ID TOP1_ECOLI STANDARD; PRT; 864 AA. AC P06612; DT 01-JAN-1988 (REL. 06, CREATED) DT 01-APR-1988 (REL. 07, LAST SEQUENCE UPDATE) DT 01-DEC-1992 (REL. 24, LAST ANNOTATION UPDATE) DE DNA TOPOISOMERASE I (EC 5.99.1.2) (OMEGA-PROTEIN) (RELAXING ENZYME) DE (UNTWISTING ENZYME) (SWIVELASE). GN TOPA OR SUPX. OS ESCHERICHIA COLI. OC PROKARYOTA; GRACILICUTES; SCOTOBACTERIA; FACULTATIVELY ANAEROBIC RODS; OC ENTEROBACTERIACEAE. RN [1] RP SEQUENCE FROM N.A. RM 87141163 RA TSE-DINH Y.-C., WANG J.C.; RL J. MOL. BIOL. 191:321-331(1986). RN [2] RP SEQUENCE OF 697-864 FROM N.A. RM 87194810 RA OSTROWSKI J., JAGURA-BURDZY G., KREDICH N.M.; RL J. BIOL. CHEM. 262:5999-6005(1987). CC -!- FUNCTION: THE REACTION CATALYZED BY TOPOISOMERASES LEADS TO THE CC CONVERSION OF ONE TOPOLOGICAL ISOMER OF DNA TO ANOTHER. CC -!- CATALYTIC ACTIVITY: ATP-INDEPENDENT BREAKAGE OF SINGLE-STRANDED CC DNA, FOLLOWED BY PASSAGE AND REJOINING. CC -!- WHEN A TOPOISOMERASE TRANSIENTLY BREAKS A DNA BACKBONE BOND, IT CC SIMULTANEOUSLY FORMS A PROTEIN-DNA LINK, IN WHICH A TYROSYL OXYGEN CC IN THE ENZYME IS JOINED TO A DNA PHOSPHORUS AT ONE END OF THE CC ENZYME-SEVERED DNA STRAND. CC -!- SUBUNIT: MONOMER. CC -!- SIMILARITY: STRONG TO E.COLI AND YEAST TOPOISOMERASES III. CC --------------------------------------------------------------------------- CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms CC Distributed under the Creative Commons Attribution (CC BY 4.0) License CC --------------------------------------------------------------------------- DR EMBL; X04475; ECTOPA. DR EMBL; M15041; ECCYSB. DR PIR; A25786; ISECTP. DR ECO2DBASE; I115.0; 5TH EDITION. DR ECOGENE; EG11013; TOPA. DR PROSITE; PS00396; TOPOISOMERASE_I_PROK. KW ISOMERASE; TOPOISOMERASE; DNA-BINDING. FT ACT_SITE 319 319 DNA CLEAVAGE. FT CONFLICT 786 786 P -> R (IN REF. 2). SQ SEQUENCE 864 AA; 97402 MW; 3718068 CN; MGKALVIVES PAKAKTINKY LGSDYVVKSS VGHIRDLPTS GSAAKKSADS TSTKTAKKPK KDERGALVNR MGVDPWHNWE AHYEVLPGKE KVVSELKQLA EKADHIYLAT DLDREGEAIA WRLREVIGGD DARYSRVVFN EIAKNAIRQA FNKPGELNID RVNAQRARRF MDRVVGYMVS PLLWKKIARG LSAGRVQSVA VRLAVERERE IKAFVPEEFW EVDASTTTPS GEALALQVTH QNDKPFRPVN KEQTQAAVSL LEKARYSVLE REDKPTTSKP SAPLITSTLQ QAASTRLGFG VKKTMMMAQR LYEAGYITYM RTDSTNLSQD AVNMVRGYIS DNFGKKYLPE SPNRYASKEN SQEAHEAIRP SDVNVMAESL KDMEADAQKL YQLIWRQFVA CQMTPAKYDS TTLTVGAGDF RLKARGRILR FDGWTKVMPA LRKGDEDRIL PAVNKGDALT LVELTPAQHF TKPPARFSEA SLVKELEKRG IGRPSTYASI ISTIQDRGYV RVENRRFYAE KMGEIVTDRL EENFRELMNY DFTAQMENSL DQVANHEAEW KAVLDHFFSD FTQQLVSRKV RKRVVCARTR MVLTSIDCPT CGRKMGIRTA STGVFLGCSG YALPPKERCK TTINVVPENE VLNVLEGEDA ETKPLRAKRR CPKCGTAMDS YLIDPKRKLH VCGNNPTCDG YEIEEGEFRI KGYDGPIVEC EKCGSEMHLK MGRFGKYMAC TNEECKNTRK ILRNGEVAPP KEDPVPLPEL PCEKSDAYFV LRDGAAGVFL AANTFPKSRE TRAPLVEELY RFRDRLPEKL RYLADAPQQD PEGNKTMVRF SRKTKQQYVS SEKDGKATGW SAFYVDGKWV EGKK //