ID RL2_YEAST Reviewed; 254 AA. AC P05736; DT 01-NOV-1988, integrated into UniProtKB/Swiss-Prot. DT 23-JAN-2007, sequence version 3. DT 22-JUL-2008, entry version 89. DE RecName: Full=60S ribosomal protein L2; DE AltName: Full=L5; DE AltName: Full=YL6; DE AltName: Full=RP8; GN Name=RPL2A; Synonyms=RPL5B; OrderedLocusNames=YFR031C-A; GN ORFNames=YFR031BC; GN and GN Name=RPL2B; Synonyms=RPL5A; OrderedLocusNames=YIL018W; OS Saccharomyces cerevisiae (Baker's yeast). OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; OC Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces. OX NCBI_TaxID=4932; RN [1] RP NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA] (RPL2A AND RPL2B). RC STRAIN=ATCC 204660 / DBY746; RX MEDLINE=95272535; PubMed=7753035; DOI=10.1007/BF00705656; RA Moore J., Jacobs H.T., Kaiser K.; RT "Characterisation of Saccharomyces cerevisiae genes encoding ribosomal RT protein YL6."; RL Mol. Gen. Genet. 247:247-254(1995). RN [2] RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] (RPL2A). RC STRAIN=ATCC 204511 / S288c / AB972; RX MEDLINE=96287654; PubMed=8686381; RX DOI=10.1002/(SICI)1097-0061(199602)12:2<177::AID-YEA896>3.3.CO;2-1; RA Eki T., Naitou M., Hagiwara H., Abe M., Ozawa M., Sasanuma S., RA Sasanuma M., Tsuchiya Y., Shibata T., Watanabe K., Ono A., RA Yamazaki M., Tashiro H., Hanaoka F., Murakami Y.; RT "Fifteen open reading frames in a 30.8 kb region of the right arm of RT chromosome VI from Saccharomyces cerevisiae."; RL Yeast 12:177-190(1996). RN [3] RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA] (RPL2A). RC STRAIN=ATCC 204511 / S288c / AB972; RX MEDLINE=95400292; PubMed=7670463; DOI=10.1038/ng0795-261; RA Murakami Y., Naitou M., Hagiwara H., Shibata T., Ozawa M., RA Sasanuma S., Sasanuma M., Tsuchiya Y., Soeda E., Yokoyama K., RA Yamazaki M., Tashiro H., Eki T.; RT "Analysis of the nucleotide sequence of chromosome VI from RT Saccharomyces cerevisiae."; RL Nat. Genet. 10:261-268(1995). RN [4] RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA] (RPL2B). RC STRAIN=ATCC 204511 / S288c / AB972; RX MEDLINE=97313266; PubMed=9169870; RA Churcher C.M., Bowman S., Badcock K., Bankier A.T., Brown D., RA Chillingworth T., Connor R., Devlin K., Gentles S., Hamlin N., RA Harris D.E., Horsnell T., Hunt S., Jagels K., Jones M., Lye G., RA Moule S., Odell C., Pearson D., Rajandream M.A., Rice P., Rowley N., RA Skelton J., Smith V., Walsh S.V., Whitehead S., Barrell B.G.; RT "The nucleotide sequence of Saccharomyces cerevisiae chromosome IX."; RL Nature 387:84-87(1997). RN [5] RP PROTEIN SEQUENCE OF 2-41. RA Otaka E., Higo K., Itoh T.; RT "Yeast ribosomal proteins. VIII. Isolation of two proteins and RT sequence characterization of twenty-four proteins from cytoplasmic RT ribosomes."; RL Mol. Gen. Genet. 