DR GO; GO:0007103; P:spindle pole body duplication in nuclear envelope; IMP:SGD. PE 1: Evidence at protein level; KW 3D-structure; Cell cycle; Cell division; Complete proteome; Cytoplasm; KW Cytoskeleton; Microtubule; Mitosis; Nucleus. FT CHAIN 1 433 Cell division control protein KAR1. FT /FTId=PRO_0000084295. FT CONFLICT 95 95 Y -> H (in Ref. 4; AAT92901). FT CONFLICT 199 199 A -> V (in Ref. 1; AAA34716). FT HELIX 240 252 SQ SEQUENCE 433 AA; 50653 MW; D65331B3B2B068E4 CRC64; MNVTSPKDGN HSFSKKNRFN TNKPRFHKLN EQAQSINLPE DRDSIVSSNT TSIMTDDAFD YNEGIASRTK NINSDSDRSN DTIKQNNYNK RETGYNPFYN GSGINQRYTQ FRKREFEPTL AENKAEEYIS DEDNVKIDED NIENELQFTP KIKEASILRS SLLGQRNVLN TRNPKSKESH IKVKPIINNK SSSQRKSSAA LRKQLGKPLP LPYLNSPNSD STPTLQRKEE VFTDEVLQKK RELIESKWHR LLFHDKKMVE KKLESLREYE RKRMPPRGTD VSSSEQDNSF KISTPTKSYV SLEQKPLPNL SAMNNFNDVT DNKEKEETNN NILKFQAQRD PLQILQSEIE MHTKKLDTII ELLKDDTDSK EKRKVVTNDN AAPEQMVNKG WRKNVMMIYK KSGNIMKKYR EYFLWTICIL ILLYCNIYVY YRF //