ID SGR1_ARATH Reviewed; 268 AA. AC O82741; Q8LBZ2; DT 19-FEB-2014, integrated into UniProtKB/Swiss-Prot. DT 01-NOV-1998, sequence version 1. DT 03-MAY-2023, entry version 130. DE RecName: Full=Magnesium dechelatase SGR1, chloroplastic {ECO:0000305}; DE EC=4.99.1.10 {ECO:0000269|PubMed:27604697}; DE AltName: Full=Protein NONYELLOWING 1 {ECO:0000303|PubMed:17468209}; DE Short=AtNYE1 {ECO:0000303|PubMed:17468209}; DE AltName: Full=Protein STAY-GREEN 1 {ECO:0000303|PubMed:22366162}; DE AltName: Full=Protein STAYGREEN 1 {ECO:0000303|PubMed:17513504}; DE Flags: Precursor; GN Name=SGR1 {ECO:0000303|PubMed:24043799}; GN Synonyms=NYE1 {ECO:0000303|PubMed:17468209}, GN SGN1 {ECO:0000303|PubMed:17513504}; GN OrderedLocusNames=At4g22920 {ECO:0000312|Araport:AT4G22920}; GN ORFNames=F7H19.100 {ECO:0000312|EMBL:CAA19807.1}; OS Arabidopsis thaliana (Mouse-ear cress). OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae; OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis. OX NCBI_TaxID=3702; RN [1] RP NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, AND DEVELOPMENTAL STAGE. RC STRAIN=cv. Columbia; RX PubMed=17513504; DOI=10.1105/tpc.106.044891; RA Park S.Y., Yu J.W., Park J.S., Li J., Yoo S.C., Lee N.Y., Lee S.K., RA Jeong S.W., Seo H.S., Koh H.J., Jeon J.S., Park Y.I., Paek N.C.; RT "The senescence-induced staygreen protein regulates chlorophyll RT degradation."; RL Plant Cell 19:1649-1664(2007). RN [2] RP NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, SUBCELLULAR LOCATION, TISSUE RP SPECIFICITY, INDUCTION BY DARK, AND DEVELOPMENTAL STAGE. RC STRAIN=cv. Columbia; RX PubMed=17468209; DOI=10.1104/pp.107.100172; RA Ren G., An K., Liao Y., Zhou X., Cao Y., Zhao H., Ge X., Kuai B.; RT "Identification of a novel chloroplast protein AtNYE1 regulating RT chlorophyll degradation during leaf senescence in Arabidopsis."; RL Plant Physiol. 144:1429-1441(2007). RN [3] RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. RC STRAIN=cv. Columbia; RX PubMed=10617198; DOI=10.1038/47134; RA Mayer K.F.X., Schueller C., Wambutt R., Murphy G., Volckaert G., Pohl T., RA Duesterhoeft A., Stiekema W., Entian K.-D., Terryn N., Harris B., RA Ansorge W., Brandt P., Grivell L.A., Rieger M., Weichselgartner M., RA de Simone V., Obermaier B., Mache R., Mueller M., Kreis M., Delseny M., RA Puigdomenech P., Watson M., Schmidtheini T., Reichert B., Portetelle D., RA Perez-Alonso M., Boutry M., Bancroft I., Vos P., Hoheisel J., RA Zimmermann W., Wedler H., Ridley P., Langham S.-A., McCullagh B., RA Bilham L., Robben J., van der Schueren J., Grymonprez B., Chuang Y.