ID   SGR1_ARATH              Reviewed;         268 AA.
AC   O82741; Q8LBZ2;
DT   19-FEB-2014, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1998, sequence version 1.
DT   24-JUN-2015, entry version 94.
DE   RecName: Full=Protein STAY-GREEN 1, chloroplastic;
DE   AltName: Full=Protein NON-YELLOWING 1;
DE   AltName: Full=Protein STAYGREEN 1;
DE   Flags: Precursor;
GN   Name=SGR1; Synonyms=NYE1, SGN1; OrderedLocusNames=At4g22920;
GN   ORFNames=F7H19.100;
OS   Arabidopsis thaliana (Mouse-ear cress).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliophyta; eudicotyledons; Gunneridae;
OC   Pentapetalae; rosids; malvids; Brassicales; Brassicaceae; Camelineae;
OC   Arabidopsis.
OX   NCBI_TaxID=3702;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, AND DEVELOPMENTAL STAGE.
RC   STRAIN=cv. Columbia;
RX   PubMed=17513504; DOI=10.1105/tpc.106.044891;
RA   Park S.Y., Yu J.W., Park J.S., Li J., Yoo S.C., Lee N.Y., Lee S.K.,
RA   Jeong S.W., Seo H.S., Koh H.J., Jeon J.S., Park Y.I., Paek N.C.;
RT   "The senescence-induced staygreen protein regulates chlorophyll
RT   degradation.";
RL   Plant Cell 19:1649-1664(2007).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [MRNA], SUBCELLULAR LOCATION, TISSUE SPECIFICITY,
RP   INDUCTION BY DARK, AND DEVELOPMENTAL STAGE.
RC   STRAIN=cv. Columbia;
RX   PubMed=17468209; DOI=10.1104/pp.107.100172;
RA   Ren G., An K., Liao Y., Zhou X., Cao Y., Zhao H., Ge X., Kuai B.;
RT   "Identification of a novel chloroplast protein AtNYE1 regulating
RT   chlorophyll degradation during leaf senescence in Arabidopsis.";
RL   Plant Physiol. 144:1429-1441(2007).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. Columbia;
RX   PubMed=10617198; DOI=10.1038/47134;
RA   Mayer K.F.X., Schueller C., Wambutt R., Murphy G., Volckaert G.,
RA   Pohl T., Duesterhoeft A., Stiekema W., Entian K.-D., Terryn N.,
RA   Harris B., Ansorge W., Brandt P., Grivell L.A., Rieger M.,
RA   Weichselgartner M., de Simone V., Obermaier B., Mache R., Mueller M.,
RA   Kreis M., Delseny M., Puigdomenech P., Watson M., Schmidtheini T.,
RA   Reichert B., Portetelle D., Perez-Alonso M., Boutry M., Bancroft I.,
RA   Vos P., Hoheisel J., Zimmermann W., Wedler H., Ridley P.,
RA   Langham S.-A., McCullagh B., Bilham L., Robben J.,
RA   van der Schueren J., Grymonprez B., Chuang Y.-J., Vandenbussche F.,
RA   Braeken M., Weltjens I., Voet M., Bastiaens I., Aert R., Defoor E.,
RA   Weitzenegger T., Bothe G., Ramsperger U., Hilbert H., Braun M.,
RA   Holzer E., Brandt A., Peters S., van Staveren M., Dirkse W.,
RA   Mooijman P., Klein Lankhorst R., Rose M., Hauf J., Koetter P.,
RA   Berneiser S., Hempel S., Feldpausch M., Lamberth S., Van den Daele H.,
RA   De Keyser A., Buysshaert C., Gielen J., Villarroel R., De Clercq R.,
RA   van Montagu M., Rogers J., Cronin A., Quail M.A., Bray-Allen S.,
RA   Clark L., Doggett J., Hall S., Kay M., Lennard N., McLay K., Mayes R.,
RA   Pettett A., Rajandream M.A., Lyne M., Benes V., Rechmann S.,
RA   Borkova D., Bloecker H., Scharfe M., Grimm M., Loehnert T.-H.,
RA   Dose S., de Haan M., Maarse A.C., Schaefer M., Mueller-Auer S.,
RA   Gabel C., Fuchs M., Fartmann B., Granderath K., Dauner D., Herzl A.,
