ID CD63_HUMAN STANDARD; PRT; 237 AA. AC P08962; DT 01-NOV-1988, integrated into UniProtKB/Swiss-Prot. DT 01-NOV-1988, sequence version 1. DT 19-SEP-2006, entry version 69. DE CD63 antigen (Melanoma-associated antigen ME491) (Lysosome-associated DE membrane glycoprotein 3) (LAMP-3) (Ocular melanoma-associated antigen) DE (OMA81H) (Granulophysin) (Tetraspanin-30) (Tspan-30). GN Name=CD63; Synonyms=MLA1, TSPAN30; OS Homo sapiens (Human). OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; OC Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; OC Catarrhini; Hominidae; Homo. OX NCBI_TaxID=9606; RN [1] RP NUCLEOTIDE SEQUENCE [MRNA]. RX MEDLINE=88210273; PubMed=3365686; RA Hotta H., Ross A.H., Huebner K., Isobe M., Wendeborn S., Chao M.V., RA Ricciardi R.P., Tsujimoto Y., Croce C.M., Koprowski H.; RT "Molecular cloning and characterization of an antigen associated with RT early stages of melanoma tumor progression."; RL Cancer Res. 48:2955-2962(1988). RN [2] RP NUCLEOTIDE SEQUENCE [MRNA]. RC TISSUE=Ovary; RX MEDLINE=91025550; PubMed=2171551; RA Rapp G., Freudenstein J., Klaudiny J., Mucha J., Wempe F., Zimmer M., RA Scheit K.H.; RT "Characterization of three abundant mRNAs from human ovarian granulosa RT cells."; RL DNA Cell Biol. 9:479-485(1990). RN [3] RP NUCLEOTIDE SEQUENCE [MRNA]. RX MEDLINE=91131632; PubMed=1993697; RA Metzelaar M.J., Wigngaard P.L., Peters P.J., Sixma J.J., RA Nieuwenhuis H.K., Clevers H.C.; RT "CD63 antigen. A novel lysosomal membrane glycoprotein, cloned by a RT screening procedure for intracellular antigens in eukaryotic cells."; RL J. Biol. Chem. 266:3239-3245(1991). RN [4] RP NUCLEOTIDE SEQUENCE [MRNA]. RX MEDLINE=92181348; PubMed=1339263; RA Wang M.X., Earley J.J. Jr., Shields J.A., Donoso L.A.; RT "An ocular melanoma-associated antigen. Molecular characterization."; RL Arch. Ophthalmol. 110:399-404(1992). RN [5] RP NUCLEOTIDE SEQUENCE [GENOMIC DNA]. RX MEDLINE=92287132; PubMed=1599482; RA Hotta H., Miyamoto H., Hara I., Takahashi N., Homma M.; RT "Genomic structure of the ME491/CD63 antigen gene and functional RT analysis of the 5'-flanking regulatory sequences."; RL Biochem. Biophys. Res. Commun. 185:436-442(1992). RN [6] RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. RA Kalnine N., Chen X., Rolfs A., Halleck A., Hines L., Eisenstein S., RA Koundinya M., Raphael J., Moreira D., Kelley T., LaBaer J., Lin Y., RA Phelan M., Farmer A.; RT "Cloning of human full-length CDSs in BD Creator(TM) system donor RT vector."; RL Submitted (MAY-2003) to the EMBL/GenBank/DDBJ databases. RN [7] RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. RC TISSUE=Lung, and Muscle; RX MEDLINE=22388257; PubMed=12477932; DOI=10.1073/pnas.242603899; RA Strausberg R.L., Feingold E.A., Grouse L.H., Derge J.G., RA Klausner R.D., Collins F.S., Wagner L., Shenmen C.M., Schuler G.D., RA Altschul S.F., Zeeberg B., Buetow K.H., Schaefer C.F., Bhat N.K., RA Hopkins R.F., Jordan H., Moore T., Max S.I., Wang J., Hsieh F., RA Diatchenko L., Marusina K., Farmer A.A., Rubin G.M., Hong L., RA Stapleton M., Soares M.B., Bonaldo M.F., Casavant T.L., Scheetz T.E., RA Brownstein M.J., Usdin T.B., Toshiyuki S., Carninci P., Prange C., RA Raha S.S., Loquellano N.A., Peters G.J., Abramson R.D., Mullahy S.J., RA Bosak S.A., McEwan P.J., McKernan K.J., Malek J.A., Gunaratne P.H., RA Richards S., Worley K.C., Hale S., Garcia A.M., Gay L.J., Hulyk S.W., RA Villalon D.K., Muzny D.M., Sodergren E.J., Lu X., Gibbs R.A., RA Fahey J., Helton E., Ketteman M., Madan A., Rodrigues S., Sanchez A., RA Whiting M., Madan A., Young A.C., Shevchenko Y., Bouffard G.G., RA Blakesley R.W., Touchman J.W., Green E.D., Dickson M.C., RA