ID RS18_ECOLI Reviewed; 75 AA. AC P0A7T7; P02374; Q2M6A3; DT 21-JUL-1986, integrated into UniProtKB/Swiss-Prot. DT 23-JAN-2007, sequence version 2. DT 19-JAN-2010, entry version 56. DE RecName: Full=30S ribosomal protein S18; GN Name=rpsR; OrderedLocusNames=b4202, JW4160; OS Escherichia coli (strain K12). OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacteriales; OC Enterobacteriaceae; Escherichia. OX NCBI_TaxID=83333; RN [1] RP NUCLEOTIDE SEQUENCE [GENOMIC DNA]. RX MEDLINE=86310297; PubMed=3528756; DOI=10.1007/BF00330199; RA Schnier J., Kitakawa M., Isono K.; RT "The nucleotide sequence of an Escherichia coli chromosomal region RT containing the genes for ribosomal proteins S6, S18, L9 and an open RT reading frame."; RL Mol. Gen. Genet. 204:126-132(1986). RN [2] RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. RC STRAIN=K12 / MG1655 / ATCC 47076; RX MEDLINE=95334362; PubMed=7610040; DOI=10.1093/nar/23.12.2105; RA Burland V.D., Plunkett G. III, Sofia H.J., Daniels D.L., RA Blattner F.R.; RT "Analysis of the Escherichia coli genome VI: DNA sequence of the RT region from 92.8 through 100 minutes."; RL Nucleic Acids Res. 23:2105-2119(1995). RN [3] RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. RC STRAIN=K12 / MG1655 / ATCC 47076; RX MEDLINE=97426617; PubMed=9278503; DOI=10.1126/science.277.5331.1453; RA Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., RA Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., RA Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., RA Mau B., Shao Y.; RT "The complete genome sequence of Escherichia coli K-12."; RL Science 277:1453-1474(1997). RN [4] RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. RC STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911; RX PubMed=16738553; DOI=10.1038/msb4100049; RA Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., RA Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.; RT "Highly accurate genome sequences of Escherichia coli K-12 strains RT MG1655 and W3110."; RL Mol. Syst. Biol. 2:E1-E5(2006). RN [5] RP PROTEIN SEQUENCE OF 2-75. RC STRAIN=K; RX MEDLINE=76210737; PubMed=776663; DOI=10.1016/0014-5793(75)80378-4; RA Yaguchi M.; RT "Primary structure of protein S18 from the small Escherichia coli RT ribosomal subunit."; RL FEBS Lett. 59:217-220(1975). RN [6] RP PROTEIN SEQUENCE OF 31-38, AND CROSS-LINKING TO RRNA. RC STRAIN=MRE-600; RX MEDLINE=96003638; PubMed=7556101; RA Urlaub H., Kruft V., Bischof O., Mueller E.-C., Wittmann-Liebold B.; RT "Protein-rRNA binding features and their structural and functional RT implications in ribosomes as determined by cross-linking studies."; RL EMBO J. 14:4578-4588(1995). RN [7] RP MASS SPECTROMETRY. RC STRAIN=K12 / ATCC 25404 / DSM 5698 / NCIMB 11290; RX MEDLINE=99196679; PubMed=10094780; DOI=10.1006/abio.1998.3077; RA Arnold R.J., Reilly J.P.; RT "Observation of Escherichia coli ribosomal proteins and their RT posttranslational modifications by mass spectrometry."; RL Anal. Biochem. 