ID LUCI_PHOPY STANDARD; PRT; 550 AA. AC P08659; DT 01-JAN-1988 (REL. 06, CREATED) DT 01-JAN-1988 (REL. 06, LAST SEQUENCE UPDATE) DT 01-FEB-1991 (REL. 17, LAST ANNOTATION UPDATE) DE LUCIFERIN 4-MONOOXYGENASE (EC 1.13.12.7) (LUCIFERASE). OS PHOTINUS PYRALIS (NORTH AMERICAN FIREFLY). OC EUKARYOTA; METAZOA; ARTHROPODA; INSECTA; COLEOPTERA. RN [1] RP SEQUENCE FROM N.A. RM 87144243 RA DE WET J.R., WOOD K.V., DELUCA M., HELINSKI D.R., SUBRAMANI S.; RL MOL. CELL. BIOL. 7:725-737(1987). RN [2] RP SUBCELLULAR LOCATION. RM 87204117 RA KELLER G.-A., GOULD S., DE LUCA M., SUBRAMANI S.; RL PROC. NATL. ACAD. SCI. U.S.A. 84:3264-3268(1987). CC -!- CATALYTIC ACTIVITY: LUCIFERIN + O(2) + ATP = OXIDIZED LUCIFERIN + CC CO(2) + H(2)O + AMP + PYROPHOSPHATE + LIGHT. CC -!- COFACTOR: REQUIRE MAGNESIUM. CC -!- JAPANESE FIREFLY CATALYSES A REACTION THAT PRODUCES GREEN LIGHT, CC WITH A WAVELENGTH SHORTER (544 NM) THAN THAT EMITTED BY THE NORTH CC AMERICAN FIREFLY (562 NM). THE LUCIFERASES THEMSELVES ARE CC CONSIDERED RESPONSIBLE FOR THE DIFFERENCE IN COLOR OF THE EMITTED CC LIGHTS. CC -!- SUBCELLULAR LOCATION: PEROXISOMES. CC -!- SIMILARITY: 67% OVERALL HOMOLOGY WITH THE JAPANESE FIREFLY CC LUCIFERASE. CC -!- SIMILARITY: TO OTHER ENZYMES WHICH ACT VIA AN ATP-DEPENDENT CC COVALENT BINDING OF AMP TO THEIR SUBSTRATE. CC --------------------------------------------------------------------------- CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms CC Distributed under the Creative Commons Attribution (CC BY 4.0) License CC --------------------------------------------------------------------------- DR EMBL; M15077; PPLUC. DR PIR; A26772; A26772. DR PROSITE; PS00342; MICROBODIES_CTER. DR PROSITE; PS00455; AMP_BINDING. KW OXIDOREDUCTASE; MONOOXYGENASE; PHOTOPROTEIN; LUMINESCENCE; MAGNESIUM; KW PEROXISOME. FT SIMILAR 339 471 TO RAT LIVER LONG-CHAIN-FATTY-ACID-COA FT LIGASE, AA RESIDUES 458-591. FT SITE 548 550 MICROBODY TARGETING SIGNAL. SQ SEQUENCE 550 AA; 60745 MW; 1596759 CN; MEDAKNIKKG PAPFYPLEDG TAGEQLHKAM KRYALVPGTI AFTDAHIEVN ITYAEYFEMS VRLAEAMKRY GLNTNHRIVV CSENSLQFFM PVLGALFIGV AVAPANDIYN ERELLNSMNI SQPTVVFVSK KGLQKILNVQ KKLPIIQKII IMDSKTDYQG FQSMYTFVTS HLPPGFNEYD FVPESFDRDK TIALIMNSSG STGLPKGVAL PHRTACVRFS HARDPIFGNQ IIPDTAILSV VPFHHGFGMF TTLGYLICGF RVVLMYRFEE ELFLRSLQDY KIQSALLVPT LFSFFAKSTL IDKYDLSNLH EIASGGAPLS KEVGEAVAKR FHLPGIRQGY GLTETTSAIL ITPEGDDKPG AVGKVVPFFE AKVVDLDTGK TLGVNQRGEL CVRGPMIMSG YVNNPEATNA LIDKDGWLHS GDIAYWDEDE HFFIVDRLKS LIKYKGYQVA PAELESILLQ HPNIFDAGVA GLPDDDAGEL PAAVVVLEHG KTMTEKEIVD YVASQVTTAK KLRGGVVFVD EVPKGLTGKL DARKIREILI KAKKGGKSKL //