Rodriguez A.C., Grimwood J., Schmutz J., Myers R.M., RA Butterfield Y.S.N., Krzywinski M.I., Skalska U., Smailus D.E., RA Schnerch A., Schein J.E., Jones S.J.M., Marra M.A.; RT "Generation and initial analysis of more than 15,000 full-length human RT and mouse cDNA sequences."; RL Proc. Natl. Acad. Sci. U.S.A. 99:16899-16903(2002). RN [8] RP PROTEIN SEQUENCE OF 1-20. RX MEDLINE=86049405; PubMed=4062294; RA Ross A.H., Dietzschold B., Jackson D.M., Earley J.J., Ghrist B.F.D., RA Atkinson B., Koprowski H.; RT "Isolation and amino terminal sequencing of a novel melanoma- RT associated antigen."; RL Arch. Biochem. Biophys. 242:540-548(1985). RN [9] RP GLYCOSYLATION AT ASN-129. RX MEDLINE=22660472; PubMed=12754519; DOI=10.1038/nbt827; RA Zhang H., Li X.-J., Martin D.B., Aebersold R.; RT "Identification and quantification of N-linked glycoproteins using RT hydrazide chemistry, stable isotope labeling and mass spectrometry."; RL Nat. Biotechnol. 21:660-666(2003). CC -!- FUNCTION: This antigen is associated with early stages of melanoma CC tumor progression. May play a role in growth regulation. CC -!- SUBCELLULAR LOCATION: Lysosome; lysosomal membrane; multi-pass CC membrane protein. CC -!- TISSUE SPECIFICITY: Dysplastic nevi, radial growth phase primary CC melanomas, hematopoietic cells, tissue macrophages. CC -!- SIMILARITY: Belongs to the tetraspanin (TM4SF) family. CC --------------------------------------------------------------------------- CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms CC Distributed under the Creative Commons Attribution (CC BY 4.0) License CC --------------------------------------------------------------------------- DR EMBL; X07982; CAA30792.1; -; mRNA. DR EMBL; M59907; AAA63235.1; -; mRNA. DR EMBL; M58485; -; NOT_ANNOTATED_CDS; mRNA. DR EMBL; S93788; AAB21617.1; -; mRNA. DR EMBL; X62654; CAA44519.1; -; Genomic_DNA. DR EMBL; BT007073; AAP35736.1; -; mRNA. DR EMBL; BC002349; AAH02349.1; -; mRNA. DR EMBL; BC013017; AAH13017.1; -; mRNA. DR PIR; I38016; I38016. DR UniGene; Hs.445570; -. DR Ensembl; ENSG00000135404; Homo sapiens. DR KEGG; hsa:967; -. DR H-InvDB; HIX0010704; -. DR HGNC; HGNC:1692; CD63. DR MIM; 155740; gene. DR Reactome; P08962; -. DR ArrayExpress; P08962; -. DR RZPD-ProtExp; G0567; -. DR RZPD-ProtExp; IOH3413; -. DR RZPD-ProtExp; RZPDo834C0837; -. DR RZPD-ProtExp; RZPDo839E04121; -. DR GO; GO:0010008; C:endosome membrane; IDA. DR GO; GO:0005887; C:integral to plasma membrane; TAS. DR GO; GO:0005765; C:lysosomal membrane; TAS. DR GO; GO:0005886; C:plasma membrane; TAS. DR InterPro; IPR008952; Tetraspanin. DR InterPro; IPR000301; Transmem_4. DR Pfam; PF00335; Tetraspannin; 1. DR PRINTS; PR00259; TMFOUR. DR PROSITE; PS00421; TM4_1; 1. KW Direct protein sequencing; Glycoprotein; Lysosome; Membrane; KW Transmembrane. FT INIT_MET 0 0 FT CHAIN 1 237 CD63 antigen. FT /FTId=PRO_0000219216. FT TOPO_DOM 1 10 Cytoplasmic (Potential). FT TRANSMEM 11 31 Potential. FT TOPO_DOM 32 50 Extracellular (Potential). FT TRANSMEM 51 71 Potential. FT TOPO_DOM 72 80 Cytoplasmic (Potential). FT TRANSMEM 81 101 Potential. FT TOPO_DOM 102 202 Extracellular (Potential). FT TRANSMEM 203 223 Potential. FT TOPO_DOM 224 237 Cytoplasmic (Potential). FT CARBOHYD 129 129 N-linked (GlcNAc...). FT CARBOHYD 149 149 N-linked (GlcNAc...) (Potential). FT CARBOHYD 171 171 N-linked (GlcNAc...) (Potential). SQ SEQUENCE 237 AA; 25505 MW; 0CBC38F831CF0C9B CRC64; AVEGGMKCVK FLLYVLLLAF CACAVGLIAV GVGAQLVLSQ TIIQGATPGS LLPVVIIAVG VFLFLVAFVG CCGACKENYC LMITFAIFLS LIMLVEVAAA IAGYVFRDKV MSEFNNNFRQ QMENYPKNNH TASILDRMQA DFKCCGAANY TDWEKIPSMS KNRVPDSCCI NVTVGCGINF NEKAIHKEGC VEKIGGWLRK NVLVVAAAAL GIAFVEVLGI VFACCLVKSI RSGYEVM //