269:105-112(1999). RN [8] RP 3D-STRUCTURE MODELING. RX MEDLINE=22239879; PubMed=12244297; DOI=10.1038/nsb841; RA Tung C.-S., Joseph S., Sanbonmatsu K.Y.; RT "All-atom homology model of the Escherichia coli 30S ribosomal RT subunit."; RL Nat. Struct. Biol. 9:750-755(2002). RN [9] RP STRUCTURE BY ELECTRON MICROSCOPY (11.50 ANGSTROMS). RC STRAIN=MRE-600; RX MEDLINE=22694394; PubMed=12809609; DOI=10.1016/S0092-8674(03)00427-6; RA Gao H., Sengupta J., Valle M., Korostelev A., Eswar N., Stagg S.M., RA Van Roey P., Agrawal R.K., Harvey S.C., Sali A., Chapman M.S., RA Frank J.; RT "Study of the structural dynamics of the E. coli 70S ribosome using RT real-space refinement."; RL Cell 113:789-801(2003). RN [10] RP X-RAY CRYSTALLOGRAPHY (3.46 ANGSTROMS) OF 2 DIFFERENT RIBOSOME RP STRUCTURES. RC STRAIN=MRE-600; RX PubMed=16272117; DOI=10.1126/science.1117230; RA Schuwirth B.S., Borovinskaya M.A., Hau C.W., Zhang W., RA Vila-Sanjurjo A., Holton J.M., Cate J.H.D.; RT "Structures of the bacterial ribosome at 3.5 A resolution."; RL Science 310:827-834(2005). CC -!- FUNCTION: Binds as a heterodimer with protein S6 to the central CC domain of the 16S rRNA, where it helps stabilize the platform of CC the 30S subunit. CC -!- SUBUNIT: Part of the 30S ribosomal subunit. Forms a tight CC heterodimer with protein S6. CC -!- MASS SPECTROMETRY: Mass=8897.0; Method=MALDI; Range=2-75; CC Source=PubMed:10094780; CC -!- SIMILARITY: Belongs to the ribosomal protein S18P family. CC --------------------------------------------------------------------------- CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms CC Distributed under the Creative Commons Attribution (CC BY 4.0) License CC --------------------------------------------------------------------------- DR EMBL; X04022; CAA27654.1; -; Genomic_DNA. DR EMBL; U14003; AAA97098.1; -; Genomic_DNA. DR EMBL; U00096; AAC77159.1; -; Genomic_DNA. DR EMBL; AP009048; BAE78203.1; -; Genomic_DNA. DR PIR; S56427; R3EC18. DR RefSeq; AP_004702.1; -. DR RefSeq; NP_418623.1; -. DR PDB; 1M5G; Model; -; R=5-75. DR PDB; 1P6G; EM; 12.30 A; R=2-75. DR PDB; 1P87; EM; 11.50 A; R=2-75. DR PDB; 1VS5; X-ray; 3.46 A; R=1-75. DR PDB; 1VS7; X-ray; 3.46 A; R=1-75. DR PDB; 2AVY; X-ray; 3.46 A; R=2-75. DR PDB; 2AW7; X-ray; 3.46 A; R=2-75. DR PDB; 2GY9; EM; 15.00 A; R=7-74. DR PDB; 2GYB; EM; 15.00 A; R=7-74. DR PDB; 2I2P; X-ray; 3.22 A; R=2-74. DR PDB; 2I2U; X-ray; 3.22 A; R=2-74. DR PDB; 2QAL; X-ray; 3.21 A; R=2-75. DR PDB; 2QAN; X-ray; 3.21 A; R=2-75. DR PDB; 2QB9; X-ray; 3.54 A; R=2-75. DR PDB; 2QBB; X-ray; 3.54 A; R=2-75. DR PDB; 2QBD; X-ray; 3.30 A; R=2-75. DR PDB; 2QBF; X-ray; 3.30 A; R=2-75. DR PDB; 2QBH; X-ray; 4.00 A; R=2-75. DR PDB; 2QBJ; X-ray; 4.00 A; R=2-75. DR PDB; 2QOU; X-ray; 3.93 A; R=2-75. DR PDB; 2QOW; X-ray; 3.93 A; R=2-75. DR PDB; 