ID US27_HCMVA Reviewed; 362 AA. AC P09703; Q7M6H4; DT 01-JUL-1989, integrated into UniProtKB/Swiss-Prot. DT 01-JUL-1989, sequence version 1. DT 03-OCT-2012, entry version 82. DE RecName: Full=G-protein coupled receptor homolog US27; DE AltName: Full=HHRF2; GN Name=US27; OS Human cytomegalovirus (strain AD169) (HHV-5) (Human herpesvirus 5). OC Viruses; dsDNA viruses, no RNA stage; Herpesvirales; Herpesviridae; OC Betaherpesvirinae; Cytomegalovirus. OX NCBI_TaxID=10360; OH NCBI_TaxID=9606; Homo sapiens (Human). RN [1] RP NUCLEOTIDE SEQUENCE [GENOMIC DNA]. RX MEDLINE=87169717; PubMed=3031311; DOI=10.1016/0022-2836(86)90359-1; RA Weston K.M., Barrell B.G.; RT "Sequence of the short unique region, short repeats, and part of the RT long repeats of human cytomegalovirus."; RL J. Mol. Biol. 192:177-208(1986). RN [2] RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. RX MEDLINE=90269039; PubMed=2161319; RA Chee M.S., Bankier A.T., Beck S., Bohni R., Brown C.M., Cerny R., RA Horsnell T., Hutchison C.A. III, Kouzarides T., Martignetti J.A., RA Preddie E., Satchwell S.C., Tomlinson P., Weston K.M., Barrell B.G.; RT "Analysis of the protein-coding content of the sequence of human RT cytomegalovirus strain AD169."; RL Curr. Top. Microbiol. Immunol. 154:125-169(1990). RN [3] RP SIMILARITY TO G-PROTEIN COUPLED RECEPTORS. RX MEDLINE=90231415; PubMed=2158627; DOI=10.1038/344774a0; RA Chee M.S., Satchwell S.C., Preddie E., Weston K.M., Barrell B.G.; RT "Human cytomegalovirus encodes three G protein-coupled receptor RT homologues."; RL Nature 344:774-777(1990). RN [4] RP GENOME REANNOTATION. RX MEDLINE=22421467; PubMed=12533697; DOI=10.1099/vir.0.18606-0; RA Davison A.J., Dolan A., Akter P., Addison C., Dargan D.J., RA Alcendor D.J., McGeoch D.J., Hayward G.S.; RT "The human cytomegalovirus genome revisited: comparison with the RT chimpanzee cytomegalovirus genome."; RL J. Gen. Virol. 84:17-28(2003). RN [5] RP ERRATUM. RA Davison A.J., Dolan A., Akter P., Addison C., Dargan D.J., RA Alcendor D.J., McGeoch D.J., Hayward G.S.; RL J. Gen. Virol. 84:1053-1053(2003). RN [6] RP SUBCELLULAR LOCATION. RX PubMed=11886592; DOI=10.1034/j.1600-0854.2002.030307.x; RA Fraile-Ramos A., Pelchen-Matthews A., Kledal T.N., Browne H., RA Schwartz T.W., Marsh M.; RT "Localization of HCMV UL33 and US27 in endocytic compartments and RT viral membranes."; RL Traffic 3:218-232(2002). RN [7] RP IDENTIFICATION. RX PubMed=15452216; DOI=10.1128/JVI.78.20.10960-10966.2004; RA Varnum S.M., Streblow D.N., Monroe M.E., Smith P., Auberry K.J., RA Pasa-Tolic L., Wang D., Camp D.G. II, Rodland K., Wiley S., Britt W., RA Shenk T., Smith R.D., Nelson J.A.; RT "Identification of proteins in human cytomegalovirus (HCMV) particles: RT the HCMV proteome."; RL J. Virol. 78:10960-10966(2004). RN [8] RP ERRATUM. RA Varnum S.M., Streblow D.N., Monroe M.E., Smith P., Auberry K.J., RA Pasa-Tolic L., Wang D., Camp D.G. II, Rodland K., Wiley S., Britt W., RA Shenk T., Smith R.D., Nelson J.A.; RL J. Virol. 78:13395-13395(2004). RN [9] RP INTERACTION WITH US28. RX PubMed=21684267; DOI=10.1016/j.bcp.2011.06.009; RA Tschische P., Tadagaki K., Kamal M., Jockers R., Waldhoer M.; RT "Heteromerization of human cytomegalovirus encoded chemokine RT receptors."; RL Biochem. Pharmacol. 82:610-619(2011). CC -!