ID DPOL_VACCW Reviewed; 1006 AA. AC P06856; Q76ZV6; DT 01-JAN-1988, integrated into UniProtKB/Swiss-Prot. DT 07-DEC-2004, sequence version 4. DT 08-MAR-2011, entry version 72. DE RecName: Full=DNA polymerase; DE EC=2.7.7.7; GN Name=POL; OrderedLocusNames=VACWR065; ORFNames=E9L; OS Vaccinia virus (strain Western Reserve) (VACV) (Vaccinia virus (strain OS WR)). OC Viruses; dsDNA viruses, no RNA stage; Poxviridae; Chordopoxvirinae; OC Orthopoxvirus; Vaccinia virus. OX NCBI_TaxID=10254; OH NCBI_TaxID=9913; Bos taurus (Bovine). RN [1] RP NUCLEOTIDE SEQUENCE [GENOMIC DNA]. RA Gershon P.D., Jones E.V., Moss B., Ahn B.-Y.; RL Submitted (JUL-1990) to the EMBL/GenBank/DDBJ databases. RN [2] RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. RA Esposito J.J., Frace A.M., Sammons S.A., Olsen-Rasmussen M., RA Osborne J., Wohlhueter R.; RT "Sequencing of the coding region of Vaccinia-WR to an average 9-fold RT redundancy and an error rate of 0.16/10kb."; RL Submitted (FEB-2003) to the EMBL/GenBank/DDBJ databases. RN [3] RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 40-962. RX MEDLINE=86233293; PubMed=3012524; DOI=10.1073/pnas.83.11.3659; RA Earl P.L., Jones E.V., Moss B.; RT "Homology between DNA polymerases of poxviruses, herpesviruses, and RT adenoviruses: nucleotide sequence of the vaccinia virus DNA polymerase RT gene."; RL Proc. Natl. Acad. Sci. U.S.A. 83:3659-3663(1986). RN [4] RP MUTANTS TS42; NG26 AND PAARVG. RX MEDLINE=89095014; PubMed=2911123; RA Traktman P., Kelvin M., Pacheco S.; RT "Molecular genetic analysis of vaccinia virus DNA polymerase RT mutants."; RL J. Virol. 63:841-846(1989). CC -!- CATALYTIC ACTIVITY: Deoxynucleoside triphosphate + DNA(n) = CC diphosphate + DNA(n+1). CC -!- MISCELLANEOUS: Ts42 is a temperature-sensitive mutant. PAArVg is a CC mutant resistant to the drug phosphonoacetic acid (PAA). NG26 is a CC double mutant with a temperature-sensitive lesion and a mutation CC rendering the virus resistant to the drug phosphonoacetic acid CC (PAA). CC -!- SIMILARITY: Belongs to the DNA polymerase type-B family. CC -!- SEQUENCE CAUTION: CC Sequence=AAA98419.1; Type=Erroneous initiation; CC --------------------------------------------------------------------------- CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms CC Distributed under the Creative Commons Attribution (CC BY 4.0) License CC --------------------------------------------------------------------------- DR EMBL; M36339; AAB59829.1; -; Genomic_DNA. DR EMBL; AY243312; AAO89344.1; -; Genomic_DNA. DR EMBL; M13213; AAA98419.1; ALT_INIT; Genomic_DNA. DR PIR; A24878; A24878. DR PIR; A25270; DJVZZW. DR RefSeq; YP_232947.1; NC_006998.1. DR ProteinModelPortal; P06856; -. DR MINT; MINT-6740349; -. DR GeneID; 