ID S25BP_HUMAN Reviewed; 136 AA. AC O95295; DT 16-JAN-2004, integrated into UniProtKB/Swiss-Prot. DT 01-MAY-1999, sequence version 1. DT 21-AUG-2007, entry version 49. DE SNARE-associated protein Snapin (Synaptosomal-associated protein 25- DE binding protein) (SNAP-associated protein). GN Name=SNAPAP; Synonyms=SNAP25BP; OS Homo sapiens (Human). OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; OC Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; OC Catarrhini; Hominidae; Homo. OX NCBI_TaxID=9606; RN [1] RP NUCLEOTIDE SEQUENCE [MRNA]. RC TISSUE=Brain; RX MEDLINE=99211098; PubMed=10195194; DOI=10.1038/5673; RA Ilardi J.M., Mochida S., Sheng Z.-H.; RT "Snapin: a SNARE-associated protein implicated in synaptic RT transmission."; RL Nat. Neurosci. 2:119-124(1999). RN [2] RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. RC TISSUE=Adipose tissue; RX PubMed=14702039; DOI=10.1038/ng1285; RA Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., RA Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., RA Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S., RA Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., RA Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., RA Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., RA Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., RA Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., RA Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., RA Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., RA Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., RA Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., RA Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., RA Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., RA Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., RA Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., RA Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., RA Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., RA Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., RA Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., RA Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., RA Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., RA Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., RA Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., RA Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., RA Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., RA Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., RA Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.; RT "Complete sequencing and characterization of 21,243 full-length human RT cDNAs."; RL Nat. Genet. 36:40-45(2004). RN [3] RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. RA Kalnine N., Chen X., Rolfs A., Halleck A., Hines L., Eisenstein S., RA Koundinya M., Raphael J., Moreira D., Kelley T., LaBaer J., Lin Y., RA Phelan M., Farmer A.; RT "Cloning of human full-length CDSs in BD Creator(TM) system donor RT vector."; RL Submitted (MAY-2003) to the EMBL/GenBank/DDBJ databases. RN [4] RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. RC TISSUE=Lung carcinoma, and Melanoma; RX PubMed=15489334; DOI=10.1101/gr.2596504; RG The MGC Project Team; RT "The status, quality, and expansion of the NIH full-length cDNA RT project: the Mammalian Gene Collection (MGC)."; RL Genome Res. 14:2121-2127(2004). RN [5] RP INTERACTION WITH RGS7. RX PubMed=12659861; DOI=10.1016/S0006-291X(03)00400-5; RA Hunt R.A., Edris W., Chanda P.K., Nieuwenhuijsen B., Young K.H.; RT "Snapin interacts with the N-terminus of regulator of G protein RT signaling 7."; RL Biochem. Biophys. Res. Commun. 