ID FAPF_PSEFL Reviewed; 430 AA. AC P0DXF8; DT 27-NOV-2024, integrated into UniProtKB/Swiss-Prot. DT 27-NOV-2024, sequence version 1. DT 27-NOV-2024, entry version 1. DE RecName: Full=Probable fibril transporter FapF {ECO:0000303|PubMed:23504942}; DE Flags: Precursor; GN Name=fapF {ECO:0000303|PubMed:20572935}; GN ORFNames=HZ99_04105 {ECO:0000303|PubMed:25212622}, GN PSUK4_00060 {ECO:0000303|PubMed:20572935}; OS Pseudomonas fluorescens. OC Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales; OC Pseudomonadaceae; Pseudomonas. OX NCBI_TaxID=294; RN [1] RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND FUNCTION. RC STRAIN=UK4; RX PubMed=20572935; DOI=10.1111/j.1365-2958.2010.07269.x; RA Dueholm M.S., Petersen S.V., Soenderkaer M., Larsen P., Christiansen G., RA Hein K.L., Enghild J.J., Nielsen J.L., Nielsen K.L., Nielsen P.H., RA Otzen D.E.; RT "Functional amyloid in Pseudomonas."; RL Mol. Microbiol. 77:1009-1020(2010). RN [2] RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. RC STRAIN=UK4; RX PubMed=25212622; DOI=10.1128/genomea.00898-14; RA Dueholm M.S., Danielsen H.N., Nielsen P.H.; RT "Complete Genome Sequence of Pseudomonas sp. UK4, a Model Organism for RT Studies of Functional Amyloids in Pseudomonas."; RL Genome Announc. 2:0-0(2014). RN [3] RP FUNCTION, POSSIBLE SUBCELLULAR LOCATION, AND DISRUPTION PHENOTYPE. RC STRAIN=UK4; RX PubMed=23504942; DOI=10.1002/mbo3.81; RA Dueholm M.S., Soendergaard M.T., Nilsson M., Christiansen G., RA Stensballe A., Overgaard M.T., Givskov M., Tolker-Nielsen T., Otzen D.E., RA Nielsen P.H.; RT "Expression of Fap amyloids in Pseudomonas aeruginosa, P. fluorescens, and RT P. putida results in aggregation and increased biofilm formation."; RL MicrobiologyOpen 2:365-382(2013). RN [4] RP FUNCTION, AND DISRUPTION PHENOTYPE. RC STRAIN=UK4; RX PubMed=26500638; DOI=10.3389/fmicb.2015.01099; RA Zeng G., Vad B.S., Dueholm M.S., Christiansen G., Nilsson M., RA Tolker-Nielsen T., Nielsen P.H., Meyer R.L., Otzen D.E.; RT "Functional bacterial amyloid increases Pseudomonas biofilm hydrophobicity RT and stiffness."; RL Front. Microbiol. 6:1099-1099(2015). CC -!- FUNCTION: Probably transports fibril components across the outer CC membrane (Probable) (PubMed:23504942). Upon overexpression of the CC endogenous six-gene locus (fapA-fapF) in situ cells form large clumps CC during liquid growth, make large amounts of biofilm and produce amyloid CC fibrils (PubMed:23504942, PubMed:26500638). Expression of the 6 gene CC operon in E.coli strain BL21(DE3) induces flocculation and biofilm CC formation with copious extracellular fibrils (PubMed:20572935). CC {ECO:0000269|PubMed:20572935, ECO:0000269|PubMed:23504942, CC ECO:0000269|PubMed:26500638}. CC -!- SUBCELLULAR LOCATION: Cell outer membrane CC {ECO:0000305|PubMed:23504942}. CC -!- DISRUPTION PHENOTYPE: Deletion of just fapF in an overexpressing strain CC yields cells without amyloid fibrils (PubMed:26500638). Deletion of the CC entire fapA-fapF six-gene locus shows no visible growth phenotype CC (PubMed:23504942, PubMed:26500638). {ECO:0000269|PubMed:23504942, CC ECO:0000269|PubMed:26500638}. CC -!- SIMILARITY: Belongs to the amyloid transporter (TC 9.B.153) family. CC {ECO:0000305}. CC --------------------------------------------------------------------------- CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms CC Distributed under the Creative Commons Attribution (CC BY 4.0) License CC --------------------------------------------------------------------------- DR EMBL; ACOQ01000001; EEP64554.1; -; Genomic_DNA. DR EMBL; CP008896; AIG01394.1; -; Genomic_DNA. PE 3: Inferred from homology; KW Cell outer membrane; Coiled coil; Membrane; Protein transport; Signal; KW Transport. FT SIGNAL 1..24 FT /evidence="ECO:0000255" FT CHAIN 25..430 FT /note="Probable fibril transporter FapF" FT /evidence="ECO:0000255" FT /id="PRO_0000461518" FT COILED 30..64 FT /evidence="ECO:0000255" SQ SEQUENCE 430 AA; 46467 MW; E364465EB59EA9D0 CRC64; MHRSLSLRAV VCLSTLLPAS LLYAAPDVDI ETLKQELLEL KQRYEAQQKA LAVLEQRVRQ VEDQPATPAP KRLAKSPADF KQSGSTVAAS SGTGGATGGS SYGQSLKDDS EPAQSVSNLY NEASGFFGNG KFSFETGITY ARYDARQLTL NGFLALDSIF LGNINLDRIK ADNWTLDLTG RYNLDNRWQF DVNVPVVYRE STYQSGGASG GDPQATSEES VSRDPTIGDV NFGIAYKFLD ESATMPDAVV SVRVKAPTGK EPFGIKLVRS TANDNLYVPE SLPTGNGVWS ITPGLSLVKT FDPAVLFGSV SYTHNLEDSF DDISSDVNQK VGGKVRLGDS FQFGVGVAFA LNERMSMSFS VSDLIQRKSK LKPDGGGWQS IVSSDANAGY FNVGMTIAAS ENLTIVPNLA IGMTDDAPDF TFSLKFPYYF //