ID LRP4_HUMAN Reviewed; 1905 AA. AC O75096; B2RN39; Q4AC85; Q5KTZ5; DT 13-APR-2004, integrated into UniProtKB/Swiss-Prot. DT 24-NOV-2009, sequence version 4. DT 11-NOV-2015, entry version 137. DE RecName: Full=Low-density lipoprotein receptor-related protein 4; DE Short=LRP-4; DE AltName: Full=Multiple epidermal growth factor-like domains 7; DE Flags: Precursor; GN Name=LRP4; Synonyms=KIAA0816, LRP10, MEGF7; OS Homo sapiens (Human). OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; OC Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; OC Catarrhini; Hominidae; Homo. OX NCBI_TaxID=9606; RN [1] RP NUCLEOTIDE SEQUENCE [MRNA], AND VARIANTS VAL-1086; GLY-1554 AND RP GLN-1646. RC TISSUE=Brain; RA Ishikawa K., Fujimoto H., Kim D., Saeki S.; RT "Low density lipoprotein receptor-related protein 10."; RL Submitted (MAY-2002) to the EMBL/GenBank/DDBJ databases. RN [2] RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], AND VARIANTS VAL-1086; RP GLY-1554 AND GLN-1646. RC TISSUE=Brain; RX PubMed=9693030; DOI=10.1006/geno.1998.5341; RA Nakayama M., Nakajima D., Nagase T., Nomura N., Seki N., Ohara O.; RT "Identification of high-molecular-weight proteins with multiple EGF- RT like motifs by motif-trap screening."; RL Genomics 51:27-34(1998). RN [3] RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. RX PubMed=16554811; DOI=10.1038/nature04632; RA Taylor T.D., Noguchi H., Totoki Y., Toyoda A., Kuroki Y., Dewar K., RA Lloyd C., Itoh T., Takeda T., Kim D.-W., She X., Barlow K.F., RA Bloom T., Bruford E., Chang J.L., Cuomo C.A., Eichler E., RA FitzGerald M.G., Jaffe D.B., LaButti K., Nicol R., Park H.-S., RA Seaman C., Sougnez C., Yang X., Zimmer A.R., Zody M.C., Birren B.W., RA Nusbaum C., Fujiyama A., Hattori M., Rogers J., Lander E.S., RA Sakaki Y.; RT "Human chromosome 11 DNA sequence and analysis including novel gene RT identification."; RL Nature 440:497-500(2006). RN [4] RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], AND VARIANTS VAL-1086; RP GLY-1554 AND GLN-1646. RC TISSUE=Muscle; RX PubMed=15489334; DOI=10.1101/gr.2596504; RG The MGC Project Team; RT "The status, quality, and expansion of the NIH full-length cDNA RT project: the Mammalian Gene Collection (MGC)."; RL Genome Res. 14:2121-2127(2004). RN [5] RP FUNCTION IN THE REGULATION OF CANONICAL WTN SIGNALING, VARIANTS CLSS RP ASN-137; TYR-160; ASN-449; PRO-461; PHE-473; ASN-529 AND ARG-1017, AND RP CHARACTERIZATION OF VARIANTS CLSS ASN-137; TYR-160; ASN-449; PHE-473 RP AND ASN-529. RX PubMed=20381006; DOI=10.1016/j.ajhg.2010.03.004; RA Li Y., Pawlik B., Elcioglu N., Aglan M., Kayserili H., Yigit G., RA Percin F., Goodman F., Nurnberg G., Cenani A., Urquhart J., RA Chung B.D., Ismail S., Amr K., Aslanger A.D., Becker C., Netzer C., RA Scambler P., Eyaid W., Hamamy H., Clayton-Smith J., Hennekam R., RA Nurnberg P., Herz J., Temtamy S.A., Wollnik B.; RT "LRP4 mutations alter Wnt/beta-catenin signaling and cause limb and RT kidney malformations in Cenani-Lenz syndrome."; RL Am. J. Hum. Genet. 