303:594-599(2003). RN [6] RP IDENTIFICATION IN BLOC1 COMPLEX. RX PubMed=15102850; DOI=10.1074/jbc.M402513200; RA Starcevic M., Dell'Angelica E.C.; RT "Identification of snapin and three novel proteins (BLOS1, BLOS2, and RT BLOS3/reduced pigmentation) as subunits of biogenesis of lysosome- RT related organelles complex-1 (BLOC-1)."; RL J. Biol. Chem. 279:28393-28401(2004). RN [7] RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-133, AND MASS RP SPECTROMETRY. RX PubMed=17287340; DOI=10.1073/pnas.0611217104; RA Molina H., Horn D.M., Tang N., Mathivanan S., Pandey A.; RT "Global proteomic profiling of phosphopeptides using electron transfer RT dissociation tandem mass spectrometry."; RL Proc. Natl. Acad. Sci. U.S.A. 104:2199-2204(2007). CC -!- FUNCTION: May modulate a step between vesicle priming, fusion and CC calcium-dependent neurotransmitter release by potentiating the CC interaction of synaptotagmins with the SNAREs and the plasma- CC membrane-associated protein SNAP25. Its phosphorylation state CC influences exocytotic protein interactions and may regulate CC synaptic vesicle exocytosis. May also have a role in the CC mechanisms of SNARE-mediated membrane fusion in non-neuronal cells CC (By similarity). CC -!- SUBUNIT: Associates with the SNARE complex. Interacts with SNAP23, CC SNAP25 and STX4A but not with STX1A, VAMP2 and SYT1. CC Phosphorylation increases its interaction with SNAP25 (By CC similarity). Interacts with RGS7. Component of the biogenesis of CC lysosome-related organelles (BLOC-1) complex which is required for CC normal biogenesis of specialized organelles of the endosomal- CC lysosomal system. CC -!- INTERACTION: CC Q6QNY1:BLOC1S2; NbExp=1; IntAct=EBI-296723, EBI-465872; CC Q6QNY0:BLOC1S3; NbExp=1; IntAct=EBI-296723, EBI-465930; CC Q96EV8:DTNBP1; NbExp=1; IntAct=EBI-296723, EBI-465804; CC Q9UL45:PLDN; NbExp=1; IntAct=EBI-296723, EBI-465781; CC -!- SUBCELLULAR LOCATION: Cytoplasm (By similarity). Membrane; CC Peripheral membrane protein (By similarity). Cytoplasmic vesicle CC membrane (By similarity). Note=May be cytoplasmic and peripheral CC membrane bound or anchored to the vesicular membrane through an N- CC terminal signal anchor (By similarity). CC -!- PTM: Phosphorylated (By similarity). CC --------------------------------------------------------------------------- CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms CC Distributed under the Creative Commons Attribution (CC BY 4.0) License CC --------------------------------------------------------------------------- DR EMBL; AF086837; AAD11417.1; -; mRNA. DR EMBL; AK024555; BAB14927.1; -; mRNA. DR EMBL; BT006753; AAP35399.1; -; mRNA. DR EMBL; BC000761; AAH00761.1; -; mRNA. DR EMBL; BC004494; AAH04494.1; -; mRNA. DR UniGene; Hs.32018; -. DR IntAct; O95295; -. DR PeptideAtlas; O95295; -. DR Ensembl; ENSG00000143553; Homo sapiens. DR KEGG; hsa:23557; -. DR H-InvDB; HIX0001087; -. DR HGNC; HGNC:17145; SNAPAP. DR MIM; 607007; gene. DR PharmGKB; PA134896719; -. DR ArrayExpress; O95295; -. DR GermOnline; ENSG00000143553; Homo sapiens. DR GO; GO:0008021; C:synaptic vesicle; TAS:ProtInc. DR GO; GO:0000149; F:SNARE binding; TAS:ProtInc. DR GO; GO:0006886; P:intracellular protein transport; TAS:ProtInc. DR GO; GO:0007269; P:neurotransmitter secretion; TAS:ProtInc. PE 1: Evidence at protein level; KW Cell junction; Coiled coil; Cytoplasm; Cytoplasmic vesicle; KW Exocytosis; Membrane; Phosphorylation; Polymorphism; Synapse; KW Synaptosome. FT CHAIN 1 136 SNARE-associated protein Snapin. FT /FTId=PRO_0000097556. FT COILED 37 126 Potential. FT MOD_RES 50 50 Phosphoserine; by PKA (By similarity). FT MOD_RES 133 133 Phosphoserine. FT VARIANT 112 112 S -> C (in dbSNP:rs1802461). FT /FTId=VAR_017423. SQ SEQUENCE 136 AA; 14874 MW; 3EA402AC53C81FFF CRC64; MAGAGSAAVS GAGTPVAGPT GRDLFAEGLL EFLRPAVQQL DSHVHAVRES QVELREQIDN LATELCRINE DQKVALDLDP YVKKLLNARR RVVLVNNILQ NAQERLRRLN HSVAKETARR RAMLDSGIYP PGSPGK //