ID IVY_ECOLI Reviewed; 157 AA. AC P0AD59; P45502; P77185; DT 06-DEC-2005, integrated into UniProtKB/Swiss-Prot. DT 06-DEC-2005, sequence version 1. DT 24-NOV-2009, entry version 39. DE RecName: Full=Inhibitor of vertebrate lysozyme; DE Flags: Precursor; GN Name=ivy; Synonyms=ykfE; OrderedLocusNames=b0220, JW0210; OS Escherichia coli (strain K12). OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacteriales; OC Enterobacteriaceae; Escherichia. OX NCBI_TaxID=83333; RN [1] RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. RC STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911; RA Takemoto K., Mori H., Murayama N., Kataoka K., Yano M., Itoh T., RA Yamamoto Y., Inokuchi H., Miki T., Hatada E., Fukuda R., Ichihara S., RA Mizuno T., Makino K., Nakata A., Yura T., Sampei G., Mizobuchi K.; RT "Systematic sequencing of the Escherichia coli genome: analysis of the RT 4.0 - 6.0 min (189,987 - 281,416bp) region."; RL Submitted (FEB-1996) to the EMBL/GenBank/DDBJ databases. RN [2] RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. RC STRAIN=K12 / MG1655 / ATCC 47076; RA Chung E., Allen E., Araujo R., Aparicio A.M., Davis K., Duncan M., RA Federspiel N., Hyman R., Kalman S., Komp C., Kurdi O., Lew H., Lin D., RA Namath A., Oefner P., Roberts D., Schramm S., Davis R.W.; RT "Sequence of minutes 4-25 of Escherichia coli."; RL Submitted (JAN-1997) to the EMBL/GenBank/DDBJ databases. RN [3] RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. RC STRAIN=K12 / MG1655 / ATCC 47076; RX MEDLINE=97426617; PubMed=9278503; DOI=10.1126/science.277.5331.1453; RA Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., RA Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., RA Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., RA Mau B., Shao Y.; RT "The complete genome sequence of Escherichia coli K-12."; RL Science 277:1453-1474(1997). RN [4] RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. RC STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911; RX PubMed=16738553; DOI=10.1038/msb4100049; RA Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., RA Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.; RT "Highly accurate genome sequences of Escherichia coli K-12 strains RT MG1655 and W3110."; RL Mol. Syst. Biol. 2:E1-E5(2006). RN [5] RP PROTEIN SEQUENCE OF 29-39. RC STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911; RA Pasquali C., Sanchez J.-C., Ravier F., Golaz O., Hughes G.J., RA Frutiger S., Paquet N., Wilkins M., Appel R.D., Bairoch A., RA Hochstrasser D.F.; RL Submitted (AUG-1995) to UniProtKB. RN [6] RP PROTEIN SEQUENCE OF 29-38. RC STRAIN=K12; RX MEDLINE=99085675; PubMed=9868784; RX DOI=10.1111/j.1574-6968.1998.tb13343.x; RA Wasinger V.C., Humphery-Smith I.; RT "Small genes/gene-products in Escherichia coli K-12."; RL FEMS Microbiol. Lett. 169:375-382(1998). RN [7] RP CHARACTERIZATION. RC STRAIN=K12 / XL1-Blue; RX MEDLINE=21264642; PubMed=11278658; DOI=10.1074/jbc.M010297200; RA Montchois V., Abergel C., Sturgis J., Jeudy S., Claverie J.