86:696-706(2010). RN [6] RP FUNCTION, INTERACTION WITH SOST, TISSUE SPECIFICITY, VARIANTS SOST2 RP TRP-1170 AND SER-1186, CHARACTERIZATION OF VARIANTS SOST2 TRP-1170 AND RP SER-1186, AND IDENTIFICATION BY MASS SPECTROMETRY. RX PubMed=21471202; DOI=10.1074/jbc.M110.190330; RA Leupin O., Piters E., Halleux C., Hu S., Kramer I., Morvan F., RA Bouwmeester T., Schirle M., Bueno-Lozano M., Fuentes F.J., Itin P.H., RA Boudin E., de Freitas F., Jennes K., Brannetti B., Charara N., RA Ebersbach H., Geisse S., Lu C.X., Bauer A., Van Hul W., Kneissel M.; RT "Bone overgrowth-associated mutations in the LRP4 gene impair RT sclerostin facilitator function."; RL J. Biol. Chem. 286:19489-19500(2011). RN [7] RP INVOLVEMENT IN CMS17, VARIANTS SOST2 TRP-1170 AND SER-1186, VARIANTS RP CMS17 LYS-1233 AND HIS-1277, CHARACTERIZATION OF VARIANTS SOST2 RP TRP-1170 AND SER-1186, CHARACTERIZATION OF VARIANTS CMS17 LYS-1233 AND RP HIS-1277, AND MUTAGENESIS OF ASN-1214; VAL-1252; TYR-1256 AND RP ILE-1287. RX PubMed=24234652; DOI=10.1093/hmg/ddt578; RA Ohkawara B., Cabrera-Serrano M., Nakata T., Milone M., Asai N., RA Ito K., Ito M., Masuda A., Ito Y., Engel A.G., Ohno K.; RT "LRP4 third beta-propeller domain mutations cause novel congenital RT myasthenia by compromising agrin-mediated MuSK signaling in a RT position-specific manner."; RL Hum. Mol. Genet. 23:1856-1868(2014). CC -!- FUNCTION: Mediates SOST-dependent inhibition of bone formation. CC Functions as a specific facilitator of SOST-mediated inhibition of CC Wnt signaling. Plays a key role in the formation and the CC maintenance of the neuromuscular junction (NMJ), the synapse CC between motor neuron and skeletal muscle. Directly binds AGRIN and CC recruits it to the MUSK signaling complex. Mediates the AGRIN- CC induced phosphorylation of MUSK, the kinase of the complex. The CC activation of MUSK in myotubes induces the formation of NMJ by CC regulating different processes including the transcription of CC specific genes and the clustering of AChR in the postsynaptic CC membrane. Alternatively, may be involved in the negative CC regulation of the canonical Wnt signaling pathway, being able to CC antagonize the LRP6-mediated activation of this pathway. More CC generally, has been proposed to function as a cell surface CC endocytic receptor binding and internalizing extracellular ligands CC for degradation by lysosomes. {ECO:0000269|PubMed:20381006, CC ECO:0000269|PubMed:21471202}. CC -!- SUBUNIT: Homooligomer. Interacts with MUSK; the heterodimer forms CC an AGRIN receptor complex that binds AGRIN resulting in activation CC of MUSK (By similarity). Interacts (via the extracellular domain) CC with SOST; the interaction facilitates the inhibition of Wnt CC signaling. {ECO:0000250, ECO:0000269|PubMed:21471202}. CC -!- INTERACTION: CC Q9BQB4:SOST; NbExp=4; IntAct=EBI-310873, EBI-5746563; CC -!- SUBCELLULAR LOCATION: Membrane {ECO:0000305}; Single-pass type I CC membrane protein {ECO:0000305}. CC -!- TISSUE SPECIFICITY: Expressed in bone; present in osteoblasts and CC osteocytes. No expression is observed in osteoclast. Expressed in CC several regions of the brain. {ECO:0000269|PubMed:21471202}. CC -!