ID   ENASE_CHICK             Reviewed;         728 AA.
AC   P0C7A1;
DT   08-APR-2008, integrated into UniProtKB/Swiss-Prot.
DT   08-APR-2008, sequence version 1.
DT   05-DEC-2018, entry version 61.
DE   RecName: Full=Cytosolic endo-beta-N-acetylglucosaminidase;
DE            Short=ENGase;
DE            EC=3.2.1.96;
GN   Name=ENGASE;
OS   Gallus gallus (Chicken).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC   Archelosauria; Archosauria; Dinosauria; Saurischia; Theropoda;
OC   Coelurosauria; Aves; Neognathae; Galloanserae; Galliformes;
OC   Phasianidae; Phasianinae; Gallus.
OX   NCBI_TaxID=9031;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=Red jungle fowl;
RX   PubMed=15592404; DOI=10.1038/nature03154;
RA   Hillier L.W., Miller W., Birney E., Warren W., Hardison R.C.,
RA   Ponting C.P., Bork P., Burt D.W., Groenen M.A.M., Delany M.E.,
RA   Dodgson J.B., Chinwalla A.T., Cliften P.F., Clifton S.W.,
RA   Delehaunty K.D., Fronick C., Fulton R.S., Graves T.A., Kremitzki C.,
RA   Layman D., Magrini V., McPherson J.D., Miner T.L., Minx P., Nash W.E.,
RA   Nhan M.N., Nelson J.O., Oddy L.G., Pohl C.S., Randall-Maher J.,
RA   Smith S.M., Wallis J.W., Yang S.-P., Romanov M.N., Rondelli C.M.,
RA   Paton B., Smith J., Morrice D., Daniels L., Tempest H.G.,
RA   Robertson L., Masabanda J.S., Griffin D.K., Vignal A., Fillon V.,
RA   Jacobbson L., Kerje S., Andersson L., Crooijmans R.P., Aerts J.,
RA   van der Poel J.J., Ellegren H., Caldwell R.B., Hubbard S.J.,
RA   Grafham D.V., Kierzek A.M., McLaren S.R., Overton I.M., Arakawa H.,
RA   Beattie K.J., Bezzubov Y., Boardman P.E., Bonfield J.K.,
RA   Croning M.D.R., Davies R.M., Francis M.D., Humphray S.J., Scott C.E.,
RA   Taylor R.G., Tickle C., Brown W.R.A., Rogers J., Buerstedde J.-M.,
RA   Wilson S.A., Stubbs L., Ovcharenko I., Gordon L., Lucas S.,
RA   Miller M.M., Inoko H., Shiina T., Kaufman J., Salomonsen J.,
RA   Skjoedt K., Wong G.K.-S., Wang J., Liu B., Wang J., Yu J., Yang H.,
RA   Nefedov M., Koriabine M., Dejong P.J., Goodstadt L., Webber C.,
RA   Dickens N.J., Letunic I., Suyama M., Torrents D., von Mering C.,
RA   Zdobnov E.M., Makova K., Nekrutenko A., Elnitski L., Eswara P.,
RA   King D.C., Yang S.-P., Tyekucheva S., Radakrishnan A., Harris R.S.,
RA   Chiaromonte F., Taylor J., He J., Rijnkels M., Griffiths-Jones S.,
RA   Ureta-Vidal A., Hoffman M.M., Severin J., Searle S.M.J., Law A.S.,
RA   Speed D., Waddington D., Cheng Z., Tuzun E., Eichler E., Bao Z.,
RA   Flicek P., Shteynberg D.D., Brent M.R., Bye J.M., Huckle E.J.,
RA   Chatterji S., Dewey C., Pachter L., Kouranov A., Mourelatos Z.,
RA   Hatzigeorgiou A.G., Paterson A.H., Ivarie R., Brandstrom M.,
RA   Axelsson E., Backstrom N., Berlin S., Webster M.T., Pourquie O.,
RA   Reymond A., Ucla C., Antonarakis S.E., Long M., Emerson J.J.,
RA   Betran E., Dupanloup I., Kaessmann H., Hinrichs A.S., Bejerano G.,
RA   Furey T.S., Harte R.A., Raney B., Siepel A., Kent W.J., Haussler D.,
RA   Eyras E., Castelo R., Abril J.F., Castellano S., Camara F., Parra G.,
RA   Guigo R., Bourque G., Tesler G., Pevzner P.A., Smit A., Fulton L.A.,
RA   Mardis E.R., Wilson R.K.;
RT   "Sequence and comparative analysis of the chicken genome provide
RT   unique perspectives on vertebrate evolution.";
RL   Nature 432:695-716(2004).
RN   [2]
RP   PROTEIN SEQUENCE OF 51-76, AND SUBCELLULAR LOCATION.
RX   PubMed=12114544; DOI=10.1073/pnas.152333599;
RA   Suzuki T., Yano K., Sugimoto S., Kitajima K., Lennarz W.J., Inoue S.,
RA   Inoue Y., Emori Y.;
RT   "Endo-beta-N-acetylglucosaminidase, an enzyme involved in processing
RT   of free oligosaccharides in the cytosol.";
RL   Proc. Natl. Acad. Sci. U.S.A. 99:9691-9696(2002).
