ID PRIB_ECOLI STANDARD; PRT; 103 AA. AC P07013; DT 01-APR-1988 (Rel. 07, Created) DT 01-MAR-1992 (Rel. 21, Last sequence update) DT 15-JUN-2004 (Rel. 44, Last annotation update) DE Primosomal replication protein n. GN PRIB OR B4201. OS Escherichia coli. OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacteriales; OC Enterobacteriaceae; Escherichia. OX NCBI_TaxID=562; RN [1] RP SEQUENCE FROM N.A. RX MEDLINE=86310297; PubMed=3528756; RA Schnier J., Kitakawa M., Isono K.; RT "The nucleotide sequence of an Escherichia coli chromosomal region RT containing the genes for ribosomal proteins S6, S18, L9 and an open RT reading frame."; RL Mol. Gen. Genet. 204:126-132(1986). RN [2] RP SEQUENCE FROM N.A. RC STRAIN=K12 / MG1655; RX MEDLINE=95334362; PubMed=7610040; RA Burland V.D., Plunkett G. III, Sofia H.J., Daniels D.L., RA Blattner F.R.; RT "Analysis of the Escherichia coli genome VI: DNA sequence of the RT region from 92.8 through 100 minutes."; RL Nucleic Acids Res. 23:2105-2119(1995). RN [3] RP SEQUENCE OF 1-27, AND FUNCTION. RC STRAIN=K12; RX MEDLINE=91310687; PubMed=1856227; RA Zavitz K.H., Digate R.J., Marians K.J.; RT "The priB and priC replication proteins of Escherichia coli. Genes, RT DNA sequence, overexpression, and purification."; RL J. Biol. Chem. 266:13988-13995(1991). RN [4] RP SEQUENCE OF 1-27, AND FUNCTION. RX MEDLINE=91268023; PubMed=1646811; RA Allen G.C. Jr., Kornberg A.; RT "The priB gene encoding the primosomal replication n protein of RT Escherichia coli."; RL J. Biol. Chem. 266:11610-11613(1991). RN [5] RP FUNCTION. RX MEDLINE=93374896; PubMed=8366072; RA Allen G.C. Jr., Kornberg A.; RT "Assembly of the primosome of DNA replication in Escherichia coli."; RL J. Biol. Chem. 268:19204-19209(1993). RN [6] RP SUBUNIT. RX MEDLINE=96279088; PubMed=8663106; RA Liu J., Nurse P., Marians K.J.; RT "The ordered assembly of the phiX174-type primosome. III. PriB RT facilitates complex formation between PriA and DnaT."; RL J. Biol. Chem. 271:15656-15661(1996). CC -!- FUNCTION: Binds single-stranded DNA at the primosome assembly site CC (PAS). During primosome assembly it facilitates the complex CC formation between priA and dnaT. CC -!- SUBUNIT: Component of the preprimosomal complex composed of one CC monomer of priC and dnaT, two monomers of priA, two dimers of CC priB and one hexamer of dnaB. Upon transient interaction with CC dnaG it forms the primosome. CC -!- SIMILARITY: Belongs to the priB family. CC -!- SIMILARITY: Contains 1 SSB domain. CC --------------------------------------------------------------------------- CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms CC Distributed under the Creative Commons Attribution (CC BY 4.0) License CC --------------------------------------------------------------------------- DR EMBL; X04022; CAA27653.1; -. DR EMBL; U14003; AAA97097.1; -. DR EMBL; AE000491; AAC77158.1; -. DR PIR; A30281; Q4ECFR. DR EcoGene; EG10764; priB. DR HAMAP; MF_00720; -; 1. DR InterPro; IPR008994; Nucleic_acid_OB. DR InterPro; IPR000424; SSB_protein. DR Pfam; PF00436; SSB; 1. DR PROSITE; PS50935; SSB; 1. KW DNA replication; Primosome; DNA-binding; Complete proteome; KW Direct protein sequencing. FT INIT_MET 0 0 FT DOMAIN 1 100 SSB. SQ SEQUENCE 103 AA; 11311 MW; 98A136EA72061DCC CRC64; TNRLVLSGTV CRAPLRKVSP SGIPHCQFVL EHRSVQEEAG FHRQAWCQMP VIVSGHENQA ITHSITVGSR ITVQGFISCH KAKNGLSKMV LHAEQIELID SGD //