- FUNCTION: Plays an important role in spread of HCMV via the CC extracellular route. As a G-protein-coupled receptor (vGPCR), may CC activate signaling pathways important for virion assembly or CC egress processes. CC -!- SUBUNIT: Heterodimerizes with US28. CC -!- SUBCELLULAR LOCATION: Virion. Host cell membrane; Multi-pass CC membrane protein. CC -!- SIMILARITY: Belongs to the G-protein coupled receptor 1 family. CC --------------------------------------------------------------------------- CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms CC Distributed under the Creative Commons Attribution (CC BY 4.0) License CC --------------------------------------------------------------------------- DR EMBL; X17403; CAA35259.1; -; Genomic_DNA. DR EMBL; X04650; CAA28337.1; -; Genomic_DNA. DR EMBL; BK000394; DAA00214.1; -; Genomic_DNA. DR PIR; B27216; QQBED2. DR ProteinModelPortal; P09703; -. DR GO; GO:0020002; C:host cell plasma membrane; IEA:UniProtKB-SubCell. DR GO; GO:0016021; C:integral to membrane; IEA:UniProtKB-KW. DR GO; GO:0019012; C:virion; IEA:UniProtKB-SubCell. DR GO; GO:0004930; F:G-protein coupled receptor activity; IEA:UniProtKB-KW. DR GO; GO:0030683; P:evasion by virus of host immune response; IEA:UniProtKB-KW. DR GO; GO:0039553; P:suppression by virus of host chemokine activity; IEA:UniProtKB-KW. DR InterPro; IPR000276; 7TM_GPCR_Rhodpsn. DR InterPro; IPR017452; GPCR_Rhodpsn_supfam. DR Pfam; PF00001; 7tm_1; 1. DR PRINTS; PR00237; GPCRRHODOPSN. DR PROSITE; PS00237; G_PROTEIN_RECEP_F1_1; 1. DR PROSITE; PS50262; G_PROTEIN_RECEP_F1_2; 1. PE 1: Evidence at protein level; KW Complete proteome; G-protein coupled receptor; Glycoprotein; KW Host cell membrane; Host membrane; Host-virus interaction; KW Inhibition of host chemokines by virus; Membrane; Receptor; KW Transducer; Transmembrane; Transmembrane helix; Viral immunoevasion; KW Virion. FT CHAIN 1 362 G-protein coupled receptor homolog US27. FT /FTId=PRO_0000070245. FT TOPO_DOM 1 34 Virion surface (Potential). FT TRANSMEM 35 58 Helical; Name=1; (Potential). FT TOPO_DOM 59 67 Intravirion (Potential). FT TRANSMEM 68 90 Helical; Name=2; (Potential). FT TOPO_DOM 91 104 Virion surface (Potential). FT TRANSMEM 105 126 Helical; Name=3; (Potential). FT TOPO_DOM 127 148 Intravirion (Potential). FT TRANSMEM 149 167 Helical; Name=4; (Potential). FT TOPO_DOM 168 193 Virion surface (Potential). FT TRANSMEM 194 213 Helical; Name=5; (Potential). FT TOPO_DOM 214 233 Intravirion (Potential). FT TRANSMEM 234 257 Helical; Name=6; (Potential). FT TOPO_DOM 258 274 Virion surface (Potential). FT TRANSMEM 275 298 Helical; Name=7; (Potential). FT TOPO_DOM 299 362 Intravirion (Potential). FT CARBOHYD 7 7 N-linked (GlcNAc...); by host FT (Potential). FT CARBOHYD 15 15 N-linked (GlcNAc...); by host FT (Potential). FT CARBOHYD 18 18 N-linked (GlcNAc...); by host FT (Potential). FT CARBOHYD 22 22 N-linked (GlcNAc...); by host FT (Potential). SQ SEQUENCE 362 AA; 41994 MW; D4C22064001EFECA CRC64; MTTSTNNQTL TQVSNMTNHT LNSTEIYQLF EYTRLGVWLM CIVGTFLNVL VITTILYYRR KKKSPSDTYI CNLAVADLLI VVGLPFFLEY AKHHPKLSRE VVCSGLNACF YICLFAGVCF LINLSMDRYC VIVWGVELNR VRNNKRATCW VVIFWILAVL MGMPHYLMYS HTNNECVGEF ANETSGWFPV FLNTKVNICG YLAPIALMAY TYNRMVRFII NYVGKWHMQT LHVLLVVVVS FASFWFPFNL ALFLESIRLL AGVYNDTLQN VIIFCLYVGQ FLAYVRACLN PGIYILVGTQ MRKDMWTTLR VFACCCVKQE IPYQDIDIEL QKDIQRRAKH TKRTHYDRKN APMESGEEEF LL //