ID CDC10_SCHPO Reviewed; 767 AA. AC P01129; Q9UU04; DT 21-JUL-1986, integrated into UniProtKB/Swiss-Prot. DT 21-JUL-1986, sequence version 1. DT 16-DEC-2008, entry version 82. DE RecName: Full=Start control protein cdc10; GN Name=cdc10; ORFNames=SPBC336.12c; OS Schizosaccharomyces pombe (Fission yeast). OC Eukaryota; Fungi; Dikarya; Ascomycota; Taphrinomycotina; OC Schizosaccharomycetes; Schizosaccharomycetales; OC Schizosaccharomycetaceae; Schizosaccharomyces. OX NCBI_TaxID=4896; RN [1] RP NUCLEOTIDE SEQUENCE [GENOMIC DNA]. RX MEDLINE=85257476; PubMed=4018034; RA Aves S.J., Durkacz B.W., Carr A., Nurse P.; RT "Cloning, sequencing and transcriptional control of the RT Schizosaccharomyces pombe cdc10 'start' gene."; RL EMBO J. 4:457-463(1985). RN [2] RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. RC STRAIN=ATCC 38366 / 972; RX MEDLINE=21848401; PubMed=11859360; DOI=10.1038/nature724; RA Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A., RA Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S., RA Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., RA Collins M., Connor R., Cronin A., Davis P., Feltwell T., Fraser A., RA Gentles S., Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., RA Holroyd S., Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., RA James K.D., Jones L., Jones M., Leather S., McDonald S., McLean J., RA Mooney P., Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., RA Oliver K., O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., RA Rutherford K.M., Rutter S., Saunders D., Seeger K., Sharp S., RA Skelton J., Simmonds M.N., Squares R., Squares S., Stevens K., RA Taylor K., Taylor R.G., Tivey A., Walsh S.V., Warren T., Whitehead S., RA Woodward J.R., Volckaert G., Aert R., Robben J., Grymonprez B., RA Weltjens I., Vanstreels E., Rieger M., Schaefer M., Mueller-Auer S., RA Gabel C., Fuchs M., Duesterhoeft A., Fritzc C., Holzer E., Moestl D., RA Hilbert H., Borzym K., Langer I., Beck A., Lehrach H., Reinhardt R., RA Pohl T.M., Eger P., Zimmermann W., Wedler H., Wambutt R., Purnelle B., RA Goffeau A., Cadieu E., Dreano S., Gloux S., Lelaure V., Mottier S., RA Galibert F., Aves S.J., Xiang Z., Hunt C., Moore K., Hurst S.M., RA Lucas M., Rochet M., Gaillardin C., Tallada V.A., Garzon A., Thode G., RA Daga R.R., Cruzado L., Jimenez J., Sanchez M., del Rey F., Benito J., RA Dominguez A., Revuelta J.L., Moreno S., Armstrong J., Forsburg S.L., RA Cerutti L., Lowe T., McCombie W.R., Paulsen I., Potashkin J., RA Shpakovski G.V., Ussery D., Barrell B.G., Nurse P.; RT "The genome sequence of Schizosaccharomyces pombe."; RL Nature 415:871-880(2002). RN [3] RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA] OF 76-310, AND RP SUBCELLULAR LOCATION. RC STRAIN=ATCC 38364 / 968; RX MEDLINE=20223868; PubMed=10759889; RX DOI=10.1046/j.1365-2443.2000.00317.x; RA Ding D.-Q., Tomita Y., Yamamoto A., Chikashige Y., Haraguchi T., RA Hiraoka Y.; RT "Large-scale screening of intracellular protein localization in living RT fission yeast cells by the use of a GFP-fusion genomic DNA library."; RL Genes Cells 5:169-190(2000). RN [4] RP FUNCTION. RX PubMed=8532516; DOI=10.1093/nar/23.23.4761; RA McInerny C.J., Kersey P.J., Creanor J., Fantes P.A.; RT "Positive and negative roles for cdc10 in cell cycle gene RT expression."; RL Nucleic Acids Res. 23:4761-4768(1995). RN [5] RP FUNCTION, AND INTERACTION WITH POL5. RX PubMed=16816948; DOI=10.1007/s00438-006-0144-6; RA Nadeem F.K., Blair D., McInerny C.J.; RT "Pol5p, a novel binding partner to Cdc10p in fission yeast involved in RT rRNA production."; RL Mol. Genet. Genomics 276:391-401(2006). RN [6] RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-252, AND MASS RP SPECTROMETRY. RX PubMed=18257517; DOI=10.1021/pr7006335; RA Wilson-Grady J.T., Villen J., Gygi S.P.; RT "Phosphoproteome analysis of fission yeast."; RL J. Proteome Res. 7:1088-1097(2008). CC -!