ID BINB1_LYSSH Reviewed; 448 AA. AC P10565; DT 01-JUL-1989, integrated into UniProtKB/Swiss-Prot. DT 01-JUL-1989, sequence version 1. DT 23-MAY-2018, entry version 59. DE RecName: Full=Larvicidal toxin 51 kDa protein; DE AltName: Full=Strains 1593/2362; GN Name=binB; Synonyms=sph04; OS Lysinibacillus sphaericus (Bacillus sphaericus). OC Bacteria; Firmicutes; Bacilli; Bacillales; Bacillaceae; OC Lysinibacillus. OX NCBI_TaxID=1421; RN [1] RP NUCLEOTIDE SEQUENCE [GENOMIC DNA]. RC STRAIN=ATCC 33203 / 1593; RX PubMed=3412905; DOI=10.1093/nar/16.15.7731; RA Arapinis C., de la Torre F., Szulmajster J.; RT "Nucleotide and deduced amino acid sequence of the Bacillus sphaericus RT 1593M gene encoding a 51.4 kD polypeptide which acts synergistically RT with the 42 kD protein for expression of the larvicidal toxin."; RL Nucleic Acids Res. 16:7731-7731(1988). RN [2] RP NUCLEOTIDE SEQUENCE [GENOMIC DNA]. RC STRAIN=2362; RX PubMed=3360740; DOI=10.1128/jb.170.5.2045-2050.1988; RA Baumann L., Broadwell A.H., Baumann P.; RT "Sequence analysis of the mosquitocidal toxin genes encoding 51.4- and RT 41.9-kilodalton proteins from Bacillus sphaericus 2362 and 2297."; RL J. Bacteriol. 170:2045-2050(1988). RN [3] RP NUCLEOTIDE SEQUENCE [GENOMIC DNA]. RC STRAIN=ATCC 33203 / 1593; RA Humphreys M.J., Coleman M.M., Berry C.; RT "Transposition of Bacillus sphaericus toxin genes."; RL Submitted (FEB-1998) to the EMBL/GenBank/DDBJ databases. CC -!- FUNCTION: Binding component of binary toxin. The 51 kDa CC polypeptide acts synergetically with the 42 kDa polypeptide for CC expression of a larvicidal toxin. CC --------------------------------------------------------------------------- CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms CC Distributed under the Creative Commons Attribution (CC BY 4.0) License CC --------------------------------------------------------------------------- DR EMBL; X07992; CAA30800.1; -; Genomic_DNA. DR EMBL; M20390; AAA22860.1; -; Genomic_DNA. DR EMBL; AJ224477; CAB37654.1; -; Genomic_DNA. DR PIR; A28211; A28211. DR RefSeq; WP_012291792.1; NZ_LWHI01000001.1. DR PDB; 5FOY; X-ray; 2.25 A; B=1-448. DR PDB; 5FOZ; X-ray; 2.40 A; B=1-448. DR PDB; 5G37; X-ray; 2.50 A; B=1-448. DR PDBsum; 5FOY; -. DR PDBsum; 5FOZ; -. DR PDBsum; 5G37; -. DR ProteinModelPortal; P10565; -. DR SMR; P10565; -. DR TCDB; 1.C.4.7.1; the aerolysin channel-forming toxin (aerolysin) family. DR PRIDE; P10565; -. DR GO; GO:0090729; F:toxin activity; IEA:UniProtKB-KW. DR GO; GO:0030435; P:sporulation resulting in formation of a cellular