ID CISY2_YEAST STANDARD; PRT; 460 AA. AC P08679; DT 01-JAN-1988, integrated into UniProtKB/Swiss-Prot. DT 01-JAN-1988, sequence version 1. DT 07-FEB-2006, entry version 58. DE Citrate synthase, peroxisomal (EC 2.3.3.1). GN Name=CIT2; OrderedLocusNames=YCR005C; ORFNames=YCR5C, YCR043; OS Saccharomyces cerevisiae (Baker's yeast). OC Eukaryota; Fungi; Ascomycota; Saccharomycotina; Saccharomycetes; OC Saccharomycetales; Saccharomycetaceae; Saccharomyces. OX NCBI_TaxID=4932; RN [1] RP NUCLEOTIDE SEQUENCE [GENOMIC DNA]. RX MEDLINE=87089811; PubMed=3540614; RA Rosenkrantz M., Alam T., Kim K.-S., Clark B.J., Srere P.A., RA Guarente L.P.; RT "Mitochondrial and nonmitochondrial citrate synthases in Saccharomyces RT cerevisiae are encoded by distinct homologous genes."; RL Mol. Cell. Biol. 6:4509-4515(1986). RN [2] RP NUCLEOTIDE SEQUENCE [GENOMIC DNA]. RX MEDLINE=92254505; PubMed=1580102; RA Biteau N., Fremaux C., Hebrard S., Menara A., Aigle M., Crouzet M.; RT "The complete sequence of a 10.8kb fragment to the right of the RT chromosome III centromere of Saccharomyces cerevisiae."; RL Yeast 8:61-70(1992). RN [3] RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. RC STRAIN=S288c; RX MEDLINE=92244356; PubMed=1574125; DOI=10.1038/357038a0; RA Oliver S.G., van der Aart Q.J.M., Agostoni-Carbone M.L., Aigle M., RA Alberghina L., Alexandraki D., Antoine G., Anwar R., Ballesta J.P.G., RA Benit P., Berben G., Bergantino E., Biteau N., Bolle P.-A., RA Bolotin-Fukuhara M., Brown A., Brown A.J.P., Buhler J.-M., Carcano C., RA Carignani G., Cederberg H., Chanet R., Contreras R., Crouzet M., RA Daignan-Fornier B., Defoor E., Delgado M.D., Demolder J., Doira C., RA Dubois E., Dujon B., Duesterhoeft A., Erdmann D., Esteban M., RA Fabre F., Fairhead C., Faye G., Feldmann H., Fiers W., RA Francingues-Gaillard M.-C., Franco L., Frontali L., Fukuhara H., RA Fuller L.J., Galland P., Gent M.E., Gigot D., Gilliquet V., RA Glansdorff N., Goffeau A., Grenson M., Grisanti P., Grivell L.A., RA de Haan M., Haasemann M., Hatat D., Hoenicka J., Hegemann J.H., RA Herbert C.J., Hilger F., Hohmann S., Hollenberg C.P., Huse K., RA Iborra F., Indge K.J., Isono K., Jacq C., Jacquet M., James C.M., RA Jauniaux J.-C., Jia Y., Jimenez A., Kelly A., Kleinhans U., Kreisl P., RA Lanfranchi G., Lewis C., van der Linden C.G., Lucchini G., RA Lutzenkirchen K., Maat M.J., Mallet L., Mannhaupt G., Martegani E., RA Mathieu A., Maurer C.T.C., McConnell D., McKee R.A., Messenguy F., RA Mewes H.-W., Molemans F., Montague M.A., Muzi Falconi M., Navas L., RA Newlon C.S., Noone D., Pallier C., Panzeri L., Pearson B.M., Perea J., RA Philippsen P., Pierard A., Planta R.J., Plevani P., Poetsch B., RA Pohl F.M., Purnelle B., Ramezani Rad M., Rasmussen S.W., Raynal A., RA Remacha M.A., Richterich P., Roberts A.B., Rodriguez F., Sanz E., RA Schaaff-Gerstenschlaeger I., Scherens B., Schweitzer B., Shu Y., RA Skala J., Slonimski P.P., Sor F., Soustelle C., Spiegelberg R., RA Stateva L.I., Steensma H.Y., Steiner S., Thierry A., Thireos G., RA Tzermia M., Urrestarazu L.A., Valle G., Vetter I., RA van Vliet-Reedijk J.C., Voet M., Volckaert G., Vreken P., Wang H., RA Warmington J.R., von Wettstein D., Wicksteed B.L., Wilson C., RA Wurst H., Xu G., Yoshikawa A., Zimmermann F.K., Sgouros J.G.; RT "The complete DNA sequence of yeast chromosome III."; RL Nature 357:38-46(1992). RN [4] RP NUCLEOTIDE SEQUENCE [GENOMIC DNA]. RC STRAIN=S288c; RA Marsischky G., Rolfs A., Richardson A., Kane M., Baqui M., Taycher E., RA Hu Y., Vannberg