ID SPSB2_MOUSE STANDARD; PRT; 264 AA. AC O88838; Q8CBA5; Q91Z15; DT 30-MAY-2006, integrated into UniProtKB/Swiss-Prot. DT 01-NOV-1998, sequence version 1. DT 05-SEP-2006, entry version 38. DE SPRY domain-containing SOCS box protein 2 (SSB-2) (Gene-rich cluster DE protein C9). GN Name=Spsb2; Synonyms=Grcc9, Ssb2; OS Mus musculus (Mouse). OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; OC Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Sciurognathi; OC Muroidea; Muridae; Murinae; Mus. OX NCBI_TaxID=10090; RN [1] RP NUCLEOTIDE SEQUENCE [GENOMIC DNA]. RX MEDLINE=98112780; PubMed=9445485; RA Ansari-Lari M.A., Oeltjen J.C., Schwartz S., Zhang Z., Muzny D.M., RA Lu J., Gorrell J.H., Chinault A.C., Belmont J.W., Miller W., RA Gibbs R.A.; RT "Comparative sequence analysis of a gene-rich cluster at human RT chromosome 12p13 and its syntenic region in mouse chromosome 6."; RL Genome Res. 8:29-40(1998). RN [2] RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1). RA Friedel E.J., Nicholson S.E., Nicola N.A., Kile B.T., Hilton D.J.; RT "SOCS box proteins."; RL Submitted (JUL-2001) to the EMBL/GenBank/DDBJ databases. RN [3] RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). RC STRAIN=C57BL/6J; TISSUE=Bone; RX PubMed=16141072; DOI=10.1126/science.1112014; RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., RA Davis M.J., Wilming L.G., Aidinis V., Allen J.E., RA Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., RA Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., RA Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., RA Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., RA di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., RA Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., RA Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., RA Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., RA Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., RA Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., RA Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., RA Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., RA Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., RA Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., RA Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., RA Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., RA Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., RA Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., RA Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., RA Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., RA Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., RA Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., RA Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., RA Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., RA Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., RA Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., RA Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., RA Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., RA Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., RA Hayashizaki Y.; RT "The transcriptional landscape of the mammalian genome."; RL Science 309:1559-1563(2005). RN [4] RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2). RC STRAIN=FVB/N; TISSUE=Mammary gland, and Mammary tumor; RX MEDLINE=22388257; PubMed=12477932; DOI=10.1073/pnas.242603899; RA Strausberg R.L., Feingold E.A., Grouse L.H., Derge J.G., RA Klausner R.D., Collins F.S., Wagner L., Shenmen C.M., Schuler G.D., RA Altschul S.F., Zeeberg B., Buetow K.H., Schaefer C.F., Bhat N.K., RA Hopkins R.F., Jordan H., Moore T., Max S.I., Wang J., Hsieh F., RA Diatchenko L., Marusina K., Farmer A.A., Rubin G.M., Hong L., RA Stapleton M., Soares M.B., Bonaldo M.F., Casavant T.L., Scheetz T.E., RA Brownstein M.J., Usdin T.B., Toshiyuki S., Carninci P., Prange C., RA Raha S.S., Loquellano N.A., Peters G.J., Abramson