-J., RA Vandenbussche F., Braeken M., Weltjens I., Voet M., Bastiaens I., Aert R., RA Defoor E., Weitzenegger T., Bothe G., Ramsperger U., Hilbert H., Braun M., RA Holzer E., Brandt A., Peters S., van Staveren M., Dirkse W., Mooijman P., RA Klein Lankhorst R., Rose M., Hauf J., Koetter P., Berneiser S., Hempel S., RA Feldpausch M., Lamberth S., Van den Daele H., De Keyser A., Buysshaert C., RA Gielen J., Villarroel R., De Clercq R., van Montagu M., Rogers J., RA Cronin A., Quail M.A., Bray-Allen S., Clark L., Doggett J., Hall S., RA Kay M., Lennard N., McLay K., Mayes R., Pettett A., Rajandream M.A., RA Lyne M., Benes V., Rechmann S., Borkova D., Bloecker H., Scharfe M., RA Grimm M., Loehnert T.-H., Dose S., de Haan M., Maarse A.C., Schaefer M., RA Mueller-Auer S., Gabel C., Fuchs M., Fartmann B., Granderath K., Dauner D., RA Herzl A., Neumann S., Argiriou A., Vitale D., Liguori R., Piravandi E., RA Massenet O., Quigley F., Clabauld G., Muendlein A., Felber R., Schnabl S., RA Hiller R., Schmidt W., Lecharny A., Aubourg S., Chefdor F., Cooke R., RA Berger C., Monfort A., Casacuberta E., Gibbons T., Weber N., Vandenbol M., RA Bargues M., Terol J., Torres A., Perez-Perez A., Purnelle B., Bent E., RA Johnson S., Tacon D., Jesse T., Heijnen L., Schwarz S., Scholler P., RA Heber S., Francs P., Bielke C., Frishman D., Haase D., Lemcke K., RA Mewes H.-W., Stocker S., Zaccaria P., Bevan M., Wilson R.K., RA de la Bastide M., Habermann K., Parnell L., Dedhia N., Gnoj L., Schutz K., RA Huang E., Spiegel L., Sekhon M., Murray J., Sheet P., Cordes M., RA Abu-Threideh J., Stoneking T., Kalicki J., Graves T., Harmon G., RA Edwards J., Latreille P., Courtney L., Cloud J., Abbott A., Scott K., RA Johnson D., Minx P., Bentley D., Fulton B., Miller N., Greco T., Kemp K., RA Kramer J., Fulton L., Mardis E., Dante M., Pepin K., Hillier L.W., RA Nelson J., Spieth J., Ryan E., Andrews S., Geisel C., Layman D., Du H., RA Ali J., Berghoff A., Jones K., Drone K., Cotton M., Joshu C., Antonoiu B., RA Zidanic M., Strong C., Sun H., Lamar B., Yordan C., Ma P., Zhong J., RA Preston R., Vil D., Shekher M., Matero A., Shah R., Swaby I.K., RA O'Shaughnessy A., Rodriguez M., Hoffman J., Till S., Granat S., Shohdy N., RA Hasegawa A., Hameed A., Lodhi M., Johnson A., Chen E., Marra M.A., RA Martienssen R., McCombie W.R.; RT "Sequence and analysis of chromosome 4 of the plant Arabidopsis thaliana."; RL Nature 402:769-777(1999). RN [4] RP GENOME REANNOTATION. RC STRAIN=cv. Columbia; RX PubMed=27862469; DOI=10.1111/tpj.13415; RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S., RA Town C.D.; RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference RT genome."; RL Plant J. 89:789-804(2017). RN [5] RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. RC STRAIN=cv. Columbia; RX PubMed=14593172; DOI=10.1126/science.1088305; RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L., RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., RA Ecker J.R.; RT "Empirical analysis of transcriptional activity in the Arabidopsis RT genome."; RL Science 302:842-846(2003). RN [6] RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. RA Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B., RA