- DISEASE: Cenani-Lenz syndactyly syndrome (CLSS) [MIM:212780]: A CC congenital malformation syndrome defined as complete and complex CC syndactyly of the hands combined with malformations of the forearm CC bones and similar manifestations in the lower limbs. It is CC characterized by fusion and disorganization of metacarpal and CC phalangeal bones, radius and ulnar shortening, radioulnar CC synostosis, and severe syndactyly of hands and feet. CC {ECO:0000269|PubMed:20381006}. Note=The disease is caused by CC mutations affecting the gene represented in this entry. CC -!- DISEASE: Sclerosteosis 2 (SOST2) [MIM:614305]: A sclerosing bone CC dysplasia characterized by a generalized hyperostosis and CC sclerosis leading to a markedly thickened skull, with mandible, CC ribs, clavicles and all long bones also being affected. Due to CC narrowing of the foramina of the cranial nerves, facial nerve CC palsy, hearing loss and atrophy of the optic nerves can occur. CC Sclerosteosis is clinically and radiologically very similar to van CC Buchem disease, mainly differentiated by hand malformations and a CC large stature in sclerosteosis patients. CC {ECO:0000269|PubMed:21471202, ECO:0000269|PubMed:24234652}. CC Note=The disease is caused by mutations affecting the gene CC represented in this entry. CC -!- DISEASE: Myasthenic syndrome, congenital, 17 (CMS17) [MIM:616304]: CC A form of congenital myasthenic syndrome, a group of disorders CC characterized by failure of neuromuscular transmission, including CC pre-synaptic, synaptic, and post-synaptic disorders that are not CC of autoimmune origin. Clinical features are easy fatigability and CC muscle weakness affecting the axial and limb muscles (with CC hypotonia in early-onset forms), the ocular muscles (leading to CC ptosis and ophthalmoplegia), and the facial and bulbar musculature CC (affecting sucking and swallowing, and leading to dysphonia). The CC symptoms fluctuate and worsen with physical effort. CC {ECO:0000269|PubMed:24234652}. Note=The disease is caused by CC mutations affecting the gene represented in this entry. CC -!- SIMILARITY: Belongs to the LDLR family. {ECO:0000305}. CC -!- SIMILARITY: Contains 3 EGF-like domains. {ECO:0000305}. CC -!- SIMILARITY: Contains 8 LDL-receptor class A domains. CC {ECO:0000255|PROSITE-ProRule:PRU00124}. CC -!- SIMILARITY: Contains 20 LDL-receptor class B repeats. CC {ECO:0000255|PROSITE-ProRule:PRU00461}. CC -!- SEQUENCE CAUTION: CC Sequence=BAE19679.1; Type=Erroneous initiation; Note=Translation N-terminally shortened.; Evidence={ECO:0000305}; CC --------------------------------------------------------------------------- CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms CC Distributed under the Creative Commons Attribution (CC BY 4.0) License CC --------------------------------------------------------------------------- DR EMBL; AB084910; BAD83615.1; -; mRNA. DR EMBL; AB011540; BAA32468.1; -; mRNA. DR EMBL; AB231861; BAE19679.1; ALT_INIT; mRNA. DR EMBL; AC021573; -; NOT_ANNOTATED_CDS; Genomic_DNA. DR EMBL; BC037360; AAH37360.1; -; mRNA. DR EMBL; BC041048; AAH41048.1; -; mRNA. DR EMBL; BC136667; AAI36668.1; -; mRNA. DR EMBL; BC136668; AAI36669.1; -; mRNA. DR