ID RS18_ECOLI Reviewed; 75 AA. AC P0A7T7; P02374; Q2M6A3; DT 21-JUL-1986, integrated into UniProtKB/Swiss-Prot. DT 23-JAN-2007, sequence version 2. DT 19-FEB-2014, entry version 93. DE RecName: Full=30S ribosomal protein S18; GN Name=rpsR; OrderedLocusNames=b4202, JW4160; OS Escherichia coli (strain K12). OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacteriales; OC Enterobacteriaceae; Escherichia. OX NCBI_TaxID=83333; RN [1] RP NUCLEOTIDE SEQUENCE [GENOMIC DNA]. RX PubMed=3528756; DOI=10.1007/BF00330199; RA Schnier J., Kitakawa M., Isono K.; RT "The nucleotide sequence of an Escherichia coli chromosomal region RT containing the genes for ribosomal proteins S6, S18, L9 and an open RT reading frame."; RL Mol. Gen. Genet. 204:126-132(1986). RN [2] RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. RC STRAIN=K12 / MG1655 / ATCC 47076; RX PubMed=7610040; DOI=10.1093/nar/23.12.2105; RA Burland V.D., Plunkett G. III, Sofia H.J., Daniels D.L., RA Blattner F.R.; RT "Analysis of the Escherichia coli genome VI: DNA sequence of the RT region from 92.8 through 100 minutes."; RL Nucleic Acids Res. 23:2105-2119(1995). RN [3] RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. RC STRAIN=K12 / MG1655 / ATCC 47076; RX PubMed=9278503; DOI=10.1126/science.277.5331.1453; RA Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., RA Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., RA Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., RA Mau B., Shao Y.; RT "The complete genome sequence of Escherichia coli K-12."; RL Science 277:1453-1474(1997). RN [4] RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. RC STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911; RX PubMed=16738553; DOI=10.1038/msb4100049; RA Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., RA Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.; RT "Highly accurate genome sequences of Escherichia coli K-12 strains RT MG1655 and W3110."; RL Mol. Syst. Biol. 2:E1-E5(2006). RN [5] RP PROTEIN SEQUENCE OF 2-75. RC STRAIN=K; RX PubMed=776663; DOI=10.1016/0014-5793(75)80378-4; RA Yaguchi M.; RT "Primary structure of protein S18 from the small Escherichia coli RT ribosomal subunit."; RL FEBS Lett. 59:217-220(1975). RN [6] RP PROTEIN SEQUENCE OF 31-38, AND CROSS-LINKING TO RRNA. RC STRAIN=MRE-600; RX PubMed=7556101; RA Urlaub H., Kruft V., Bischof O., Mueller E.