ID NGF_CHICK Reviewed; 243 AA. AC P05200; DT 13-AUG-1987, integrated into UniProtKB/Swiss-Prot. DT 13-AUG-1987, sequence version 1. DT 22-APR-2020, entry version 134. DE RecName: Full=Beta-nerve growth factor; DE Short=Beta-NGF; DE Flags: Precursor; GN Name=NGF; Synonyms=NGFB; OS Gallus gallus (Chicken). OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; OC Archelosauria; Archosauria; Dinosauria; Saurischia; Theropoda; OC Coelurosauria; Aves; Neognathae; Galloanserae; Galliformes; Phasianidae; OC Phasianinae; Gallus. OX NCBI_TaxID=9031; RN [1] RP NUCLEOTIDE SEQUENCE [GENOMIC DNA]. RX PubMed=3017695; DOI=10.1002/j.1460-2075.1986.tb04386.x; RA Ebendahl T., Larhammar D., Persson H.; RT "Structure and expression of the chicken beta nerve growth factor gene."; RL EMBO J. 5:1483-1487(1986). RN [2] RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 118-243. RX PubMed=3720959; DOI=10.1016/0014-5793(86)81441-7; RA Wion D., Perret C., Frechin N., Keller A., Behar G., Brachet P., RA Auffray C.; RT "Molecular cloning of the avian beta-nerve growth factor gene: RT transcription in brain."; RL FEBS Lett. 203:82-86(1986). RN [3] RP NUCLEOTIDE SEQUENCE [MRNA] OF 121-243. RX PubMed=2427334; DOI=10.1002/j.1460-2075.1986.tb04387.x; RA Meier R., Becker-Andre M., Gotz R., Heumann R., Shaw A., Thoenen H.; RT "Molecular cloning of bovine and chick nerve growth factor (NGF): RT delineation of conserved and unconserved domains and their relationship to RT the biological activity and antigenicity of NGF."; RL EMBO J. 5:1489-1493(1986). RN [4] RP NUCLEOTIDE SEQUENCE OF 181-222. RX PubMed=2025430; DOI=10.1016/0896-6273(91)90180-8; RA Hallboeoek F., Ibanez C.F., Persson H.; RT "Evolutionary studies of the nerve growth factor family reveal a novel RT member abundantly expressed in Xenopus ovary."; RL Neuron 6:845-858(1991). RN [5] RP MUTAGENESIS OF TRP-145; TYR-176; TRP-200; TRP-223; ARG-224 AND ARG-227. RX PubMed=2328722; DOI=10.1002/j.1460-2075.1990.tb08265.x; RA Ibanez C.F., Hallboeoek F., Ebendal T., Persson H.; RT "Structure-function studies of nerve growth factor: functional importance RT of highly conserved amino acid residues."; RL EMBO J. 9:1477-1483(1990). CC -!- FUNCTION: Nerve growth factor is important for the development and CC maintenance of the sympathetic and sensory nervous systems. CC Extracellular ligand for the NTRK1 and NGFR receptors, activates CC cellular signaling cascades through those receptor tyrosine kinase to CC regulate neuronal proliferation, differentiation and survival. CC -!- SUBUNIT: Homodimer. CC -!- SUBCELLULAR LOCATION: Secreted. CC -!- SIMILARITY: Belongs to the NGF-beta family. {ECO:0000305}. CC -!- SEQUENCE CAUTION: CC Sequence=CAA27633.1; Type=Erroneous initiation; Evidence={ECO:0000305}; CC --------------------------------------------------------------------------- CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms CC Distributed under the Creative Commons Attribution (CC BY 4.0) License CC --------------------------------------------------------------------------- DR EMBL; X04003; CAA27633.1; ALT_INIT; Genomic_DNA. DR EMBL; X04067; CAA27703.1; -; Genomic_DNA. DR EMBL; M26810; AAA48984.1; -; mRNA. DR PIR; A26311; A26311. DR STRING; 9031.ENSGALP00000004030; -. DR PaxDb; P05200; -. DR PRIDE; P05200; -. DR eggNOG; ENOG410IIPQ; Eukaryota. DR eggNOG; ENOG4111F87; LUCA. DR HOGENOM; CLU_059942_1_1_1; -. DR InParanoid; P05200; -. DR PhylomeDB; P05200; -. DR