CCDS; CCDS31478.1; -. DR RefSeq; NP_002325.2; NM_002334.3. DR UniGene; Hs.4930; -. DR ProteinModelPortal; O75096; -. DR SMR; O75096; 27-1640. DR BioGrid; 110218; 24. DR IntAct; O75096; 4. DR STRING; 9606.ENSP00000367888; -. DR PhosphoSite; O75096; -. DR BioMuta; LRP4; -. DR MaxQB; O75096; -. DR PaxDb; O75096; -. DR PRIDE; O75096; -. DR Ensembl; ENST00000378623; ENSP00000367888; ENSG00000134569. DR GeneID; 4038; -. DR KEGG; hsa:4038; -. DR UCSC; uc001ndn.4; human. DR CTD; 4038; -. DR GeneCards; LRP4; -. DR H-InvDB; HIX0009607; -. DR HGNC; HGNC:6696; LRP4. DR HPA; HPA011934; -. DR HPA; HPA012300; -. DR MIM; 212780; phenotype. DR MIM; 604270; gene. DR MIM; 614305; phenotype. DR MIM; 616304; phenotype. DR neXtProt; NX_O75096; -. DR Orphanet; 3258; Cenani-Lenz syndrome. DR Orphanet; 98913; Postsynaptic congenital myasthenic syndromes. DR Orphanet; 3152; Sclerosteosis. DR PharmGKB; PA30454; -. DR eggNOG; KOG1215; Eukaryota. DR eggNOG; ENOG410XPR2; LUCA. DR GeneTree; ENSGT00760000118968; -. DR HOGENOM; HOG000047507; -. DR HOVERGEN; HBG049163; -. DR InParanoid; O75096; -. DR OMA; GSQLLWA; -. DR OrthoDB; EOG75XGK3; -. DR PhylomeDB; O75096; -. DR TreeFam; TF315253; -. DR Reactome; R-HSA-3000178; ECM proteoglycans. DR ChiTaRS; LRP4; human. DR GeneWiki; Low_density_lipoprotein_receptor-related_protein_4; -. DR GenomeRNAi; 4038; -. DR NextBio; 15818; -. DR PRO; PR:O75096; -. DR Proteomes; UP000005640; Chromosome 11. DR Bgee; O75096; -. DR CleanEx; HS_LRP4; -. DR ExpressionAtlas; O75096; baseline and differential. DR Genevisible; O75096; HS. DR GO; GO:0009986; C:cell surface; IDA:UniProtKB. DR GO; GO:0030425; C:dendrite; ISS:UniProtKB. DR GO; GO:0016600; C:flotillin complex; IEA:Ensembl. DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW. DR GO; GO:0031594; C:neuromuscular junction; ISS:UniProtKB. DR GO; GO:0043025; C:neuronal cell body; ISS:UniProtKB. DR GO; GO:0014069; C:postsynaptic density; ISS:UniProtKB. DR GO; GO:0097060; C:synaptic membrane; ISS:UniProtKB. DR GO; GO:0005509; F:calcium ion binding; IEA:InterPro. DR GO; GO:0030971; F:receptor tyrosine kinase binding; ISS:UniProtKB. DR GO; GO:0097110; F:scaffold protein binding; ISS:UniProtKB. DR GO; GO:0042813; F:Wnt-activated receptor activity; IBA:GO_Central. DR GO; GO:0017147; F:Wnt-protein binding; IBA:GO_Central. DR GO; GO:0060070; P:canonical Wnt signaling pathway; IBA:GO_Central. DR GO; GO:0048813; P:dendrite morphogenesis; ISS:UniProtKB. DR GO; GO:0042733; P:embryonic digit morphogenesis; IEA:Ensembl. DR GO; GO:0006897; P:endocytosis; IEA:UniProtKB-KW. DR GO; GO:0030198; P:extracellular matrix organization; TAS:Reactome. DR GO; GO:0001942; P:hair follicle development; IEA:Ensembl. DR GO; GO:0001822; P:kidney development; IDA:UniProtKB. DR GO; GO:0060173; P:limb development; IDA:UniProtKB. DR GO; GO:0050771; P:negative regulation of axonogenesis; ISS:UniProtKB. DR GO; GO:0090090; P:negative regulation of canonical Wnt signaling pathway; IMP:UniProtKB. DR GO; GO:0030279; P:negative regulation of ossification; IMP:UniProtKB. DR GO; GO:0042475; P:odontogenesis of dentin-containing tooth; IEA:Ensembl. DR