ID BSSB_THAAR Reviewed; 74 AA. AC O87944; DT 05-SEP-2012, integrated into UniProtKB/Swiss-Prot. DT 01-NOV-1998, sequence version 1. DT 05-DEC-2018, entry version 33. DE RecName: Full=Benzylsuccinate synthase beta subunit; DE EC=4.1.99.11; GN Name=bssB; OS Thauera aromatica. OC Bacteria; Proteobacteria; Betaproteobacteria; Rhodocyclales; OC Zoogloeaceae; Thauera. OX NCBI_TaxID=59405; RN [1] RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], PARTIAL PROTEIN SEQUENCE, FUNCTION, RP CATALYTIC ACTIVITY, ACTIVITY REGULATION, BIOPHYSICOCHEMICAL RP PROPERTIES, PATHWAY, SUBUNIT, AND INDUCTION. RC STRAIN=DSM 6984 / K172; RX PubMed=9632263; DOI=10.1046/j.1365-2958.1998.00826.x; RA Leuthner B., Leutwein C., Schulz H., Horth P., Haehnel W., Schiltz E., RA Schagger H., Heider J.; RT "Biochemical and genetic characterization of benzylsuccinate synthase RT from Thauera aromatica: a new glycyl radical enzyme catalysing the RT first step in anaerobic toluene metabolism."; RL Mol. Microbiol. 28:615-628(1998). RN [2] RP INDUCTION. RC STRAIN=DSM 6984 / K172; RX PubMed=9741082; DOI=10.1111/j.1574-6968.1998.tb13180.x; RA Leuthner B., Heider J.; RT "A two-component system involved in regulation of anaerobic toluene RT metabolism in Thauera aromatica."; RL FEMS Microbiol. Lett. 166:35-41(1998). RN [3] RP INDUCTION. RC STRAIN=DSM 6984 / K172; RX PubMed=11807562; DOI=10.1007/s00203-001-0375-1; RA Hermuth K., Leuthner B., Heider J.; RT "Operon structure and expression of the genes for benzylsuccinate RT synthase in Thauera aromatica strain K172."; RL Arch. Microbiol. 177:132-138(2002). CC -!- FUNCTION: Catalyzes the addition of fumarate to the methyl group CC of toluene, leading to the formation of benzylsuccinate. CC {ECO:0000269|PubMed:9632263}. CC -!- CATALYTIC ACTIVITY: CC Reaction=fumarate + toluene = 2-benzylsuccinate; CC Xref=Rhea:RHEA:10416, ChEBI:CHEBI:17578, ChEBI:CHEBI:29806, CC ChEBI:CHEBI:57621; EC=4.1.99.11; CC Evidence={ECO:0000269|PubMed:9632263}; CC -!- ACTIVITY REGULATION: Activated by the benzylsuccinate synthase CC activating enzyme BssD. Rapidly inactivated by oxygen. CC {ECO:0000269|PubMed:9632263}. CC -!- BIOPHYSICOCHEMICAL PROPERTIES: CC pH dependence: CC Optimum pH is 8.0. {ECO:0000269|PubMed:9632263}; CC -!- PATHWAY: Xenobiotic degradation; toluene degradation. CC {ECO:0000269|PubMed:9632263}. CC -!- SUBUNIT: Heterohexamer composed of 2 alpha subunits, 2 beta CC subunits and 2 gamma subunits. {ECO:0000269|PubMed:9632263}. CC -!- INDUCTION: Induced by toluene, probably via the TdiR/TdiS two- CC component regulatory system. {ECO:0000269|PubMed:11807562, CC ECO:0000269|PubMed:9632263, ECO:0000269|PubMed:9741082}. CC --------------------------------------------------------------------------- CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms CC Distributed under the Creative Commons Attribution (CC BY 4.0) License CC --------------------------------------------------------------------------- DR EMBL; AJ001848; CAA05053.1; -; Genomic_DNA. DR SMR; O87944; -. DR KEGG; ag:CAA05053; -. DR KO; K07540; -. DR BioCyc; MetaCyc:MONOMER-671; -. DR UniPathway; UPA00273; -. DR GO; GO:0018805; F:benzylsuccinate synthase activity; IEA:UniProtKB-EC. DR GO; GO:0042203; P:toluene catabolic process; IEA:UniProtKB-UniPathway. PE 1: Evidence at protein level; KW Aromatic hydrocarbons catabolism; Direct protein sequencing; Lyase. FT INIT_MET 1 1 Removed. FT CHAIN 2 74 Benzylsuccinate synthase beta subunit. FT /FTId=PRO_0000418873. SQ SEQUENCE 74 AA; 8752 MW; 41DD703589AAE6A0 CRC64; MSATPHTQVH WEENTARPCR KCKWQTPDPT DPLRGQCTVN RHAMGGVWKR WIRDVEHMTC SRHEEGELSF RDHV //