RA   Neumann S., Argiriou A., Vitale D., Liguori R., Piravandi E.,
RA   Massenet O., Quigley F., Clabauld G., Muendlein A., Felber R.,
RA   Schnabl S., Hiller R., Schmidt W., Lecharny A., Aubourg S.,
RA   Chefdor F., Cooke R., Berger C., Monfort A., Casacuberta E.,
RA   Gibbons T., Weber N., Vandenbol M., Bargues M., Terol J., Torres A.,
RA   Perez-Perez A., Purnelle B., Bent E., Johnson S., Tacon D., Jesse T.,
RA   Heijnen L., Schwarz S., Scholler P., Heber S., Francs P., Bielke C.,
RA   Frishman D., Haase D., Lemcke K., Mewes H.-W., Stocker S.,
RA   Zaccaria P., Bevan M., Wilson R.K., de la Bastide M., Habermann K.,
RA   Parnell L., Dedhia N., Gnoj L., Schutz K., Huang E., Spiegel L.,
RA   Sekhon M., Murray J., Sheet P., Cordes M., Abu-Threideh J.,
RA   Stoneking T., Kalicki J., Graves T., Harmon G., Edwards J.,
RA   Latreille P., Courtney L., Cloud J., Abbott A., Scott K., Johnson D.,
RA   Minx P., Bentley D., Fulton B., Miller N., Greco T., Kemp K.,
RA   Kramer J., Fulton L., Mardis E., Dante M., Pepin K., Hillier L.W.,
RA   Nelson J., Spieth J., Ryan E., Andrews S., Geisel C., Layman D.,
RA   Du H., Ali J., Berghoff A., Jones K., Drone K., Cotton M., Joshu C.,
RA   Antonoiu B., Zidanic M., Strong C., Sun H., Lamar B., Yordan C.,
RA   Ma P., Zhong J., Preston R., Vil D., Shekher M., Matero A., Shah R.,
RA   Swaby I.K., O'Shaughnessy A., Rodriguez M., Hoffman J., Till S.,
RA   Granat S., Shohdy N., Hasegawa A., Hameed A., Lodhi M., Johnson A.,
RA   Chen E., Marra M.A., Martienssen R., McCombie W.R.;
RT   "Sequence and analysis of chromosome 4 of the plant Arabidopsis
RT   thaliana.";
RL   Nature 402:769-777(1999).
RN   [4]
RP   GENOME REANNOTATION.
RC   STRAIN=cv. Columbia;
RG   The Arabidopsis Information Resource (TAIR);
RL   Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases.
RN   [5]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   STRAIN=cv. Columbia;
RX   PubMed=14593172; DOI=10.1126/science.1088305;
RA   Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J.,
RA   Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F.,
RA   Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.,
RA   Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J.,
RA   Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y.,
RA   Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P.,
RA   Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F.,
RA   Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M.,
RA   Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J.,
RA   Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T.,
RA   Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y.,
RA   Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.;
RT   "Empirical analysis of transcriptional activity in the Arabidopsis
RT   genome.";
RL   Science 302:842-846(2003).
RN   [6]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RA   Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B.,
RA   Feldmann K.A.;
RT   "Full-length cDNA from Arabidopsis thaliana.";
RL   Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases.
RN   [7]
RP   TISSUE SPECIFICITY, DEVELOPMENTAL STAGE, AND DISRUPTION PHENOTYPE.