GO; GO:1901631; P:positive regulation of presynaptic membrane organization; ISS:UniProtKB. DR GO; GO:0097104; P:postsynaptic membrane assembly; ISS:UniProtKB. DR GO; GO:0097105; P:presynaptic membrane assembly; ISS:UniProtKB. DR GO; GO:0051290; P:protein heterotetramerization; ISS:UniProtKB. DR GO; GO:0009954; P:proximal/distal pattern formation; IEA:Ensembl. DR GO; GO:0001932; P:regulation of protein phosphorylation; IEA:Ensembl. DR GO; GO:0071340; P:skeletal muscle acetylcholine-gated channel clustering; ISS:UniProtKB. DR GO; GO:0050808; P:synapse organization; ISS:UniProtKB. DR GO; GO:0051124; P:synaptic growth at neuromuscular junction; ISS:UniProtKB. DR GO; GO:0044332; P:Wnt signaling pathway involved in dorsal/ventral axis specification; IBA:GO_Central. DR Gene3D; 2.120.10.30; -; 4. DR Gene3D; 4.10.400.10; -; 8. DR InterPro; IPR011042; 6-blade_b-propeller_TolB-like. DR InterPro; IPR026823; cEGF. DR InterPro; IPR001881; EGF-like_Ca-bd_dom. DR InterPro; IPR013032; EGF-like_CS. DR InterPro; IPR000742; EGF-like_dom. DR InterPro; IPR000152; EGF-type_Asp/Asn_hydroxyl_site. DR InterPro; IPR018097; EGF_Ca-bd_CS. DR InterPro; IPR009030; Growth_fac_rcpt_N_dom. DR InterPro; IPR023415; LDLR_class-A_CS. DR InterPro; IPR000033; LDLR_classB_rpt. DR InterPro; IPR002172; LDrepeatLR_classA_rpt. DR InterPro; IPR030799; LRP4. DR PANTHER; PTHR10529:SF249; PTHR10529:SF249; 4. DR Pfam; PF12662; cEGF; 1. DR Pfam; PF00057; Ldl_recept_a; 8. DR Pfam; PF00058; Ldl_recept_b; 16. DR PRINTS; PR00261; LDLRECEPTOR. DR SMART; SM00181; EGF; 4. DR SMART; SM00179; EGF_CA; 1. DR SMART; SM00192; LDLa; 8. DR SMART; SM00135; LY; 20. DR SUPFAM; SSF57184; SSF57184; 2. DR SUPFAM; SSF57424; SSF57424; 8. DR PROSITE; PS00010; ASX_HYDROXYL; 1. DR PROSITE; PS01186; EGF_2; 3. DR PROSITE; PS01187; EGF_CA; 1. DR PROSITE; PS01209; LDLRA_1; 8. DR PROSITE; PS50068; LDLRA_2; 8. DR PROSITE; PS51120; LDLRB; 20. PE 1: Evidence at protein level; KW Calcium; Complete proteome; Congenital myasthenic syndrome; KW Developmental protein; Differentiation; Disease mutation; KW Disulfide bond; EGF-like domain; Endocytosis; Glycoprotein; Membrane; KW Polymorphism; Receptor; Reference proteome; Repeat; Signal; KW Transmembrane; Transmembrane helix; Wnt signaling pathway. FT SIGNAL 1 20 {ECO:0000255}. FT CHAIN 21 1905 Low-density lipoprotein receptor-related FT protein 4. FT /FTId=PRO_0000017325. FT TOPO_DOM 21 1725 Extracellular. {ECO:0000255}. FT TRANSMEM 1726 1746 Helical. {ECO:0000255}. FT TOPO_DOM 1747 1905 Cytoplasmic. {ECO:0000255}. FT DOMAIN 26 67 LDL-receptor class A 1. FT {ECO:0000255|PROSITE-ProRule:PRU00124}. FT DOMAIN 70 106 LDL-receptor class A 2. FT {ECO:0000255|PROSITE-ProRule:PRU00124}. FT DOMAIN 109 144 LDL-receptor class A 3. FT {ECO:0000255|PROSITE-ProRule:PRU00124}. FT DOMAIN 147 183 LDL-receptor class A 4. FT {ECO:0000255|PROSITE-ProRule:PRU00124}. FT DOMAIN 190 226 LDL-receptor class A 5. FT {ECO:0000255|PROSITE-ProRule:PRU00124}. FT DOMAIN 230 266 LDL-receptor class A 6. FT {ECO:0000255|PROSITE-ProRule:PRU00124}. FT DOMAIN 269 305 LDL-receptor class A 7. FT {ECO:0000255|PROSITE-ProRule:PRU00124}. FT DOMAIN 311 350 