-C., Wittmann-Liebold B.; RT "Protein-rRNA binding features and their structural and functional RT implications in ribosomes as determined by cross-linking studies."; RL EMBO J. 14:4578-4588(1995). RN [7] RP MASS SPECTROMETRY. RC STRAIN=K12 / ATCC 25404 / DSM 5698 / NCIMB 11290; RX PubMed=10094780; DOI=10.1006/abio.1998.3077; RA Arnold R.J., Reilly J.P.; RT "Observation of Escherichia coli ribosomal proteins and their RT posttranslational modifications by mass spectrometry."; RL Anal. Biochem. 269:105-112(1999). RN [8] RP 3D-STRUCTURE MODELING. RX PubMed=12244297; DOI=10.1038/nsb841; RA Tung C.-S., Joseph S., Sanbonmatsu K.Y.; RT "All-atom homology model of the Escherichia coli 30S ribosomal RT subunit."; RL Nat. Struct. Biol. 9:750-755(2002). RN [9] RP STRUCTURE BY ELECTRON MICROSCOPY (11.50 ANGSTROMS). RC STRAIN=MRE-600; RX PubMed=12809609; DOI=10.1016/S0092-8674(03)00427-6; RA Gao H., Sengupta J., Valle M., Korostelev A., Eswar N., Stagg S.M., RA Van Roey P., Agrawal R.K., Harvey S.C., Sali A., Chapman M.S., RA Frank J.; RT "Study of the structural dynamics of the E. coli 70S ribosome using RT real-space refinement."; RL Cell 113:789-801(2003). RN [10] RP X-RAY CRYSTALLOGRAPHY (3.46 ANGSTROMS) OF 2 DIFFERENT RIBOSOME RP STRUCTURES. RC STRAIN=MRE-600; RX PubMed=16272117; DOI=10.1126/science.1117230; RA Schuwirth B.S., Borovinskaya M.A., Hau C.W., Zhang W., RA Vila-Sanjurjo A., Holton J.M., Cate J.H.D.; RT "Structures of the bacterial ribosome at 3.5 A resolution."; RL Science 310:827-834(2005). CC -!- FUNCTION: Binds as a heterodimer with protein S6 to the central CC domain of the 16S rRNA, where it helps stabilize the platform of CC the 30S subunit. CC -!- SUBUNIT: Part of the 30S ribosomal subunit. Forms a tight CC heterodimer with protein S6. CC -!- MASS SPECTROMETRY: Mass=8897.0; Method=MALDI; Range=2-75; CC Source=PubMed:10094780; CC -!- SIMILARITY: Belongs to the ribosomal protein S18P family. CC --------------------------------------------------------------------------- CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms CC Distributed under the Creative Commons Attribution (CC BY 4.0) License CC --------------------------------------------------------------------------- DR EMBL; X04022; CAA27654.1; -; Genomic_DNA. DR EMBL; U14003; AAA97098.1; -; Genomic_DNA. DR EMBL; U00096; AAC77159.1; -; Genomic_DNA. DR EMBL; AP009048; BAE78203.1; -; Genomic_DNA. DR PIR; S56427; R3EC18. DR RefSeq; NP_418623.1; NC_000913.3. DR RefSeq; YP_492344.1; NC_007779.1. DR PDB; 1M5G; Model; -; R=5-75. DR