ID RCA_ARATH STANDARD; PRT; 474 AA. AC P10896; Q39197; Q39198; Q940T8; DT 01-JUL-1989 (Rel. 11, Created) DT 16-OCT-2001 (Rel. 40, Last sequence update) DT 05-JUL-2004 (Rel. 44, Last annotation update) DE Ribulose bisphosphate carboxylase/oxygenase activase, chloroplast DE precursor (RuBisCO activase) (RA). GN Name=RCA; OrderedLocusNames=At2g39730; ORFNames=T5I7.3; OS Arabidopsis thaliana (Mouse-ear cress). OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; OC Spermatophyta; Magnoliophyta; eudicotyledons; core eudicots; rosids; OC eurosids II; Brassicales; Brassicaceae; Arabidopsis. OX NCBI_TaxID=3702; RN [1] RP SEQUENCE FROM N.A. RC TISSUE=Leaf; RX MEDLINE=89240055; PubMed=2717419; RA Werneke J.M., Ogren W.L.; RT "Structure of an Arabidopsis thaliana cDNA encoding rubisco RT activase."; RL Nucleic Acids Res. 17:2871-2871(1989). RN [2] RP SEQUENCE FROM N.A. RX MEDLINE=94151424; PubMed=8108496; RA Orozco B.M., McClung C.R., Werneke J.M., Ogren W.L.; RT "Molecular basis of the ribulose-1,5-bisphosphate RT carboxylase/oxygenase activase mutation in Arabidopsis thaliana is a RT guanine-to-adenine transition at the 5'-splice junction of intron 3."; RL Plant Physiol. 102:227-232(1993). RN [3] RP SEQUENCE FROM N.A. RC STRAIN=cv. Columbia; RX MEDLINE=20083487; PubMed=10617197; RA Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D., RA Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V., RA Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L., RA Moffat K.S., Cronin L.A., Shen M., Pai G., Van Aken S., Umayam L., RA Tallon L.J., Gill J.E., Adams M.D., Carrera A.J., Creasy T.H., RA Goodman H.M., Somerville C.R., Copenhaver G.P., Preuss D., RA Nierman W.C., White O., Eisen J.A., Salzberg S.L., Fraser C.M., RA Venter J.C.; RT "Sequence and analysis of chromosome 2 of the plant Arabidopsis RT thaliana."; RL Nature 402:761-768(1999). RN [4] RP SEQUENCE FROM N.A. (ISOFORM SHORT). RC STRAIN=cv. Columbia; RX MEDLINE=22954850; PubMed=14593172; DOI=10.1126/science.1088305; RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., RA Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., RA Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G., RA Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., RA Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., RA Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., RA Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., RA Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., RA Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., RA Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., RA Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., RA Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.; RT "Empirical analysis of transcriptional activity in the Arabidopsis RT genome."; RL Science 302:842-846(2003). RN [5] RP ALTERNATIVE SPLICING. RX MEDLINE=92393414; PubMed=2535524; RA Werneke J.M., Chatfield J.M., Ogren W.L.; RT "Alternative mRNA splicing generates the two ribulosebisphosphate RT carboxylase/oxygenase activase polypeptides in spinach and RT Arabidopsis."; RL Plant Cell 1:815-825(1989). RN [6] RP SUBCELLULAR LOCATION. RX PubMed=12775770; DOI=10.1074/mcp.M300030-MCP200; RA Ferro M., Salvi D., Brugiere S., Miras S., Kowalski S., Louwagie M., RA Garin J., Joyard J., Rolland N.; RT "Proteomics of the chloroplast envelope membranes from Arabidopsis RT thaliana."; RL Mol. Cell. Proteomics 2:325-345(2003). CC -!- FUNCTION: Activation of RuBisCO (ribulose-1,5-bisphosphate CC carboxylase/oxygenase; EC 4.1.1.39) involves the ATP-dependent CC carboxylation of the epsilon-amino group of lysine leading to a CC carbamate structure. CC -!- SUBCELLULAR LOCATION: Chloroplast; stroma. CC -!- ALTERNATIVE PRODUCTS: CC Event=Alternative splicing; Named isoforms=2; CC Name=Long; CC IsoId=P10896-1; Sequence=Displayed; CC Name=Short; CC IsoId=P10896-2; Sequence=VSP_005539; CC -!- SIMILARITY: Belongs to the RuBisCO activase family. CC --------------------------------------------------------------------------- CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms CC Distributed under the Creative Commons Attribution (CC BY 4.0) License CC --------------------------------------------------------------------------- DR EMBL; X14212; CAA32429.1; -. DR EMBL; M86720; AAA20202.1; -. DR EMBL; M86720; AAA20203.1; -. DR EMBL; AC003000; AAB87122.1; -. DR EMBL; AY052703; AAK96607.1; -. DR EMBL; BT000710; AAN31853.1; -. DR PIR; S04048; S04048. DR PIR; T01002; T01002. DR SWISS-2DPAGE; P10896; ARATH. DR InterPro; IPR003959; AAA_ATPase_centr. DR InterPro; IPR009057; Homeodomain_like. DR Pfam; PF00004; AAA; 1. KW Chloroplast; Transit peptide; ATP-binding; Alternative splicing. FT TRANSIT 1 58 Chloroplast. FT CHAIN 59 474 Ribulose bisphosphate FT carboxylase/oxygenase activase. FT NP_BIND 165 172 ATP (Potential). FT VARSPLIC 439 474 GAQQVNLPVPEGCTDPVAENFDPTARSDDGTCVYNF -> T FT EEKEPSK (in isoform Short). FT /FTId=VSP_005539. FT CONFLICT 163 164 IW -> SR (in Ref. 1). FT CONFLICT 202 203 PA -> VR (in Ref. 1). FT CONFLICT 292 292 A -> G (in Ref. 1). FT CONFLICT 296 296 R -> L (in Ref. 1). FT CONFLICT 304 306 YWA -> LTG (in Ref. 1). FT CONFLICT 316 317 CK -> W (in Ref. 1). FT CONFLICT 441 442 QQ -> HE (in Ref. 1). SQ SEQUENCE 474 AA; 51981 MW; 4AD07691E1892A4F CRC64; MAAAVSTVGA INRAPLSLNG SGSGAVSAPA STFLGKKVVT VSRFAQSNKK SNGSFKVLAV KEDKQTDGDR WRGLAYDTSD DQQDITRGKG MVDSVFQAPM GTGTHHAVLS SYEYVSQGLR QYNLDNMMDG FYIAPAFMDK LVVHITKNFL TLPNIKVPLI LGIWGGKGQG KSFQCELVMA KMGINPIMMS AGELESGNAG EPAKLIRQRY REAADLIKKG KMCCLFINDL DAGAGRMGGT TQYTVNNQMV NATLMNIADN PTNVQLPGMY NKEENARVPI ICTGNDFSTL YAPLIRDGRM EKFYWAPTRE DRIGVCKGIF RTDKIKDEDI VTLVDQFPGQ SIDFFGALRA RVYDDEVRKF VESLGVEKIG KRLVNSREGP PVFEQPEMTY EKLMEYGNML VMEQENVKRV QLAETYLSQA ALGDANADAI GRGTFYGKGA QQVNLPVPEG CTDPVAENFD PTARSDDGTC VYNF //