CC   -!- FUNCTION: Endoglycosidase that releases N-glycans from
CC       glycoproteins by cleaving the beta-1,4-glycosidic bond in the
CC       N,N'-diacetylchitobiose core. Involved in the processing of free
CC       oligosaccharides in the cytosol.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=Endohydrolysis of the N,N'-diacetylchitobiosyl unit in
CC         high-mannose glycopeptides and glycoproteins containing the
CC         -(Man(GlcNAc)(2))Asn-structure. One N-acetyl-D-glucosamine
CC         residue remains attached to the protein, the rest of the
CC         oligosaccharide is released intact.; EC=3.2.1.96;
CC   -!- SUBCELLULAR LOCATION: Cytoplasm, cytosol
CC       {ECO:0000305|PubMed:12114544}.
CC   -!- SIMILARITY: Belongs to the glycosyl hydrolase 85 family.
CC       {ECO:0000305}.
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DR   ProteinModelPortal; P0C7A1; -.
DR   STRING; 9031.ENSGALP00000019265; -.
DR   ChEMBL; CHEMBL1075083; -.
DR   PaxDb; P0C7A1; -.
DR   PRIDE; P0C7A1; -.
DR   eggNOG; KOG2331; Eukaryota.
DR   eggNOG; COG4724; LUCA.
DR   HOGENOM; HOG000082678; -.
DR   HOVERGEN; HBG107848; -.
DR   InParanoid; P0C7A1; -.
DR   PhylomeDB; P0C7A1; -.
DR   Proteomes; UP000000539; Unplaced.
DR   GO; GO:0005829; C:cytosol; IEA:UniProtKB-SubCell.
DR   GO; GO:0005615; C:extracellular space; IDA:AgBase.
DR   GO; GO:0005764; C:lysosome; TAS:AgBase.
DR   GO; GO:0016231; F:beta-N-acetylglucosaminidase activity; IDA:AgBase.
DR   GO; GO:0016787; F:hydrolase activity; TAS:AgBase.
DR   GO; GO:0033925; F:mannosyl-glycoprotein endo-beta-N-acetylglucosaminidase activity; IEA:UniProtKB-EC.
DR   GO; GO:0051672; P:catabolism by organism of cell wall peptidoglycan in other organism; TAS:AgBase.
DR   GO; GO:0006517; P:protein deglycosylation; IBA:GO_Central.
DR   Gene3D; 2.60.120.260; -; 1.
DR   Gene3D; 2.60.120.40; -; 1.
DR   InterPro; IPR001073; C1q_dom.
DR   InterPro; IPR032979; ENGase.
DR   InterPro; IPR008979; Galactose-bd-like_sf.
DR   InterPro; IPR005201; Glyco_hydro_85.
DR   InterPro; IPR008983; Tumour_necrosis_fac-like_dom.
DR   PANTHER; PTHR13246; PTHR13246; 1.
DR   Pfam; PF00386; C1q; 1.
DR   Pfam; PF03644; Glyco_hydro_85; 1.
DR   SUPFAM; SSF49842; SSF49842; 1.
DR   PROSITE; PS50871; C1Q; 1.
PE   1: Evidence at protein level;
KW   Complete proteome; Cytoplasm; Direct protein sequencing; Glycosidase;
KW   Hydrolase; Reference proteome.
FT   CHAIN         1    728       Cytosolic endo-beta-N-
FT                                acetylglucosaminidase.
FT                                /FTId=PRO_0000328869.
FT   DOMAIN      287    381       BRCT.
SQ   SEQUENCE   728 AA;  80889 MW;  CAA311512C095918 CRC64;
     MQSQSVMLEL REQDEVWVRL YKGERENAVF SDEYDTYITF SGHLINFQPA AEPLGTTVLH
     AAVDTRPQPA RYFDTGTTEP VSFFLSGLEE LLAWHPSSDD EFNVCAVPLA QRQPPLHSRR
     PRTLLCHDMR GGYLEDRFIQ GSATRNPYVF YHWRYVDIFV YFSHHTVTIP PVCWTNAAHR
     NGVPVLGTFI TEWADGEKLC EAFLAGGEDA YRAVSHQLAR IAQHYRFDGW LINIENALSA
     AAVGNLSPFL RHLTAEVHGA VPGGLVIWYD SILESGTLRW QNELNQQNRV FFDACDGLFV
     NYNWKEEHLE RTRELAGQRH ADVYIGVDVF ARGDVVGGGF DTNKSLSLIR KHGLSAAIFA
     PGWVYKHLGE ENFLLNEDKF WGLLEDYLPT HSICTLPLAT SFSVGMGTGM FLAGKEEEAG
     PWYNLSAQEI QPLYPERRGW LSTSCCLQDA WCGGSSLRVQ GTIPPGEERV AIRLSLWVDL
     GSSGFSLSIC GTLASGPHRD DFTVALELTT WHSSRCHDGT VTVLPSEDEP HGRHHPHLLP
     APPPALSRML AACSHGAQGW TSRCYEQELR GCSLRDLSLL VSRQQASPQE TSFSCLLGEL
     RVLDAGSMAA SPPQVQSLTA SQLWWQDGPS AEQLSLSLTL RWAFPPGRAA CFRVLSQGAR
     CHRAQPAQPQ LLGLAHGCQY RAVGLAVPRP APGQSCQLEL LVEPVLPSEL PVGPERWGRL
     LLVYSEPA
//