ID PRIB_ECOLI STANDARD; PRT; 103 AA. AC P07013; DT 01-APR-1988 (Rel. 07, Created) DT 01-MAR-1992 (Rel. 21, Last sequence update) DT 10-MAY-2005 (Rel. 47, Last annotation update) DE Primosomal replication protein n. GN Name=priB; OrderedLocusNames=b4201; OS Escherichia coli. OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacteriales; OC Enterobacteriaceae; Escherichia. OX NCBI_TaxID=562; RN [1] RP NUCLEOTIDE SEQUENCE. RX MEDLINE=86310297; PubMed=3528756; DOI=10.1007/BF00330199; RA Schnier J., Kitakawa M., Isono K.; RT "The nucleotide sequence of an Escherichia coli chromosomal region RT containing the genes for ribosomal proteins S6, S18, L9 and an open RT reading frame."; RL Mol. Gen. Genet. 204:126-132(1986). RN [2] RP NUCLEOTIDE SEQUENCE. RC STRAIN=K12 / MG1655; RX MEDLINE=95334362; PubMed=7610040; RA Burland V.D., Plunkett G. III, Sofia H.J., Daniels D.L., RA Blattner F.R.; RT "Analysis of the Escherichia coli genome VI: DNA sequence of the RT region from 92.8 through 100 minutes."; RL Nucleic Acids Res. 23:2105-2119(1995). RN [3] RP PROTEIN SEQUENCE OF 1-27, AND FUNCTION. RC STRAIN=K12; RX MEDLINE=91310687; PubMed=1856227; RA Zavitz K.H., Digate R.J., Marians K.J.; RT "The priB and priC replication proteins of Escherichia coli. Genes, RT DNA sequence, overexpression, and purification."; RL J. Biol. Chem. 266:13988-13995(1991). RN [4] RP PROTEIN SEQUENCE OF 1-27, AND FUNCTION. RX MEDLINE=91268023; PubMed=1646811; RA Allen G.C. Jr., Kornberg A.; RT "The priB gene encoding the primosomal replication n protein of RT Escherichia coli."; RL J. Biol. Chem. 266:11610-11613(1991). RN [5] RP FUNCTION. RX MEDLINE=93374896; PubMed=8366072; RA Allen G.C. Jr., Kornberg A.; RT "Assembly of the primosome of DNA replication in Escherichia coli."; RL J. Biol. Chem. 268:19204-19209(1993). RN [6] RP SUBUNIT. RX MEDLINE=96279088; PubMed=8663106; DOI=10.1074/jbc.271.26.15656; RA Liu J., Nurse P., Marians K.J.; RT "The ordered assembly of the phiX174-type primosome. III. PriB RT facilitates complex formation between PriA and DnaT."; RL J. Biol. Chem. 271:15656-15661(1996). CC -!- FUNCTION: Binds single-stranded DNA at the primosome assembly site CC (PAS). During primosome assembly it facilitates the complex CC formation between priA and dnaT. CC -!- SUBUNIT: Component of the preprimosomal complex composed of one CC monomer of priC and dnaT, two monomers of priA, two dimers of priB CC and one hexamer of dnaB. Upon transient interaction with dnaG it CC forms the primosome. CC -!- SIMILARITY: Belongs to the priB family. CC -!- SIMILARITY: Contains 1 SSB domain. CC --------------------------------------------------------------------------- CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms CC Distributed under the Creative Commons Attribution (CC BY 4.0) License CC --------------------------------------------------------------------------- DR EMBL; X04022; CAA27653.1; -; Genomic_DNA. DR EMBL; U14003; AAA97097.1; -; Genomic_DNA. DR EMBL; U00096; AAC77158.1; -; Genomic_DNA. DR PIR; A30281; Q4ECFR. DR PDB; 1TXY; X-ray; A/B=1-103. DR PDB; 1V1Q; X-ray; A/B=1-101. DR PDB; 1WOC; X-ray; A/B/C/D=1-103. DR EchoBASE; EB0757; -. DR EcoGene; EG10764; priB. DR HAMAP; MF_00720; -; 1. DR InterPro; IPR012340; NA-bind_OB_sub. DR InterPro; IPR000424; SSB/primosomal_n. DR InterPro; IPR010913; ssDNA_binding. DR Pfam; PF00436; SSB; 1. DR PROSITE; PS50935; SSB; 1. KW 3D-structure; Complete proteome; Direct protein sequencing; KW DNA replication; DNA-binding; Primosome. FT INIT_MET 0 0 FT DOMAIN 1 100 SSB. SQ SEQUENCE 103 AA; 11311 MW; 98A136EA72061DCC CRC64; TNRLVLSGTV CRAPLRKVSP SGIPHCQFVL EHRSVQEEAG FHRQAWCQMP VIVSGHENQA ITHSITVGSR ITVQGFISCH KAKNGLSKMV LHAEQIELID SGD //