ID LUCI_PHOPY STANDARD; PRT; 550 AA. AC P08659; DT 01-JAN-1988 (REL. 06, CREATED) DT 01-JAN-1988 (REL. 06, LAST SEQUENCE UPDATE) DT 01-JUN-1994 (REL. 29, LAST ANNOTATION UPDATE) DE LUCIFERIN 4-MONOOXYGENASE (EC 1.13.12.7) (LUCIFERASE). OS PHOTINUS PYRALIS (NORTH AMERICAN FIREFLY). OC EUKARYOTA; METAZOA; ARTHROPODA; INSECTA; COLEOPTERA. RN [1] RP SEQUENCE FROM N.A. RM 87144243 RA DE WET J.R., WOOD K.V., DELUCA M., HELINSKI D.R., SUBRAMANI S.; RL MOL. CELL. BIOL. 7:725-737(1987). RN [2] RP SUBCELLULAR LOCATION. RM 87204117 RA KELLER G.-A., GOULD S., DE LUCA M., SUBRAMANI S.; RL PROC. NATL. ACAD. SCI. U.S.A. 84:3264-3268(1987). CC -!- FUNCTION: PRODUCES GREEN LIGHT WITH A WAVELENGTH OF 562 NM. CC -!- CATALYTIC ACTIVITY: LUCIFERIN + O(2) + ATP = OXIDIZED LUCIFERIN + CC CO(2) + H(2)O + AMP + PYROPHOSPHATE + LIGHT. CC -!- COFACTOR: REQUIRE MAGNESIUM. CC -!- SUBCELLULAR LOCATION: PEROXISOMAL. CC -!- SIMILARITY: TO OTHER ENZYMES WHICH ACT VIA AN ATP-DEPENDENT CC COVALENT BINDING OF AMP TO THEIR SUBSTRATE. CC --------------------------------------------------------------------------- CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms CC Distributed under the Creative Commons Attribution (CC BY 4.0) License CC --------------------------------------------------------------------------- DR EMBL; M15077; PPLUC. DR PIR; A26772; A26772. DR PROSITE; PS00342; MICROBODIES_CTER. DR PROSITE; PS00455; AMP_BINDING. KW OXIDOREDUCTASE; MONOOXYGENASE; PHOTOPROTEIN; LUMINESCENCE; MAGNESIUM; KW PEROXISOME. FT SITE 548 550 MICROBODY TARGETING SIGNAL. SQ SEQUENCE 550 AA; 60745 MW; 1596759 CN; MEDAKNIKKG PAPFYPLEDG TAGEQLHKAM KRYALVPGTI AFTDAHIEVN ITYAEYFEMS VRLAEAMKRY GLNTNHRIVV CSENSLQFFM PVLGALFIGV AVAPANDIYN ERELLNSMNI SQPTVVFVSK KGLQKILNVQ KKLPIIQKII IMDSKTDYQG FQSMYTFVTS HLPPGFNEYD FVPESFDRDK TIALIMNSSG STGLPKGVAL PHRTACVRFS HARDPIFGNQ IIPDTAILSV VPFHHGFGMF TTLGYLICGF RVVLMYRFEE ELFLRSLQDY KIQSALLVPT LFSFFAKSTL IDKYDLSNLH EIASGGAPLS KEVGEAVAKR FHLPGIRQGY GLTETTSAIL ITPEGDDKPG AVGKVVPFFE AKVVDLDTGK TLGVNQRGEL CVRGPMIMSG YVNNPEATNA LIDKDGWLHS GDIAYWDEDE HFFIVDRLKS LIKYKGYQVA PAELESILLQ HPNIFDAGVA GLPDDDAGEL PAAVVVLEHG KTMTEKEIVD YVASQVTTAK KLRGGVVFVD EVPKGLTGKL DARKIREILI KAKKGGKSKL //