ID SGR1_ARATH Reviewed; 268 AA. AC O82741; Q8LBZ2; DT 19-FEB-2014, integrated into UniProtKB/Swiss-Prot. DT 01-NOV-1998, sequence version 1. DT 03-JUL-2019, entry version 115. DE RecName: Full=Magnesium dechelatase SGR1, chloroplastic {ECO:0000305}; DE EC=4.99.1.10 {ECO:0000269|PubMed:27604697}; DE AltName: Full=Protein NONYELLOWING 1 {ECO:0000303|PubMed:17468209}; DE Short=AtNYE1 {ECO:0000303|PubMed:17468209}; DE AltName: Full=Protein STAY-GREEN 1 {ECO:0000303|PubMed:22366162}; DE AltName: Full=Protein STAYGREEN 1 {ECO:0000303|PubMed:17513504}; DE Flags: Precursor; GN Name=SGR1 {ECO:0000303|PubMed:24043799}; GN Synonyms=NYE1 {ECO:0000303|PubMed:17468209}, GN SGN1 {ECO:0000303|PubMed:17513504}; GN OrderedLocusNames=At4g22920 {ECO:0000312|Araport:AT4G22920}; GN ORFNames=F7H19.100 {ECO:0000312|EMBL:CAA19807.1}; OS Arabidopsis thaliana (Mouse-ear cress). OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; OC Spermatophyta; Magnoliophyta; eudicotyledons; Gunneridae; OC Pentapetalae; rosids; malvids; Brassicales; Brassicaceae; Camelineae; OC Arabidopsis. OX NCBI_TaxID=3702; RN [1] RP NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, AND DEVELOPMENTAL STAGE. RC STRAIN=cv. Columbia; RX PubMed=17513504; DOI=10.1105/tpc.106.044891; RA Park S.Y., Yu J.W., Park J.S., Li J., Yoo S.C., Lee N.Y., Lee S.K., RA Jeong S.W., Seo H.S., Koh H.J., Jeon J.S., Park Y.I., Paek N.C.; RT "The senescence-induced staygreen protein regulates chlorophyll RT degradation."; RL Plant Cell 19:1649-1664(2007). RN [2] RP NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, SUBCELLULAR LOCATION, TISSUE RP SPECIFICITY, INDUCTION BY DARK, AND DEVELOPMENTAL STAGE. RC STRAIN=cv. Columbia; RX PubMed=17468209; DOI=10.1104/pp.107.100172; RA Ren G., An K., Liao Y., Zhou X., Cao Y., Zhao H., Ge X., Kuai B.; RT "Identification of a novel chloroplast protein AtNYE1 regulating RT chlorophyll degradation during leaf senescence in Arabidopsis."; RL Plant Physiol. 144:1429-1441(2007). RN [3] RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. RC STRAIN=cv. Columbia; RX PubMed=10617198; DOI=10.1038/47134; RA Mayer K.F.X., Schueller C., Wambutt R., Murphy G., Volckaert G., RA Pohl T., Duesterhoeft A., Stiekema W., Entian K.-D., Terryn N., RA Harris B., Ansorge W., Brandt P., Grivell L.A., Rieger M., RA Weichselgartner M., de Simone V., Obermaier B., Mache R., Mueller M., RA Kreis M., Delseny M., Puigdomenech P., Watson M., Schmidtheini T., RA Reichert B., Portetelle D., Perez-Alonso M., Boutry M., Bancroft I., RA Vos P., Hoheisel J., Zimmermann W., Wedler H., Ridley P., RA Langham S.-A., McCullagh B., Bilham L., Robben J., RA van der Schueren J., Grymonprez B., Chuang Y.