ID   HYBE_ECOLI              Reviewed;         162 AA.
AC   P0AAN1; P37183; Q2M9K3;
DT   11-OCT-2005, integrated into UniProtKB/Swiss-Prot.
DT   11-OCT-2005, sequence version 1.
DT   08-NOV-2023, entry version 111.
DE   RecName: Full=Hydrogenase-2 operon protein HybE;
GN   Name=hybE; OrderedLocusNames=b2992, JW2960;
OS   Escherichia coli (strain K12).
OC   Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
OC   Enterobacteriaceae; Escherichia.
OX   NCBI_TaxID=83333;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=K12 / TG1;
RX   PubMed=8021226; DOI=10.1128/jb.176.14.4416-4423.1994;
RA   Menon N.K., Chatelus C.Y., Dervartanian M., Wendt J.C., Shanmugam K.T.,
RA   Peck H.D. Jr., Przybyla A.E.;
RT   "Cloning, sequencing, and mutational analysis of the hyb operon encoding
RT   Escherichia coli hydrogenase 2.";
RL   J. Bacteriol. 176:4416-4423(1994).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=K12 / MG1655 / ATCC 47076;
RX   PubMed=9278503; DOI=10.1126/science.277.5331.1453;
RA   Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V.,
RA   Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F.,
RA   Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B.,
RA   Shao Y.;
RT   "The complete genome sequence of Escherichia coli K-12.";
RL   Science 277:1453-1462(1997).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX   PubMed=16738553; DOI=10.1038/msb4100049;
RA   Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S.,
RA   Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.;
RT   "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655
RT   and W3110.";
RL   Mol. Syst. Biol. 2:E1-E5(2006).
CC   -!- INTERACTION:
CC       P0AAN1; P69741: hybO; NbExp=6; IntAct=EBI-552588, EBI-552619;
CC   -!- SIMILARITY: Belongs to the HupJ family. {ECO:0000305}.
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DR   EMBL; U09177; AAA21593.1; -; Genomic_DNA.
DR   EMBL; U28377; AAA69159.1; -; Genomic_DNA.
DR   EMBL; U00096; AAC76028.1; -; Genomic_DNA.
DR   EMBL; AP009048; BAE77053.1; -; Genomic_DNA.
DR   PIR; F65085; F65085.
DR   RefSeq; NP_417466.1; NC_000913.3.
DR   RefSeq; WP_000134014.1; NZ_STEB01000001.1.
DR   PDB; 2KC5; NMR; -; A=1-162.
DR   PDBsum; 2KC5; -.
DR   AlphaFoldDB; P0AAN1; -.
DR   BMRB; P0AAN1; -.
DR   SMR; P0AAN1; -.
DR   BioGRID; 4262373; 13.
DR   DIP; DIP-35795N; -.
DR   IntAct; P0AAN1; 7.
DR   MINT; P0AAN1; -.
DR   STRING; 511145.b2992; -.
DR   jPOST; P0AAN1; -.
DR   PaxDb; 511145-b2992; -.
DR   EnsemblBacteria; AAC76028; AAC76028; b2992.
DR   EnsemblBacteria; BAE77053; BAE77053; BAE77053.
DR   GeneID; 75173125; -.
DR   GeneID; 947483; -.
DR   KEGG; ecj:JW2960; -.
DR   KEGG; eco:b2992; -.
DR   PATRIC; fig|1411691.4.peg.3737; -.
DR   EchoBASE; EB1751; -.
DR   eggNOG; COG1773; Bacteria.
DR   HOGENOM; CLU_091699_2_0_6; -.
DR   InParanoid; P0AAN1; -.
DR   OMA; LTPWMIS; -.
DR   OrthoDB; 6485044at2; -.
DR   PhylomeDB; P0AAN1; -.
DR   BioCyc; EcoCyc:EG11803-MONOMER; -.
DR   EvolutionaryTrace; P0AAN1; -.
DR   PRO; PR:P0AAN1; -.
DR   Proteomes; UP000000318; Chromosome.
DR   Proteomes; UP000000625; Chromosome.
DR   GO; GO:0070678; F:preprotein binding; IPI:EcoCyc.
DR   GO; GO:0051604; P:protein maturation; IMP:EcoCyc.
DR   Gene3D; 3.30.1460.40; [NiFe]-hydrogenase assembly chaperone, HybE; 1.
DR   InterPro; IPR023994; NiFe-hyd_HybE.
DR   InterPro; IPR038530; NiFe-hyd_HybE_sf.
DR   NCBIfam; TIGR03993; hydrog_HybE; 1.
DR   Pfam; PF11939; NiFe-hyd_HybE; 1.
PE   1: Evidence at protein level;
KW   3D-structure; Reference proteome.
FT   CHAIN           1..162
FT                   /note="Hydrogenase-2 operon protein HybE"
FT                   /id="PRO_0000201426"
FT   CONFLICT        7
FT                   /note="G -> A (in Ref. 1; AAA21593)"
FT                   /evidence="ECO:0000305"
FT   STRAND          4..6
FT                   /evidence="ECO:0007829|PDB:2KC5"
FT   HELIX           13..23
FT                   /evidence="ECO:0007829|PDB:2KC5"
FT   HELIX           29..31
FT                   /evidence="ECO:0007829|PDB:2KC5"
FT   STRAND          41..48
FT                   /evidence="ECO:0007829|PDB:2KC5"
FT   STRAND          51..57
FT                   /evidence="ECO:0007829|PDB:2KC5"
FT   STRAND          62..68
FT                   /evidence="ECO:0007829|PDB:2KC5"
FT   STRAND          73..75
FT                   /evidence="ECO:0007829|PDB:2KC5"
FT   STRAND          82..85
FT                   /evidence="ECO:0007829|PDB:2KC5"
FT   STRAND          92..99
FT                   /evidence="ECO:0007829|PDB:2KC5"
FT   TURN            100..102
FT                   /evidence="ECO:0007829|PDB:2KC5"
FT   STRAND          103..111
FT                   /evidence="ECO:0007829|PDB:2KC5"
FT   HELIX           120..135
FT                   /evidence="ECO:0007829|PDB:2KC5"
SQ   SEQUENCE   162 AA;  17964 MW;  CFDAEA3D47BE9516 CRC64;
     MTEEIAGFQT SPKAQVQAAF EEIARRSMHD LSFLHPSMPV YVSDFTLFEG QWTGCVITPW
     MLSAVIFPGP DQLWPLRKVS EKIGLQLPYG TMTFTVGELD GVSQYLSCSL MSPLSHSMSI
     EEGQRLTDDC ARMILSLPVT NPDVPHAGRR ALLFGRRSGE NA
//