2QOY; X-ray; 3.50 A; R=2-75. DR PDB; 2QP0; X-ray; 3.50 A; R=2-75. DR PDB; 2VHO; X-ray; 3.74 A; R=2-75. DR PDB; 2VHP; X-ray; 3.74 A; R=2-75. DR PDB; 2Z4K; X-ray; 4.45 A; R=2-75. DR PDB; 2Z4M; X-ray; 4.45 A; R=2-75. DR PDB; 3DF1; X-ray; 3.50 A; R=2-74. DR PDB; 3DF3; X-ray; 3.50 A; R=2-74. DR PDB; 3E1A; EM; -; K=1-75. DR PDB; 3E1C; EM; -; K=1-75. DR PDB; 3FIH; EM; 6.70 A; R=20-74. DR PDB; 3I1M; X-ray; 3.19 A; R=1-75. DR PDB; 3I1O; X-ray; 3.19 A; R=1-75. DR PDB; 3I1Q; X-ray; 3.81 A; R=1-75. DR PDB; 3I1S; X-ray; 3.81 A; R=1-75. DR PDB; 3I1Z; X-ray; 3.71 A; R=1-75. DR PDB; 3I21; X-ray; 3.71 A; R=1-75. DR PDBsum; 1M5G; -. DR PDBsum; 1P6G; -. DR PDBsum; 1P87; -. DR PDBsum; 1VS5; -. DR PDBsum; 1VS7; -. DR PDBsum; 2AVY; -. DR PDBsum; 2AW7; -. DR PDBsum; 2GY9; -. DR PDBsum; 2GYB; -. DR PDBsum; 2I2P; -. DR PDBsum; 2I2U; -. DR PDBsum; 2QAL; -. DR PDBsum; 2QAN; -. DR PDBsum; 2QB9; -. DR PDBsum; 2QBB; -. DR PDBsum; 2QBD; -. DR PDBsum; 2QBF; -. DR PDBsum; 2QBH; -. DR PDBsum; 2QBJ; -. DR PDBsum; 2QOU; -. DR PDBsum; 2QOW; -. DR PDBsum; 2QOY; -. DR PDBsum; 2QP0; -. DR PDBsum; 2VHO; -. DR PDBsum; 2VHP; -. DR PDBsum; 2Z4K; -. DR PDBsum; 2Z4M; -. DR PDBsum; 3DF1; -. DR PDBsum; 3DF3; -. DR PDBsum; 3E1A; -. DR PDBsum; 3E1C; -. DR PDBsum; 3FIH; -. DR PDBsum; 3I1M; -. DR PDBsum; 3I1O; -. DR PDBsum; 3I1Q; -. DR PDBsum; 3I1S; -. DR PDBsum; 3I1Z; -. DR PDBsum; 3I21; -. DR STRING; P0A7T7; -. DR GeneID; 948721; -. DR GenomeReviews; AP009048_GR; JW4160. DR GenomeReviews; U00096_GR; b4202. DR KEGG; ecj:JW4160; -. DR KEGG; eco:b4202; -. DR EchoBASE; EB0910; -. DR EcoGene; EG10917; rpsR. DR eggNOG; COG0238; -. DR HOGENOM; HBG608793; -. DR OMA; SARYQRQ; -. DR BioCyc; EcoCyc:EG10917-MONOMER; -. DR BioCyc; ECOL168927:B4202-MONOMER; -. DR Genevestigator; P0A7T7; -. DR GO; GO:0005840; C:ribosome; IEA:UniProtKB-KW. DR GO; GO:0019843; F:rRNA binding; IEA:HAMAP. DR GO; GO:0003735; F:structural constituent of ribosome; IEA:InterPro. DR GO; GO:0006412; P:translation; IEA:HAMAP. DR HAMAP; MF_00270; Ribosomal_S18; 1; -. DR InterPro; IPR001648; Ribosomal_S18. DR InterPro; IPR018275; Ribosomal_S18_CS. DR Gene3D; G3DSA:4.10.640.10; Ribosomal_S18; 1. DR PANTHER; PTHR13479; Ribosomal_S18; 1. DR Pfam; PF01084; Ribosomal_S18; 1. DR PRINTS; PR00974; RIBOSOMALS18. DR ProDom; PD002239; Ribosomal_S18; 1. DR PROSITE; PS00057; RIBOSOMAL_S18; 1. PE 1: Evidence at protein level; KW 3D-structure; Acetylation; Complete proteome; KW Direct protein sequencing; Ribonucleoprotein; Ribosomal protein; KW RNA-binding; rRNA-binding. FT INIT_MET 1 1 Removed. FT CHAIN 2 75 30S ribosomal protein S18. FT /FTId=PRO_0000111153. FT MOD_RES 2 2 N-acetylalanine. FT CONFLICT 24 24 K -> E (in Ref. 5; AA sequence). FT STRAND 22 24 FT HELIX 26 28 FT HELIX 30 32 FT TURN 43 45 FT HELIX 50 64 SQ SEQUENCE 75 AA; 8986 MW; AB2E8288901B73B8 CRC64; MARYFRRRKF CRFTAEGVQE IDYKDIATLK NYITESGKIV PSRITGTRAK YQRQLARAIK RARYLSLLPY TDRHQ //