LDL-receptor class A 8. FT {ECO:0000255|PROSITE-ProRule:PRU00124}. FT DOMAIN 354 394 EGF-like 1; calcium-binding. FT {ECO:0000255}. FT DOMAIN 395 434 EGF-like 2; calcium-binding. FT {ECO:0000255}. FT REPEAT 480 522 LDL-receptor class B 1. FT REPEAT 523 565 LDL-receptor class B 2. FT REPEAT 566 609 LDL-receptor class B 3. FT REPEAT 610 652 LDL-receptor class B 4. FT REPEAT 653 693 LDL-receptor class B 5. FT DOMAIN 698 737 EGF-like 3. FT REPEAT 785 827 LDL-receptor class B 6. FT REPEAT 828 870 LDL-receptor class B 7. FT REPEAT 871 914 LDL-receptor class B 8. FT REPEAT 915 956 LDL-receptor class B 9. FT REPEAT 957 998 LDL-receptor class B 10. FT REPEAT 1093 1135 LDL-receptor class B 11. FT REPEAT 1136 1178 LDL-receptor class B 12. FT REPEAT 1179 1222 LDL-receptor class B 13. FT REPEAT 1223 1263 LDL-receptor class B 14. FT REPEAT 1264 1306 LDL-receptor class B 15. FT REPEAT 1397 1439 LDL-receptor class B 16. FT REPEAT 1440 1482 LDL-receptor class B 17. FT REPEAT 1483 1526 LDL-receptor class B 18. FT REPEAT 1527 1568 LDL-receptor class B 19. FT REPEAT 1569 1610 LDL-receptor class B 20. FT MOTIF 1766 1769 Endocytosis signal. {ECO:0000255}. FT CARBOHYD 264 264 N-linked (GlcNAc...). {ECO:0000255}. FT CARBOHYD 498 498 N-linked (GlcNAc...). {ECO:0000255}. FT CARBOHYD 719 719 N-linked (GlcNAc...). {ECO:0000255}. FT CARBOHYD 901 901 N-linked (GlcNAc...). {ECO:0000255}. FT CARBOHYD 1077 1077 N-linked (GlcNAc...). {ECO:0000255}. FT CARBOHYD 1415 1415 N-linked (GlcNAc...). {ECO:0000255}. FT CARBOHYD 1467 1467 N-linked (GlcNAc...). {ECO:0000255}. FT DISULFID 27 44 {ECO:0000255|PROSITE-ProRule:PRU00124}. FT DISULFID 34 57 {ECO:0000255|PROSITE-ProRule:PRU00124}. FT DISULFID 51 66 {ECO:0000255|PROSITE-ProRule:PRU00124}. FT DISULFID 71 83 {ECO:0000255|PROSITE-ProRule:PRU00124}. FT DISULFID 78 96 {ECO:0000255|PROSITE-ProRule:PRU00124}. FT DISULFID 90 105 {ECO:0000255|PROSITE-ProRule:PRU00124}. FT DISULFID 110 122 {ECO:0000255|PROSITE-ProRule:PRU00124}. FT DISULFID 117 135 {ECO:0000255|PROSITE-ProRule:PRU00124}. FT DISULFID 129 143 {ECO:0000255|PROSITE-ProRule:PRU00124}. FT DISULFID 148 160 {ECO:0000255|PROSITE-ProRule:PRU00124}. FT DISULFID 155 173 {ECO:0000255|PROSITE-ProRule:PRU00124}. FT DISULFID 167 182 {ECO:0000255|PROSITE-ProRule:PRU00124}. FT DISULFID 191 203 {ECO:0000255|PROSITE-ProRule:PRU00124}. FT DISULFID 198 216 {ECO:0000255|PROSITE-ProRule:PRU00124}. FT DISULFID 210 225 {ECO:0000255|PROSITE-ProRule:PRU00124}. FT DISULFID 231 243 {ECO:0000255|PROSITE-ProRule:PRU00124}. FT DISULFID 238 256 {ECO:0000255|PROSITE-ProRule:PRU00124}. FT DISULFID 250 265 {ECO:0000255|PROSITE-ProRule:PRU00124}. FT DISULFID 270 282 {ECO:0000255|PROSITE-ProRule:PRU00124}. FT DISULFID 277 295 {ECO:0000255|PROSITE-ProRule:PRU00124}. FT DISULFID 289 304 {ECO:0000255|PROSITE-ProRule:PRU00124}. FT DISULFID 312 324 {ECO:0000255|PROSITE-ProRule:PRU00124}. FT DISULFID 319 337 {ECO:0000255|PROSITE-ProRule:PRU00124}. FT DISULFID 331 349 {ECO:0000255|PROSITE-ProRule:PRU00124}. FT DISULFID 358 369 {ECO:0000255|PROSITE-ProRule:PRU00124}. FT DISULFID 365 378 {ECO:0000255|PROSITE-ProRule:PRU00124}. FT DISULFID 380 393 {ECO:0000255|PROSITE-ProRule:PRU00124}. FT DISULFID 399 409 {ECO:0000255|PROSITE-ProRule:PRU00124}. FT DISULFID 405 418 {ECO:0000255|PROSITE-ProRule:PRU00124}. FT DISULFID 420 433 {ECO:0000255|PROSITE-ProRule:PRU00124}. FT DISULFID 702 713 {ECO:0000255|PROSITE-ProRule:PRU00124}. FT DISULFID 709 722 {ECO:0000255|PROSITE-ProRule:PRU00124}. FT DISULFID 724 736 {ECO:0000255|PROSITE-ProRule:PRU00124}. FT VARIANT 137 137 D -> N (in CLSS; abolishes the FT antagonistic effect of LRP4 on LRP6- FT mediated activation of Wnt signaling). FT {ECO:0000269|PubMed:20381006}. FT /FTId=VAR_063776. FT VARIANT 160 160 C -> Y (in CLSS; abolishes the FT antagonistic effect of LRP4 on LRP6- FT mediated activation of Wnt signaling). FT {ECO:0000269|PubMed:20381006}. FT /FTId=VAR_063777. FT VARIANT 314 314 L -> S (in dbSNP:rs7926667). FT /FTId=VAR_058290. FT VARIANT 449 449 D -> N (in CLSS; abolishes the FT antagonistic effect of LRP4 on LRP6- FT mediated activation of Wnt signaling). FT {ECO:0000269|PubMed:20381006}. FT /FTId=VAR_063778. FT VARIANT 461 461 T -> P (in CLSS). FT {ECO:0000269|PubMed:20381006}. FT /FTId=VAR_063779. FT VARIANT 473 473 L -> F (in CLSS; abolishes the FT antagonistic effect of LRP4 on LRP6- FT mediated activation of Wnt signaling). FT {ECO:0000269|PubMed:20381006}. FT /FTId=VAR_063780. FT VARIANT 529 529 D -> N (in CLSS; abolishes the FT antagonistic effect of LRP4 on LRP6- FT mediated activation of Wnt signaling). FT {ECO:0000269|PubMed:20381006}. FT /FTId=VAR_063781. FT VARIANT 1017 1017 C -> R (in CLSS). FT {ECO:0000269|PubMed:20381006}. FT /FTId=VAR_063782. FT VARIANT 1086 1086 I -> V (in dbSNP:rs6485702). FT {ECO:0000269|PubMed:15489334, FT ECO:0000269|PubMed:9693030, FT ECO:0000269|Ref.1}. FT /FTId=VAR_057955. FT VARIANT 1170 1170 R -> W (in SOST2; impairs the interaction FT with SOST; loss of function as FT facilitator of SOST-mediated inhibition FT of Wnt signaling; has no effect on AGRN- FT mediated MUSK signaling; retains the FT ability to bind AGRN and MUSK). FT {ECO:0000269|PubMed:21471202, FT ECO:0000269|PubMed:24234652}. FT /FTId=VAR_066630. FT VARIANT 1186 1186 W -> S (in SOST2; impairs the interaction FT with SOST; loss of function as FT facilitator of SOST-mediated inhibition FT of Wnt signaling; has no effect on AGRN- FT mediated MUSK signaling; retains the FT ability to bind AGRN and MUSK). FT {ECO:0000269|PubMed:21471202, FT ECO:0000269|PubMed:24234652}. FT /FTId=VAR_066631. FT VARIANT 1203 1203 A -> V (in dbSNP:rs2306033). FT /FTId=VAR_058291. FT VARIANT 1233 1233 E -> K (in CMS17; decreases binding FT affinity for AGRN and MUSK proteins; does FT not enhance downstream activation of the FT MUSK signaling pathway thus impairing FT clustering of AChRs). FT {ECO:0000269|PubMed:24234652}. FT /FTId=VAR_073695. FT VARIANT 1238 1238 A -> T (in dbSNP:rs2306031). FT /FTId=VAR_058292. FT VARIANT 1277 1277 R -> H (in CMS17; decreases binding FT affinity for AGRN and MUSK proteins; does FT not enhance downstream activation of the FT MUSK signaling pathway thus impairing FT clustering of AChRs). FT {ECO:0000269|PubMed:24234652}. FT /FTId=VAR_073696. FT VARIANT 1554 