RX   PubMed=17204643; DOI=10.1126/science.1132912;
RA   Armstead I., Donnison I., Aubry S., Harper J., Hortensteiner S.,
RA   James C., Mani J., Moffet M., Ougham H., Roberts L., Thomas A.,
RA   Weeden N., Thomas H., King I.;
RT   "Cross-species identification of Mendel's I locus.";
RL   Science 315:73-73(2007).
RN   [8]
RP   DEVELOPMENTAL STAGE, AND TISSUE SPECIFICITY.
RX   DOI=10.1016/j.plantsci.2008.12.013;
RA   Barry C.S.;
RT   "The stay-green revolution: Recent progress in deciphering the
RT   mechanisms of chlorophyll degradation in higher plants.";
RL   Plant Sci. 176:325-333(2009).
RN   [9]
RP   SUBCELLULAR LOCATION, AND INTERACTION WITH NYC1; PAO; RCCR AND LHCII
RP   COMPLEX.
RX   PubMed=22366162; DOI=10.1105/tpc.111.089474;
RA   Sakuraba Y., Schelbert S., Park S.Y., Han S.H., Lee B.D., Andres C.B.,
RA   Kessler F., Hortensteiner S., Paek N.C.;
RT   "STAY-GREEN and chlorophyll catabolic enzymes interact at light-
RT   harvesting complex II for chlorophyll detoxification during leaf
RT   senescence in Arabidopsis.";
RL   Plant Cell 24:507-518(2012).
RN   [10]
RP   INTERACTION WITH HCAR, AND DEVELOPMENTAL STAGE.
RX   PubMed=23200839; DOI=10.1016/j.bbrc.2012.11.050;
RA   Sakuraba Y., Kim Y.S., Yoo S.C., Hortensteiner S., Paek N.C.;
RT   "7-Hydroxymethyl chlorophyll a reductase functions in metabolic
RT   channeling of chlorophyll breakdown intermediates during leaf
RT   senescence.";
RL   Biochem. Biophys. Res. Commun. 430:32-37(2013).
RN   [11]
RP   FUNCTION, AND DEVELOPMENTAL STAGE.
RX   PubMed=24043799; DOI=10.1073/pnas.1308114110;
RA   Delmas F., Sankaranarayanan S., Deb S., Widdup E., Bournonville C.,
RA   Bollier N., Northey J.G., McCourt P., Samuel M.A.;
RT   "ABI3 controls embryo degreening through Mendel's I locus.";
RL   Proc. Natl. Acad. Sci. U.S.A. 110:E3888-E3894(2013).
CC   -!- FUNCTION: Involved in the disassembling mechanism of the intact
CC       light-harvesting complex of photosystem II (LHCII) in the
CC       thylakoid membranes. Required to trigger chlorophyll degradation
CC       during natural and dark-induced leaf senescence. Regulated at the
CC       transcriptional level by ABI3. {ECO:0000269|PubMed:17513504,
CC       ECO:0000269|PubMed:24043799}.
CC   -!- SUBUNIT: Interacts with HCAR, the chlorophyll catabolic enzymes
CC       (CCEs) NYC1, PAO and RCCR, and the LHCII complex. Part of a SGR1-
CC       CCE-LHCII complex, which acts in chlorophyll breakdown.
CC       {ECO:0000269|PubMed:22366162, ECO:0000269|PubMed:23200839}.
CC   -!- SUBCELLULAR LOCATION: Plastid, chloroplast thylakoid membrane
CC       {ECO:0000269|PubMed:17468209, ECO:0000269|PubMed:22366162}.
CC   -!- TISSUE SPECIFICITY: Expressed in roots, leaves, seeds, flowers,
CC       buds, petals, sepals and siliques. {ECO:0000269|PubMed:17204643,
CC       ECO:0000269|PubMed:17468209, ECO:0000269|Ref.8}.
CC   -!- DEVELOPMENTAL STAGE: Constitutively expressed at low level during
CC       leaf development, but up-regulated during seed maturation and
CC       senescence, when the leaf color changes from green to yellow.