spore; IEA:UniProtKB-KW. DR InterPro; IPR008872; Toxin_P42. DR Pfam; PF05431; Toxin_10; 1. PE 1: Evidence at protein level; KW 3D-structure; Sporulation; Toxin. FT CHAIN 1 448 Larvicidal toxin 51 kDa protein. FT /FTId=PRO_0000174114. FT HELIX 36 38 {ECO:0000244|PDB:5FOY}. FT STRAND 39 48 {ECO:0000244|PDB:5FOY}. FT STRAND 54 56 {ECO:0000244|PDB:5FOY}. FT STRAND 71 73 {ECO:0000244|PDB:5FOY}. FT STRAND 75 81 {ECO:0000244|PDB:5FOY}. FT STRAND 84 88 {ECO:0000244|PDB:5FOY}. FT STRAND 94 98 {ECO:0000244|PDB:5FOY}. FT TURN 99 101 {ECO:0000244|PDB:5FOY}. FT STRAND 104 107 {ECO:0000244|PDB:5FOY}. FT HELIX 113 115 {ECO:0000244|PDB:5FOZ}. FT STRAND 117 122 {ECO:0000244|PDB:5FOY}. FT HELIX 128 130 {ECO:0000244|PDB:5FOY}. FT STRAND 131 138 {ECO:0000244|PDB:5FOY}. FT HELIX 140 142 {ECO:0000244|PDB:5FOY}. FT STRAND 146 151 {ECO:0000244|PDB:5FOY}. FT TURN 152 155 {ECO:0000244|PDB:5FOY}. FT STRAND 156 162 {ECO:0000244|PDB:5FOY}. FT STRAND 164 166 {ECO:0000244|PDB:5FOY}. FT STRAND 168 174 {ECO:0000244|PDB:5FOY}. FT TURN 179 182 {ECO:0000244|PDB:5FOY}. FT HELIX 185 189 {ECO:0000244|PDB:5FOY}. FT STRAND 191 198 {ECO:0000244|PDB:5FOY}. FT STRAND 218 220 {ECO:0000244|PDB:5G37}. FT HELIX 226 228 {ECO:0000244|PDB:5FOY}. FT STRAND 232 240 {ECO:0000244|PDB:5FOY}. FT HELIX 241 243 {ECO:0000244|PDB:5FOY}. FT HELIX 251 257 {ECO:0000244|PDB:5FOY}. FT STRAND 259 277 {ECO:0000244|PDB:5FOY}. FT STRAND 282 290 {ECO:0000244|PDB:5FOY}. FT HELIX 293 303 {ECO:0000244|PDB:5FOY}. FT STRAND 304 308 {ECO:0000244|PDB:5FOY}. FT STRAND 313 315 {ECO:0000244|PDB:5FOY}. FT HELIX 317 320 {ECO:0000244|PDB:5FOY}. FT HELIX 323 330 {ECO:0000244|PDB:5FOY}. FT STRAND 343 351 {ECO:0000244|PDB:5FOY}. FT STRAND 354 356 {ECO:0000244|PDB:5FOY}. FT STRAND 358 372 {ECO:0000244|PDB:5FOY}. FT STRAND 378 396 {ECO:0000244|PDB:5FOY}. FT TURN 398 400 {ECO:0000244|PDB:5FOY}. FT STRAND 404 413 {ECO:0000244|PDB:5FOY}. FT STRAND 425 429 {ECO:0000244|PDB:5FOY}. FT HELIX 437 439 {ECO:0000244|PDB:5FOY}. FT STRAND 442 444 {ECO:0000244|PDB:5FOY}. SQ SEQUENCE 448 AA; 51431 MW; 55112D0131BCE018 CRC64; MCDSKDNSGV SEKCGKKFTN YPLNTTPTSL NYNLPEISKK FYNLKNKYSR NGYGLSKTEF PSSIENCPSN EYSIMYDNKD PRFLIRFLLD DGRYIIADRD DGEVFDEAPT YLDNNNHPII SRHYTGEERQ KFEQVGSGDY ITGEQFFQFY TQNKTRVLSN CRALDSRTIL LSTAKIFPIY PPASETQLTA FVNSSFYAAA IPQLPQTSLL ENIPEPTSLD DSGVLPKDAV RAVKGSALLP CIIVHDPNLN NSDKMKFNTY YLLEYKEYWH QLWSQIIPAH QTVKIQERTG ISEVVQNSMI EDLNMYIGAD FGMLFYFRSS GFKEQITRGL NRPLSQTTTQ LGERVEEMEY YNSNDLDVRY VKYALAREFT LKRVNGEIVK NWVAVDYRLA GIQSYPNAPI TNPLTLTKHT IIRCENSYDG HIFKTPLIFK NGEVIVKTNE ELIPKINQ //