ID HMAL2_YEAST Reviewed; 210 AA. AC P0CY09; D6VQV2; P01367; P01368; Q6B2C0; DT 28-JUN-2011, integrated into UniProtKB/Swiss-Prot. DT 28-JUN-2011, sequence version 1. DT 21-MAR-2012, entry version 8. DE RecName: Full=Silenced mating-type protein ALPHA2; DE Short=MATalpha2 protein; DE AltName: Full=Alpha-2 repressor; GN Name=HMLALPHA2; OrderedLocusNames=YCL067C; ORFNames=YCL67C; OS Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast). OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; OC Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces. OX NCBI_TaxID=559292; RN [1] RP NUCLEOTIDE SEQUENCE [GENOMIC DNA]. RX MEDLINE=82115330; PubMed=7034964; DOI=10.1016/0092-8674(81)90356-1; RA Astell C.R., Ahlstrom-Jonasson L., Smith M., Tatchell K., RA Nasmyth K.A., Hall B.D.; RT "The sequence of the DNAs coding for the mating-type loci of RT Saccharomyces cerevisiae."; RL Cell 27:15-23(1981). RN [2] RP NUCLEOTIDE SEQUENCE [GENOMIC DNA]. RX MEDLINE=82115339; PubMed=6276023; DOI=10.1016/0092-8674(81)90357-3; RA Tatchell K., Nasmyth K.A., Hall B.D., Astell C.R., Smith M.; RT "In vitro mutation analysis of the mating-type locus in yeast."; RL Cell 27:25-35(1981). RN [3] RP NUCLEOTIDE SEQUENCE [GENOMIC DNA]. RX PubMed=7021055; RA Nasmyth K.A., Tatchell K., Hall B.D., Astell C., Smith M.; RT "Physical analysis of mating-type loci in Saccharomyces cerevisiae."; RL Cold Spring Harb. Symp. Quant. Biol. 45:961-981(1981). RN [4] RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. RC STRAIN=ATCC 204508 / S288c; RX MEDLINE=92244356; PubMed=1574125; DOI=10.1038/357038a0; RA Oliver S.G., van der Aart Q.J.M., Agostoni-Carbone M.L., Aigle M., RA Alberghina L., Alexandraki D., Antoine G., Anwar R., Ballesta J.P.G., RA Benit P., Berben G., Bergantino E., Biteau N., Bolle P.-A., RA Bolotin-Fukuhara M., Brown A., Brown A.J.P., Buhler J.-M., Carcano C., RA Carignani G., Cederberg H., Chanet R., Contreras R., Crouzet M., RA Daignan-Fornier B., Defoor E., Delgado M.D., Demolder J., Doira C., RA Dubois E., Dujon B., Duesterhoeft A., Erdmann D., Esteban M., RA Fabre F., Fairhead C., Faye G., Feldmann H., Fiers W., RA Francingues-Gaillard M.-C., Franco L., Frontali L., Fukuhara H., RA Fuller L.J., Galland P., Gent M.E., Gigot D., Gilliquet V., RA Glansdorff N., Goffeau A., Grenson M., Grisanti P., Grivell L.A., RA de Haan M., Haasemann M., Hatat D., Hoenicka J., Hegemann J.H., RA Herbert C.J., Hilger F., Hohmann S., Hollenberg C.P., Huse K., RA Iborra F., Indge K.J., Isono K., Jacq C., Jacquet M., James C.M., RA Jauniaux J.-C., Jia Y., Jimenez A., Kelly A., Kleinhans U., Kreisl P., RA Lanfranchi G., Lewis C., van der Linden C.G., Lucchini G., RA Lutzenkirchen K., Maat M.J., Mallet L., Mannhaupt G., Martegani E., RA Mathieu A., Maurer C.T.C., McConnell D., McKee R.A., Messenguy F., RA Mewes H.-W., Molemans F., Montague M.A., Muzi Falconi M., Navas L., RA Newlon C.S., Noone D., Pallier C., Panzeri L., Pearson B.M., Perea J., RA Philippsen P., Pierard A., Planta R.J., Plevani P., Poetsch B., RA Pohl F.M., Purnelle B., Ramezani Rad M., Rasmussen S.W., Raynal A., RA Remacha M.A., Richterich P., Roberts A.B., Rodriguez F., Sanz E., RA Schaaff-Gerstenschlaeger I., Scherens B., Schweitzer B., Shu Y., RA Skala J., Slonimski P.P., Sor F., Soustelle C., Spiegelberg R., RA Stateva L.I., Steensma H.Y., Steiner S., Thierry A., Thireos G., RA Tzermia M., Urrestarazu L.A., Valle G., Vetter I., RA van Vliet-Reedijk J.C., Voet M., Volckaert G., Vreken P., Wang H., RA Warmington J.R., von Wettstein D., Wicksteed B.L., Wilson C., RA Wurst H., Xu G., Yoshikawa A., Zimmermann F.K., Sgouros J.G.; RT "The complete DNA sequence of yeast chromosome III."; RL Nature 357:38-46(1992). RN [5] RP GENOME REANNOTATION. RC STRAIN=ATCC 204508 / S288c; RG Saccharomyces Genome Database; RL Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases. RN [6] RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-9. RX MEDLINE=81099018; PubMed=6256656; DOI=10.1038/289244a0; RA Nasmyth K.A., Tatchell K., Hall B.D., Astell C.R., Smith M.; RT "A position effect in the control of transcription at yeast mating RT type loci."; RL Nature 289:244-250(1981). CC -!