ID NAGC_ECOLI Reviewed; 406 AA. AC P0AF20; P15301; DT 20-DEC-2005, integrated into UniProtKB/Swiss-Prot. DT 20-DEC-2005, sequence version 1. DT 22-FEB-2023, entry version 116. DE RecName: Full=N-acetylglucosamine repressor; GN Name=nagC; Synonyms=nagR; OrderedLocusNames=b0676, JW0662; OS Escherichia coli (strain K12). OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales; OC Enterobacteriaceae; Escherichia. OX NCBI_TaxID=83333; RN [1] RP NUCLEOTIDE SEQUENCE [GENOMIC DNA]. RC STRAIN=K12; RX PubMed=2668691; DOI=10.1111/j.1365-2958.1989.tb00197.x; RA Plumbridge J.; RT "Sequence of the nagBACD operon in Escherichia coli K12 and pattern of RT transcription within the nag regulon."; RL Mol. Microbiol. 3:505-515(1989). RN [2] RP NUCLEOTIDE SEQUENCE [GENOMIC DNA]. RC STRAIN=K12; RX PubMed=2190615; DOI=10.1139/o90-017; RA Peri K.G., Goldie H., Waygood E.B.; RT "Cloning and characterization of the N-acetylglucosamine operon of RT Escherichia coli."; RL Biochem. Cell Biol. 68:123-137(1990). RN [3] RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. RC STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911; RX PubMed=8905232; DOI=10.1093/dnares/3.3.137; RA Oshima T., Aiba H., Baba T., Fujita K., Hayashi K., Honjo A., Ikemoto K., RA Inada T., Itoh T., Kajihara M., Kanai K., Kashimoto K., Kimura S., RA Kitagawa M., Makino K., Masuda S., Miki T., Mizobuchi K., Mori H., RA Motomura K., Nakamura Y., Nashimoto H., Nishio Y., Saito N., Sampei G., RA Seki Y., Tagami H., Takemoto K., Wada C., Yamamoto Y., Yano M., RA Horiuchi T.; RT "A 718-kb DNA sequence of the Escherichia coli K-12 genome corresponding to RT the 12.7-28.0 min region on the linkage map."; RL DNA Res. 3:137-155(1996). RN [4] RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. RC STRAIN=K12 / MG1655 / ATCC 47076; RX PubMed=9278503; DOI=10.1126/science.277.5331.1453; RA Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., RA Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., RA Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., RA Shao Y.; RT "The complete genome sequence of Escherichia coli K-12."; RL Science 277:1453-1462(1997). RN [5] RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. RC STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911; RX PubMed=16738553; DOI=10.1038/msb4100049; RA Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., RA Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.; RT "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 RT and W3110."; RL Mol. Syst. Biol. 2:E1-E5(2006). RN [6] RP FUNCTION. RX PubMed=7545108; DOI=10.1002/j.1460-2075.1995.tb00067.x; RA Plumbridge J.; RT "Co-ordinated regulation of amino sugar biosynthesis and degradation: the RT NagC repressor acts as both an activator and a repressor for the RT transcription of the glmUS operon and requires two separated NagC binding RT sites."; RL EMBO J. 14:3958-3965(1995). CC -!- FUNCTION: Acts as a repressor of the nagEBACD operon and acts both as CC an activator and a repressor for the transcription of the glmSU operon. CC {ECO:0000269|PubMed:7545108}. CC -!- INDUCTION: By N-acetylglucosamine. CC -!