ID PIF3_ARATH Reviewed; 524 AA. AC O80536; Q9SBC5; DT 10-MAY-2002, integrated into UniProtKB/Swiss-Prot. DT 01-NOV-1998, sequence version 1. DT 13-NOV-2007, entry version 64. DE Phytochrome-interacting factor 3 (Phytochrome-associated protein 3) DE (Basic helix-loop-helix protein 8) (bHLH 8) (AtbHLH008). GN Name=BHLH8; Synonyms=PAP3, PIF3; OrderedLocusNames=At1g09530; GN ORFNames=F14J9.19; OS Arabidopsis thaliana (Mouse-ear cress). OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; OC Spermatophyta; Magnoliophyta; eudicotyledons; core eudicotyledons; OC rosids; eurosids II; Brassicales; Brassicaceae; Arabidopsis. OX NCBI_TaxID=3702; RN [1] RP NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA]. RC STRAIN=cv. Columbia; RX MEDLINE=99059501; PubMed=9845368; DOI=10.1016/S0092-8674(00)81636-0; RA Ni M., Tepperman J.M., Quail P.H.; RT "PIF3, a phytochrome-interacting factor necessary for normal RT photoinduced signal transduction, is a novel basic helix-loop-helix RT protein."; RL Cell 95:657-667(1998). RN [2] RP NUCLEOTIDE SEQUENCE [MRNA]. RC STRAIN=cv. Columbia; RA Lee J., Yi H., Shin B., Song P.-S., Choi G.; RT "Identification and characterization of three phytochrome-associated RT proteins."; RL Submitted (AUG-1998) to the EMBL/GenBank/DDBJ databases. RN [3] RP NUCLEOTIDE SEQUENCE [MRNA]. RC STRAIN=cv. Columbia; RA Schoenbohm C., Weisshaar B.; RT "Overview of the bHLH transcription factor gene family in Arabidopsis RT thaliana."; RL Submitted (APR-2000) to the EMBL/GenBank/DDBJ databases. RN [4] RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. RC STRAIN=cv. Columbia; RX MEDLINE=21016719; PubMed=11130712; DOI=10.1038/35048500; RA Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., RA White O., Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., RA Buehler E., Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., RA Chung M.K., Conn L., Conway A.B., Conway A.R., Creasy T.H., Dewar K., RA Dunn P., Etgu P., Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., RA Gill J.E., Goldsmith A.D., Haas B., Hansen N.F., Hughes B., Huizar L., RA Hunter J.L., Jenkins J., Johnson-Hopson C., Khan S., Khaykin E., RA Kim C.J., Koo H.L., Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., RA Langin-Hooper S., Lee A., Lee J.M., Lenz C.A., Li J.H., Li Y.-P., RA Lin X., Liu S.X., Liu Z.A., Luros J.S., Maiti R., Marziali A., RA Militscher J., Miranda M., Nguyen M., Nierman W.C., Osborne B.I., RA Pai G., Peterson J., Pham P.K., Rizzo M., Rooney T., Rowley D., RA Sakano H., Salzberg S.L., Schwartz J.R., Shinn P., Southwick A.M., RA Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D., RA Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., RA Wu D., Yu G., Fraser C.M., Venter J.C., Davis R.W.; RT "Sequence and analysis of chromosome 1 of the plant Arabidopsis RT thaliana."; RL Nature 408:816-820(2000). RN [5] RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. RC STRAIN=cv. Columbia; RX MEDLINE=21932900; PubMed=11910074; DOI=10.1126/science.1071006; RA Seki M., Narusaka M., Kamiya A., Ishida J., Satou M., Sakurai T., RA Nakajima M., Enju A., Akiyama K., Oono Y., Muramatsu M., RA Hayashizaki Y., Kawai J., Carninci P., Itoh M., Ishii Y., Arakawa T., RA Shibata K., Shinagawa A., Shinozaki K.; RT "Functional annotation of a full-length Arabidopsis cDNA collection."; RL Science 296:141-145(2002). RN [6] RP FUNCTION. RX MEDLINE=99394605; PubMed=10466729; DOI=10.1038/23500; RA Ni M., Tepperman J.M., Quail P.H.; RT "Binding of phytochrome B to its nuclear signalling partner PIF3 is RT reversibly induced by light."; RL Nature 400:781-784(1999). RN [7] RP FUNCTION. RX MEDLINE=20259742; PubMed=10797009; DOI=10.1126/science.288.5467.859; RA Martinez-Garcia J.F., Huq E., Quail P.H.; RT "Direct targeting of light signals to a promoter element-bound RT transcription factor."; RL Science 288:859-863(2000). RN [8] RP GENE FAMILY ORGANIZATION, AND NOMENCLATURE. RX MEDLINE=22598051; PubMed=12679534; DOI=10.1093/molbev/msg088; RA Heim M.A., Jakoby M., Werber M., Martin C., Weisshaar B., Bailey P.C.; RT "The basic helix-loop-helix transcription factor family in plants: a RT genome-wide study of protein structure and functional diversity."; RL Mol. Biol. Evol. 20:735-747(2003). CC -!- FUNCTION: Transcription factor acting positively in the CC phytochrome signaling pathway. Activates transcription by binding CC to the G box (5'-CACGTG-3'). CC -!- SUBUNIT: Homodimer (Probable). Can form a heterodimer with REP1. CC Phytochrome B binds specifically to DNA-bound PIF3, but only upon CC red light induced conversion to the Pfr form (PfrB). Reconversion CC to Pr form causes rapid dissociation. CC -!- INTERACTION: CC Self; NbExp=2; IntAct=EBI-625701, EBI-625701; CC Q9FE22:BHLH26; NbExp=1; IntAct=EBI-625701, EBI-626001; CC Q8W2F3-2:BHLH9; NbExp=1; IntAct=EBI-625701, EBI-625732; CC P14712:PHYA; NbExp=5; IntAct=EBI-625701, EBI-624446; CC P14713:PHYB; NbExp=9; IntAct=EBI-625701, EBI-300727; CC -!- SUBCELLULAR LOCATION: Nucleus. CC -!- SIMILARITY: Contains 1 basic helix-loop-helix (bHLH) domain. CC --------------------------------------------------------------------------- CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms CC Distributed under the Creative Commons Attribution (CC BY 4.0) License CC --------------------------------------------------------------------------- DR EMBL; AF100166; AAC95156.1; -; mRNA. DR EMBL; AF088280; AAC99771.1; -; mRNA. DR EMBL; AF251693; AAL55715.1; -; mRNA. DR EMBL; AC003970; AAC33213.1; -; Genomic_DNA. DR EMBL; AK117255; BAC41930.1; -; mRNA. DR PIR; H86228; H86228. DR RefSeq; NP_172424.1; -. DR RefSeq; NP_849626.1; -. DR UniGene; At.10926; -. DR HSSP; P25912; 1HLO. DR IntAct; O80536; -. DR TRANSFAC; T04492; -. DR GeneID; 837479; -. DR GenomeReviews; CT485782_GR; AT1G09530. DR KEGG; ath:AT1G09530; -. DR TAIR; At1g09530; -. DR ArrayExpress; O80536; -. DR GermOnline; AT1G09530; Arabidopsis thaliana. DR GO; GO:0042802; F:identical protein binding; IPI:IntAct. DR InterPro; IPR001092; HLH_basic. DR InterPro; IPR011598; HLH_DNA_bd. DR Gene3D; G3DSA:4.10.280.10; HLH_DNA_bd; 1. DR Pfam; PF00010; HLH; 1. DR SMART; SM00353; HLH; 1. DR PROSITE; PS50888; HLH; 1. PE 1: Evidence at protein level; KW DNA-binding; Nucleus; Phytochrome signaling pathway; Transcription; KW Transcription regulation. FT CHAIN 1 524 Phytochrome-interacting factor 3. FT /FTId=PRO_0000127428. FT DOMAIN 357 393 Helix-loop-helix motif. FT DNA_BIND 340 356 Basic motif. FT COMPBIAS 353 358 Poly-Arg. FT COMPBIAS 422 428 Poly-Ala. FT CONFLICT 15 15 E -> D (in Ref. 2). FT CONFLICT 344 344 S -> L (in Ref. 2). SQ SEQUENCE 524 AA; 56990 MW; 1044AC01D598DE7C CRC64; MPLFELFRLT KAKLESAQDR NPSPPVDEVV ELVWENGQIS TQSQSSRSRN IPPPQANSSR AREIGNGSKT TMVDEIPMSV PSLMTGLSQD DDFVPWLNHH PSLDGYCSDF LRDVSSPVTV NEQESDMAVN QTAFPLFQRR KDGNESAPAA SSSQYNGFQS HSLYGSDRAR DLPSQQTNPD RFTQTQEPLI TSNKPSLVNF SHFLRPATFA KTTNNNLHDT KEKSPQSPPN VFQTRVLGAK DSEDKVLNES VASATPKDNQ KACLISEDSC RKDQESEKAV VCSSVGSGNS LDGPSESPSL SLKRKHSNIQ DIDCHSEDVE EESGDGRKEA GPSRTGLGSK RSRSAEVHNL SERRRRDRIN EKMRALQELI PNCNKVDKAS MLDEAIEYLK SLQLQVQIMS MASGYYLPPA VMFPPGMGHY PAAAAAMAMG MGMPYAMGLP DLSRGGSSVN HGPQFQVSGM QQQPVAMGIP RVSGGGIFAG SSTIGNGSTR DLSGSKDQTT TNNNSNLKPI KRKQGSSDQF CGSS //