ID PRZN$SERSP STANDARD; PRT; 486 AA. AC P07268; DT 01-APR-1988 (REL. 07, CREATED) DT 01-APR-1988 (REL. 07, LAST SEQUENCE UPDATE) DT 01-FEB-1991 (REL. 17, LAST ANNOTATION UPDATE) DE EXTRACELLULAR METALLOPROTEINASE PRECURSOR (EC 3.4.24.-) (ZINC DE PROTEINASE). OS SERRATIA SP. (STRAIN E-15). OC PROKARYOTA; GRACILICUTES; SCOTOBACTERIA; FACULTATIVELY ANAEROBIC RODS; OC ENTEROBACTERIACEAE. RN [1] RP SEQUENCE FROM N.A. RC MEDLINE=86286592; RA NAKAHAMA K., YOSHIMURA K., MARUMOTO R., KIKUCHI M., LEE I.S., RA HASE T., MATSUBARA H.; RL NUCLEIC ACIDS RES. 14:5843-5855(1986). RN [2] RP SEQUENCE OF 17-34 AND 179-269. RC MEDLINE=85130873; RA LEE I.S., WAKABAYASHI S., MIYATA K., TOMODA K., YONEDA M., KANGAWA K., RA MINAMINO N., MATSUO H., MATSUBARA H.; RL J. BIOCHEM. 96:1409-1418(1984). CC -!- COFACTOR: BINDS AND REQUIRES A ZINC ATOM, WHICH IS ESSENTIAL FOR CC PROTEOLYTIC ACTIVITY. CC -!- THE GLY-RICH REPEATS MAY BE IMPORTANT IN THE EXTRACELLULAR CC SECRETION OF THIS METALLOPROTEASE. CC -!- SIMILARITY: WITH THERMOLYSIN, WITH E.CHRYSANTHEMI PRTB AND PRTD. CC --------------------------------------------------------------------------- CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms CC Distributed under the Creative Commons Attribution (CC BY 4.0) License CC --------------------------------------------------------------------------- DR EMBL; X04127; SSPROT. DR PIR; A23596; A23596. DR PROSITE; PS00142; ZINC_PROTEASE. DR PROSITE; PS00330; HEMOLYSIN_CALCIUM. KW HYDROLASE; ZINC; METALLOPROTEASE; ZYMOGEN; REPEAT. FT PROPEP 1 16 FT CHAIN 17 486 METALLOPROTEINASE. FT METAL 192 192 CATALYTIC ZINC (BY SIMILARITY). FT ACT_SITE 193 193 BY SIMILARITY. FT METAL 196 196 CATALYTIC ZINC (BY SIMILARITY). FT ACT_SITE 245 245 PROTON DONOR (BY SIMILARITY). FT METAL 323 323 CATALYTIC ZINC (BY SIMILARITY). FT DOMAIN 366 389 CONTAINS THREE GLYCINE-RICH REPEATS. FT REPEAT 366 371 FT REPEAT 375 380 FT REPEAT 384 389 SQ SEQUENCE 486 AA; 52406 MW; 1147390 CN; MQSTKKAIEI TESNFAAATT GYDAVDDLLH YHERGNGIQI NGKDSFSNEQ AGLFITRENQ TWNGYKVFGQ PVKLTFSFPD YKFSSTNVAG DTGLSKFSAE QQQQAKLSLQ SWADVANITF TEVAAGQKAN ITFGNYSQDR PGHYDYGTQA YAFLPNTIWQ GQDLGGQTWY NVNQSNVKHP ATEDYGRQTF THEIGHALGL SHPGDYNAGE GNPTYRDVTY AEDTRQFSLM SYWSETNTGG DNGGHYAAAP LLDDIAAIQH LYGANLSTRT GDTVYGFNSN TGRDFLSTTS NSQKVIFAAW DAGGNDTFDF SGYTANQRIN LNEKSFSDVG GLKGNVSIAA GVTIENAIGF RQRLIVGNAA NNVLKGGAGN DVLFGGGGAD ELWGGAGKDI FVFSAASDSA PGASDWIRDF QKGIDKIDLS FFNKEAQSSD FIHFVDHFSG AAGEALLSYN ASNNVTDLSV NIGGHQAPDF LVKIVGQVDV ATDFIV //