ID PUR2_YEAST STANDARD; PRT; 802 AA. AC P07244; DT 01-APR-1988 (Rel. 07, Created) DT 01-APR-1988 (Rel. 07, Last sequence update) DT 05-JUL-2004 (Rel. 44, Last annotation update) DE Bifunctional purine biosynthetic protein ADE5,7 [Includes: DE Phosphoribosylamine--glycine ligase (EC 6.3.4.13) (GARS) (Glycinamide DE ribonucleotide synthetase) (Phosphoribosylglycinamide synthetase); DE Phosphoribosylformylglycinamidine cyclo-ligase (EC 6.3.3.1) (AIRS) DE (Phosphoribosyl-aminoimidazole synthetase) (AIR synthase)]. GN Name=ADE5,7; OrderedLocusNames=YGL234W; OS Saccharomyces cerevisiae (Baker's yeast). OC Eukaryota; Fungi; Ascomycota; Saccharomycotina; Saccharomycetes; OC Saccharomycetales; Saccharomycetaceae; Saccharomyces. OX NCBI_TaxID=4932; RN [1] RP NUCLEOTIDE SEQUENCE. RX MEDLINE=87061006; PubMed=3097325; RA Henikoff S.; RT "The Saccharomyces cerevisiae ADE5,7 protein is homologous to RT overlapping Drosophila melanogaster Gart polypeptides."; RL J. Mol. Biol. 190:519-528(1986). RN [2] RP NUCLEOTIDE SEQUENCE. RA Fartmann B., Kramer B., Kramer W.; RL Submitted (MAY-1996) to the EMBL/GenBank/DDBJ databases. RN [3] RP PARTIAL PROTEIN SEQUENCE OF 1-13. RC STRAIN=S288c; RX MEDLINE=96093904; PubMed=7483834; RA Boucherie H., Dujardin G., Kermorgant M., Monribot C., Slonimski P.P., RA Perrot M.; RT "Two-dimensional protein map of Saccharomyces cerevisiae: construction RT of a gene-protein index."; RL Yeast 11:601-613(1995). CC -!- CATALYTIC ACTIVITY: ATP + 5-phospho-D-ribosylamine + glycine = ADP CC + phosphate + N(1)-(5-phospho-D-ribosyl)glycinamide. CC -!- CATALYTIC ACTIVITY: ATP + 2-(formamido)-N(1)-(5-phospho-D- CC ribosyl)acetamidine = ADP + phosphate + 5-amino-1-(5-phospho-D- CC ribosyl)imidazole. CC -!- PATHWAY: De novo purine biosynthesis; second step. CC -!- PATHWAY: De novo purine biosynthesis; fifth step. CC -!- SIMILARITY: In the N-terminal section; belongs to the GARS family. CC -!- SIMILARITY: In the C-terminal section; belongs to the AIR synthase CC family. CC -!- SIMILARITY: Contains 1 ATP-grasp domain. CC --------------------------------------------------------------------------- CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms CC Distributed under the Creative Commons Attribution (CC BY 4.0) License CC --------------------------------------------------------------------------- DR EMBL; X04337; CAA27867.1; -. DR EMBL; Z72756; CAA96952.1; -. DR PIR; A26343; A26343. DR HSSP; P08178; 1CLI. DR IntAct; P07244; -. DR GermOnline; 141283; -. DR SGD; S000003203; ADE5,7. DR GO; GO:0005737; C:cytoplasm; IDA. DR GO; GO:0006144; P:purine base metabolism; TAS. DR InterPro; IPR000728; AIR_synth. DR InterPro; IPR010918; AIR_synth_C. DR InterPro; IPR000115; Gars. DR InterPro; IPR004733; PurM_cligase. DR InterPro; IPR011054; Rudmnt_hyb_motif. DR Pfam; PF00586; AIRS; 1. DR Pfam; PF02769; AIRS_C; 1. DR Pfam; PF01071; GARS_A; 1. DR Pfam; PF02842; GARS_B; 1. DR Pfam; PF02843; GARS_C; 1. DR Pfam; PF02844; GARS_N; 1. DR TIGRFAMs; TIGR00877; purD; 1. DR TIGRFAMs; TIGR00878; purM; 1. DR PROSITE; PS50975; ATP_GRASP; 1. DR PROSITE; PS00184; GARS; 1. KW Direct protein sequencing; Ligase; Multifunctional enzyme; KW Purine biosynthesis. FT DOMAIN 1 450 GARS. FT DOMAIN 114 330 ATP-grasp. FT DOMAIN 451 802 AIRS. SQ SEQUENCE 802 AA; 86068 MW; 1583C6F3E64085D2 CRC64; MLNILVLGNG AREHVLVTKL AQSPTVGKIY VAPGNGGTAT MDPSRVINWD ITPDVANFAR LQSMAVEHKI NLVVPGPELP LVNGITSVFH SVGIPVFGPS VKAAQLEASK AFSKRFMSKH NIPTASYDVF TNPEEAISFL QAHTDKAFVI KADGIAAGKG VIIPSSIDES VQAIKDIMVT KQFGEEAGKQ VVIEQFLEGD EISLLTIVDG YSHFNLPVAQ DHKRIFDGDK GLNTGGMGAY APAPVATPSL LKTIDSQIVK PTIDGMRRDG MPFVGVLFTG MILVKDSKTN QLVPEVLEYN VRFGDPETQA VLSLLDDQTD LAQVFLAAAE HRLDSVNIGI DDTRSAVTVV VAAGGYPESY AKGDKITLDT DKLPPHTQIF QAGTKYDSAT DSLLTNGGRV LSVTSTAQDL RTAVDTVYEA VKCVHFQNSY YRKDIAYRAF QNSESSKVAI TYADSGVSVD NGNNLVQTIK EMVRSTRRPG ADSDIGGFGG LFDLAQAGFR QNEDTLLVGA TDGVGTKLII AQETGIHNTV GIDLVAMNVN DLVVQGAEPL FFLDYFATGA LDIQVASDFV SGVANGCIQS GCALVGGETS EMPGMYPPGH YDTNGTAVGA VLRQDILPKI NEMAAGDVLL GLASSGVHSN GFSLVRKIIQ HVALPWDAPC PWDESKTLGE GILEPTKIYV KQLLPSIRQR LLLGLAHITG GGLVENIPRA IPDHLQARVD MSTWEVPRVF KWFGQAGNVP HDDILRTFNM GVGMVLIVKR ENVKAVCDSL TEEGEIIWEL GSLQERPKDA PGCVIENGTK LY //