ID TOP1$ECOLI STANDARD; PRT; 864 AA. AC P06612; DT 01-NOV-1988 (ANNOTATIONS EDITED) DT 01-APR-1988 (SEQUENCE AND ANNOTATIONS EDITED) DT 01-JAN-1988 (TRANSLATED FROM AN EMBL ENTRY) DE DNA TOPOISOMERASE I (EC 5.99.1.2) (OMEGA-PROTEIN) (RELAXING ENZYME) DE (UNTWISTING ENZYME) (SWIVELASE) (GENE NAME: TOPA). OS ESCHERICHIA COLI. OC PROKARYOTA. RN [1] (SEQUENCE FROM N.A.) RA TSE-DINH Y.-C., WANG J.C.; RL J. MOL. BIOL. 191:321-331(1986). RN [2] (SEQUENCE OF 697-864 FROM N.A.) RA OSTROWSKI J., JAGURA-BURDZY G., KREDICH N.M.; RL J. BIOL. CHEM. 262:5999-6005(1987). CC -!- FUNCTION: TYPE I DNA TOPOISOMERASE CATALYZES THE ATP-INDEPENDENT CC TRANSIENT BREAKAGE OF DNA PHOSPHODIESTER BONDS (ONE STRAND AT A CC TIME), AND THE SUBSEQUENT PASSAGE OF A SINGLE STRAND ACROSS THE CC BREAK IN ANOTHER, FOLLOWED BY REJOINING. THIS REACTION WILL LEAD CC TO THE CONVERSION OF ONE TOPOLOGICAL ISOMER OF DNA TO ANOTHER. CC -!- SUBUNIT: MONOMER. CC --------------------------------------------------------------------------- CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms CC Distributed under the Creative Commons Attribution (CC BY 4.0) License CC --------------------------------------------------------------------------- DR EMBL; X04475; ECTOPA. DR EMBL; M15041; ECCYSB. DR PIR; A25786; ISECTP. KW ISOMERASE; TOPOISOMERASE; DNA-BINDING. FT CONFLICT 786 786 P -> R (IN REF. 2). SQ SEQUENCE 864 AA; 97401 MW; 3718068 CN; MGKALVIVES PAKAKTINKY LGSDYVVKSS VGHIRDLPTS GSAAKKSADS TSTKTAKKPK KDERGALVNR MGVDPWHNWE AHYEVLPGKE KVVSELKQLA EKADHIYLAT DLDREGEAIA WRLREVIGGD DARYSRVVFN EIAKNAIRQA FNKPGELNID RVNAQRARRF MDRVVGYMVS PLLWKKIARG LSAGRVQSVA VRLAVERERE IKAFVPEEFW EVDASTTTPS GEALALQVTH QNDKPFRPVN KEQTQAAVSL LEKARYSVLE REDKPTTSKP SAPLITSTLQ QAASTRLGFG VKKTMMMAQR LYEAGYITYM RTDSTNLSQD AVNMVRGYIS DNFGKKYLPE SPNRYASKEN SQEAHEAIRP SDVNVMAESL KDMEADAQKL YQLIWRQFVA CQMTPAKYDS TTLTVGAGDF RLKARGRILR FDGWTKVMPA LRKGDEDRIL PAVNKGDALT LVELTPAQHF TKPPARFSEA SLVKELEKRG IGRPSTYASI ISTIQDRGYV RVENRRFYAE KMGEIVTDRL EENFRELMNY DFTAQMENSL DQVANHEAEW KAVLDHFFSD FTQQLVSRKV RKRVVCARTR MVLTSIDCPT CGRKMGIRTA STGVFLGCSG YALPPKERCK TTINVVPENE VLNVLEGEDA ETKPLRAKRR CPKCGTAMDS YLIDPKRKLH VCGNNPTCDG YEIEEGEFRI KGYDGPIVEC EKCGSEMHLK MGRFGKYMAC TNEECKNTRK ILRNGEVAPP KEDPVPLPEL PCEKSDAYFV LRDGAAGVFL AANTFPKSRE TRAPLVEELY RFRDRLPEKL RYLADAPQQD PEGNKTMVRF SRKTKQQYVS SEKDGKATGW SAFYVDGKWV EGKK //