ID LUCI_PHOPY Reviewed; 550 AA. AC P08659; Q27755; DT 01-JAN-1988, integrated into UniProtKB/Swiss-Prot. DT 01-JAN-1988, sequence version 1. DT 13-OCT-2009, entry version 74. DE RecName: Full=Luciferin 4-monooxygenase; DE Short=Luciferase; DE EC=1.13.12.7; OS Photinus pyralis (North American firefly). OC Eukaryota; Metazoa; Arthropoda; Hexapoda; Insecta; Pterygota; OC Neoptera; Endopterygota; Coleoptera; Polyphaga; Elateriformia; OC Elateroidea; Lampyridae; Lampyrinae; Photinus. OX NCBI_TaxID=7054; RN [1] RP NUCLEOTIDE SEQUENCE [GENOMIC DNA]. RX MEDLINE=87144243; PubMed=3821727; RA de Wet J.R., Wood K.V., Deluca M., Helinski D.R., Subramani S.; RT "Firefly luciferase gene: structure and expression in mammalian RT cells."; RL Mol. Cell. Biol. 7:725-737(1987). RN [2] RP NUCLEOTIDE SEQUENCE [GENOMIC DNA]. RA Croizier G.M.J.; RT "Construction and utilization of an Autographa californica nuclear RT polyhedrosis virus vector with a unique cloning site: expression of RT genes amplified by the polymerase chain reaction."; RL Submitted (JUL-1996) to the EMBL/GenBank/DDBJ databases. RN [3] RP SUBCELLULAR LOCATION. RX MEDLINE=87204117; PubMed=3554235; DOI=10.1073/pnas.84.10.3264; RA Keller G.-A., Gould S., de Luca M., Subramani S.; RT "Firefly luciferase is targeted to peroxisomes in mammalian cells."; RL Proc. Natl. Acad. Sci. U.S.A. 84:3264-3268(1987). RN [4] RP X-RAY CRYSTALLOGRAPHY (2.0 ANGSTROMS). RX MEDLINE=96398615; PubMed=8805533; DOI=10.1016/S0969-2126(96)00033-0; RA Conti E., Franks N.P., Brick P.; RT "Crystal structure of firefly luciferase throws light on a superfamily RT of adenylate-forming enzymes."; RL Structure 4:287-298(1996). RN [5] RP X-RAY CRYSTALLOGRAPHY (2.2 ANGSTROMS). RX MEDLINE=99007339; PubMed=9788915; DOI=10.1016/S0006-3495(98)77664-7; RA Franks N.P., Jenkins A., Conti E., Lieb W.R., Brick P.; RT "Structural basis for the inhibition of firefly luciferase by a RT general anesthetic."; RL Biophys. J. 75:2205-2211(1998). CC -!- FUNCTION: Produces green light with a wavelength of 562 nm. CC -!- CATALYTIC ACTIVITY: Photinus luciferin + O(2) + ATP = oxidized CC Photinus luciferin + CO(2) + AMP + diphosphate + light. CC -!- COFACTOR: Magnesium. CC -!- SUBCELLULAR LOCATION: Peroxisome. CC -!- SIMILARITY: Belongs to the ATP-dependent AMP-binding enzyme CC family. CC -!- WEB RESOURCE: Name=Wikipedia; Note=Firefly luciferase entry; CC URL="http://en.wikipedia.org/wiki/Firefly_luciferase"; CC --------------------------------------------------------------------------- CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms CC Distributed under the Creative Commons Attribution (CC BY 4.0) License CC --------------------------------------------------------------------------- DR EMBL; M15077; AAA29795.1; -; Genomic_DNA. DR