PDB; 1P6G; EM; 12.30 A; R=2-75. DR PDB; 1P87; EM; 11.50 A; R=2-75. DR PDB; 1VS5; X-ray; 3.46 A; R=1-75. DR PDB; 1VS7; X-ray; 3.46 A; R=1-75. DR PDB; 2AVY; X-ray; 3.46 A; R=2-75. DR PDB; 2AW7; X-ray; 3.46 A; R=2-75. DR PDB; 2GY9; EM; 15.00 A; R=7-74. DR PDB; 2GYB; EM; 15.00 A; R=7-74. DR PDB; 2I2P; X-ray; 3.22 A; R=2-74. DR PDB; 2I2U; X-ray; 3.22 A; R=2-74. DR PDB; 2QAL; X-ray; 3.21 A; R=2-75. DR PDB; 2QAN; X-ray; 3.21 A; R=2-75. DR PDB; 2QB9; X-ray; 3.54 A; R=2-75. DR PDB; 2QBB; X-ray; 3.54 A; R=2-75. DR PDB; 2QBD; X-ray; 3.30 A; R=2-75. DR PDB; 2QBF; X-ray; 3.30 A; R=2-75. DR PDB; 2QBH; X-ray; 4.00 A; R=2-75. DR PDB; 2QBJ; X-ray; 4.00 A; R=2-75. DR PDB; 2QOU; X-ray; 3.93 A; R=2-75. DR PDB; 2QOW; X-ray; 3.93 A; R=2-75. DR PDB; 2QOY; X-ray; 3.50 A; R=2-75. DR PDB; 2QP0; X-ray; 3.50 A; R=2-75. DR PDB; 2VHO; X-ray; 3.74 A; R=2-75. DR PDB; 2VHP; X-ray; 3.74 A; R=2-75. DR PDB; 2WWL; EM; 5.80 A; R=20-74. DR PDB; 2YKR; EM; 9.80 A; R=20-74. DR PDB; 2Z4K; X-ray; 4.45 A; R=2-75. DR PDB; 2Z4M; X-ray; 4.45 A; R=2-75. DR PDB; 3DF1; X-ray; 3.50 A; R=2-74. DR PDB; 3DF3; X-ray; 3.50 A; R=2-74. DR PDB; 3E1A; EM; -; K=1-75. DR PDB; 3E1C; EM; -; K=1-75. DR PDB; 3FIH; EM; 6.70 A; R=20-74. DR PDB; 3I1M; X-ray; 3.19 A; R=1-75. DR PDB; 3I1O; X-ray; 3.19 A; R=1-75. DR PDB; 3I1Q; X-ray; 3.81 A; R=1-75. DR PDB; 3I1S; X-ray; 3.81 A; R=1-75. DR PDB; 3I1Z; X-ray; 3.71 A; R=1-75. DR PDB; 3I21; X-ray; 3.71 A; R=1-75. DR PDB; 3IZV; EM; -; V=1-75. DR PDB; 3IZW; EM; -; V=1-75. DR PDB; 3J00; EM; -; R=2-75. DR PDB; 3J0U; EM; 12.10 A; U=2-75. DR PDB; 3J0V; EM; 14.70 A; U=2-75. DR PDB; 3J0X; EM; 13.50 A; U=2-75. DR PDB; 3J0Z; EM; 11.50 A; U=2-75. DR PDB; 3J10; EM; 11.50 A; U=2-75. DR PDB; 3J13; EM; 13.10 A; T=2-75. DR PDB; 3J18; EM; 8.30 A; R=20-74. DR PDB; 3J36; EM; 9.80 A; R=2-75. DR PDB; 3J4V; EM; 12.00 A; R=20-74. DR PDB; 3J4W; EM; 12.00 A; R=20-74. DR PDB; 3J4Y; EM; 17.00 A; R=20-74. DR PDB; 3J4Z; EM; 20.00 A; R=20-74. DR PDB; 3J53; EM; 13.00 A; R=20-74. DR PDB; 3J55; EM; 15.00 A; R=20-74. DR PDB; 3J57; EM; 17.00 A; R=20-74. DR PDB; 3J59; EM; 12.00 A; R=20-74. DR PDB; 3J5B; EM; 17.00 A; R=20-74. DR PDB; 3J5D; EM; 17.00 A; R=20-74. DR PDB; 3J5F; EM; 20.00 A; R=20-74. DR PDB; 3J5H; EM; 15.00 A; R=20-74. DR PDB; 3J5J; EM; 9.00 A; R=20-74. DR PDB; 3J5N; EM; 6.80 A; R=1-75. DR PDB; 3J5T; EM; 7.60 A; R=2-75. DR PDB; 3J5X; EM; 7.60 A; R=2-75. DR PDB; 3KC4; EM; -; R=1-75. DR PDB; 3OAQ; X-ray; 3.25 A; R=20-74. DR PDB; 3OAR; X-ray; 3.25 