-M.; RT "Escherichia coli ykfE ORFan gene encodes a potent inhibitor of C-type RT lysozyme."; RL J. Biol. Chem. 276:18437-18441(2001). RN [8] RP CRYSTALLIZATION. RX MEDLINE=20545966; PubMed=11092949; DOI=10.1107/S0907444900015316; RA Abergel C., Monchois V., Chenivesse S., Jeudy S., Claverie J.-M.; RT "Crystallization and preliminary crystallographic study of b0220, an RT 'ORFan' protein of unknown function from Escherichia coli."; RL Acta Crystallogr. D 56:1694-1695(2000). RN [9] RP X-RAY CRYSTALLOGRAPHY (1.6 ANGSTROMS). RA Abergel C., Monchois V., Claverie J.-M.; RT "Structure and evolution of a paradoxical protein family of vertebrate RT lysozyme inhibitors only found in Gram-negative bacteria."; RL Submitted (NOV-2001) to the PDB data bank. CC -!- FUNCTION: Strong inhibitor of lysozyme C. CC -!- SUBUNIT: Homodimer. CC -!- SUBCELLULAR LOCATION: Periplasm. CC -!- SIMILARITY: Belongs to the ivy family. CC --------------------------------------------------------------------------- CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms CC Distributed under the Creative Commons Attribution (CC BY 4.0) License CC --------------------------------------------------------------------------- DR EMBL; U70214; AAB08642.1; -; Genomic_DNA. DR EMBL; U00096; AAC73324.1; -; Genomic_DNA. DR EMBL; AP009048; BAA77890.1; -; Genomic_DNA. DR PIR; E64746; E64746. DR RefSeq; AP_000875.1; -. DR RefSeq; NP_414755.1; -. DR PDB; 1GPQ; X-ray; 1.60 A; A/B=29-156. DR PDB; 1XS0; X-ray; 1.58 A; A/B/C=29-156. DR PDBsum; 1GPQ; -. DR PDBsum; 1XS0; -. DR STRING; P0AD59; -. DR SWISS-2DPAGE; P0AD59; -. DR GeneID; 946530; -. DR GenomeReviews; AP009048_GR; JW0210. DR GenomeReviews; U00096_GR; b0220. DR KEGG; ecj:JW0210; -. DR KEGG; eco:b0220; -. DR EchoBASE; EB3317; -. DR EcoGene; EG13547; ivy. DR HOGENOM; P0AD59; -. DR OMA; NHPDAFN; -. DR BioCyc; EcoCyc:G6104-MON; -. DR BioCyc; ECOL168927:B0220-MON; -. DR Genevestigator; P0AD59; -. DR GO; GO:0042597; C:periplasmic space; IEA:UniProtKB-SubCell. DR GO; GO:0043086; P:negative regulation of catalytic activity; IEA:InterPro. DR InterPro; IPR014453; Ihibitor_vertebrate_lysozyme. DR Gene3D; G3DSA:3.40.1420.10; Ihibitor_vertebrate_lysozyme; 1. DR Pfam; PF08816; Ivy; 1. DR PIRSF; PIRSF009103; Ivy; 1. PE 1: Evidence at protein level; KW 3D-structure; Complete proteome; Direct protein sequencing; KW Disulfide bond; Periplasm; Signal. FT SIGNAL 1 28 FT CHAIN 29 157 Inhibitor of vertebrate lysozyme. FT /FTId=PRO_0000016546. FT SITE 88 88 Important for lysozyme inhibition. FT DISULFID 85 90 FT CONFLICT 36 36 Missing (in Ref. 6; AA sequence). FT HELIX 34 39 FT TURN 41 43 FT HELIX 44 51 FT HELIX 59 62 FT STRAND 70 74 FT STRAND 77 85 FT TURN 90 92 FT STRAND 93 99 FT TURN 101 103 FT STRAND 106 114 FT HELIX 115 117 FT STRAND 119 126 FT HELIX 129 131 FT HELIX 134 144 FT HELIX 146 149 SQ SEQUENCE 157 AA; 16872 MW; 9156C5944DFE6D84 CRC64; MGRISSGGMM FKAITTVAAL VIATSAMAQD DLTISSLAKG ETTKAAFNQM VQGHKLPAWV MKGGTYTPAQ TVTLGDETYQ VMSACKPHDC GSQRIAVMWS EKSNQMTGLF STIDEKTSQE KLTWLNVNDA LSIDGKTVLF AALTGSLENH PDGFNFK //