ID R1A_GLRV3 Reviewed; 2233 AA. AC O71188; DT 30-NOV-2010, integrated into UniProtKB/Swiss-Prot. DT 01-JUN-2003, sequence version 2. DT 13-SEP-2023, entry version 89. DE RecName: Full=Replicase protein 1a; DE Contains: DE RecName: Full=Leader protease; DE Short=L-Pro; DE EC=3.4.22.-; DE AltName: Full=Papain-like cysteine proteinase; DE Short=PCP; DE Contains: DE RecName: Full=Methyltransferase/helicase; DE EC=2.1.1.-; DE EC=3.6.4.13; GN ORFNames=1a; OS Grapevine leafroll-associated virus 3 (isolate United States/NY1) (GLRaV-3) OS (Grapevine leafroll-associated closterovirus (isolate 109)). OC Viruses; Riboviria; Orthornavirae; Kitrinoviricota; Alsuviricetes; OC Martellivirales; Closteroviridae; Ampelovirus. OX NCBI_TaxID=651354; OH NCBI_TaxID=29760; Vitis vinifera (Grape). RN [1] RP NUCLEOTIDE SEQUENCE [GENOMIC RNA]. RX PubMed=9603346; DOI=10.1099/0022-1317-79-5-1299; RA Ling K.S., Zhu H.Y., Drong R.F., Slightom J.L., McFerson J.R., RA Gonsalves D.; RT "Nucleotide sequence of the 3'-terminal two-thirds of the grapevine RT leafroll-associated virus-3 genome reveals a typical monopartite RT closterovirus."; RL J. Gen. Virol. 79:1299-1307(1998). CC -!- CATALYTIC ACTIVITY: CC Reaction=ATP + H2O = ADP + H(+) + phosphate; Xref=Rhea:RHEA:13065, CC ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616, CC ChEBI:CHEBI:43474, ChEBI:CHEBI:456216; EC=3.6.4.13; CC -!- COFACTOR: CC Name=Fe(2+); Xref=ChEBI:CHEBI:29033; CC Evidence={ECO:0000255|PROSITE-ProRule:PRU00805}; CC Note=Binds 1 Fe(2+) ion per subunit. {ECO:0000255|PROSITE- CC ProRule:PRU00805}; CC -!- ALTERNATIVE PRODUCTS: CC Event=Ribosomal frameshifting; Named isoforms=2; CC Comment=The replicase 1a is produced from conventional translation of CC the 1a ORF. The replicase 1ab is generated probably by a +1 ribosomal CC frameshifting mechanism occurring at the 1a-1b genes boundary.; CC Name=Replicase 1a; CC IsoId=O71188-1; Sequence=Displayed; CC Name=Replicase 1ab; CC IsoId=O71189-1; Sequence=External; CC --------------------------------------------------------------------------- CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms CC Distributed under the Creative Commons Attribution (CC BY 4.0) License CC --------------------------------------------------------------------------- DR EMBL; AF037268; AAC40717.3; -; Genomic_RNA. DR RefSeq; NP_813795.3; NC_004667.1. DR SMR; O71188; -. DR GeneID; 1444473; -. DR KEGG; vg:1444473; -. DR Proteomes; UP000006707; Segment. DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW. DR GO; GO:0016887; F:ATP hydrolysis activity; IEA:RHEA. DR GO; GO:0008234; F:cysteine-type peptidase activity; IEA:UniProtKB-KW. DR GO; GO:0051213; F:dioxygenase activity; IEA:UniProtKB-KW. DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW. DR GO; GO:0008174; F:mRNA methyltransferase activity; IEA:InterPro. DR GO; GO:0003723; F:RNA binding; IEA:InterPro. DR