ID ESXV_MYCTU Reviewed; 94 AA. AC P0DOA7; L0TEQ6; O08120; O08122; P96364; P9WNK1; Q9L781; DT 07-SEP-2016, integrated into UniProtKB/Swiss-Prot. DT 07-SEP-2016, sequence version 1. DT 13-FEB-2019, entry version 12. DE RecName: Full=ESAT-6-like protein EsxV {ECO:0000305}; DE AltName: Full=Antigen Mtb9.9B; GN Name=esxV {ECO:0000303|PubMed:19876390}; OrderedLocusNames=Rv3619c; GN ORFNames=MTCY07H7B.03, MTCY15C10.33; OS Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv). OC Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae; OC Mycobacterium; Mycobacterium tuberculosis complex. OX NCBI_TaxID=83332; RN [1] RP NUCLEOTIDE SEQUENCE [GENOMIC DNA]. RC STRAIN=ATCC 35801 / TMC 107 / Erdman; RX PubMed=10662800; DOI=10.1084/jem.191.3.551; RA Alderson M.R., Bement T., Day C.H., Zhu L., Molesh D., Skeiky Y.A.W., RA Coler R., Lewinsohn D.M., Reed S.G., Dillon D.C.; RT "Expression cloning of an immunodominant family of Mycobacterium RT tuberculosis antigens using human CD4(+) T cells."; RL J. Exp. Med. 191:551-560(2000). RN [2] RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. RC STRAIN=ATCC 25618 / H37Rv; RX PubMed=9634230; DOI=10.1038/31159; RA Cole S.T., Brosch R., Parkhill J., Garnier T., Churcher C.M., RA Harris D.E., Gordon S.V., Eiglmeier K., Gas S., Barry C.E. III, RA Tekaia F., Badcock K., Basham D., Brown D., Chillingworth T., RA Connor R., Davies R.M., Devlin K., Feltwell T., Gentles S., Hamlin N., RA Holroyd S., Hornsby T., Jagels K., Krogh A., McLean J., Moule S., RA Murphy L.D., Oliver S., Osborne J., Quail M.A., Rajandream M.A., RA Rogers J., Rutter S., Seeger K., Skelton S., Squares S., Squares R., RA Sulston J.E., Taylor K., Whitehead S., Barrell B.G.; RT "Deciphering the biology of Mycobacterium tuberculosis from the RT complete genome sequence."; RL Nature 393:537-544(1998). RN [3] RP NOMENCLATURE. RX PubMed=19876390; DOI=10.1371/journal.ppat.1000507; RA Bitter W., Houben E.N., Bottai D., Brodin P., Brown E.J., Cox J.S., RA Derbyshire K., Fortune S.M., Gao L.Y., Liu J., Gey van Pittius N.C., RA Pym A.S., Rubin E.J., Sherman D.R., Cole S.T., Brosch R.; RT "Systematic genetic nomenclature for type VII secretion systems."; RL PLoS Pathog. 5:E1000507-E1000507(2009). RN [4] RP FUNCTION IN VIRULENCE, INTERACTION WITH ESXW, AND SUBCELLULAR RP LOCATION. RC STRAIN=H37Rv; RX PubMed=21134129; DOI=10.1111/j.1742-4658.2010.07958.x; RA Mahmood A., Srivastava S., Tripathi S., Ansari M.A., Owais M., RA Arora A.; RT "Molecular characterization of secretory proteins Rv3619c and Rv3620c RT from Mycobacterium tuberculosis H37Rv."; RL FEBS J. 278:341-353(2011). CC -!- FUNCTION: Probable virulence factor. In mice, elicits increased CC levels of IFN-gamma, IL-12 and IgG(2a), indicating a dominant Th1 CC host immune response. {ECO:0000269|PubMed:21134129}. CC -!- SUBUNIT: Forms a tight 1:1 complex with EsxW. The complex is CC destabilized at low pH. Unfolding of the proteins is required for CC dissociation of the complex and membrane binding. CC {ECO:0000269|PubMed:21134129}. CC -!- SUBCELLULAR LOCATION: Secreted {ECO:0000305|PubMed:19876390}. CC Note=Probably secreted via the ESX-5 / type VII secretion system CC (T7SS) (Probable). May bind to lipid membranes after dissociation CC of the complex (Probable). {ECO:0000305|PubMed:19876390, CC ECO:0000305|PubMed:21134129}. CC -!- MISCELLANEOUS: Potent T-cell antigen that could be a subunit CC vaccine candidate. {ECO:0000305|PubMed:21134129}. CC -!- SIMILARITY: Belongs to the WXG100 family. ESAT-6 subfamily. CC {ECO:0000305|PubMed:19876390}. CC --------------------------------------------------------------------------- CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms CC Distributed under the Creative Commons Attribution (CC BY 4.0) License CC --------------------------------------------------------------------------- DR EMBL; AF226277; AAF32406.1; -; Genomic_DNA. DR EMBL; AL123456; CCP46442.1; -; Genomic_DNA. DR PIR; D70560; D70560. DR RefSeq; NP_215553.1; NC_000962.3. DR RefSeq; NP_218136.1; NC_000962.3. DR RefSeq; WP_003898699.1; NZ_NVQJ01000149.1. DR ProteinModelPortal; P0DOA7; -. DR SMR; P0DOA7; -. DR EnsemblBacteria; CCP46442; CCP46442; Rv3619c. DR GeneID; 885328; -. DR GeneID; 888299; -. DR KEGG; mtu:Rv1037c; -. DR KEGG; mtu:Rv3619c; -. DR TubercuList; Rv3619c; -. DR PhylomeDB; P0DOA7; -. DR BioCyc; MTBH37RV:G185E-7898-MONOMER; -. DR Proteomes; UP000001584; Chromosome. DR GO; GO:0005618; C:cell wall; IDA:UniProtKB. DR GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell. DR GO; GO:0009405; P:pathogenesis; IEA:UniProtKB-KW. DR InterPro; IPR009416; ESAT-6-like_Myco. DR InterPro; IPR036689; ESAT-6-like_sf. DR InterPro; IPR010310; T7SS_ESAT-6-like. DR Pfam; PF06013; WXG100; 1. DR PIRSF; PIRSF037656; DUF1066; 1. DR SUPFAM; SSF140453; SSF140453; 1. PE 1: Evidence at protein level; KW Complete proteome; Reference proteome; Secreted; Virulence. FT CHAIN 1 94 ESAT-6-like protein EsxV. FT /FTId=PRO_0000436928. FT CONFLICT 20 20 Q -> L (in Ref. 1; AAF32406). FT {ECO:0000305}. FT CONFLICT 23 23 S -> L (in Ref. 1; AAF32406). FT {ECO:0000305}. SQ SEQUENCE 94 AA; 9833 MW; 8F971BB03789C57E CRC64; MTINYQFGDV DAHGAMIRAQ AGSLEAEHQA IISDVLTASD FWGGAGSAAC QGFITQLGRN FQVIYEQANA HGQKVQAAGN NMAQTDSAVG SSWA //