-J., Vandenbussche F., RA Braeken M., Weltjens I., Voet M., Bastiaens I., Aert R., Defoor E., RA Weitzenegger T., Bothe G., Ramsperger U., Hilbert H., Braun M., RA Holzer E., Brandt A., Peters S., van Staveren M., Dirkse W., RA Mooijman P., Klein Lankhorst R., Rose M., Hauf J., Koetter P., RA Berneiser S., Hempel S., Feldpausch M., Lamberth S., Van den Daele H., RA De Keyser A., Buysshaert C., Gielen J., Villarroel R., De Clercq R., RA van Montagu M., Rogers J., Cronin A., Quail M.A., Bray-Allen S., RA Clark L., Doggett J., Hall S., Kay M., Lennard N., McLay K., Mayes R., RA Pettett A., Rajandream M.A., Lyne M., Benes V., Rechmann S., RA Borkova D., Bloecker H., Scharfe M., Grimm M., Loehnert T.-H., RA Dose S., de Haan M., Maarse A.C., Schaefer M., Mueller-Auer S., RA Gabel C., Fuchs M., Fartmann B., Granderath K., Dauner D., Herzl A., RA Neumann S., Argiriou A., Vitale D., Liguori R., Piravandi E., RA Massenet O., Quigley F., Clabauld G., Muendlein A., Felber R., RA Schnabl S., Hiller R., Schmidt W., Lecharny A., Aubourg S., RA Chefdor F., Cooke R., Berger C., Monfort A., Casacuberta E., RA Gibbons T., Weber N., Vandenbol M., Bargues M., Terol J., Torres A., RA Perez-Perez A., Purnelle B., Bent E., Johnson S., Tacon D., Jesse T., RA Heijnen L., Schwarz S., Scholler P., Heber S., Francs P., Bielke C., RA Frishman D., Haase D., Lemcke K., Mewes H.-W., Stocker S., RA Zaccaria P., Bevan M., Wilson R.K., de la Bastide M., Habermann K., RA Parnell L., Dedhia N., Gnoj L., Schutz K., Huang E., Spiegel L., RA Sekhon M., Murray J., Sheet P., Cordes M., Abu-Threideh J., RA Stoneking T., Kalicki J., Graves T., Harmon G., Edwards J., RA Latreille P., Courtney L., Cloud J., Abbott A., Scott K., Johnson D., RA Minx P., Bentley D., Fulton B., Miller N., Greco T., Kemp K., RA Kramer J., Fulton L., Mardis E., Dante M., Pepin K., Hillier L.W., RA Nelson J., Spieth J., Ryan E., Andrews S., Geisel C., Layman D., RA Du H., Ali J., Berghoff A., Jones K., Drone K., Cotton M., Joshu C., RA Antonoiu B., Zidanic M., Strong C., Sun H., Lamar B., Yordan C., RA Ma P., Zhong J., Preston R., Vil D., Shekher M., Matero A., Shah R., RA Swaby I.K., O'Shaughnessy A., Rodriguez M., Hoffman J., Till S., RA Granat S., Shohdy N., Hasegawa A., Hameed A., Lodhi M., Johnson A., RA Chen E., Marra M.A., Martienssen R., McCombie W.R.; RT "Sequence and analysis of chromosome 4 of the plant Arabidopsis RT thaliana."; RL Nature 402:769-777(1999). RN [4] RP GENOME REANNOTATION. RC STRAIN=cv. Columbia; RX PubMed=27862469; DOI=10.1111/tpj.13415; RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S., RA Town C.D.; RT "Araport11: a complete reannotation of the Arabidopsis thaliana RT reference genome."; RL Plant J. 89:789-804(2017). RN [5] RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. RC STRAIN=cv. Columbia; RX PubMed=14593172; DOI=10.1126/science.1088305; RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., RA Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., RA Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G., RA Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., RA Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., RA Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., RA Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., RA Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., RA Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., RA Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., RA Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., RA Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.; RT "Empirical analysis of transcriptional activity in the Arabidopsis RT genome."; RL Science 302:842-846(2003). RN [6] RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. RA Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B., RA Feldmann K.A.; RT "Full-length cDNA from Arabidopsis thaliana."; RL Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases. RN [7] RP TISSUE SPECIFICITY, DEVELOPMENTAL STAGE, AND DISRUPTION PHENOTYPE. RX PubMed=17204643; DOI=10.1126/science.1132912; RA Armstead I., Donnison I., Aubry S., Harper J., Hortensteiner S., RA James C., Mani J., Moffet M., Ougham H., Roberts L., Thomas A., RA Weeden N., Thomas H., King I.; RT "Cross-species identification of Mendel's I locus."; RL Science 315:73-73(2007). RN [8] RP DEVELOPMENTAL STAGE, AND TISSUE SPECIFICITY. RX DOI=10.1016/j.plantsci.2008.12.013; RA Barry C.S.; RT "The stay-green revolution: Recent progress in deciphering the RT mechanisms of chlorophyll degradation in higher plants."; RL Plant Sci. 176:325-333(2009). RN [9] RP SUBCELLULAR LOCATION, AND INTERACTION WITH NYC1; PAO; RCCR AND LHCII RP COMPLEX. RX PubMed=22366162; DOI=10.1105/tpc.111.089474; RA Sakuraba Y., Schelbert S., Park S.Y., Han S.H., Lee B.D., Andres C.B., RA Kessler F., Hortensteiner S., Paek N.C.; RT "STAY-GREEN and chlorophyll catabolic enzymes interact at light- RT harvesting complex II for chlorophyll detoxification during leaf RT senescence in Arabidopsis."; RL Plant Cell 24:507-518(2012). RN [10] RP INTERACTION WITH HCAR, AND DEVELOPMENTAL STAGE. RX PubMed=23200839; DOI=10.1016/j.bbrc.2012.11.050; RA Sakuraba Y., Kim Y.S., Yoo S.C., Hortensteiner S., Paek N.C.; RT "7-Hydroxymethyl chlorophyll a reductase functions in metabolic RT channeling of chlorophyll breakdown intermediates during leaf RT senescence."; RL Biochem. Biophys. Res. Commun. 430:32-37(2013). RN [11] RP FUNCTION, AND DEVELOPMENTAL STAGE. RX PubMed=24043799; DOI=10.1073/pnas.1308114110; RA Delmas F., Sankaranarayanan S., Deb S., Widdup E., Bournonville C., RA Bollier N., Northey J.G., McCourt P., Samuel M.A.; RT "ABI3 controls embryo degreening through Mendel's I locus."; RL Proc. Natl. Acad. Sci. U.S.A. 110:E3888-E3894(2013). RN [12] RP INDUCTION. RX PubMed=24719469; DOI=10.1093/mp/ssu045; RA Sakuraba Y., Park S.Y., Kim Y.S., Wang S.H., Yoo S.C., RA Hoertensteiner S., Paek N.C.; RT "Arabidopsis STAY-GREEN2 is a negative regulator of chlorophyll RT degradation during leaf senescence."; RL Mol. Plant 7:1288-1302(2014). RN [13] RP FUNCTION, AND CATALYTIC ACTIVITY. RX PubMed=27604697; DOI=10.1105/tpc.16.00428; RA Shimoda Y., Ito H., Tanaka A.; RT "Arabidopsis STAY-GREEN, Mendel's green cotyledon gene, encodes RT magnesium-dechelatase."; RL Plant Cell 