ID CD63_HUMAN Reviewed; 238 AA. AC P08962; DT 01-NOV-1988, integrated into UniProtKB/Swiss-Prot. DT 23-JAN-2007, sequence version 2. DT 25-NOV-2008, entry version 91. DE RecName: Full=CD63 antigen; DE AltName: Full=Melanoma-associated antigen ME491; DE AltName: Full=Ocular melanoma-associated antigen; DE AltName: Full=OMA81H; DE AltName: Full=Granulophysin; DE AltName: Full=Tetraspanin-30; DE Short=Tspan-30; DE AltName: Full=Lysosomal-associated membrane protein 3; DE Short=Lamp-3; DE AltName: CD_antigen=CD63; GN Name=CD63; Synonyms=MLA1, TSPAN30; OS Homo sapiens (Human). OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; OC Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; OC Catarrhini; Hominidae; Homo. OX NCBI_TaxID=9606; RN [1] RP NUCLEOTIDE SEQUENCE [MRNA]. RX MEDLINE=88210273; PubMed=3365686; RA Hotta H., Ross A.H., Huebner K., Isobe M., Wendeborn S., Chao M.V., RA Ricciardi R.P., Tsujimoto Y., Croce C.M., Koprowski H.; RT "Molecular cloning and characterization of an antigen associated with RT early stages of melanoma tumor progression."; RL Cancer Res. 48:2955-2962(1988). RN [2] RP NUCLEOTIDE SEQUENCE [MRNA]. RC TISSUE=Ovary; RX MEDLINE=91025550; PubMed=2171551; RA Rapp G., Freudenstein J., Klaudiny J., Mucha J., Wempe F., Zimmer M., RA Scheit K.H.; RT "Characterization of three abundant mRNAs from human ovarian granulosa RT cells."; RL DNA Cell Biol. 9:479-485(1990). RN [3] RP NUCLEOTIDE SEQUENCE [MRNA], AND SUBCELLULAR LOCATION. RX MEDLINE=91131632; PubMed=1993697; RA Metzelaar M.J., Wigngaard P.L., Peters P.J., Sixma J.J., RA Nieuwenhuis H.K., Clevers H.C.; RT "CD63 antigen. A novel lysosomal membrane glycoprotein, cloned by a RT screening procedure for intracellular antigens in eukaryotic cells."; RL J. Biol. Chem. 266:3239-3245(1991). RN [4] RP NUCLEOTIDE SEQUENCE [MRNA]. RX MEDLINE=92181348; PubMed=1339263; RA Wang M.X., Earley J.J. Jr., Shields J.A., Donoso L.A.; RT "An ocular melanoma-associated antigen. Molecular characterization."; RL Arch. Ophthalmol. 110:399-404(1992). RN [5] RP NUCLEOTIDE SEQUENCE [GENOMIC DNA]. RX MEDLINE=92287132; PubMed=1599482; DOI=10.1016/S0006-291X(05)81004-6; RA Hotta H., Miyamoto H., Hara I., Takahashi N., Homma M.; RT "Genomic structure of the ME491/CD63 antigen gene and functional RT analysis of the 5'-flanking regulatory sequences."; RL Biochem. Biophys. Res. Commun. 185:436-442(1992). RN [6] RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. RA Kalnine N., Chen X., Rolfs A., Halleck A., Hines L., Eisenstein S., RA Koundinya M., Raphael J., Moreira D., Kelley T., LaBaer J., Lin Y., RA Phelan M., Farmer A.; RT "Cloning of human full-length CDSs in BD Creator(TM) system donor RT vector."; RL Submitted (MAY-2003) to the EMBL/GenBank/DDBJ databases. RN [7] RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. RC TISSUE=Lung, and Muscle; RX PubMed=15489334; DOI=10.1101/gr.2596504; RG The MGC Project Team; RT "The status, quality, and expansion of the NIH full-length cDNA RT project: the Mammalian Gene Collection (MGC)."; RL Genome Res. 14:2121-2127(2004). RN [8] RP PROTEIN SEQUENCE OF 2-21. RX MEDLINE=86049405; PubMed=4062294; DOI=10.1016/0003-9861(85)90241-3; RA Ross A.H., Dietzschold B., Jackson D.M., Earley J.J., Ghrist B.F.D., RA Atkinson B., Koprowski H.; RT "Isolation and amino terminal sequencing of a novel melanoma- RT associated antigen."; RL Arch. Biochem. Biophys. 242:540-548(1985). RN [9] RP SUBCELLULAR LOCATION. RX PubMed=10793155; RA Kobayashi T., Vischer U.M., Rosnoblet C., Lebrand C., Lindsay M., RA Parton R.G., Kruithof E.K.O., Gruenberg J.; RT "The tetraspanin CD63/lamp3 cycles between endocytic and secretory RT compartments in human endothelial cells."; RL Mol. Biol. Cell 11:1829-1843(2000). RN [10] RP GLYCOSYLATION AT ASN-130. RX MEDLINE=22660472; PubMed=12754519; DOI=10.1038/nbt827; RA Zhang H., Li X.