195:544-546(1984). RN [6] RP PROTEIN SEQUENCE OF 2-21. RX MEDLINE=92184799; PubMed=1544921; RA Takakura H., Tsunasawa S., Miyagi M., Warner J.R.; RT "NH2-terminal acetylation of ribosomal proteins of Saccharomyces RT cerevisiae."; RL J. Biol. Chem. 267:5442-5445(1992). RN [7] RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 226-254 (RPL2A). RX MEDLINE=95212908; PubMed=7698648; RA Strunnikov A.V., Hogan E., Koshland D.; RT "SMC2, a Saccharomyces cerevisiae gene essential for chromosome RT segregation and condensation, defines a subgroup within the SMC RT family."; RL Genes Dev. 9:587-599(1995). RN [8] RP NOMENCLATURE, AND SUBUNIT. RX PubMed=9559554; RX DOI=10.1002/(SICI)1097-0061(19980330)14:5<471::AID-YEA241>3.0.CO;2-U; RA Planta R.J., Mager W.H.; RT "The list of cytoplasmic ribosomal proteins of Saccharomyces RT cerevisiae."; RL Yeast 14:471-477(1998). RN [9] RP SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS]. RX MEDLINE=22923954; PubMed=14562095; DOI=10.1038/nature02026; RA Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., RA Weissman J.S., O'Shea E.K.; RT "Global analysis of protein localization in budding yeast."; RL Nature 425:686-691(2003). RN [10] RP LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS]. RX MEDLINE=22923965; PubMed=14562106; DOI=10.1038/nature02046; RA Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., RA Dephoure N., O'Shea E.K., Weissman J.S.; RT "Global analysis of protein expression in yeast."; RL Nature 425:737-741(2003). RN [11] RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-20; SER-52; THR-84; RP SER-159; SER-160; THR-243 AND SER-249, AND MASS SPECTROMETRY. RX PubMed=17287358; DOI=10.1073/pnas.0607084104; RA Chi A., Huttenhower C., Geer L.Y., Coon J.J., Syka J.E.P., Bai D.L., RA Shabanowitz J., Burke D.J., Troyanskaya O.G., Hunt D.F.; RT "Analysis of phosphorylation sites on proteins from Saccharomyces RT cerevisiae by electron transfer dissociation (ETD) mass RT spectrometry."; RL Proc. Natl. Acad. Sci. U.S.A. 104:2193-2198(2007). RN [12] RP 3D-STRUCTURE MODELING OF 2-245, AND ELECTRON MICROSCOPY. RX MEDLINE=21558141; PubMed=11701127; DOI=10.1016/S0092-8674(01)00539-6; RA Spahn C.M.T., Beckmann R., Eswar N., Penczek P.A., Sali A., Blobel G., RA Frank J.; RT "Structure of the 80S ribosome from Saccharomyces cerevisiae -- tRNA- RT ribosome and subunit-subunit interactions."; RL Cell 107:373-386(2001). RN [13] RP 3D-STRUCTURE MODELING, AND ELECTRON MICROSCOPY. RX PubMed=14976550; DOI=10.1038/sj.emboj.7600102; RA Spahn C.M.T., Gomez-Lorenzo M.G., Grassucci R.A., Joergensen R., RA Andersen G.R., Beckmann R., Penczek P.A., Ballesta J.P.G., Frank J.; RT "Domain movements of elongation factor eEF2 and the eukaryotic 80S RT ribosome facilitate tRNA translocation."; RL EMBO J. 23:1008-1019(2004). CC -!- SUBUNIT: Component of the large ribosomal subunit. Mature CC ribosomes consist of a small (40S) and a large (60S) subunit. The CC 40S subunit contains 32 different proteins (encoded by 56 genes) CC and 1 molecule of RNA (18S). The 60S subunit contains 46 different CC proteins (encoded by 81 genes) and 3 molecules of RNA (25S, 5.8S CC and 5S). CC -!