R.D., Mullahy S.J., RA Bosak S.A., McEwan P.J., McKernan K.J., Malek J.A., Gunaratne P.H., RA Richards S., Worley K.C., Hale S., Garcia A.M., Gay L.J., Hulyk S.W., RA Villalon D.K., Muzny D.M., Sodergren E.J., Lu X., Gibbs R.A., RA Fahey J., Helton E., Ketteman M., Madan A., Rodrigues S., Sanchez A., RA Whiting M., Madan A., Young A.C., Shevchenko Y., Bouffard G.G., RA Blakesley R.W., Touchman J.W., Green E.D., Dickson M.C., RA Rodriguez A.C., Grimwood J., Schmutz J., Myers R.M., RA Butterfield Y.S.N., Krzywinski M.I., Skalska U., Smailus D.E., RA Schnerch A., Schein J.E., Jones S.J.M., Marra M.A.; RT "Generation and initial analysis of more than 15,000 full-length human RT and mouse cDNA sequences."; RL Proc. Natl. Acad. Sci. U.S.A. 99:16899-16903(2002). RN [5] RP STRUCTURE BY NMR OF 12-224, INTERACTION WITH PAWR AND MET, AND RP MUTAGENESIS OF TYR-120; 123-LEU--GLY-125; 141-TYR--GLN-143; RP 151-GLN-TYR-152; 188-GLY-PRO-189 AND VAL-206. RX PubMed=16369487; DOI=10.1038/nsmb1034; RA Masters S.L., Yao S., Willson T.A., Zhang J.-G., Palmer K.R., RA Smith B.J., Babon J.J., Nicola N.A., Norton R.S., Nicholson S.E.; RT "The SPRY domain of SSB-2 adopts a novel fold that presents conserved RT Par-4-binding residues."; RL Nat. Struct. Mol. Biol. 13:77-84(2006). CC -!- SUBUNIT: Interacts with PAWR and MET. CC -!- SUBCELLULAR LOCATION: Cytoplasm (Probable). CC -!- ALTERNATIVE PRODUCTS: CC Event=Alternative splicing; Named isoforms=2; CC Name=1; CC IsoId=O88838-1; Sequence=Displayed; CC Name=2; CC IsoId=O88838-2; Sequence=VSP_018613; CC Note=No experimental confirmation available; CC -!- SIMILARITY: Contains 1 B30.2/SPRY domain. CC -!- SIMILARITY: Contains 1 SOCS box domain. CC --------------------------------------------------------------------------- CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms CC Distributed under the Creative Commons Attribution (CC BY 4.0) License CC --------------------------------------------------------------------------- DR EMBL; AC002397; AAC36017.1; -; Genomic_DNA. DR EMBL; AF403037; AAL57356.1; -; mRNA. DR EMBL; AK036451; BAC29435.1; -; mRNA. DR EMBL; AK148177; BAE28397.1; -; mRNA. DR EMBL; BC002005; AAH02005.1; -; mRNA. DR EMBL; BC010305; AAH10305.1; -; mRNA. DR UniGene; Mm.261906; -. DR PDB; 2AFJ; NMR; A=12-224. DR Ensembl; ENSMUSG00000038451; Mus musculus. DR MGI; MGI:1315199; Spsb2. DR ArrayExpress; O88838; -. DR RZPD-ProtExp; IOM18334; -. DR InterPro; IPR001870; B302. DR InterPro; IPR001496; SOCS_C. DR InterPro; IPR003877; SPRY_rcpt. DR InterPro; IPR001680; WD40. DR Pfam; PF07525; SOCS_box; 1. DR Pfam; PF00622; SPRY; 1. DR SMART; SM00253; SOCS; 1. DR SMART; SM00449; SPRY; 1. DR PROSITE; PS50188; B302_SPRY; 1. DR PROSITE; PS50225; SOCS; 1. KW 3D-structure; Alternative splicing. FT CHAIN 1 264 SPRY domain-containing SOCS box protein FT 2. FT /FTId=PRO_0000238475. FT DOMAIN 26 221 B30.2/SPRY. FT DOMAIN 222 264 SOCS box. FT VAR_SEQ 222 264 VEEPQSLLHLSRLCVRHALGDTRLGQISTLPLPPAMKRYLL FT YK -> GEIRTRCGEITTLGNGLGWKLMVGAQEVGFLSLWP FT VT (in isoform 2). FT /FTId=VSP_018613. FT MUTAGEN 120 120 Y->A: Loss of interaction with PARW. No FT effect on interaction with MET. FT MUTAGEN 123 125 LLG->AAA: Loss of interaction with MET FT and PARW. FT MUTAGEN 141 143 YHQ->AAA: Strongly reduced interaction FT with MET and PARW. FT MUTAGEN 151 152 QY->AA: Loss of interaction with MET and FT PARW. FT MUTAGEN 188 189 GP->AA: Strongly reduced interaction with FT MET. Loss of interaction with PARW. FT MUTAGEN 206 206 V->A: Loss of interaction with PARW. No FT effect on interaction with MET. SQ SEQUENCE 264 AA; 28938 MW; E71985597D9B38CB CRC64; MGQTALARGS SSTPTSQALY SDFSPPEGLE ELLSAPPPDL VAQRHHGWNP KDCSENIDVK EGGLCFERRP VAQSTDGVRG KRGYSRGLHA WEISWPLEQR GTHAVVGVAT ALAPLQADHY AALLGSNSES WGWDIGRGKL YHQSKGLEAP QYPAGPQGEQ LVVPERLLVV LDMEEGTLGY SIGGTYLGPA FRGLKGRTLY PSVSAVWGQC QVRIRYMGER RVEEPQSLLH LSRLCVRHAL GDTRLGQIST LPLPPAMKRY LLYK //