ID ICP27_VZVD Reviewed; 452 AA. AC P09269; DT 01-JUL-1989, integrated into UniProtKB/Swiss-Prot. DT 01-JUL-1989, sequence version 1. DT 11-JAN-2011, entry version 49. DE RecName: Full=Transcriptional transactivator IE4; DE AltName: Full=Immediate-early protein 4; DE Short=IE4; GN ORFNames=ORF4; OS Varicella-zoster virus (strain Dumas) (HHV-3) (Human herpesvirus 3). OC Viruses; dsDNA viruses, no RNA stage; Herpesvirales; Herpesviridae; OC Alphaherpesvirinae; Varicellovirus. OX NCBI_TaxID=10338; OH NCBI_TaxID=9606; Homo sapiens (Human). RN [1] RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. RX MEDLINE=86306657; PubMed=3018124; RA Davison A.J., Scott J.E.; RT "The complete DNA sequence of varicella-zoster virus."; RL J. Gen. Virol. 67:1759-1816(1986). RN [2] RP FUNCTION. RX PubMed=9261438; RA Defechereux P., Debrus S., Baudoux L., Rentier B., Piette J.; RT "Varicella-zoster virus open reading frame 4 encodes an immediate- RT early protein with posttranscriptional regulatory properties."; RL J. Virol. 71:7073-7079(1997). RN [3] RP INTERACTION WITH IE62. RX PubMed=10873781; DOI=10.1006/viro.2000.0389; RA Spengler M.L., Ruyechan W.T., Hay J.; RT "Physical interaction between two varicella zoster virus gene RT regulatory proteins, IE4 and IE62."; RL Virology 272:375-381(2000). RN [4] RP SUBUNIT. RX PubMed=10889190; DOI=10.1074/jbc.M001444200; RA Baudoux L., Defechereux P., Rentier B., Piette J.; RT "Gene activation by Varicella-zoster virus IE4 protein requires its RT dimerization and involves both the arginine-rich sequence, the central RT part, and the carboxyl-terminal cysteine-rich region."; RL J. Biol. Chem. 275:32822-32831(2000). CC -!- FUNCTION: Immediate early (EI) protein that transactivates the CC expression of certain immediate-early, early and late viral genes. CC Acts in concert with IE62 transcriptional transactivator. CC -!- SUBUNIT: Homodimer. Homodimerization is required for CC transactivation. Interacts with IE62; this interaction enhances CC IE62 transactivation. CC -!- SUBCELLULAR LOCATION: Host cytoplasm (By similarity). Host nucleus CC (By similarity). Note=Shuttle between the nucleus and the CC cytoplasm (Probable). IE4 utilizes, at least, XPO1/CRM1 as a CC cofactor for nuclear export. CC -!- SIMILARITY: Belongs to the herpesviridae ICP27 protein family. CC --------------------------------------------------------------------------- CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms CC Distributed under the Creative Commons Attribution (CC BY 4.0) License CC --------------------------------------------------------------------------- DR EMBL; X04370; CAA27887.1; -; Genomic_DNA. DR PIR; D27212; WZBE4. DR ProtClustDB; PHA3118; -. DR GO; GO:0030430; C:host cell cytoplasm; IEA:UniProtKB-SubCell. DR GO; GO:0042025; C:host cell nucleus; IEA:UniProtKB-SubCell. DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW. DR GO; GO:0030528; F:transcription regulator activity; IEA:InterPro. DR InterPro; IPR008648; Herpes_UL69. DR Pfam; PF05459; Herpes_UL69; 1. PE 1: Evidence at protein level; KW Activator; Complete proteome; DNA-binding; Early protein; KW Host cytoplasm; Host nucleus; Transcription; Transcription regulation; KW Virus reference strain. FT CHAIN 1 452 Transcriptional transactivator IE4. FT /FTId=PRO_0000115831. FT COMPBIAS 140 143 Poly-Arg. SQ SEQUENCE 452 AA; 51543 MW; 42926E4A71E380B4 CRC64; MASASIPTDP DVSTICEDFM NLLPDEPSDD FALEVTDWAN DEAIGSTPGE DSTTSRTVYV ERTADTAYNP RYSKRRHGRR ESYHHNRPKT LVVVLPDSNH HGGRDVETGY ARIERGHRRS SRSYNTQSSR KHRDRSLSNR RRRPTTPPAM TTGERNDQTH DESYRLRFSK RDARRERIRK EYDIPVDRIT GRAIEVVSTA GASVTIDSVR HLDETIEKLV VRYATIQEGD SWASGGCFPG IKQNTSWPEL MLYGHELYRT FESYKMDSRI ARALRERVIR GESLIEALES ADELLTWIKM LAAKNLPIYT NNPIVATSKS LLENLKLKLG PFVRCLLLNR DNDLGSRTLP ELLRQQRFSD ITCITTYMFV MIARIANIVV RGSKFVEYDD ISCNVQVLQE YTPGSCLAGV LEALITHQRE CGRVECTLST WAGHLSDARP YGKYFKCSTF NC //