EMBL; X84846; CAA59281.1; -; Genomic_DNA. DR EMBL; X84848; CAA59283.1; -; Genomic_DNA. DR EMBL; U03687; AAA03561.1; -; Unassigned_DNA. DR EMBL; U89934; AAB64396.1; -; Genomic_DNA. DR EMBL; U89935; AAB64399.1; -; Genomic_DNA. DR PIR; A26772; A26772. DR PDB; 1BA3; X-ray; 2.20 A; A=1-550. DR PDB; 1LCI; X-ray; 2.00 A; A=1-550. DR PDBsum; 1BA3; -. DR PDBsum; 1LCI; -. DR BRENDA; 1.13.12.7; 142311. DR GO; GO:0005777; C:peroxisome; IDA:UniProtKB. DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW. DR GO; GO:0000287; F:magnesium ion binding; IEA:UniProtKB-KW. DR GO; GO:0047077; F:Photinus-luciferin 4-monooxygenase (ATP-hyd...; IEA:EC. DR GO; GO:0008218; P:bioluminescence; IEA:UniProtKB-KW. DR GO; GO:0055114; P:oxidation reduction; IEA:UniProtKB-KW. DR InterPro; IPR000873; AMP-dep_Synth/Lig. DR Pfam; PF00501; AMP-binding; 1. DR PROSITE; PS00455; AMP_BINDING; 1. PE 1: Evidence at protein level; KW 3D-structure; ATP-binding; Luminescence; Magnesium; Metal-binding; KW Monooxygenase; Nucleotide-binding; Oxidoreductase; Peroxisome; KW Photoprotein. FT CHAIN 1 550 Luciferin 4-monooxygenase. FT /FTId=PRO_0000193147. FT MOTIF 548 550 Microbody targeting signal. FT CONFLICT 361 361 A -> G (in Ref. 2; CAA59281). FT STRAND 7 9 FT HELIX 22 34 FT STRAND 40 44 FT TURN 45 47 FT STRAND 50 52 FT HELIX 53 70 FT STRAND 77 81 FT HELIX 89 98 FT STRAND 101 104 FT HELIX 111 121 FT STRAND 124 128 FT HELIX 130 132 FT HELIX 133 142 FT STRAND 148 153 FT STRAND 161 163 FT HELIX 164 171 FT TURN 178 180 FT TURN 188 190 FT STRAND 191 197 FT STRAND 207 211 FT HELIX 212 222 FT TURN 225 227 FT STRAND 236 239 FT HELIX 246 258 FT STRAND 261 264 FT HELIX 270 279 FT STRAND 283 286 FT HELIX 289 297 FT HELIX 301 303 FT STRAND 311 313 FT HELIX 321 330 FT STRAND 339 341 FT HELIX 343 345 FT STRAND 346 351 FT STRAND 363 365 FT STRAND 370 374 FT TURN 376 378 FT STRAND 388 394 FT STRAND 399 401 FT HELIX 405 411 FT STRAND 418 426 FT STRAND 432 434 FT HELIX 437 439 FT STRAND 442 444 FT STRAND 447 449 FT HELIX 451 459 FT STRAND 464 474 FT TURN 475 477 FT STRAND 478 487 FT HELIX 495 503 FT STRAND 515 518 FT STRAND 528 530 FT HELIX 532 542 SQ SEQUENCE 550 AA; 60745 MW; E380FCE9D56ACCDE CRC64; MEDAKNIKKG PAPFYPLEDG TAGEQLHKAM KRYALVPGTI AFTDAHIEVN ITYAEYFEMS VRLAEAMKRY GLNTNHRIVV CSENSLQFFM PVLGALFIGV AVAPANDIYN ERELLNSMNI SQPTVVFVSK KGLQKILNVQ KKLPIIQKII IMDSKTDYQG FQSMYTFVTS HLPPGFNEYD FVPESFDRDK TIALIMNSSG STGLPKGVAL PHRTACVRFS HARDPIFGNQ IIPDTAILSV VPFHHGFGMF TTLGYLICGF RVVLMYRFEE ELFLRSLQDY KIQSALLVPT LFSFFAKSTL IDKYDLSNLH EIASGGAPLS KEVGEAVAKR FHLPGIRQGY GLTETTSAIL ITPEGDDKPG AVGKVVPFFE AKVVDLDTGK TLGVNQRGEL CVRGPMIMSG YVNNPEATNA LIDKDGWLHS GDIAYWDEDE HFFIVDRLKS LIKYKGYQVA PAELESILLQ HPNIFDAGVA GLPDDDAGEL PAAVVVLEHG KTMTEKEIVD YVASQVTTAK KLRGGVVFVD EVPKGLTGKL DARKIREILI KAKKGGKSKL //