CC       {ECO:0000269|PubMed:17204643, ECO:0000269|PubMed:17468209,
CC       ECO:0000269|PubMed:17513504, ECO:0000269|PubMed:23200839,
CC       ECO:0000269|PubMed:24043799, ECO:0000269|Ref.8}.
CC   -!- INDUCTION: Up-regulated by dark treatment.
CC       {ECO:0000269|PubMed:17468209}.
CC   -!- DISRUPTION PHENOTYPE: RNAi-mediated knockout of the protein
CC       results in a stay-green leaf phenotype. No effect on seed
CC       degreening; probably due to redundancy with SGR1. Sgr1 and sgr2
CC       double mutant has an embryo stay-green phenotype.
CC       {ECO:0000269|PubMed:17204643}.
CC   -!- SIMILARITY: Belongs to the staygreen family. {ECO:0000305}.
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DR   EMBL; AY850161; AAW82962.1; -; mRNA.
DR   EMBL; DQ437531; ABD77557.1; -; mRNA.
DR   EMBL; AL031018; CAA19807.1; -; Genomic_DNA.
DR   EMBL; AL161558; CAB79247.1; -; Genomic_DNA.
DR   EMBL; CP002687; AEE84681.1; -; Genomic_DNA.
DR   EMBL; AY063900; AAL36256.1; -; mRNA.
DR   EMBL; AY096504; AAM20154.1; -; mRNA.
DR   EMBL; AY086912; AAM64476.1; -; mRNA.
DR   PIR; T05123; T05123.
DR   RefSeq; NP_567673.1; NM_118421.3.
DR   UniGene; At.27150; -.
DR   BioGrid; 13680; 8.
DR   IntAct; O82741; 5.
DR   STRING; 3702.AT4G22920.1; -.
DR   DNASU; 828391; -.
DR   EnsemblPlants; AT4G22920.1; AT4G22920.1; AT4G22920.
DR   GeneID; 828391; -.
DR   KEGG; ath:AT4G22920; -.
DR   TAIR; AT4G22920; -.
DR   eggNOG; NOG118556; -.
DR   HOGENOM; HOG000243267; -.
DR   InParanoid; O82741; -.
DR   OMA; QRWHAAD; -.
DR   PhylomeDB; O82741; -.
DR   PRO; PR:O82741; -.
DR   Proteomes; UP000006548; Chromosome 4.
DR   GO; GO:0009535; C:chloroplast thylakoid membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0015996; P:chlorophyll catabolic process; IMP:TAIR.
DR   InterPro; IPR024438; Staygreen.
DR   Pfam; PF12638; Staygreen; 1.
PE   1: Evidence at protein level;
KW   Chloroplast; Complete proteome; Membrane; Plastid; Reference proteome;
KW   Thylakoid; Transit peptide.
FT   TRANSIT       1     48       Chloroplast. {ECO:0000255}.
FT   CHAIN        49    268       Protein STAY-GREEN 1, chloroplastic.
FT                                /FTId=PRO_0000425231.
SQ   SEQUENCE   268 AA;  30052 MW;  1B3F128F18EA79B2 CRC64;
     MCSLSAIMLL PTKLKPAYSD KRSNSSSSSS LFFNNRRSKK KNQSIVPVAR LFGPAIFESS
     KLKVLFLGVD EKKHPSTLPR TYTLTHSDIT AKLTLAISQS INNSQLQGWA NRLYRDEVVA
     EWKKVKGKMS LHVHCHISGG HFLLDLFAKF RYFIFCKELP VVLKAFVHGD GNLLNNYPEL
     QEALVWVYFH SNVNEFNKVE CWGPLWEAVS PDGHKTETLP EARCADECSC CFPTVSSIPW
     SHSLSNEGVN GYSGTQTEGI ATPNPEKL
//