1554 S -> G (in dbSNP:rs2306029). FT {ECO:0000269|PubMed:15489334, FT ECO:0000269|PubMed:9693030, FT ECO:0000269|Ref.1}. FT /FTId=VAR_057956. FT VARIANT 1646 1646 R -> Q (in dbSNP:rs3816614). FT {ECO:0000269|PubMed:15489334, FT ECO:0000269|PubMed:9693030, FT ECO:0000269|Ref.1}. FT /FTId=VAR_057957. FT MUTAGEN 1214 1214 N->A: Compromises Wnt-suppressive FT activity. {ECO:0000269|PubMed:24234652}. FT MUTAGEN 1252 1252 V->A: Compromises AGRN-mediated up- FT regulation of MUSK signaling. FT {ECO:0000269|PubMed:24234652}. FT MUTAGEN 1256 1256 Y->A: Compromises Wnt-suppressive FT activity. {ECO:0000269|PubMed:24234652}. FT MUTAGEN 1287 1287 I->A: Compromises AGRN-mediated up- FT regulation of MUSK signaling. FT {ECO:0000269|PubMed:24234652}. FT CONFLICT 1472 1475 EPRA -> D (in Ref. 2; BAE19679). FT {ECO:0000305}. FT CONFLICT 1478 1478 V -> A (in Ref. 2; BAE19679). FT {ECO:0000305}. FT CONFLICT 1862 1862 T -> M (in Ref. 1; BAD83615 and 2; FT BAA32468). {ECO:0000305}. SQ SEQUENCE 1905 AA; 212045 MW; A39117C18279F9AA CRC64; MRRQWGALLL GALLCAHGLA SSPECACGRS HFTCAVSALG ECTCIPAQWQ CDGDNDCGDH SDEDGCILPT CSPLDFHCDN GKCIRRSWVC DGDNDCEDDS DEQDCPPREC EEDEFPCQNG YCIRSLWHCD GDNDCGDNSD EQCDMRKCSD KEFRCSDGSC IAEHWYCDGD TDCKDGSDEE NCPSAVPAPP CNLEEFQCAY GRCILDIYHC DGDDDCGDWS DESDCSSHQP CRSGEFMCDS GLCINAGWRC DGDADCDDQS DERNCTTSMC TAEQFRCHSG RCVRLSWRCD GEDDCADNSD EENCENTGSP QCALDQFLCW NGRCIGQRKL CNGVNDCGDN SDESPQQNCR PRTGEENCNV NNGGCAQKCQ MVRGAVQCTC HTGYRLTEDG HTCQDVNECA EEGYCSQGCT NSEGAFQCWC ETGYELRPDR RSCKALGPEP VLLFANRIDI RQVLPHRSEY TLLLNNLENA IALDFHHRRE LVFWSDVTLD RILRANLNGS NVEEVVSTGL ESPGGLAVDW VHDKLYWTDS GTSRIEVANL DGAHRKVLLW QNLEKPRAIA LHPMEGTIYW TDWGNTPRIE ASSMDGSGRR IIADTHLFWP NGLTIDYAGR RMYWVDAKHH VIERANLDGS HRKAVISQGL PHPFAITVFE DSLYWTDWHT KSINSANKFT GKNQEIIRNK LHFPMDIHTL HPQRQPAGKN RCGDNNGGCT HLCLPSGQNY TCACPTGFRK ISSHACAQSL DKFLLFARRM DIRRISFDTE DLSDDVIPLA DVRSAVALDW DSRDDHVYWT DVSTDTISRA KWDGTGQEVV VDTSLESPAG LAIDWVTNKL YWTDAGTDRI EVANTDGSMR TVLIWENLDR PRDIVVEPMG GYMYWTDWGA SPKIERAGMD ASGRQVIISS NLTWPNGLAI DYGSQRLYWA DAGMKTIEFA GLDGSKRKVL IGSQLPHPFG LTLYGERIYW TDWQTKSIQS ADRLTGLDRE TLQENLENLM DIHVFHRRRP PVSTPCAMEN GGCSHLCLRS PNPSGFSCTC PTGINLLSDG KTCSPGMNSF LIFARRIDIR MVSLDIPYFA DVVVPINITM KNTIAIGVDP QEGKVYWSDS TLHRISRANL DGSQHEDIIT TGLQTTDGLA VDAIGRKVYW TDTGTNRIEV GNLDGSMRKV LVWQNLDSPR AIVLYHEMGF MYWTDWGENA KLERSGMDGS DRAVLINNNL GWPNGLTVDK ASSQLLWADA HTERIEAADL NGANRHTLVS PVQHPYGLTL LDSYIYWTDW QTRSIHRADK GTGSNVILVR SNLPGLMDMQ AVDRAQPLGF NKCGSRNGGC SHLCLPRPSG FSCACPTGIQ LKGDGKTCDP SPETYLLFSS RGSIRRISLD TSDHTDVHVP VPELNNVISL DYDSVDGKVY YTDVFLDVIR RADLNGSNME TVIGRGLKTT DGLAVDWVAR NLYWTDTGRN TIEASRLDGS CRKVLINNSL DEPRAIAVFP RKGYLFWTDW GHIAKIERAN LDGSERKVLI NTDLGWPNGL TLDYDTRRIY WVDAHLDRIE SADLNGKLRQ VLVSHVSHPF ALTQQDRWIY WTDWQTKSIQ RVDKYSGRNK ETVLANVEGL MDIIVVSPQR QTGTNACGVN NGGCTHLCFA RASDFVCACP DEPDSRPCSL VPGLVPPAPR ATGMSEKSPV LPNTPPTTLY SSTTRTRTSL EEVEGRCSER DARLGLCARS NDAVPAAPGE GLHISYAIGG LLSILLILVV IAALMLYRHK KSKFTDPGMG NLTYSNPSYR TSTQEVKIEA IPKPAMYNQL CYKKEGGPDH NYTKEKIKIV EGICLLSGDD AEWDDLKQLR SSRGGLLRDH VCMKTDTVSI QASSGSLDDT ETEQLLQEEQ SECSSVHTAA TPERRGSLPD TGWKHERKLS SESQV //