Proteomes; UP000000539; Unplaced. DR Bgee; ENSGALG00000002556; Expressed in heart and 6 other tissues. DR GO; GO:0030424; C:axon; IBA:GO_Central. DR GO; GO:0031410; C:cytoplasmic vesicle; IBA:GO_Central. DR GO; GO:0030425; C:dendrite; IBA:GO_Central. DR GO; GO:0005615; C:extracellular space; IBA:GO_Central. DR GO; GO:0008021; C:synaptic vesicle; IBA:GO_Central. DR GO; GO:0008083; F:growth factor activity; IBA:GO_Central. DR GO; GO:0005163; F:nerve growth factor receptor binding; IBA:GO_Central. DR GO; GO:0007613; P:memory; IBA:GO_Central. DR GO; GO:0050804; P:modulation of chemical synaptic transmission; IBA:GO_Central. DR GO; GO:0043524; P:negative regulation of neuron apoptotic process; IBA:GO_Central. DR GO; GO:0021675; P:nerve development; IBA:GO_Central. DR GO; GO:0038180; P:nerve growth factor signaling pathway; IBA:GO_Central. DR GO; GO:0048812; P:neuron projection morphogenesis; IBA:GO_Central. DR GO; GO:0007422; P:peripheral nervous system development; IBA:GO_Central. DR GO; GO:0048672; P:positive regulation of collateral sprouting; IBA:GO_Central. DR GO; GO:0045664; P:regulation of neuron differentiation; IBA:GO_Central. DR GO; GO:0007169; P:transmembrane receptor protein tyrosine kinase signaling pathway; IBA:GO_Central. DR Gene3D; 2.10.90.10; -; 1. DR InterPro; IPR029034; Cystine-knot_cytokine. DR InterPro; IPR020408; Nerve_growth_factor-like. DR InterPro; IPR002072; Nerve_growth_factor-rel. DR InterPro; IPR020425; Nerve_growth_factor_bsu. DR InterPro; IPR019846; Nerve_growth_factor_CS. DR PANTHER; PTHR11589; PTHR11589; 1. DR Pfam; PF00243; NGF; 1. DR PIRSF; PIRSF001789; NGF; 1. DR PRINTS; PR00268; NGF. DR PRINTS; PR01913; NGFBETA. DR SMART; SM00140; NGF; 1. DR SUPFAM; SSF57501; SSF57501; 1. DR PROSITE; PS00248; NGF_1; 1. DR PROSITE; PS50270; NGF_2; 1. PE 1: Evidence at protein level; KW Cleavage on pair of basic residues; Disulfide bond; Growth factor; KW Reference proteome; Secreted; Signal. FT SIGNAL 1..22 FT /evidence="ECO:0000255" FT PROPEP 23..125 FT /id="PRO_0000019611" FT CHAIN 126..243 FT /note="Beta-nerve growth factor" FT /id="PRO_0000019612" FT DISULFID 139..204 FT /evidence="ECO:0000250" FT DISULFID 182..232 FT /evidence="ECO:0000250" FT DISULFID 192..234 FT /evidence="ECO:0000250" FT MUTAGEN 145 FT /note="W->F: Diminishes receptor binding; when associated FT with F-200 and F-223." FT /evidence="ECO:0000269|PubMed:2328722" FT MUTAGEN 176 FT /note="Y->F: No effect." FT /evidence="ECO:0000269|PubMed:2328722" FT MUTAGEN 200 FT /note="W->F: Diminishes receptor binding; when associated FT with F-145 and F-223." FT /evidence="ECO:0000269|PubMed:2328722" FT MUTAGEN 223 FT /note="W->F: Diminishes receptor binding; when associated FT with F-145 and F-200." FT /evidence="ECO:0000269|PubMed:2328722" FT MUTAGEN 224 FT /note="R->G: Impairs receptor binding; when associated with FT G-227." FT /evidence="ECO:0000269|PubMed:2328722" FT MUTAGEN 224 FT /note="R->K: No effect." FT /evidence="ECO:0000269|PubMed:2328722" FT MUTAGEN 227 FT /note="R->G: Impairs receptor binding; when associated with FT G-224." FT /evidence="ECO:0000269|PubMed:2328722" SQ SEQUENCE 243 AA; 27138 MW; 74C306CB2079DA07 CRC64; MHSVMSMLYY TLIIAFLIGT QAAPKSEDNG PLEYPAEHSL PSTQQSNGQH IAKAAPQTTH GRFAWMPDGT EDLNIAMDQN FFKKKRFRSS RVLFSTQPPP VSRKGQSTGF LSSAVSLNRT ARTKRTAHPV LHRGEFSVCD SVSMWVGDKT TATDIKGKEV TVLGEVNINN NVFKQYFFET KCRDPRPVSS GCRGIDAKHW NSYCTTTHTF VKALTMEGKQ AAWRFIRIDT ACVCVLSRKS GRP //