ID TDT_MOUSE STANDARD; PRT; 530 AA. AC P09838; Q99PD0; Q99PD1; DT 01-MAR-1989 (Rel. 10, Created) DT 01-MAR-2002 (Rel. 41, Last sequence update) DT 01-MAR-2002 (Rel. 41, Last annotation update) DE DNA nucleotidylexotransferase (EC 2.7.7.31) (Terminal addition enzyme) DE (Terminal deoxynucleotidyltransferase) (TDT) (Terminal transferase). GN DNTT OR TDT. OS Mus musculus (Mouse). OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; OC Mammalia; Eutheria; Rodentia; Sciurognathi; Muridae; Murinae; Mus. OX NCBI_TaxID=10090; RN [1] RP SEQUENCE FROM N.A. RX MEDLINE=86286588; PubMed=3755527; RA Koiwai O., Yokota T., Kageyama T., Hirose T., Yoshida S., Arai K.; RT "Isolation and characterization of bovine and mouse terminal RT deoxynucleotidyltransferase cDNAs expressible in mammalian cells."; RL Nucleic Acids Res. 14:5777-5792(1986). RN [2] RP SEQUENCE FROM N.A. RC STRAIN=BALB/c; TISSUE=Thymus; RX MEDLINE=93219079; PubMed=8464703; RA Doyen N., Fanton D'Andon M., Bentolila L.A., Nguyen T.Q., Rougeon F.; RT "Differential splicing in mouse thymus generates two forms of RT terminal deoxynucleotidyl transferase."; RL Nucleic Acids Res. 21:1187-1191(1993). RN [3] RP SEQUENCE FROM N.A. (ISOFORMS 1 AND 2), AND CHARACTERIZATION. RC STRAIN=C57BL/6; TISSUE=Thymus; RX PubMed=11136823; RA Benedict C.L., Gilfillan S., Kearney J.F.; RT "The long isoform of terminal deoxynucleotidyl transferase (TdtL) RT enters the nucleus and, rather than catalyzing N addition, modulates RT the catalytic activity of the short isoform."; RL J. Exp. Med. 193:89-99(2001). RN [4] RP PRELIMINARY CHARACTERIZATION OF ALTERNATIVE FORMS. RX MEDLINE=96016194; PubMed=7556063; RA Bentolila L.A., Fanton D'Andon M., Nguyen T.Q., Martinez O., RA Rougeon F., Doyen N.; RT "The two isoforms of mouse terminal deoxynucleotidyl transferase RT differ in both the ability to add N regions and subcellular RT localization."; RL EMBO J. 14:4221-4229(1995). CC -!- FUNCTION: TEMPLATE-INDEPENDENT DNA POLYMERASE WHICH CATALYZES THE CC RANDOM ADDITION OF DEOXYNUCLEOSIDE 5'-TRIPHOSPHATE TO THE 3'END OF CC A DNA INITIATOR. ONE OF THE IN-VIVO FUNCTION OF THIS ENZYME IS THE CC ADDITION OF NUCLEOTIDES AT THE JUNCTION (N REGION) OF REARRANGED CC IG HEAVY CHAIN AND T CELL RECEPTOR GENE SEGMENTS DURING THE CC MATURATION OF B AND T CELLS. The TdtL isoform seems to serve to CC modulate the function of TdtS. CC -!- CATALYTIC ACTIVITY: N deoxynucleoside triphosphate + CC {deoxynucleotide}(M) = N diphosphate + {deoxynucleotide}(M+N). CC -!- COFACTOR: REQUIRES MAGNESIUM. CC -!- SUBCELLULAR LOCATION: Nuclear. CC -!- ALTERNATIVE PRODUCTS: 2 isoforms; TDT-L(arge)/TdtL (shown here) CC and TDT-S(mall)/TdtS; are produced by alternative splicing. The CC TDT-S form is the major form. CC -!- SIMILARITY: BELONGS TO DNA POLYMERASE TYPE-X FAMILY. CC -!- SIMILARITY: CONTAINS 1 BRCT DOMAIN. CC --------------------------------------------------------------------------- CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms CC Distributed under the Creative Commons Attribution (CC BY 4.0) License CC --------------------------------------------------------------------------- DR EMBL; X04123; CAA27735.1; -. DR EMBL; X68670; CAA48634.1; -. DR EMBL; AF316014; AAK07884.1; -. DR EMBL; AF316015; AAK07885.1; -. DR PIR; B23595; B23595. DR HSSP; P06766; 1BPB. DR MGD; MGI:98659; Dntt. DR InterPro; IPR001357; BRCT. DR InterPro; IPR002054; DNA_polX. DR InterPro; IPR002934; NTP_transf. DR Pfam; PF00533; BRCT; 1. DR Pfam; PF01909; NTP_transf_2; 1. DR PRINTS; PR00869; DNAPOLX. DR SMART; SM00292; BRCT; 1. DR SMART; SM00483; POLXc; 1. DR PROSITE; PS50172; BRCT; 1. DR PROSITE; PS00522; DNA_POLYMERASE_X; 1. KW Transferase; Nucleotidyltransferase; Terminal addition; Magnesium; KW Nuclear protein; Alternative splicing. FT DOMAIN 27 118 BRCT. FT VARSPLIC 483 502 MISSING (IN ISOFORM TDT-S). FT CONFLICT 26 26 T -> M (IN REF. 2). FT CONFLICT 99 99 L -> F (IN REF. 2). FT CONFLICT 193 193 R -> G (IN REF. 1). FT CONFLICT 287 287 Q -> K (IN REF. 1). FT CONFLICT 309 309 E -> Q (IN REF. 1). FT CONFLICT 367 367 D -> H (IN REF. 1). FT CONFLICT 441 444 DRRA -> ECAC (IN REF. 1). FT CONFLICT 443 445 RAF -> AS (IN REF. 2). SQ SEQUENCE 530 AA; 60331 MW; E6B109DCF39C8107 CRC64; MDPLQAVHLG PRKKRPRQLG TPVASTPYDI RFRDLVLFIL EKKMGTTRRA FLMELARRKG FRVENELSDS VTHIVAENNS GSDVLEWLQL QNIKASSELE LLDISWLIEC MGAGKPVEMM GRHQLVVNRN SSPSPVPGSQ NVPAPAVKKI SQYACQRRTT LNNYNQLFTD ALDILAENDE LRENEGSCLA FMRASSVLKS LPFPITSMKD TEGIPCLGDK VKSIIEGIIE DGESSEAKAV LNDERYKSFK LFTSVFGVGL KTAEKWFRMG FRTLSKIQSD KSLRFTQMQK AGFLYYEDLV SCVNRPEAEA VSMLVKEAVV TFLPDALVTM TGGFRRGKMT GHDVDFLITS PEATEDEEQQ LLHKVTDFWK QQGLLLYCDI LESTFEKFKQ PSRKVDALDH FQKCFLILKL DHGRVHSEKS GQQEGKGWKA IRVDLVMCPY DRRAFALLGW TGSRQFERDL RRYATHERKM MLDNHALYDR TKGKTVTISP LDGKVSKLQK ALRVFLEAES EEEIFAHLGL DYIEPWERNA //