A; R=20-74. DR PDB; 3OFA; X-ray; 3.19 A; R=20-74. DR PDB; 3OFB; X-ray; 3.19 A; R=20-74. DR PDB; 3OFO; X-ray; 3.10 A; R=20-74. DR PDB; 3OFP; X-ray; 3.10 A; R=20-74. DR PDB; 3OFX; X-ray; 3.29 A; R=20-74. DR PDB; 3OFY; X-ray; 3.29 A; R=20-74. DR PDB; 3OR9; X-ray; 3.30 A; R=1-75. DR PDB; 3ORA; X-ray; 3.30 A; R=1-75. DR PDB; 3SFS; X-ray; 3.20 A; R=1-75. DR PDB; 3UOQ; X-ray; 3.70 A; R=1-75. DR PDB; 4A2I; EM; 16.50 A; R=20-74. DR PDB; 4ADV; EM; 13.50 A; R=2-75. DR PDB; 4GAQ; X-ray; 3.30 A; R=1-75. DR PDB; 4GAS; X-ray; 3.30 A; R=1-75. DR PDB; 4GD1; X-ray; 3.00 A; R=20-74. DR PDB; 4GD2; X-ray; 3.00 A; R=20-74. DR PDB; 4KIY; X-ray; 2.90 A; R=1-75. DR PDB; 4KJ0; X-ray; 2.90 A; R=1-75. DR PDB; 4KJ2; X-ray; 2.90 A; R=1-75. DR PDB; 4KJ4; X-ray; 2.90 A; R=1-75. DR PDB; 4KJ6; X-ray; 2.90 A; R=1-75. DR PDB; 4KJ8; X-ray; 2.90 A; R=1-75. DR PDB; 4KJA; X-ray; 2.90 A; R=1-75. DR PDB; 4KJC; X-ray; 2.90 A; R=1-75. DR PDBsum; 1M5G; -. DR PDBsum; 1P6G; -. DR PDBsum; 1P87; -. DR PDBsum; 1VS5; -. DR PDBsum; 1VS7; -. DR PDBsum; 2AVY; -. DR PDBsum; 2AW7; -. DR PDBsum; 2GY9; -. DR PDBsum; 2GYB; -. DR PDBsum; 2I2P; -. DR PDBsum; 2I2U; -. DR PDBsum; 2QAL; -. DR PDBsum; 2QAN; -. DR PDBsum; 2QB9; -. DR PDBsum; 2QBB; -. DR PDBsum; 2QBD; -. DR PDBsum; 2QBF; -. DR PDBsum; 2QBH; -. DR PDBsum; 2QBJ; -. DR PDBsum; 2QOU; -. DR PDBsum; 2QOW; -. DR PDBsum; 2QOY; -. DR PDBsum; 2QP0; -. DR PDBsum; 2VHO; -. DR PDBsum; 2VHP; -. DR PDBsum; 2WWL; -. DR PDBsum; 2YKR; -. DR PDBsum; 2Z4K; -. DR PDBsum; 2Z4M; -. DR PDBsum; 3DF1; -. DR PDBsum; 3DF3; -. DR PDBsum; 3E1A; -. DR PDBsum; 3E1C; -. DR PDBsum; 3FIH; -. DR PDBsum; 3I1M; -. DR PDBsum; 3I1O; -. DR PDBsum; 3I1Q; -. DR PDBsum; 3I1S; -. DR PDBsum; 3I1Z; -. DR PDBsum; 3I21; -. DR PDBsum; 3IZV; -. DR PDBsum; 3IZW; -. DR PDBsum; 3J00; -. DR PDBsum; 3J0U; -. DR PDBsum; 3J0V; -. DR PDBsum; 3J0X; -. DR PDBsum; 3J0Z; -. DR PDBsum; 3J10; -. DR PDBsum; 3J13; -. DR PDBsum; 3J18; -. DR PDBsum; 3J36; -. DR PDBsum; 3J4V; -. DR PDBsum; 3J4W; -. DR PDBsum; 3J4Y; -. DR PDBsum; 3J4Z; -. DR PDBsum; 3J53; -. DR PDBsum; 3J55; -. DR PDBsum; 3J57; -. DR PDBsum; 3J59; -. DR PDBsum; 3J5B; -. DR PDBsum; 3J5D; -. DR PDBsum; 3J5F; -. DR PDBsum; 3J5H; -. DR PDBsum; 3J5J; -. DR PDBsum; 3J5N; -. DR PDBsum; 3J5T; -. DR PDBsum; 3J5X; -. DR PDBsum; 3KC4; -. DR PDBsum; 3OAQ; -. DR PDBsum; 3OAR; -. DR PDBsum; 3OFA; -. DR PDBsum; 3OFB; -. DR PDBsum; 3OFO; -. DR PDBsum; 3OFP; -. DR PDBsum; 3OFX; -. DR PDBsum; 3OFY; -. DR PDBsum; 3OR9; -. DR PDBsum; 