ID RS23A_SCHPO Reviewed; 143 AA. AC P0CT75; P79057; DT 24-JUN-2015, integrated into UniProtKB/Swiss-Prot. DT 24-JUN-2015, sequence version 1. DT 29-MAY-2024, entry version 44. DE RecName: Full=Small ribosomal subunit protein uS12A {ECO:0000305}; DE AltName: Full=40S ribosomal protein S23-A; GN Name=rps23; Synonyms=rps23a; ORFNames=SPAC23C11.02c; OS Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast). OC Eukaryota; Fungi; Dikarya; Ascomycota; Taphrinomycotina; OC Schizosaccharomycetes; Schizosaccharomycetales; Schizosaccharomycetaceae; OC Schizosaccharomyces. OX NCBI_TaxID=284812; RN [1] RP NUCLEOTIDE SEQUENCE [MRNA]. RA Kawamukai M.; RT "S.pombe ribosomal protein S23 homolog."; RL Submitted (FEB-1997) to the EMBL/GenBank/DDBJ databases. RN [2] RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. RC STRAIN=972 / ATCC 24843; RX PubMed=11859360; DOI=10.1038/nature724; RA Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A., RA Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S., RA Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M., RA Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S., RA Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S., RA Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D., RA Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P., RA Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K., RA O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M., RA Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N., RA Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A., RA Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R., RA Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M., RA Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A., RA Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A., RA Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H., RA Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S., RA Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C., RA Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A., RA Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M., RA del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S., RA Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R., RA Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G., RA Nurse P.; RT "The genome sequence of Schizosaccharomyces pombe."; RL Nature 415:871-880(2002). RN [3] RP SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS]. RX PubMed=16823372; DOI=10.1038/nbt1222; RA Matsuyama A., Arai R., Yashiroda Y., Shirai A., Kamata A., Sekido S., RA Kobayashi Y., Hashimoto A., Hamamoto M., Hiraoka Y., Horinouchi S., RA Yoshida M.; RT "ORFeome cloning and global analysis of protein localization in the fission RT yeast Schizosaccharomyces pombe."; RL Nat. Biotechnol. 24:841-847(2006). RN [4] RP HYDROXYLATION AT PRO-62. RX PubMed=24550462; DOI=10.1073/pnas.1311750111; RA Loenarz C., Sekirnik R., Thalhammer A., Ge W., Spivakovsky E., RA Mackeen M.M., McDonough M.A., Cockman M.E., Kessler B.M., Ratcliffe P.J., RA Wolf A., Schofield C.J.; RT "Hydroxylation of the eukaryotic ribosomal decoding center affects RT translational accuracy."; RL Proc. Natl. Acad. Sci. U.S.A. 111:4019-4024(2014). CC -!- FUNCTION: Component of the ribosome, a large ribonucleoprotein complex CC responsible for the synthesis of proteins in the cell. The small CC ribosomal subunit (SSU) binds messenger RNAs (mRNAs) and translates the CC encoded message by selecting cognate aminoacyl-transfer RNA (tRNA) CC molecules. The large subunit (LSU) contains the ribosomal catalytic CC site termed the peptidyl transferase center (PTC), which catalyzes the CC formation of peptide bonds, thereby polymerizing the amino acids CC delivered by tRNAs into a polypeptide chain. The nascent polypeptides CC leave the ribosome through a tunnel in the LSU and interact with CC protein factors that function in enzymatic processing, targeting, and CC the membrane insertion of nascent chains at the exit of the ribosomal CC tunnel. {ECO:0000250|UniProtKB:P0CX29}. CC -!