28:2147-2160(2016). RN [14] RP FUNCTION. RX PubMed=28873256; DOI=10.1111/tpj.13710; RA Li Z., Wu S., Chen J., Wang X., Gao J., Ren G., Kuai B.; RT "NYEs/SGRs-mediated chlorophyll degradation is critical for RT detoxification during seed maturation in Arabidopsis."; RL Plant J. 92:650-661(2017). RN [15] RP FUNCTION. RX PubMed=29425814; DOI=10.1016/j.jplph.2018.01.010; RA Sato T., Shimoda Y., Matsuda K., Tanaka A., Ito H.; RT "Mg-dechelation of chlorophyll a by Stay-Green activates chlorophyll b RT degradation through expressing Non-Yellow Coloring 1 in Arabidopsis RT thaliana."; RL J. Plant Physiol. 222:94-102(2018). CC -!- FUNCTION: Magnesium chelatase involved in chlorophyll a CC degradation in the chlorophyll-protein complexes of photosystem I CC (PSI) and photosystem II (PSII) (PubMed:27604697). Contributes to CC the degradation of PSI and PSII in the thylakoid membranes CC (PubMed:27604697). Required to trigger chlorophyll degradation CC during natural and dark-induced leaf senescence (Probable) CC (PubMed:17468209). Mediates chlorophyll degradation during embryo CC degreening (PubMed:24043799, PubMed:28873256). Recombinant SGR1 CC possesses high dechelating activity against chlorophyll a, very CC low activity against chlorophyllide a, and no activity against CC chlorophyll b (PubMed:27604697). Magnesium dechelation of CC chlorophyll a by SGR1 activates chlorophyll b degradation by CC inducing the expression of NYC1, an enzyme involved in chlorophyll CC b degradation (PubMed:29425814). {ECO:0000269|PubMed:17468209, CC ECO:0000269|PubMed:24043799, ECO:0000269|PubMed:27604697, CC ECO:0000269|PubMed:28873256, ECO:0000269|PubMed:29425814, CC ECO:0000305|PubMed:17513504}. CC -!- CATALYTIC ACTIVITY: CC Reaction=chlorophyll a + 2 H(+) = Mg(2+) + pheophytin a; CC Xref=Rhea:RHEA:52788, ChEBI:CHEBI:15378, ChEBI:CHEBI:18420, CC ChEBI:CHEBI:58416, ChEBI:CHEBI:136840; EC=4.99.1.10; CC Evidence={ECO:0000269|PubMed:27604697}; CC -!- SUBUNIT: Interacts with HCAR, the chlorophyll catabolic enzymes CC (CCEs) NYC1, PAO and RCCR, and the LHCII complex. Part of a SGR1- CC CCE-LHCII complex, which acts in chlorophyll breakdown. CC {ECO:0000269|PubMed:22366162, ECO:0000269|PubMed:23200839}. CC -!- SUBCELLULAR LOCATION: Plastid, chloroplast thylakoid membrane CC {ECO:0000269|PubMed:17468209, ECO:0000269|PubMed:22366162}. CC -!- TISSUE SPECIFICITY: Expressed in roots, leaves, seeds, flowers, CC buds, petals, sepals and siliques. {ECO:0000269|PubMed:17204643, CC ECO:0000269|PubMed:17468209, ECO:0000269|Ref.8}. CC -!- DEVELOPMENTAL STAGE: Constitutively expressed at low level during CC leaf development, but up-regulated during seed maturation and CC senescence, when the leaf color changes from green to yellow. CC {ECO:0000269|PubMed:17204643, ECO:0000269|PubMed:17468209, CC ECO:0000269|PubMed:17513504, ECO:0000269|PubMed:23200839, CC ECO:0000269|PubMed:24043799, ECO:0000269|Ref.8}. CC -!