Feldmann K.A.; RT "Full-length cDNA from Arabidopsis thaliana."; RL Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases. RN [7] RP TISSUE SPECIFICITY, DEVELOPMENTAL STAGE, AND DISRUPTION PHENOTYPE. RX PubMed=17204643; DOI=10.1126/science.1132912; RA Armstead I., Donnison I., Aubry S., Harper J., Hortensteiner S., James C., RA Mani J., Moffet M., Ougham H., Roberts L., Thomas A., Weeden N., Thomas H., RA King I.; RT "Cross-species identification of Mendel's I locus."; RL Science 315:73-73(2007). RN [8] RP DEVELOPMENTAL STAGE, AND TISSUE SPECIFICITY. RX DOI=10.1016/j.plantsci.2008.12.013; RA Barry C.S.; RT "The stay-green revolution: Recent progress in deciphering the mechanisms RT of chlorophyll degradation in higher plants."; RL Plant Sci. 176:325-333(2009). RN [9] RP SUBCELLULAR LOCATION, AND INTERACTION WITH NYC1; PAO; RCCR AND LHCII RP COMPLEX. RX PubMed=22366162; DOI=10.1105/tpc.111.089474; RA Sakuraba Y., Schelbert S., Park S.Y., Han S.H., Lee B.D., Andres C.B., RA Kessler F., Hortensteiner S., Paek N.C.; RT "STAY-GREEN and chlorophyll catabolic enzymes interact at light-harvesting RT complex II for chlorophyll detoxification during leaf senescence in RT Arabidopsis."; RL Plant Cell 24:507-518(2012). RN [10] RP INTERACTION WITH HCAR, AND DEVELOPMENTAL STAGE. RX PubMed=23200839; DOI=10.1016/j.bbrc.2012.11.050; RA Sakuraba Y., Kim Y.S., Yoo S.C., Hortensteiner S., Paek N.C.; RT "7-Hydroxymethyl chlorophyll a reductase functions in metabolic channeling RT of chlorophyll breakdown intermediates during leaf senescence."; RL Biochem. Biophys. Res. Commun. 430:32-37(2013). RN [11] RP FUNCTION, AND DEVELOPMENTAL STAGE. RX PubMed=24043799; DOI=10.1073/pnas.1308114110; RA Delmas F., Sankaranarayanan S., Deb S., Widdup E., Bournonville C., RA Bollier N., Northey J.G., McCourt P., Samuel M.A.; RT "ABI3 controls embryo degreening through Mendel's I locus."; RL Proc. Natl. Acad. Sci. U.S.A. 110:E3888-E3894(2013). RN [12] RP INDUCTION. RX PubMed=24719469; DOI=10.1093/mp/ssu045; RA Sakuraba Y., Park S.Y., Kim Y.S., Wang S.H., Yoo S.C., Hoertensteiner S., RA Paek N.C.; RT "Arabidopsis STAY-GREEN2 is a negative regulator of chlorophyll degradation RT during leaf senescence."; RL Mol. Plant 7:1288-1302(2014). RN [13] RP FUNCTION, AND CATALYTIC ACTIVITY. RX PubMed=27604697; DOI=10.1105/tpc.16.00428; RA Shimoda Y., Ito H., Tanaka A.; RT "Arabidopsis STAY-GREEN, Mendel's green cotyledon gene, encodes magnesium- RT dechelatase."; RL Plant Cell 28:2147-2160(2016). RN [14] RP FUNCTION. RX PubMed=28873256; DOI=10.1111/tpj.13710; RA Li Z., Wu S., Chen J., Wang X., Gao J., Ren G., Kuai B.; RT "NYEs/SGRs-mediated chlorophyll degradation is critical for detoxification RT during seed maturation in Arabidopsis."; RL Plant J. 92:650-661(2017). RN [15] RP FUNCTION. RX PubMed=29425814; DOI=10.1016/j.jplph.2018.01.010; RA Sato T., Shimoda Y., Matsuda K., Tanaka A., Ito H.; RT "Mg-dechelation of chlorophyll a by Stay-Green activates chlorophyll b RT degradation through expressing Non-Yellow Coloring 1 in Arabidopsis RT thaliana."; RL J. Plant Physiol. 