- FUNCTION: Major component of the cell cycle transcription factor CC complex MBF (MCB binding factor, also known as DSC1), that CC controls G1-S phase specific gene expression. Involved in the CC control of rRNA production, via interaction with pol5. May be CC involved in the transcriptional regulation of the cdc22 and cdt1 CC genes. In fission yeast, two genes, cdc10 and cdc2, are required CC for the cell cycle control called start, the point early in the G1 CC phase at which cells become committed to the mitotic cycle. CC -!- SUBUNIT: DSC1 contains cdc10 and sct1/res1. Interacts with pol5. CC -!- INTERACTION: CC O60094:pol5; NbExp=1; IntAct=EBI-1009350, EBI-1009362; CC P41412:res2; NbExp=1; IntAct=EBI-1009350, EBI-1149177; CC -!- SUBCELLULAR LOCATION: Nucleus. CC -!- SIMILARITY: Contains 2 ANK repeats. CC -!- SIMILARITY: Contains 1 HTH APSES-type DNA-binding domain. CC --------------------------------------------------------------------------- CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms CC Distributed under the Creative Commons Attribution (CC BY 4.0) License CC --------------------------------------------------------------------------- DR EMBL; X02175; CAA26116.1; -; Genomic_DNA. DR EMBL; CU329671; CAB58164.1; -; Genomic_DNA. DR EMBL; AB027889; BAA87193.1; -; Genomic_DNA. DR PIR; A01382; COZPCD. DR RefSeq; NP_596132.1; -. DR HSSP; P09959; 1SW6. DR DIP; DIP:353N; -. DR IntAct; P01129; 2. DR GeneID; 2540234; -. DR KEGG; spo:SPBC336.12c; -. DR NMPDR; fig|4896.1.peg.1998; -. DR GeneDB_Spombe; SPBC336.12c; -. DR BioCyc; SPOM-XXX-01:SPOM-XXX-01-004171-MON; -. DR ArrayExpress; P01129; -. DR GO; GO:0005737; C:cytoplasm; IDA:GeneDB_SPombe. DR GO; GO:0030907; C:MBF transcription complex; TAS:GeneDB_SPombe. DR GO; GO:0005515; F:protein binding; IPI:UniProtKB. DR GO; GO:0003700; F:transcription factor activity; IEA:InterPro. DR GO; GO:0051301; P:cell division; IEA:UniProtKB-KW. DR GO; GO:0051038; P:negative regulation of transcription, meiotic; IDA:UniProtKB. DR GO; GO:0007068; P:negative regulation of transcription, mitotic; IDA:UniProtKB. DR GO; GO:0051039; P:positive regulation of transcription, meiotic; IDA:UniProtKB. DR GO; GO:0045897; P:positive regulation of transcription, mitotic; IDA:UniProtKB. DR GO; GO:0000083; P:regulation of transcription of G1/S-phase o...; TAS:GeneDB_SPombe. DR GO; GO:0009303; P:rRNA transcription; IDA:UniProtKB. DR GO; GO:0000084; P:S phase of mitotic cell cycle; IGI:GeneDB_SPombe. DR InterPro; IPR002110; ANK. DR InterPro; IPR016144; Mlu1-box-bd_prot_DNA-bd. DR InterPro; IPR003163; Yeast_DNA_bd. DR Gene3D; G3DSA:1.25.40.20; ANK; 1. DR Gene3D; G3DSA:3.10.260.10; Mlu1-box-bd_prot_DNA-bd; 1. DR Pfam; PF00023; Ank; 3. DR Pfam; PF02292; APSES; 1. DR PRINTS; PR01415; ANKYRIN. DR SMART; SM00248; ANK; 2. DR PROSITE; PS50297; ANK_REP_REGION; 1. DR PROSITE; PS50088; ANK_REPEAT; 2. DR PROSITE; PS51299; HTH_APSES; 1. PE 1: Evidence at protein level; KW ANK repeat; Cell cycle; Cell division; Complete proteome; Mitosis; KW Nucleus; Phosphoprotein; Repeat. FT CHAIN 1 767 Start control protein cdc10. FT /FTId=PRO_0000067060. FT DOMAIN 66 173 HTH APSES-type. FT REPEAT 356 385 ANK 1. FT REPEAT 483 512 ANK 2. FT DNA_BIND 98 119 H-T-H motif (By similarity). FT MOTIF 261 264 Nuclear localization signal (Potential). FT MOD_RES 252 252 Phosphoserine. SQ SEQUENCE 767 AA; 85513 MW; 0E514EE1BE218EFA CRC64; MASANFIRQF ELGNDSFSYQ KRPEDEPSQP LSNRNINKLN DSSTLKDSSS RIFINSQVLR DGRPVELYAV ECSGMKYMEL SCGDNVALRR CPDSYFNISQ ILRLAGTSSS ENAKELDDII ESGDYENVDS KHPQIDGVWV PYDRAISIAK RYGVYEILQP LISFNLDLFP KFSKQQQIES SSISKNLNTS SFNTRSPLRN HNFSNPSKSS KNGVHTINNM QSSPSPSSSF LLPLTQIDSQ NVKRSNNYLS TSPPILEQRL KRHRIDVSDE DLHPSSQLND NEASSLFPDT PRLNHSLSFV SLVSSLPPLD QNIMQDYHTS KDILTSIFLD VNFADSSALE AKLSDSLDLD VPIDELGHAA LHWAAAVAKM PLLQALIHKG ANPLRGNLTG ETALMRSVLV TNHLNQNSFG DLLDLLYASL PCTDRAGRTV VHHICLTAGI KGRGSASRYY LETLLNWAKK HASGNNGYML KDFINYLNHQ DKNGDTALNI AARIGNKNIV EVLMQAGASA YIPNRAGLSV ANFGIFVENA LKQPEDSKQT KVSLMSENLS SKEKTAVPPR QKSRDIIASV TDVISSLDKD FQDEMAAKQS MIDSAYTQLR ESTKKLSDLR EQLHVSETQR TLFLELRQRC KNLMTSIEEQ KSELSNLYES FDPNGIHDSL SLDADAPFTV NENNNKNLSI AELKFQVAAY ERNEARLNEL ANKLWQRNSN IKSKCRRVVS LCTGVDESRV DSLLESLLQA VESDGQQGEV DMGRVAGFLR VVKEHQA //