ID RS18_ECOLI STANDARD; PRT; 74 AA. AC P0A7T7; P02374; DT 21-JUL-1986, integrated into UniProtKB/Swiss-Prot. DT 01-APR-1988, sequence version 1. DT 04-APR-2006, entry version 12. DE 30S ribosomal protein S18. GN Name=rpsR; OrderedLocusNames=b4202; OS Escherichia coli. OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacteriales; OC Enterobacteriaceae; Escherichia. OX NCBI_TaxID=562; RN [1] RP PROTEIN SEQUENCE. RC STRAIN=K; RX MEDLINE=76210737; PubMed=776663; DOI=10.1016/0014-5793(75)80378-4; RA Yaguchi M.; RT "Primary structure of protein S18 from the small Escherichia coli RT ribosomal subunit."; RL FEBS Lett. 59:217-220(1975). RN [2] RP NUCLEOTIDE SEQUENCE [GENOMIC DNA]. RX MEDLINE=86310297; PubMed=3528756; DOI=10.1007/BF00330199; RA Schnier J., Kitakawa M., Isono K.; RT "The nucleotide sequence of an Escherichia coli chromosomal region RT containing the genes for ribosomal proteins S6, S18, L9 and an open RT reading frame."; RL Mol. Gen. Genet. 204:126-132(1986). RN [3] RP NUCLEOTIDE SEQUENCE [GENOMIC DNA]. RC STRAIN=K12 / MG1655; RX MEDLINE=95334362; PubMed=7610040; RA Burland V.D., Plunkett G. III, Sofia H.J., Daniels D.L., RA Blattner F.R.; RT "Analysis of the Escherichia coli genome VI: DNA sequence of the RT region from 92.8 through 100 minutes."; RL Nucleic Acids Res. 23:2105-2119(1995). RN [4] RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. RC STRAIN=K12 / MG1655; RX MEDLINE=97426617; PubMed=9278503; DOI=10.1126/science.277.5331.1453; RA Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., RA Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., RA Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., RA Mau B., Shao Y.; RT "The complete genome sequence of Escherichia coli K-12."; RL Science 277:1453-1474(1997). RN [5] RP PROTEIN SEQUENCE OF 30-37, AND CROSS-LINKING TO RRNA. RC STRAIN=MRE-600; RX MEDLINE=96003638; PubMed=7556101; RA Urlaub H., Kruft V., Bischof O., Mueller E.-C., Wittmann-Liebold B.; RT "Protein-rRNA binding features and their structural and functional RT implications in ribosomes as determined by cross-linking studies."; RL EMBO J. 14:4578-4588(1995). RN [6] RP MASS SPECTROMETRY. RC STRAIN=K12 / ATCC 25404; RX MEDLINE=99196679; PubMed=10094780; DOI=10.1006/abio.1998.3077; RA Arnold R.J., Reilly J.P.; RT "Observation of Escherichia coli ribosomal proteins and their RT posttranslational modifications by mass spectrometry."; RL Anal. Biochem. 269:105-112(1999). RN [7] RP 3D-STRUCTURE MODELING. RX MEDLINE=22239879; PubMed=12244297; DOI=10.1038/nsb841; RA Tung C.-S., Joseph S., Sanbonmatsu K.Y.; RT "All-atom homology model of the Escherichia coli 30S ribosomal RT subunit."; RL Nat. Struct. Biol. 9:750-755(2002). RN [8] RP STRUCTURE BY ELECTRON MICROSCOPY (11.50 ANGSTROMS). RC STRAIN=MRE-600; RX MEDLINE=22694394; PubMed=12809609; DOI=10.1016/S0092-8674(03)00427-6; RA Gao H., Sengupta J., Valle M., Korostelev A., Eswar N., Stagg S.M., RA Van Roey P., Agrawal R.K., Harvey S.C., Sali A., Chapman M.S., RA Frank J.; RT "Study of the structural dynamics of the E. coli 70S ribosome using RT real-space refinement."; RL Cell 113:789-801(2003). RN [9] RP X-RAY CRYSTALLOGRAPHY (3.46 ANGSTROMS) OF 2 DIFFERENT RIBOSOME RP STRUCTURES. RC STRAIN=MRE-600; RX PubMed=16272117; DOI=10.1126/science.1117230; RA Schuwirth B.S., Borovinskaya M.A., Hau C.W., Zhang W., RA Vila-Sanjurjo A., Holton J.M., Cate J.H.D.; RT "Structures of the bacterial ribosome at 3.5 A resolution."; RL Science 310:827-834(2005). CC -!