222:94-102(2018). CC -!- FUNCTION: Magnesium chelatase involved in chlorophyll a degradation in CC the chlorophyll-protein complexes of photosystem I (PSI) and CC photosystem II (PSII) (PubMed:27604697). Contributes to the degradation CC of PSI and PSII in the thylakoid membranes (PubMed:27604697). Required CC to trigger chlorophyll degradation during natural and dark-induced leaf CC senescence (Probable) (PubMed:17468209). Mediates chlorophyll CC degradation during embryo degreening (PubMed:24043799, CC PubMed:28873256). Recombinant SGR1 possesses high dechelating activity CC against chlorophyll a, very low activity against chlorophyllide a, and CC no activity against chlorophyll b (PubMed:27604697). Magnesium CC dechelation of chlorophyll a by SGR1 activates chlorophyll b CC degradation by inducing the expression of NYC1, an enzyme involved in CC chlorophyll b degradation (PubMed:29425814). CC {ECO:0000269|PubMed:17468209, ECO:0000269|PubMed:24043799, CC ECO:0000269|PubMed:27604697, ECO:0000269|PubMed:28873256, CC ECO:0000269|PubMed:29425814, ECO:0000305|PubMed:17513504}. CC -!- CATALYTIC ACTIVITY: CC Reaction=chlorophyll a + 2 H(+) = Mg(2+) + pheophytin a; CC Xref=Rhea:RHEA:52788, ChEBI:CHEBI:15378, ChEBI:CHEBI:18420, CC ChEBI:CHEBI:58416, ChEBI:CHEBI:136840; EC=4.99.1.10; CC Evidence={ECO:0000269|PubMed:27604697}; CC -!- SUBUNIT: Interacts with HCAR, the chlorophyll catabolic enzymes (CCEs) CC NYC1, PAO and RCCR, and the LHCII complex. Part of a SGR1-CCE-LHCII CC complex, which acts in chlorophyll breakdown. CC {ECO:0000269|PubMed:22366162, ECO:0000269|PubMed:23200839}. CC -!- SUBCELLULAR LOCATION: Plastid, chloroplast thylakoid membrane CC {ECO:0000269|PubMed:17468209, ECO:0000269|PubMed:22366162}. CC -!- TISSUE SPECIFICITY: Expressed in roots, leaves, seeds, flowers, buds, CC petals, sepals and siliques. {ECO:0000269|PubMed:17204643, CC ECO:0000269|PubMed:17468209, ECO:0000269|Ref.8}. CC -!- DEVELOPMENTAL STAGE: Constitutively expressed at low level during leaf CC development, but up-regulated during seed maturation and senescence, CC when the leaf color changes from green to yellow. CC {ECO:0000269|PubMed:17204643, ECO:0000269|PubMed:17468209, CC ECO:0000269|PubMed:17513504, ECO:0000269|PubMed:23200839, CC ECO:0000269|PubMed:24043799, ECO:0000269|Ref.8}. CC -!- INDUCTION: Up-regulated by dark treatment (PubMed:17468209). Induced CC during natural and dark-induced leaf senescence (PubMed:24719469). CC {ECO:0000269|PubMed:17468209, ECO:0000269|PubMed:24719469}. CC -!- DISRUPTION PHENOTYPE: RNAi-mediated knockout of the protein results in CC a stay-green leaf phenotype. No effect on seed degreening; probably due CC to redundancy with SGR1. Sgr1 and sgr2 double mutant has an embryo CC stay-green phenotype. {ECO:0000269|PubMed:17204643}. CC -!