F., Weger J., Kramer J., Moreira D., Kelley F., RA Zuo D., Raphael J., Hogle C., Jepson D., Williamson J., Camargo A., RA Gonzaga L., Vasconcelos A.T., Simpson A., Kolodner R., Harlow E., RA LaBaer J.; RT "Creation of the YFLEX clone resource: cloning of Saccharomyces RT cerevisiae ORFs in the Gateway recombinational cloning system."; RL Submitted (JUL-2004) to the EMBL/GenBank/DDBJ databases. RN [5] RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-24. RX MEDLINE=91094853; PubMed=1986232; RA Liao X., Small W.C., Srere P.A., Butow R.A.; RT "Intramitochondrial functions regulate nonmitochondrial citrate RT synthase (CIT2) expression in Saccharomyces cerevisiae."; RL Mol. Cell. Biol. 11:38-46(1991). RN [6] RP LEVEL OF PROTEIN EXPRESSION. RX MEDLINE=22923965; PubMed=14562106; DOI=10.1038/nature02046; RA Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., RA Dephoure N., O'Shea E.K., Weissman J.S.; RT "Global analysis of protein expression in yeast."; RL Nature 425:737-741(2003). CC -!- CATALYTIC ACTIVITY: Acetyl-CoA + H(2)O + oxaloacetate = citrate + CC CoA. CC -!- PATHWAY: Tricarboxylic acid cycle. CC -!- SUBCELLULAR LOCATION: Peroxisome. CC -!- MISCELLANEOUS: Citrate synthase is found in nearly all cells CC capable of oxidative metabolism. CC -!- MISCELLANEOUS: Present with 2310 molecules/cell. CC -!- SIMILARITY: Belongs to the citrate synthase family. CC --------------------------------------------------------------------------- CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms CC Distributed under the Creative Commons Attribution (CC BY 4.0) License CC --------------------------------------------------------------------------- DR EMBL; Z11113; CAA77442.1; -; Genomic_DNA. DR EMBL; M14686; AAA34497.1; -; Genomic_DNA. DR EMBL; X59720; CAA42342.1; -; Genomic_DNA. DR EMBL; AY692837; AAT92856.1; -; Genomic_DNA. DR EMBL; M54982; AAA34498.1; -; Genomic_DNA. DR PIR; A25393; YKBYC. DR HSSP; P23007; 2CSC. DR GermOnline; 138910; -. DR Ensembl; YCR005C; Saccharomyces cerevisiae. DR SGD; S000000598; CIT2. DR BioCyc; SCER-S28-01:SCER-S28-01-000645-MONOMER; -. DR LinkHub; P08679; -. DR GO; GO:0005739; C:mitochondrion; IDA. DR GO; GO:0005777; C:peroxisome; IDA. DR GO; GO:0004108; F:citrate (Si)-synthase activity; TAS. DR GO; GO:0006101; P:citrate metabolism; TAS. DR GO; GO:0006537; P:glutamate biosynthesis; TAS. DR GO; GO:0006097; P:glyoxylate cycle; TAS. DR InterPro; IPR010109; Cit_synth_euk. DR InterPro; IPR002020; Citrate_synth. DR PANTHER; PTHR11739; Citrate_synth; 1. DR Pfam; PF00285; Citrate_synt; 1. DR PRINTS; PR00143; CITRTSNTHASE. DR TIGRFAMs; TIGR01793; cit_synth_euk; 1. DR PROSITE; PS00480; CITRATE_SYNTHASE; 1. KW Complete proteome; Peroxisome; Transferase; Tricarboxylic acid cycle. FT CHAIN 1 460 Citrate synthase. FT /FTId=PRO_0000169984. FT MOTIF 458 460 Microbody targeting signal (Potential). FT ACT_SITE 293 293 By similarity. FT ACT_SITE 339 339 By similarity. FT ACT_SITE 394 394 By similarity. SQ SEQUENCE 460 AA; 51413 MW; AB2F66AD9A9399EF CRC64; MTVPYLNSNR NVASYLQSNS SQEKTLKERF SEIYPIHAQD VRQFVKEHGK TKISDVLLEQ VYGGMRGIPG SVWEGSVLDP EDGIRFRGRT IADIQKDLPK AKGSSQPLPE ALFWLLLTGE VPTQAQVENL SADLMSRSEL PSHVVQLLDN LPKDLHPMAQ FSIAVTALES ESKFAKAYAQ GISKQDYWSY TFEDSLDLLG KLPVIAAKIY RNVFKDGKMG EVDPNADYAK NLVNLIGSKD EDFVDLMRLY LTIHSDHEGG NVSAHTSHLV GSALSSPYLS LASGLNGLAG PLHGRANQEV LEWLFALKEE VNDDYSKDTI EKYLWDTLNS GRVIPGYGHA VLRKTDPRYM AQRKFAMDHF PDYELFKLVS SIYEVAPGVL TEHGKTKNPW PNVDAHSGVL LQYYGLKESS FYTVLFGVSR AFGILAQLIT DRAIGASIER PKSYSTEKYK ELVKNIESKL //