-J., Martin D.B., Aebersold R.; RT "Identification and quantification of N-linked glycoproteins using RT hydrazide chemistry, stable isotope labeling and mass spectrometry."; RL Nat. Biotechnol. 21:660-666(2003). RN [11] RP SUBCELLULAR LOCATION. RX PubMed=15351990; DOI=10.1002/cyto.a.20068; RA Gruetzkau A., Smorodchenko A., Lippert U., Kirchhof L., Artuc M., RA Henz B.M.; RT "LAMP-1 and LAMP-2, but not LAMP-3, are reliable markers for RT activation-induced secretion of human mast cells."; RL Cytometry 61:62-68(2004). CC -!- FUNCTION: This antigen is associated with early stages of melanoma CC tumor progression. May play a role in growth regulation. CC -!- SUBCELLULAR LOCATION: Cell membrane; Multi-pass membrane protein. CC Lysosome membrane; Multi-pass membrane protein. Late endosome CC membrane; Multi-pass membrane protein. Note=Also found in Weibel- CC Palade bodies of endothelial cells. CC -!- TISSUE SPECIFICITY: Dysplastic nevi, radial growth phase primary CC melanomas, hematopoietic cells, tissue macrophages. CC -!- SIMILARITY: Belongs to the tetraspanin (TM4SF) family. CC --------------------------------------------------------------------------- CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms CC Distributed under the Creative Commons Attribution (CC BY 4.0) License CC --------------------------------------------------------------------------- DR EMBL; X07982; CAA30792.1; -; mRNA. DR EMBL; M59907; AAA63235.1; -; mRNA. DR EMBL; M58485; -; NOT_ANNOTATED_CDS; mRNA. DR EMBL; S93788; AAB21617.1; -; mRNA. DR EMBL; X62654; CAA44519.1; -; Genomic_DNA. DR EMBL; BT007073; AAP35736.1; -; mRNA. DR EMBL; BC002349; AAH02349.1; -; mRNA. DR EMBL; BC013017; AAH13017.1; -; mRNA. DR PIR; I38016; I38016. DR RefSeq; NP_001771.1; -. DR UniGene; Hs.445570; -. DR IntAct; P08962; -. DR Ensembl; ENSG00000135404; Homo sapiens. DR GeneID; 967; -. DR H-InvDB; HIX0010704; -. DR HGNC; HGNC:1692; CD63. DR HPA; CAB000062; -. DR HPA; HPA010088; -. DR MIM; 155740; gene. DR PharmGKB; PA26231; -. DR HOGENOM; P08962; -. DR HOVERGEN; P08962; -. DR Reactome; REACT_604; Hemostasis. DR NextBio; 4044; -. DR CleanEx; HS_CD63; -. DR GermOnline; ENSG00000135404; Homo sapiens. DR GO; GO:0005887; C:integral to plasma membrane; TAS:ProtInc. DR GO; GO:0031902; C:late endosome membrane; IEA:UniProtKB-SubCell. DR GO; GO:0005765; C:lysosomal membrane; TAS:ProtInc. DR InterPro; IPR000301; Transmem_4. DR Pfam; PF00335; Tetraspannin; 1. DR PRINTS; PR00259; TMFOUR. DR PROSITE; PS00421; TM4_1; 1. PE 1: Evidence at protein level; KW Cell membrane; Direct protein sequencing; Endosome; Glycoprotein; KW Lysosome; Membrane; Transmembrane. FT INIT_MET 1 1 Removed. FT CHAIN 2 238 CD63 antigen. FT /FTId=PRO_0000219216. FT TOPO_DOM 2 11 Cytoplasmic (Potential). FT TRANSMEM 12 32 Potential. FT TOPO_DOM 33 51 Extracellular (Potential). FT TRANSMEM 52 72 Potential. FT TOPO_DOM 73 81 Cytoplasmic (Potential). FT TRANSMEM 82 102 Potential. FT TOPO_DOM 103 203 Extracellular (Potential). FT TRANSMEM 204 224 Potential. FT TOPO_DOM 225 238 Cytoplasmic (Potential). FT CARBOHYD 130 130 N-linked (GlcNAc...). FT CARBOHYD 150 150 N-linked (GlcNAc...) (Potential). FT CARBOHYD 172 172 N-linked (GlcNAc...) (Potential). SQ SEQUENCE 238 AA; 25637 MW; 85AC8E235C6E425F CRC64; MAVEGGMKCV KFLLYVLLLA FCACAVGLIA VGVGAQLVLS QTIIQGATPG SLLPVVIIAV GVFLFLVAFV GCCGACKENY CLMITFAIFL SLIMLVEVAA AIAGYVFRDK VMSEFNNNFR QQMENYPKNN HTASILDRMQ ADFKCCGAAN YTDWEKIPSM SKNRVPDSCC INVTVGCGIN FNEKAIHKEG CVEKIGGWLR KNVLVVAAAA LGIAFVEVLG IVFACCLVKS IRSGYEVM //