- SUBCELLULAR LOCATION: Cytoplasm. CC -!- MISCELLANEOUS: Present with 4050 molecules/cell in log phase SD CC medium. CC -!- MISCELLANEOUS: There are 2 genes for L2 in yeast. CC -!- SIMILARITY: Belongs to the ribosomal protein L2P family. CC --------------------------------------------------------------------------- CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms CC Distributed under the Creative Commons Attribution (CC BY 4.0) License CC --------------------------------------------------------------------------- DR EMBL; U17359; AAA92283.1; -; Genomic_DNA. DR EMBL; U17360; AAA92284.1; -; mRNA. DR EMBL; D50617; -; NOT_ANNOTATED_CDS; Genomic_DNA. DR EMBL; Z46881; CAA86974.1; -; Genomic_DNA. DR EMBL; U05820; AAA17418.1; -; Genomic_DNA. DR PIR; S50243; S50243. DR RefSeq; NP_012246.1; -. DR RefSeq; NP_116688.1; -. DR PDB; 1K5Y; Model; -; B=2-245. DR PDB; 1S1I; EM; 11.70 A; B=2-254. DR PDBsum; 1K5Y; -. DR PDBsum; 1S1I; -. DR DIP; DIP:6777N; -. DR IntAct; P05736; -. DR PeptideAtlas; P05736; -. DR Ensembl; YFR031C-A; Saccharomyces cerevisiae. DR Ensembl; YIL018W; Saccharomyces cerevisiae. DR GeneID; 850590; -. DR GeneID; 854794; -. DR KEGG; sce:YFR031C-A; -. DR KEGG; sce:YIL018W; -. DR CYGD; YFR031c-a; -. DR CYGD; YIL018w; -. DR SGD; S000002104; RPL2A. DR SGD; S000001280; RPL2B. DR HOGENOM; P05736; -. DR LinkHub; P05736; -. DR GermOnline; YFR031C-A; Saccharomyces cerevisiae. DR GermOnline; YIL018W; Saccharomyces cerevisiae. DR GO; GO:0042493; P:response to drug; IMP:SGD. DR InterPro; IPR012340; NA-bd_OB-fold. DR InterPro; IPR002171; Ribosomal_L2. DR InterPro; IPR014726; Ribosomal_L2_3. DR Gene3D; G3DSA:2.40.50.140; OB_NA_bd_sub; 1. DR Gene3D; G3DSA:4.10.950.10; Ribosomal_L2; 1. DR PANTHER; PTHR13691; Ribosomal_L2; 1. DR Pfam; PF00181; Ribosomal_L2; 1. DR Pfam; PF03947; Ribosomal_L2_C; 1. DR PIRSF; PIRSF002158; Ribosomal_L2; 1. DR PROSITE; PS00467; RIBOSOMAL_L2; 1. PE 1: Evidence at protein level; KW 3D-structure; Complete proteome; Cytoplasm; Direct protein sequencing; KW Phosphoprotein; Ribonucleoprotein; Ribosomal protein; RNA-binding; KW rRNA-binding. FT INIT_MET 1 1 Removed. FT CHAIN 2 254 60S ribosomal protein L2. FT /FTId=PRO_0000129761. FT MOD_RES 20 20 Phosphothreonine. FT MOD_RES 52 52 Phosphoserine. FT MOD_RES 84 84 Phosphothreonine. FT MOD_RES 159 159 Phosphoserine. FT MOD_RES 160 160 Phosphoserine. FT MOD_RES 243 243 Phosphothreonine. FT MOD_RES 249 249 Phosphoserine. SQ SEQUENCE 254 AA; 27408 MW; 5159A980574DF05A CRC64; MGRVIRNQRK GAGSIFTSHT RLRQGAAKLR TLDYAERHGY IRGIVKQIVH DSGRGAPLAK VVFRDPYKYR LREEIFIANE GVHTGQFIYA GKKASLNVGN VLPLGSVPEG TIVSNVEEKP GDRGALARAS GNYVIIIGHN PDENKTRVRL PSGAKKVISS DARGVIGVIA GGGRVDKPLL KAGRAFHKYR LKRNSWPKTR GVAMNPVDHP HGGGNHQHIG KASTISRGAV SGQKAGLIAA RRTGLLRGSQ KTQD //