3707598; -. DR ProtClustDB; PHA3036; -. DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW. DR GO; GO:0003887; F:DNA-directed DNA polymerase activity; IEA:UniProtKB-KW. DR GO; GO:0000166; F:nucleotide binding; IEA:InterPro. DR GO; GO:0006260; P:DNA replication; IEA:UniProtKB-KW. DR InterPro; IPR006172; DNA-dir_DNA_pol_B. DR InterPro; IPR017964; DNA-dir_DNA_pol_B_CS. DR InterPro; IPR006133; DNA-dir_DNA_pol_B_exonuc. DR InterPro; IPR006134; DNA-dir_DNA_pol_B_multi_dom. DR InterPro; IPR013617; DNA-dir_DNA_pol_B_vir_insert. DR InterPro; IPR023211; DNA_pol_palm_dom. DR InterPro; IPR013660; DNApol_B_exo_N. DR InterPro; IPR012337; RNaseH-like. DR Gene3D; G3DSA:3.90.1600.10; DNA_pol_palm_dom; 3. DR Pfam; PF00136; DNA_pol_B; 1. DR Pfam; PF08408; DNA_pol_B_3; 1. DR Pfam; PF03104; DNA_pol_B_exo; 1. DR Pfam; PF08452; DNAP_B_exo_N; 1. DR PRINTS; PR00106; DNAPOLB. DR SMART; SM00486; POLBc; 1. DR SUPFAM; SSF53098; RNaseH_fold; 1. DR PROSITE; PS00116; DNA_POLYMERASE_B; 1. PE 3: Inferred from homology; KW Complete proteome; DNA replication; DNA-binding; KW DNA-directed DNA polymerase; Nucleotidyltransferase; Transferase; KW Virus reference strain. FT CHAIN 1 1006 DNA polymerase. FT /FTId=PRO_0000046533. FT VARIANT 338 338 S -> F (in PAA-R mutant; FT phosphonoacetate-resistant). FT VARIANT 372 372 G -> D (in PAA-R, NG26 and PAArVg FT mutants; phosphonoacetate-resistant). FT VARIANT 392 392 G -> D (in NG26 mutant; temperature- FT sensitivity). FT VARIANT 611 611 E -> K (in ts42 mutant; temperature- FT sensitivity). FT CONFLICT 296 296 H -> Y (in Ref. 1 and 3). SQ SEQUENCE 1006 AA; 116903 MW; 9D68FE95A93AE536 CRC64; MDVRCINWFE SHGENRFLYL KSRCRNGETV FIRFPHYFYY VVTDEIYQSL SPPPFNARPL GKMRTIDIDE TISYNLDIKD RKCSVADMWL IEEPKKRSIQ NATMDEFLNI SWFYISNGIS PDGCYSLDEQ YLTKINNGCY HCDDPRNCFA KKIPRFDIPR SYLFLDIECH FDKKFPSVFI NPISHTSYCY IDLSGKRLLF TLINEEMLTE QEIQEAVDRG CLRIQSLMEM DYERELVLCS EIVLLRIAKQ LLELTFDYVV TFNGHNFDLR YITNRLELLT GEKIIFRSPD KKEAVHLCIY ERNQSSHKGV GGMANTTFHV NNNNGTIFFD LYSFIQKSEK LDSYKLDSIS KNAFSCMGKV LNRGVREMTF IGDDTTDAKG KAAAFAKVLT TGNYVTVDED IICKVIRKDI WENGFKVVLL CPTLPNDTYK LSFGKDDVDL AQMYKDYNLN IALDMARYCI HDACLCQYLW EYYGVETKTD AGASTYVLPQ SMVFEYRAST VIKGPLLKLL LETKTILVRS ETKQKFPYEG GKVFAPKQKM FSNNVLIFDY NSLYPNVCIF GNLSPETLVG VVVSTNRLEE EINNQLLLQK YPPPRYITVH CEPRLPNLIS EIAIFDRSIE GTIPRLLRTF LAERARYKKM LKQATSSTEK AIYDSMQYTY KIVANSVYGL MGFRNSALYS YASAKSCTSI GRRMILYLES VLNGAELSNG MLRFANPLSN PFYMDDRDIN PIVKTSLPID YRFRFRSVYG DTDSVFTEID SQDVDKSIEI AKELERLINN RVLFNNFKIE FEAVYKNLIM QSKKKYTTMK YSASSNSKSV PERINKGTSE TRRDVSKFHK NMIKTYKTRL SEMLSEGRMN SNQVCIDILR SLETDLRSEF DSRSSPLELF MLSRMHHSNY KSADNPNMYL VTEYNKNNPE TIELGERYYF AYICPANVPW TKKLVNIKTY ETIIDRSFKL GSDQRIFYEV YFKRLTSEIV NLLDNKVLCI SFFERMFGSK PTFYEA //