- SIMILARITY: Belongs to the staygreen family. {ECO:0000305}. CC --------------------------------------------------------------------------- CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms CC Distributed under the Creative Commons Attribution (CC BY 4.0) License CC --------------------------------------------------------------------------- DR EMBL; AY850161; AAW82962.1; -; mRNA. DR EMBL; DQ437531; ABD77557.1; -; mRNA. DR EMBL; AL031018; CAA19807.1; -; Genomic_DNA. DR EMBL; AL161558; CAB79247.1; -; Genomic_DNA. DR EMBL; CP002687; AEE84681.1; -; Genomic_DNA. DR EMBL; CP002687; ANM67141.1; -; Genomic_DNA. DR EMBL; AY063900; AAL36256.1; -; mRNA. DR EMBL; AY096504; AAM20154.1; -; mRNA. DR EMBL; AY086912; AAM64476.1; -; mRNA. DR PIR; T05123; T05123. DR RefSeq; NP_001328989.1; NM_001341565.1. DR RefSeq; NP_567673.1; NM_118421.4. DR AlphaFoldDB; O82741; -. DR SMR; O82741; -. DR BioGRID; 13680; 9. DR IntAct; O82741; 5. DR MINT; O82741; -. DR STRING; 3702.AT4G22920.1; -. DR PaxDb; O82741; -. DR ProteomicsDB; 232633; -. DR DNASU; 828391; -. DR EnsemblPlants; AT4G22920.1; AT4G22920.1; AT4G22920. DR EnsemblPlants; AT4G22920.2; AT4G22920.2; AT4G22920. DR GeneID; 828391; -. DR Gramene; AT4G22920.1; AT4G22920.1; AT4G22920. DR Gramene; AT4G22920.2; AT4G22920.2; AT4G22920. DR KEGG; ath:AT4G22920; -. DR Araport; AT4G22920; -. DR TAIR; locus:2127153; AT4G22920. DR eggNOG; ENOG502S3TG; Eukaryota. DR HOGENOM; CLU_073517_0_0_1; -. DR InParanoid; O82741; -. DR OMA; LEGWANR; -. DR OrthoDB; 639337at2759; -. DR PhylomeDB; O82741; -. DR BioCyc; ARA:MONQT-8230; -. DR BioCyc; MetaCyc:MON-20118; -. DR PRO; PR:O82741; -. DR Proteomes; UP000006548; Chromosome 4. DR ExpressionAtlas; O82741; baseline and differential. DR Genevisible; O82741; AT. DR GO; GO:0009535; C:chloroplast thylakoid membrane; IEA:UniProtKB-SubCell. DR GO; GO:0016829; F:lyase activity; IEA:UniProtKB-KW. DR GO; GO:0015996; P:chlorophyll catabolic process; IMP:TAIR. DR InterPro; IPR024438; Staygreen. DR PANTHER; PTHR31750:SF4; LP06106P; 1. DR PANTHER; PTHR31750; PROTEIN STAY-GREEN 1, CHLOROPLASTIC-RELATED; 1. DR Pfam; PF12638; Staygreen; 1. PE 1: Evidence at protein level; KW Chlorophyll catabolism; Chloroplast; Lyase; Membrane; Plastid; KW Reference proteome; Thylakoid; Transit peptide. FT TRANSIT 1..48 FT /note="Chloroplast" FT /evidence="ECO:0000255" FT CHAIN 49..268 FT /note="Magnesium dechelatase SGR1, chloroplastic" FT /id="PRO_0000425231" SQ SEQUENCE 268 AA; 30052 MW; 1B3F128F18EA79B2 CRC64; MCSLSAIMLL PTKLKPAYSD KRSNSSSSSS LFFNNRRSKK KNQSIVPVAR LFGPAIFESS KLKVLFLGVD EKKHPSTLPR TYTLTHSDIT AKLTLAISQS INNSQLQGWA NRLYRDEVVA EWKKVKGKMS LHVHCHISGG HFLLDLFAKF RYFIFCKELP VVLKAFVHGD GNLLNNYPEL QEALVWVYFH SNVNEFNKVE CWGPLWEAVS PDGHKTETLP EARCADECSC CFPTVSSIPW SHSLSNEGVN GYSGTQTEGI ATPNPEKL //