- INDUCTION: Up-regulated by dark treatment (PubMed:17468209). CC Induced during natural and dark-induced leaf senescence CC (PubMed:24719469). {ECO:0000269|PubMed:17468209, CC ECO:0000269|PubMed:24719469}. CC -!- DISRUPTION PHENOTYPE: RNAi-mediated knockout of the protein CC results in a stay-green leaf phenotype. No effect on seed CC degreening; probably due to redundancy with SGR1. Sgr1 and sgr2 CC double mutant has an embryo stay-green phenotype. CC {ECO:0000269|PubMed:17204643}. CC -!- SIMILARITY: Belongs to the staygreen family. {ECO:0000305}. CC --------------------------------------------------------------------------- CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms CC Distributed under the Creative Commons Attribution (CC BY 4.0) License CC --------------------------------------------------------------------------- DR EMBL; AY850161; AAW82962.1; -; mRNA. DR EMBL; DQ437531; ABD77557.1; -; mRNA. DR EMBL; AL031018; CAA19807.1; -; Genomic_DNA. DR EMBL; AL161558; CAB79247.1; -; Genomic_DNA. DR EMBL; CP002687; AEE84681.1; -; Genomic_DNA. DR EMBL; CP002687; ANM67141.1; -; Genomic_DNA. DR EMBL; AY063900; AAL36256.1; -; mRNA. DR EMBL; AY096504; AAM20154.1; -; mRNA. DR EMBL; AY086912; AAM64476.1; -; mRNA. DR PIR; T05123; T05123. DR RefSeq; NP_001328989.1; NM_001341565.1. DR RefSeq; NP_567673.1; NM_118421.4. DR BioGrid; 13680; 9. DR IntAct; O82741; 5. DR MINT; O82741; -. DR STRING; 3702.AT4G22920.1; -. DR PaxDb; O82741; -. DR PRIDE; O82741; -. DR DNASU; 828391; -. DR EnsemblPlants; AT4G22920.1; AT4G22920.1; AT4G22920. DR EnsemblPlants; AT4G22920.2; AT4G22920.2; AT4G22920. DR GeneID; 828391; -. DR Gramene; AT4G22920.1; AT4G22920.1; AT4G22920. DR Gramene; AT4G22920.2; AT4G22920.2; AT4G22920. DR KEGG; ath:AT4G22920; -. DR Araport; AT4G22920; -. DR TAIR; locus:2127153; AT4G22920. DR eggNOG; ENOG410IETA; Eukaryota. DR eggNOG; ENOG4111UAI; LUCA. DR HOGENOM; HOG000243267; -. DR InParanoid; O82741; -. DR KO; K22013; -. DR OMA; CSLSANM; -. DR OrthoDB; 1194276at2759; -. DR PhylomeDB; O82741; -. DR BioCyc; MetaCyc:MONOMER-20118; -. DR PRO; PR:O82741; -. DR Proteomes; UP000006548; Chromosome 4. DR ExpressionAtlas; O82741; baseline and differential. DR Genevisible; O82741; AT. DR GO; GO:0009535; C:chloroplast thylakoid membrane; IEA:UniProtKB-SubCell. DR GO; GO:0015996; P:chlorophyll catabolic process; IMP:TAIR. DR InterPro; IPR024438; Staygreen. DR Pfam; PF12638; Staygreen; 1. PE 1: Evidence at protein level; KW Chlorophyll catabolism; Chloroplast; Complete proteome; Lyase; KW Membrane; Plastid; Reference proteome; Thylakoid; Transit peptide. FT TRANSIT 1 48 Chloroplast. {ECO:0000255}. FT CHAIN 49 268 Magnesium dechelatase SGR1, FT chloroplastic. FT /FTId=PRO_0000425231. SQ SEQUENCE 268 AA; 30052 MW; 1B3F128F18EA79B2 CRC64; MCSLSAIMLL PTKLKPAYSD KRSNSSSSSS LFFNNRRSKK KNQSIVPVAR LFGPAIFESS KLKVLFLGVD EKKHPSTLPR TYTLTHSDIT AKLTLAISQS INNSQLQGWA NRLYRDEVVA EWKKVKGKMS LHVHCHISGG HFLLDLFAKF RYFIFCKELP VVLKAFVHGD GNLLNNYPEL QEALVWVYFH SNVNEFNKVE CWGPLWEAVS PDGHKTETLP EARCADECSC CFPTVSSIPW SHSLSNEGVN GYSGTQTEGI ATPNPEKL //