GO; GO:0003724; F:RNA helicase activity; IEA:UniProtKB-EC. DR GO; GO:0006508; P:proteolysis; IEA:UniProtKB-KW. DR GO; GO:0006396; P:RNA processing; IEA:InterPro. DR Gene3D; 2.60.120.590; Alpha-ketoglutarate-dependent dioxygenase AlkB-like; 1. DR Gene3D; 3.40.50.300; P-loop containing nucleotide triphosphate hydrolases; 2. DR InterPro; IPR027351; (+)RNA_virus_helicase_core_dom. DR InterPro; IPR027450; AlkB-like. DR InterPro; IPR037151; AlkB-like_sf. DR InterPro; IPR002588; Alphavirus-like_MT_dom. DR InterPro; IPR005123; Oxoglu/Fe-dep_dioxygenase. DR InterPro; IPR027417; P-loop_NTPase. DR Pfam; PF13532; 2OG-FeII_Oxy_2; 1. DR Pfam; PF01443; Viral_helicase1; 1. DR Pfam; PF01660; Vmethyltransf; 1. DR SUPFAM; SSF51197; Clavaminate synthase-like; 1. DR SUPFAM; SSF52540; P-loop containing nucleoside triphosphate hydrolases; 2. DR PROSITE; PS51743; ALPHAVIRUS_MT; 1. DR PROSITE; PS51471; FE2OG_OXY; 1. DR PROSITE; PS51657; PSRV_HELICASE; 1. PE 3: Inferred from homology; KW ATP-binding; Dioxygenase; Helicase; Hydrolase; Iron; Metal-binding; KW Methyltransferase; Nucleotide-binding; Oxidoreductase; Protease; KW Reference proteome; Ribosomal frameshifting; Thiol protease; Transferase. FT CHAIN 1..2233 FT /note="Replicase protein 1a" FT /id="PRO_0000402531" FT CHAIN 1..393 FT /note="Leader protease" FT /evidence="ECO:0000250" FT /id="PRO_0000402532" FT CHAIN 394..2233 FT /note="Methyltransferase/helicase" FT /evidence="ECO:0000250" FT /id="PRO_0000402533" FT DOMAIN 478..669 FT /note="Alphavirus-like MT" FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01079" FT DOMAIN 1601..1694 FT /note="Fe2OG dioxygenase" FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00805" FT DOMAIN 1902..2066 FT /note="(+)RNA virus helicase ATP-binding" FT DOMAIN 2067..2230 FT /note="(+)RNA virus helicase C-terminal" FT REGION 143..163 FT /note="Disordered" FT /evidence="ECO:0000256|SAM:MobiDB-lite" FT REGION 1277..1301 FT /note="Disordered" FT /evidence="ECO:0000256|SAM:MobiDB-lite" FT REGION 1400..1442 FT /note="Disordered" FT /evidence="ECO:0000256|SAM:MobiDB-lite" FT REGION 1708..1746 FT /note="Disordered" FT /evidence="ECO:0000256|SAM:MobiDB-lite" FT COMPBIAS 146..161 FT /note="Basic and acidic residues" FT /evidence="ECO:0000256|SAM:MobiDB-lite" FT COMPBIAS 1412..1442 FT /note="Polar residues" FT /evidence="ECO:0000256|SAM:MobiDB-lite" FT COMPBIAS 1714..1737 FT /note="Polar residues" FT /evidence="ECO:0000256|SAM:MobiDB-lite" FT ACT_SITE 311 FT /note="For leader protease activity" FT /evidence="ECO:0000250" FT ACT_SITE 357 FT /note="For leader protease activity" FT /evidence="ECO:0000250" FT BINDING 1619 FT /ligand="Fe cation" FT /ligand_id="ChEBI:CHEBI:24875" FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00805" FT BINDING 1621 FT /ligand="Fe cation" FT /ligand_id="ChEBI:CHEBI:24875" FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00805" FT