ID BSSB_THAAR Reviewed; 74 AA. AC O87944; DT 05-SEP-2012, integrated into UniProtKB/Swiss-Prot. DT 01-NOV-1998, sequence version 1. DT 03-AUG-2022, entry version 41. DE RecName: Full=Benzylsuccinate synthase beta subunit; DE EC=4.1.99.11; GN Name=bssB; OS Thauera aromatica. OC Bacteria; Proteobacteria; Betaproteobacteria; Rhodocyclales; Zoogloeaceae; OC Thauera. OX NCBI_TaxID=59405; RN [1] RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], PARTIAL PROTEIN SEQUENCE, FUNCTION, RP CATALYTIC ACTIVITY, ACTIVITY REGULATION, BIOPHYSICOCHEMICAL PROPERTIES, RP PATHWAY, SUBUNIT, AND INDUCTION. RC STRAIN=DSM 6984 / CIP 107765 / K172; RX PubMed=9632263; DOI=10.1046/j.1365-2958.1998.00826.x; RA Leuthner B., Leutwein C., Schulz H., Horth P., Haehnel W., Schiltz E., RA Schagger H., Heider J.; RT "Biochemical and genetic characterization of benzylsuccinate synthase from RT Thauera aromatica: a new glycyl radical enzyme catalysing the first step in RT anaerobic toluene metabolism."; RL Mol. Microbiol. 28:615-628(1998). RN [2] RP INDUCTION. RC STRAIN=DSM 6984 / CIP 107765 / K172; RX PubMed=9741082; DOI=10.1111/j.1574-6968.1998.tb13180.x; RA Leuthner B., Heider J.; RT "A two-component system involved in regulation of anaerobic toluene RT metabolism in Thauera aromatica."; RL FEMS Microbiol. Lett. 166:35-41(1998). RN [3] RP INDUCTION. RC STRAIN=DSM 6984 / CIP 107765 / K172; RX PubMed=11807562; DOI=10.1007/s00203-001-0375-1; RA Hermuth K., Leuthner B., Heider J.; RT "Operon structure and expression of the genes for benzylsuccinate synthase RT in Thauera aromatica strain K172."; RL Arch. Microbiol. 177:132-138(2002). CC -!- FUNCTION: Catalyzes the addition of fumarate to the methyl group of CC toluene, leading to the formation of benzylsuccinate. CC {ECO:0000269|PubMed:9632263}. CC -!- CATALYTIC ACTIVITY: CC Reaction=fumarate + toluene = 2-benzylsuccinate; Xref=Rhea:RHEA:10416, CC ChEBI:CHEBI:17578, ChEBI:CHEBI:29806, ChEBI:CHEBI:57621; CC EC=4.1.99.11; Evidence={ECO:0000269|PubMed:9632263}; CC -!- ACTIVITY REGULATION: Activated by the benzylsuccinate synthase CC activating enzyme BssD. Rapidly inactivated by oxygen. CC {ECO:0000269|PubMed:9632263}. CC -!- BIOPHYSICOCHEMICAL PROPERTIES: CC pH dependence: CC Optimum pH is 8.0. {ECO:0000269|PubMed:9632263}; CC -!- PATHWAY: Xenobiotic degradation; toluene degradation. CC {ECO:0000269|PubMed:9632263}. CC -!- SUBUNIT: Heterohexamer composed of 2 alpha subunits, 2 beta subunits CC and 2 gamma subunits. {ECO:0000269|PubMed:9632263}. CC -!- INDUCTION: Induced by toluene, probably via the TdiR/TdiS two-component CC regulatory system. {ECO:0000269|PubMed:11807562, CC ECO:0000269|PubMed:9632263, ECO:0000269|PubMed:9741082}. CC --------------------------------------------------------------------------- CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms CC Distributed under the Creative Commons Attribution (CC BY 4.0) License CC --------------------------------------------------------------------------- DR EMBL; AJ001848; CAA05053.1; -; Genomic_DNA. DR AlphaFoldDB; O87944; -. DR SMR; O87944; -. DR KEGG; ag:CAA05053; -. DR BioCyc; MetaCyc:MON-671; -. DR BRENDA; 4.1.99.11; 6271. DR UniPathway; UPA00273; -. DR GO; GO:0018805; F:benzylsuccinate synthase activity; IEA:UniProtKB-EC. DR GO; GO:0042203; P:toluene catabolic process; IEA:UniProtKB-UniPathway. DR InterPro; IPR040565; BssB_TutG. DR Pfam; PF18512; BssB_TutG; 1. PE 1: Evidence at protein level; KW Aromatic hydrocarbons catabolism; Direct protein sequencing; Lyase. FT INIT_MET 1 FT /note="Removed" FT CHAIN 2..74 FT /note="Benzylsuccinate synthase beta subunit" FT /id="PRO_0000418873" SQ SEQUENCE 74 AA; 8752 MW; 41DD703589AAE6A0 CRC64; MSATPHTQVH WEENTARPCR KCKWQTPDPT DPLRGQCTVN RHAMGGVWKR WIRDVEHMTC SRHEEGELSF RDHV //