- SUBUNIT: Component of the small ribosomal subunit (SSU). Mature yeast CC ribosomes consist of a small (40S) and a large (60S) subunit. The 40S CC small subunit contains 1 molecule of ribosomal RNA (18S rRNA) and at CC least 33 different proteins. The large 60S subunit contains 3 rRNA CC molecules (25S, 5.8S and 5S rRNA) and at least 46 different proteins. CC {ECO:0000250|UniProtKB:P0CX29}. CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250|UniProtKB:P0CX29}. Nucleus CC {ECO:0000269|PubMed:16823372}. Nucleus, nucleolus CC {ECO:0000269|PubMed:16823372}. CC -!- PTM: Hydroxylation at Pro-62 affects translation termination CC efficiency. {ECO:0000305|PubMed:24550462}. CC -!- MISCELLANEOUS: There are 2 genes for uS12 in S.pombe. CC -!- SIMILARITY: Belongs to the universal ribosomal protein uS12 family. CC {ECO:0000305}. CC -!- SEQUENCE CAUTION: CC Sequence=BAA19233.1; Type=Erroneous initiation; Evidence={ECO:0000305}; CC --------------------------------------------------------------------------- CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms CC Distributed under the Creative Commons Attribution (CC BY 4.0) License CC --------------------------------------------------------------------------- DR EMBL; AB001288; BAA19233.1; ALT_INIT; mRNA. DR EMBL; CU329670; CAB11155.1; -; Genomic_DNA. DR PIR; T38240; T38240. DR RefSeq; NP_593633.1; NM_001019064.2. DR RefSeq; NP_596187.1; NM_001022106.2. DR AlphaFoldDB; P0CT75; -. DR SMR; P0CT75; -. DR STRING; 284812.P0CT75; -. DR iPTMnet; P0CT75; -. DR PaxDb; 4896-SPAC23C11-02c-1; -. DR EnsemblFungi; SPAC23C11.02c.1; SPAC23C11.02c.1:pep; SPAC23C11.02c. DR EnsemblFungi; SPBP4H10.13.1; SPBP4H10.13.1:pep; SPBP4H10.13. DR GeneID; 2541369; -. DR GeneID; 2542012; -. DR KEGG; spo:SPAC23C11.02c; -. DR KEGG; spo:SPBP4H10.13; -. DR PomBase; SPAC23C11.02c; rps23. DR VEuPathDB; FungiDB:SPAC23C11.02c; -. DR VEuPathDB; FungiDB:SPBP4H10.13; -. DR eggNOG; KOG1749; Eukaryota. DR InParanoid; P0CT75; -. DR OMA; KFRWSQR; -. DR PhylomeDB; P0CT75; -. DR Reactome; R-SPO-156827; L13a-mediated translational silencing of Ceruloplasmin expression. DR Reactome; R-SPO-1799339; SRP-dependent cotranslational protein targeting to membrane. DR Reactome; R-SPO-72649; Translation initiation complex formation. DR Reactome; R-SPO-72689; Formation of a pool of free 40S subunits. DR Reactome; R-SPO-72695; Formation of the ternary complex, and subsequently, the 43S complex. DR Reactome; R-SPO-72702; Ribosomal scanning and start codon recognition. DR Reactome; R-SPO-72706; GTP hydrolysis and joining of the 60S ribosomal subunit. DR Reactome; R-SPO-9629569; Protein hydroxylation. DR Reactome; R-SPO-975956; Nonsense Mediated Decay (NMD) independent of the Exon Junction Complex (EJC). DR Reactome; R-SPO-975957; Nonsense Mediated Decay (NMD) enhanced by the Exon Junction Complex (EJC). DR PRO; PR:P0CT75; -. DR Proteomes; UP000002485; Chromosome I. DR GO; GO:0022627; C:cytosolic small ribosomal subunit; IBA:GO_Central. DR GO; GO:0005730; C:nucleolus; HDA:PomBase. DR GO; GO:0005634; C:nucleus; HDA:PomBase. DR GO; GO:0005840; C:ribosome; IBA:GO_Central. DR GO; GO:0003735; F:structural constituent of ribosome; IBA:GO_Central. DR GO; GO:0002181; P:cytoplasmic translation; ISO:PomBase. DR GO; GO:2000640; P:positive regulation of SREBP signaling pathway; IMP:PomBase. DR GO; GO:0006412; P:translation; IBA:GO_Central. DR CDD; cd03367; Ribosomal_S23; 1. DR Gene3D; 2.40.50.140; Nucleic acid-binding proteins; 1. DR InterPro; IPR012340; NA-bd_OB-fold. DR InterPro; IPR006032; Ribosomal_uS12. DR InterPro; IPR005680; Ribosomal_uS12_euk/arc. DR NCBIfam; TIGR00982; uS12_E_A; 1. DR PANTHER; PTHR11652; 30S RIBOSOMAL PROTEIN S12 FAMILY MEMBER; 1. DR PANTHER; PTHR11652:SF14; 40S RIBOSOMAL PROTEIN S23; 1. DR Pfam; PF00164; Ribosom_S12_S23; 1. DR PIRSF; PIRSF002133; Ribosomal_S12/S23; 1. DR SUPFAM; SSF50249; Nucleic acid-binding proteins; 1. DR PROSITE; PS00055; RIBOSOMAL_S12; 1. PE 1: Evidence at protein level; KW Cytoplasm; Hydroxylation; Nucleus; Reference proteome; Ribonucleoprotein; KW Ribosomal protein. FT CHAIN 1..143 FT /note="Small ribosomal subunit protein uS12A" FT /id="PRO_0000146479" FT MOD_RES 62 FT /note="3,4-dihydroxyproline" FT /evidence="ECO:0000269|PubMed:24550462" SQ SEQUENCE 143 AA; 15655 MW; 8511D5838F472D53 CRC64; MGKPAGLNAA RKLRNHRREE RWADAHYKKR LLGTAYKSSP FGGSSHAKGI VVEKIGVEAK QPNSAIRKCV RVQLIKNGKK VTAFVPHDGC LNFVDENDEV LLSGFGRKGK AKGDIPGVRF KVVKVAGVGL SALFHEKKEK PRA //