ID IVY_ECOLI STANDARD; PRT; 157 AA. AC P0AD59; P45502; P77185; DT 06-DEC-2005, integrated into UniProtKB/Swiss-Prot. DT 06-DEC-2005, sequence version 1. DT 07-MAR-2006, entry version 4. DE Inhibitor of vertebrate lysozyme precursor. GN Name=ivy; OrderedLocusNames=b0220; OS Escherichia coli. OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacteriales; OC Enterobacteriaceae; Escherichia. OX NCBI_TaxID=562; RN [1] RP NUCLEOTIDE SEQUENCE [GENOMIC DNA]. RC STRAIN=K12 / W3110; RA Takemoto K., Mori H., Murayama N., Kataoka K., Yano M., Itoh T., RA Yamamoto Y., Inokuchi H., Miki T., Hatada E., Fukuda R., Ichihara S., RA Mizuno T., Makino K., Nakata A., Yura T., Sampei G., Mizobuchi K.; RT "Systematic sequencing of the Escherichia coli genome: analysis of the RT 4.0 - 6.0 min (189,987 - 281,416bp) region."; RL Submitted (FEB-1996) to the EMBL/GenBank/DDBJ databases. RN [2] RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. RC STRAIN=K12 / MG1655; RA Chung E., Allen E., Araujo R., Aparicio A.M., Davis K., Duncan M., RA Federspiel N., Hyman R., Kalman S., Komp C., Kurdi O., Lew H., Lin D., RA Namath A., Oefner P., Roberts D., Schramm S., Davis R.W.; RT "Sequence of minutes 4-25 of Escherichia coli."; RL Submitted (JAN-1997) to the EMBL/GenBank/DDBJ databases. RN [3] RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. RC STRAIN=K12 / MG1655; RX MEDLINE=97426617; PubMed=9278503; DOI=10.1126/science.277.5331.1453; RA Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., RA Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., RA Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., RA Mau B., Shao Y.; RT "The complete genome sequence of Escherichia coli K-12."; RL Science 277:1453-1474(1997). RN [4] RP PROTEIN SEQUENCE OF 29-39. RC STRAIN=K12 / W3110; RA Pasquali C., Sanchez J.-C., Ravier F., Golaz O., Hughes G.J., RA Frutiger S., Paquet N., Wilkins M., Appel R.D., Bairoch A., RA Hochstrasser D.F.; RL Submitted (AUG-1995) to Swiss-Prot. RN [5] RP PROTEIN SEQUENCE OF 29-38. RC STRAIN=K12; RX MEDLINE=99085675; PubMed=9868784; RA Wasinger V.C., Humphery-Smith I.; RT "Small genes/gene-products in Escherichia coli K-12."; RL FEMS Microbiol. Lett. 169:375-382(1998). RN [6] RP CHARACTERIZATION. RC STRAIN=K12 / XL1-Blue; RX MEDLINE=21264642; PubMed=11278658; DOI=10.1074/jbc.M010297200; RA Montchois V., Abergel C., Sturgis J., Jeudy S., Claverie J.-M.; RT "Escherichia coli ykfE ORFan gene encodes a potent inhibitor of C-type RT lysozyme."; RL J. Biol. Chem. 276:18437-18441(2001). RN [7] RP CRYSTALLIZATION. RX MEDLINE=20545966; PubMed=11092949; DOI=10.1107/S0907444900015316; RA Abergel C., Monchois V., Chenivesse S., Jeudy S., Claverie J.-M.; RT "Crystallization and preliminary crystallographic study of b0220, an RT 'ORFan' protein of unknown function from Escherichia coli."; RL Acta Crystallogr. D 56:1694-1695(2000). RN [8] RP X-RAY CRYSTALLOGRAPHY (1.6 ANGSTROMS). RA Abergel C., Monchois V., Claverie J.-M.; RT "Structure and evolution of a paradoxical protein family of vertebrate RT lysozyme inhibitors only found in Gram-negative bacteria."; RL Submitted (NOV-2001) to the PDB data bank. CC -!- FUNCTION: Strong inhibitor of lysozyme C. CC -!- SUBUNIT: Homodimer. CC -!- SUBCELLULAR LOCATION: Periplasmic. CC -!- SIMILARITY: Belongs to the ivy family. CC --------------------------------------------------------------------------- CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms CC Distributed under the Creative Commons Attribution (CC BY 4.0) License CC --------------------------------------------------------------------------- DR EMBL; D83536; BAA77890.1; -; Genomic_DNA. DR EMBL; U70214; AAB08642.1; -; Genomic_DNA. DR EMBL; U00096; AAC73324.1; -; Genomic_DNA. DR PIR; E64746; E64746. DR PDB; 1GPQ; X-ray; A/B=29-156. DR PDB; 1XS0; X-ray; A/B/C=29-156. DR SWISS-2DPAGE; P0AD59; COLI. DR GenomeReviews; U00096_GR; b0220. DR EchoBASE; EB3317; -. DR EcoGene; EG13547; ivy. DR BioCyc; EcoCyc:G6104-MONOMER; -. KW 3D-structure; Complete proteome; Direct protein sequencing; KW Periplasmic; Signal. FT SIGNAL 1 28 FT CHAIN 29 157 Inhibitor of vertebrate lysozyme. FT /FTId=PRO_0000016546. FT SITE 88 88 Important for lysozyme inhibition. FT DISULFID 85 90 FT CONFLICT 36 36 Missing (in Ref. 5). SQ SEQUENCE 157 AA; 16872 MW; 9156C5944DFE6D84 CRC64; MGRISSGGMM FKAITTVAAL VIATSAMAQD DLTISSLAKG ETTKAAFNQM VQGHKLPAWV MKGGTYTPAQ TVTLGDETYQ VMSACKPHDC GSQRIAVMWS EKSNQMTGLF STIDEKTSQE KLTWLNVNDA LSIDGKTVLF AALTGSLENH PDGFNFK //