3ORA; -. DR PDBsum; 3SFS; -. DR PDBsum; 3UOQ; -. DR PDBsum; 4A2I; -. DR PDBsum; 4ADV; -. DR PDBsum; 4GAQ; -. DR PDBsum; 4GAS; -. DR PDBsum; 4GD1; -. DR PDBsum; 4GD2; -. DR PDBsum; 4KIY; -. DR PDBsum; 4KJ0; -. DR PDBsum; 4KJ2; -. DR PDBsum; 4KJ4; -. DR PDBsum; 4KJ6; -. DR PDBsum; 4KJ8; -. DR PDBsum; 4KJA; -. DR PDBsum; 4KJC; -. DR DisProt; DP00146; -. DR ProteinModelPortal; P0A7T7; -. DR SMR; P0A7T7; 2-75. DR DIP; DIP-47889N; -. DR IntAct; P0A7T7; 24. DR MINT; MINT-1247424; -. DR STRING; 511145.b4202; -. DR ChEMBL; CHEMBL2363135; -. DR PaxDb; P0A7T7; -. DR PRIDE; P0A7T7; -. DR EnsemblBacteria; AAC77159; AAC77159; b4202. DR EnsemblBacteria; BAE78203; BAE78203; BAE78203. DR GeneID; 12930640; -. DR GeneID; 948721; -. DR KEGG; ecj:Y75_p4088; -. DR KEGG; eco:b4202; -. DR PATRIC; 32123979; VBIEscCol129921_4334. DR EchoBASE; EB0910; -. DR EcoGene; EG10917; rpsR. DR eggNOG; COG0238; -. DR HOGENOM; HOG000218466; -. DR KO; K02963; -. DR OMA; RRRYCRF; -. DR OrthoDB; EOG6PCQ4R; -. DR ProtClustDB; PRK00391; -. DR BioCyc; EcoCyc:EG10917-MONOMER; -. DR BioCyc; ECOL316407:JW4160-MONOMER; -. DR EvolutionaryTrace; P0A7T7; -. DR PRO; PR:P0A7T7; -. DR Genevestigator; P0A7T7; -. DR GO; GO:0022627; C:cytosolic small ribosomal subunit; IDA:EcoCyc. DR GO; GO:0019843; F:rRNA binding; IEA:UniProtKB-HAMAP. DR GO; GO:0003735; F:structural constituent of ribosome; IEA:InterPro. DR GO; GO:0006412; P:translation; IEA:UniProtKB-HAMAP. DR Gene3D; 4.10.640.10; -; 1. DR HAMAP; MF_00270; Ribosomal_S18; 1. DR InterPro; IPR001648; Ribosomal_S18. DR InterPro; IPR018275; Ribosomal_S18_CS. DR PANTHER; PTHR13479; PTHR13479; 1. DR Pfam; PF01084; Ribosomal_S18; 1. DR PRINTS; PR00974; RIBOSOMALS18. DR ProDom; PD002239; Ribosomal_S18; 1. DR SUPFAM; SSF46911; SSF46911; 1. DR TIGRFAMs; TIGR00165; S18; 1. DR PROSITE; PS00057; RIBOSOMAL_S18; 1. PE 1: Evidence at protein level; KW 3D-structure; Acetylation; Complete proteome; KW Direct protein sequencing; Reference proteome; Ribonucleoprotein; KW Ribosomal protein; RNA-binding; rRNA-binding. FT INIT_MET 1 1 Removed. FT CHAIN 2 75 30S ribosomal protein S18. FT /FTId=PRO_0000111153. FT MOD_RES 2 2 N-acetylalanine. FT CONFLICT 24 24 K -> E (in Ref. 5; AA sequence). FT STRAND 22 24 FT HELIX 26 29 FT HELIX 30 32 FT STRAND 37 39 FT HELIX 42 45 FT HELIX 49 63 FT TURN 64 66 FT STRAND 70 72 SQ SEQUENCE 75 AA; 8986 MW; AB2E8288901B73B8 CRC64; MARYFRRRKF CRFTAEGVQE IDYKDIATLK NYITESGKIV PSRITGTRAK YQRQLARAIK RARYLSLLPY TDRHQ //