ID ACTPP_ACTEQ Reviewed; 20 AA. AC P0C1H1; DT 27-JUN-2006, integrated into UniProtKB/Swiss-Prot. DT 27-JUN-2006, sequence version 1. DT 31-JUL-2019, entry version 37. DE RecName: Full=Equinatoxin-1'' {ECO:0000305}; DE AltName: Full=DELTA-actitoxin {ECO:0000305}; DE AltName: Full=Equinatoxin I'' {ECO:0000303|PubMed:10414864}; DE Flags: Fragment; OS Actinia equina (Beadlet anemone). OC Eukaryota; Metazoa; Cnidaria; Anthozoa; Hexacorallia; Actiniaria; OC Actiniidae; Actinia. OX NCBI_TaxID=6106; RN [1] RP PROTEIN SEQUENCE. RX PubMed=10414864; DOI=10.1016/S0041-0101(99)00082-3; RA Anderluh G., Krizaj I., Strukelj B., Gubensek F., Macek P., RA Pungercar J.; RT "Equinatoxins, pore-forming proteins from the sea anemone Actinia RT equina, belong to a multigene family."; RL Toxicon 37:1391-1401(1999). RN [2] RP AMINO-ACID COMPOSITION, AND LETHAL DOSE. RX PubMed=2903587; DOI=10.1016/0041-0101(88)90183-3; RA Macek P., Lebez D.; RT "Isolation and characterization of three lethal and hemolytic toxins RT from the sea anemone Actinia equina L."; RL Toxicon 26:441-451(1988). RN [3] RP REVIEW. RX PubMed=19268680; DOI=10.1016/j.toxicon.2009.02.026; RA Kristan K.C., Viero G., Dalla Serra M., Macek P., Anderluh G.; RT "Molecular mechanism of pore formation by actinoporins."; RL Toxicon 54:1125-1134(2009). CC -!- FUNCTION: Pore-forming protein that forms cations-selective CC hydrophilic pores of around 1 nm and causes cardiac stimulation CC and hemolysis. Pore formation is a multi-step process that CC involves specific recognition of membrane sphingomyelin (but CC neither cholesterol nor phosphatidylcholine) using aromatic rich CC region and adjacent phosphocholine (POC) binding site, firm CC binding to the membrane (mainly driven by hydrophobic CC interactions) accompanied by the transfer of the N-terminal region CC to the lipid-water interface and finally pore formation after CC oligomerization of monomers. Cytolytic effects include red blood CC cells hemolysis, platelet aggregation and lysis, cytotoxic and CC cytostatic effects on fibroblasts. Lethality in mammals has been CC ascribed to severe vasospasm of coronary vessels, cardiac CC arrhythmia, and inotropic effects. {ECO:0000250}. CC -!- SUBUNIT: Tetramer in the presence of a lipidic interface. Monomer, CC in soluble state. {ECO:0000250}. CC -!- SUBCELLULAR LOCATION: Secreted {ECO:0000305}. Nematocyst CC {ECO:0000305}. Target cell membrane {ECO:0000250}. Note=Forms an CC alpha-helical membrane channel in the prey. {ECO:0000250}. CC -!- DOMAIN: The N-terminal region, before the pore is formed, is bound CC to the lipid membrane. It partitions into the lipid-water CC interface and stabilizes the monomeric molecule on the membrane. CC Finally, it traverses the bilayer, thus forming the transmembrane CC pore. {ECO:0000250|UniProtKB:P61914}. CC -!- TOXIC DOSE: LD(50) is 23 ug/kg by intravenous injection into mice. CC {ECO:0000269|PubMed:2903587}. CC -!- SIMILARITY: Belongs to the actinoporin family. Sea anemone CC subfamily. {ECO:0000305}. CC --------------------------------------------------------------------------- CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms CC Distributed under the Creative Commons Attribution (CC BY 4.0) License CC --------------------------------------------------------------------------- DR GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell. DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW. DR GO; GO:0042151; C:nematocyst; IEA:UniProtKB-SubCell. DR GO; GO:0044218; C:other organism cell membrane; IEA:UniProtKB-SubCell. DR GO; GO:0090729; F:toxin activity; IEA:UniProtKB-KW. DR GO; GO:0044179; P:hemolysis in other organism; IEA:UniProtKB-KW. DR GO; GO:0006811; P:ion transport; IEA:UniProtKB-KW. PE 1: Evidence at protein level; KW Cytolysis; Direct protein sequencing; Hemolysis; Ion transport; KW Membrane; Nematocyst; Secreted; Target cell membrane; Target membrane; KW Toxin; Transmembrane; Transport. FT PEPTIDE 1 >20 Equinatoxin-1''. FT /FTId=PRO_0000244624. FT REGION 3 12 Plays an important role in the hemolytic FT activity. {ECO:0000250}. FT REGION 11 >20 N-terminal region. {ECO:0000250}. FT NON_TER 20 20 SQ SEQUENCE 20 AA; 1960 MW; FA32B426009FF5FA CRC64; SVAVAGAVIE GATLTFNVLQ //