- FUNCTION: Mating type proteins are sequence specific DNA-binding CC proteins that act as master switches in yeast differentiation by CC controlling gene expression in a cell type-specific fashion. CC Silenced copy of ALPHA2 at HML. CC -!- SUBCELLULAR LOCATION: Nucleus. CC -!- MISCELLANEOUS: There are three genetic loci for mating type genes CC in S.cerevisiae. MAT is the expression locus that determines the CC mating type of the cell, whereas HML (containing HMLALPHA1 and CC HMLALPHA2) and HMR (containing HMRA1 and HMRA2) represent silenced CC repositories of mating type information. The mating type is CC determined by the MAT locus, which contains either a copy of HML CC or of HMR. Diploid cells are usually heterozygous for the MAT CC locus. CC -!- SIMILARITY: Belongs to the TALE/M-ATYP homeobox family. CC -!- SIMILARITY: Contains 1 homeobox DNA-binding domain. CC --------------------------------------------------------------------------- CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms CC Distributed under the Creative Commons Attribution (CC BY 4.0) License CC --------------------------------------------------------------------------- DR EMBL; V01315; CAA24625.1; -; Genomic_DNA. DR EMBL; L00059; AAA34678.1; -; Genomic_DNA. DR EMBL; X59720; CAA42400.1; -; Genomic_DNA. DR EMBL; V01314; CAA24623.1; -; Genomic_DNA. DR EMBL; BK006937; DAA07421.1; -; Genomic_DNA. DR PIR; S19398; JFBYA2. DR RefSeq; NP_009866.1; NM_001178708.1. DR RefSeq; NP_009868.3; NM_001178753.1. DR ProteinModelPortal; P0CY09; -. DR SMR; P0CY09; 128-205. DR EnsemblFungi; YCL067C; YCL067C; YCL067C. DR EnsemblFungi; YCR039C; YCR039C; YCR039C. DR GeneID; 850292; -. DR GeneID; 850406; -. DR KEGG; sce:YCL067C; -. DR KEGG; sce:YCR039C; -. DR CYGD; YCL067c; -. DR SGD; S000000572; HMLALPHA2. DR GeneTree; ENSGT00390000002531; -. DR KO; K09359; -. DR NextBio; 965658; -. DR GermOnline; YCL067C; Saccharomyces cerevisiae. DR GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell. DR GO; GO:0043565; F:sequence-specific DNA binding; IEA:InterPro. DR GO; GO:0003700; F:sequence-specific DNA binding transcription factor activity; IEA:InterPro. DR GO; GO:0006351; P:transcription, DNA-dependent; IEA:UniProtKB-KW. DR InterPro; IPR001356; Homeodomain. DR InterPro; IPR009057; Homeodomain-like. DR Gene3D; G3DSA:1.10.10.60; Homeodomain-rel; 1. DR Pfam; PF00046; Homeobox; 1. DR SMART; SM00389; HOX; 1. DR SUPFAM; SSF46689; Homeodomain_like; 1. DR PROSITE; PS00027; HOMEOBOX_1; FALSE_NEG. DR PROSITE; PS50071; HOMEOBOX_2; 1. PE 3: Inferred from homology; KW Acetylation; Complete proteome; DNA-binding; Homeobox; Nucleus; KW Reference proteome; Repressor; Transcription; KW Transcription regulation. FT CHAIN 1 210 Silenced mating-type protein ALPHA2. FT /FTId=PRO_0000410464. FT DNA_BIND 129 191 Homeobox; TALE-type. FT REGION 1 102 N-terminal domain. FT REGION 103 128 Flexible linker. FT REGION 190 210 C-terminal tail. FT MOD_RES 1 1 N-acetylmethionine (By similarity). FT CONFLICT 56 56 G -> V (in Ref. 1; CAA24625, 2 and 3; FT AAA34678). SQ SEQUENCE 210 AA; 24282 MW; 30CD6A486D7D76CE CRC64; MNKIPIKDLL NPQITDEFKS SILDINKKLF SICCNLPKLP ESVTTEEEVE LRDILGFLSR ANKNRKISDE EKKLLQTTSQ LTTTITVLLK EMRSIENDRS NYQLTQKNKS ADGLVFNVVT QDMINKSTKP YRGHRFTKEN VRILESWFAK NIENPYLDTK GLENLMKNTS LSRIQIKNWV SNRRRKEKTI TIAPELADLL SGEPLAKKKE //