- FUNCTION: Binds as a heterodimer with protein S6 to the central CC domain of the 16S rRNA, where it helps stabilize the platform of CC the 30S subunit. CC -!- SUBUNIT: Part of the 30S ribosomal subunit. Forms a tight CC heterodimer with protein S6. CC -!- INTERACTION: CC P23304:deaD; NbExp=1; IntAct=EBI-548844, EBI-546193; CC P0A6Q3:fabA; NbExp=1; IntAct=EBI-548844, EBI-369375; CC P04742:fimB; NbExp=1; IntAct=EBI-548844, EBI-550135; CC P0A6X3:hfq; NbExp=1; IntAct=EBI-548844, EBI-547637; CC P0A707:infC; NbExp=1; IntAct=EBI-548844, EBI-546262; CC P21645:lpxD; NbExp=1; IntAct=EBI-548844, EBI-542924; CC P68739:nfi; NbExp=1; IntAct=EBI-548844, EBI-551698; CC P0A776:nudH; NbExp=1; IntAct=EBI-548844, EBI-562338; CC P05055:pnp; NbExp=1; IntAct=EBI-548844, EBI-548080; CC P37765:rluB; NbExp=1; IntAct=EBI-548844, EBI-561550; CC P23851:rluC; NbExp=1; IntAct=EBI-548844, EBI-561528; CC P0A7Y8:rnpA; NbExp=1; IntAct=EBI-548844, EBI-557396; CC P21499:rnr; NbExp=1; IntAct=EBI-548844, EBI-548136; CC P17580:spoT; NbExp=1; IntAct=EBI-548844, EBI-543228; CC P0A8G0:uvrC; NbExp=1; IntAct=EBI-548844, EBI-559448; CC P36979:yfgB; NbExp=1; IntAct=EBI-548844, EBI-559071; CC P33635:yfiF; NbExp=1; IntAct=EBI-548844, EBI-547368; CC -!- MASS SPECTROMETRY: MW=8897.0; METHOD=MALDI; RANGE=1-74; CC NOTE=Ref.6. CC -!- SIMILARITY: Belongs to the ribosomal protein S18P family. CC --------------------------------------------------------------------------- CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms CC Distributed under the Creative Commons Attribution (CC BY 4.0) License CC --------------------------------------------------------------------------- DR EMBL; X04022; CAA27654.1; -; Genomic_DNA. DR EMBL; U14003; AAA97098.1; -; Genomic_DNA. DR EMBL; U00096; AAC77159.1; -; Genomic_DNA. DR PIR; S56427; R3EC18. DR PDB; 1P6G; EM; R=1-74. DR PDB; 1P87; EM; R=1-74. DR PDB; 2AVY; X-ray; R=1-74. DR PDB; 2AW7; X-ray; R=1-74. DR IntAct; P0A7T7; -. DR GenomeReviews; U00096_GR; b4202. DR EchoBASE; EB0910; -. DR EcoGene; EG10917; rpsR. DR HAMAP; MF_00270; -; 1. DR InterPro; IPR001648; Ribosomal_S18. DR Pfam; PF01084; Ribosomal_S18; 1. DR PRINTS; PR00974; RIBOSOMALS18. DR ProDom; PD002239; Ribosomal_S18; 1. DR TIGRFAMs; TIGR00165; S18; 1. DR PROSITE; PS00057; RIBOSOMAL_S18; 1. KW 3D-structure; Acetylation; Complete proteome; KW Direct protein sequencing; Ribonucleoprotein; Ribosomal protein; KW RNA-binding; rRNA-binding. FT INIT_MET 0 0 FT CHAIN 1 74 30S ribosomal protein S18. FT /FTId=PRO_0000111153. FT MOD_RES 1 1 N-acetylalanine. FT CONFLICT 23 23 K -> E (in Ref. 1). FT STRAND 21 23 FT HELIX 25 27 FT TURN 28 30 FT STRAND 31 32 FT TURN 34 35 FT STRAND 41 41 FT TURN 42 44 FT TURN 47 48 FT HELIX 49 63 FT TURN 64 65 FT STRAND 66 67 FT STRAND 69 69 FT STRAND 71 71 SQ SEQUENCE 74 AA; 8855 MW; AB2EDDEEA9441581 CRC64; ARYFRRRKFC RFTAEGVQEI DYKDIATLKN YITESGKIVP SRITGTRAKY QRQLARAIKR ARYLSLLPYT DRHQ //