- SIMILARITY: Belongs to the ROK (NagC/XylR) family. {ECO:0000305}. CC --------------------------------------------------------------------------- CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms CC Distributed under the Creative Commons Attribution (CC BY 4.0) License CC --------------------------------------------------------------------------- DR EMBL; X14135; CAA32354.1; -; Genomic_DNA. DR EMBL; AF052007; AAC09326.1; -; Genomic_DNA. DR EMBL; U00096; AAC73770.1; -; Genomic_DNA. DR EMBL; AP009048; BAA35319.1; -; Genomic_DNA. DR PIR; C64802; C64802. DR RefSeq; NP_415202.1; NC_000913.3. DR RefSeq; WP_000187594.1; NZ_STEB01000044.1. DR AlphaFoldDB; P0AF20; -. DR SMR; P0AF20; -. DR BioGRID; 4261210; 133. DR DIP; DIP-35984N; -. DR IntAct; P0AF20; 9. DR STRING; 511145.b0676; -. DR jPOST; P0AF20; -. DR PaxDb; P0AF20; -. DR EnsemblBacteria; AAC73770; AAC73770; b0676. DR EnsemblBacteria; BAA35319; BAA35319; BAA35319. DR GeneID; 66671054; -. DR GeneID; 945285; -. DR KEGG; ecj:JW0662; -. DR KEGG; eco:b0676; -. DR PATRIC; fig|1411691.4.peg.1602; -. DR EchoBASE; EB0630; -. DR eggNOG; COG1846; Bacteria. DR eggNOG; COG1940; Bacteria. DR HOGENOM; CLU_036604_13_1_6; -. DR InParanoid; P0AF20; -. DR OMA; VDFGHTH; -. DR OrthoDB; 3189808at2; -. DR PhylomeDB; P0AF20; -. DR BioCyc; EcoCyc:PD00266; -. DR PRO; PR:P0AF20; -. DR Proteomes; UP000000318; Chromosome. DR Proteomes; UP000000625; Chromosome. DR GO; GO:0003677; F:DNA binding; IDA:EcoCyc. DR GO; GO:0003700; F:DNA-binding transcription factor activity; IEA:InterPro. DR GO; GO:0005975; P:carbohydrate metabolic process; IEA:UniProtKB-KW. DR GO; GO:0006355; P:regulation of DNA-templated transcription; IMP:EcoCyc. DR Gene3D; 3.30.420.40; -; 2. DR Gene3D; 1.10.10.10; Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain; 1. DR InterPro; IPR043129; ATPase_NBD. DR InterPro; IPR000835; HTH_MarR-typ. DR InterPro; IPR000600; ROK. DR InterPro; IPR036388; WH-like_DNA-bd_sf. DR InterPro; IPR036390; WH_DNA-bd_sf. DR PANTHER; PTHR18964:SF175; N-ACETYLGLUCOSAMINE REPRESSOR; 1. DR PANTHER; PTHR18964; ROK (REPRESSOR, ORF, KINASE) FAMILY; 1. DR Pfam; PF01047; MarR; 1. DR Pfam; PF00480; ROK; 1. DR SUPFAM; SSF53067; Actin-like ATPase domain; 1. DR SUPFAM; SSF46785; Winged helix' DNA-binding domain; 1. DR PROSITE; PS01125; ROK; 1. PE 2: Evidence at transcript level; KW Activator; Carbohydrate metabolism; DNA-binding; Reference proteome; KW Repressor; Transcription; Transcription regulation. FT CHAIN 1..406 FT /note="N-acetylglucosamine repressor" FT /id="PRO_0000095691" FT DNA_BIND 35..44 FT /note="H-T-H motif" FT /evidence="ECO:0000250" FT CONFLICT 203..204 FT /note="EH -> DD (in Ref. 1; CAA32354)" FT /evidence="ECO:0000305" SQ SEQUENCE 406 AA; 44541 MW; 28C4C85D736627C8 CRC64; MTPGGQAQIG NVDLVKQLNS AAVYRLIDQY GPISRIQIAE QSQLAPASVT KITRQLIERG LIKEVDQQAS TGGRRAISIV TETRNFHAIG VRLGRHDATI TLFDLSSKVL AEEHYPLPER TQQTLEHALL NAIAQFIDSY QRKLRELIAI SVILPGLVDP DSGKIHYMPH IQVENWGLVE ALEERFKVTC FVGHDIRSLA LAEHYFGASQ DCEDSILVRV HRGTGAGIIS NGRIFIGRNG NVGEIGHIQV EPLGERCHCG NFGCLETIAA NAAIEQRVLN LLKQGYQSRV PLDDCTIKTI CKAANKGDSL ASEVIEYVGR HLGKTIAIAI NLFNPQKIVI AGEITEADKV LLPAIESCIN TQALKAFRTN LPVVRSELDH RSAIGAFALV KRAMLNGILL QHLLEN //