BINDING 1676 FT /ligand="Fe cation" FT /ligand_id="ChEBI:CHEBI:24875" FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00805" FT BINDING 1685 FT /ligand="2-oxoglutarate" FT /ligand_id="ChEBI:CHEBI:16810" FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00805" FT SITE 393..394 FT /note="Cleavage; by the leader protease" FT /evidence="ECO:0000250" SQ SEQUENCE 2233 AA; 244804 MW; CDDC18B6133FB08C CRC64; MDYIRPLRVF SFPHVNNTLE YVRYNKANGD VGAFLTTMKF IGNVKLSDFT PRCAAMIYIG KLTKGVKRTF VPPPVKGFAR QYAVVSGSVS ALRGDGKKVL MEARTSTSAT SDVSDFDVVF EAVSNALLVV HYHRVVPYAP VKREQPKPAV KQDEQKPKRQ ASHWAVKPTA VGVHVPLPKK QEALEPAQSV PQQSLEEKAA LTFGLFFSKG GGDESDAVIL RKGKLFNRAL NVPIDVKNTF VWAKIWDEAS RRRGYFYVKD RAVKFFPIVR GRATIEDFIV NTAPGCDVAL PRIELWSMRE RAFVCTTKGW CWFNNERLRG EIYRRRCFSS SFSIGFLMHL GFRSLKVIRF AGTNILHMPS LNEERTFGWK GGDVYLPNVP KTAIVAGDRT RLGGEILASV ANALNQEEVY SSVVSSITNR LVLRDQSALL SHLDTKLCDM FSQRDAMIRE KPSHRCDVFL KPREREKLRE LFPELSIQFS DSVRSSHPFA NAMRSCFNGI FSRRCGNVCF FDIGGSFTYH VKAGHVNCHV CNPVLDVKDV KRRINEILFL STAGGDSYVS SDLLTEAASK SVSYCSRESQ NCDSRADAGF MVDVYDISPQ QVAEAMDKKG ALVFDIALMF PVELLYGNGE VYLEELDTLV KREGDYLAYN VGQCGEMYEH SFSNVSGFFT FSYVRTSSGN VFKLEYEGYR CGYHHLTMCR AQKSPGTEVT YRSLVPSFVG KSLVFIPVVA GSSVSFKTIV LDSDFVDRIY SYALNTIGTF ENRTFEYAVG AVRSQKTHVI TGSRVVHSKV DISPDDMWGL VVAVMAQAIK DRAKSIRSYN FIKASEGSLA GVFKLFFQTV GDCFSNAVSV YAKAMVHDNF NVLETLMSMP RAFIRKVPGS VVVTICTSGA SDRLELRGAF DISKETFGRK LKNSRLRVFS RAIVEDSIKV MKAMKTEDGK PLPITEDSVY AFIMGNVSNV HCTRAGLLGG SKATVVSSVS KGLVARGAAT KAFSGITSFF STGSLFYDRG LTEDERLDAL VRTENAINSP VGILETSRVA VSKVVAGTKE FWSEVSLNDF TTFVLRNKVL IGIFVASLGA APIAWKYRRG IAANARRYAG SSYETLSSLS SQAAGGLRGL TSSTVSGGSL VVRRGFSSAV TVTRATVAKR QVPLALLSFS TSYAISGCSM LGIWAHALPR HLMFFFGLGT LLGARASANT WKFGGFSNNW CAVPEVVWRG KSVSSLLLPI TLGVSLIIRG LLNDTIPQLA YVPPVEGRNV YDETLRYYRD FDYDEGAGPS GTQHEAVPGD DNDGSTSSVS SYDVVTNVRD VGISTNGEVT GEEETHSPRS VQYTYVEEEV APSAAVAERQ GDPSGSGTAD AMAFVESVKK GVDDVFHQQS SGETAREVEV DGKGLLPESV VGEAPTQERG RAADGNTAQT AVNEGDREPV QSSLVSSPQA DIPKVTQSEV HAQKEVKQEV PLATVSGATP IVDEKPAPSV TTRGVKIIDK GKAVAHVAEK KQVQVEQPKQ RSLTINEGKA GKQLCMFRTC SCGVQLDVYN EATIATRFSN AFTFVDNLKG RSAVFFSKLG EGYTYNGGSH VSSGWPRALE DILTAIKYPS VFDHCLVQKY KMGGGVPFHA DDEECYPSDN PILTVNLVGK ANFSTKCRKG GKVMVINVAS GDYFLMPCGF QRTHLHSVNS IDEGRISLTF RATRRVFGVG RMLQLAGGVS DEKSPGVPNQ QPQSQGATRT ITPKSGGKAL SEGSGREVKG RSTYSIWCEQ DYVRKCEWLR ADNPVMALEP DYTPMTFEVV KTGTSEDAVV EYLKYLAIGI ERTYRALLMA RNIAVTTAEG VLKVPNQVYE SLPGFHVYKS GTDLIFHSTQ DGLRVRDLPY VLIAEKGIFT KGKDVDAVVA LGDNLFVCDD ILVFHDAINL IGALKVARCG MVGESFKSFE YKCYNAPPGG GKTTTLVDEF VKSPNSTATI TANVGSSEDI NMAVKKRDPN LEGLNSATTV NSRVVNFIVR GMYKRVLVDE VHMMHQGLLQ LGVFATGASE GLFFGDINQI PFINREKVFR MDCAVFVPKK ESVVYTSKSY RCPLDVCYLL SSMTVRGTEK CYPEKVVSGK DKPVVRSLSK RPIGTTDDVA EINADVYLCM TQLEKSDMKR SLKGKGKETP VMTVHEAQGK TFSDVVLFRT KKADDSLFTK QPHILVGLSR HTRSLVYAAL SSKLDDKVGT YISDASPQSV SDALLHTFAP AGC //