ID R1A_GLRV3 Reviewed; 2233 AA. AC O71188; DT 30-NOV-2010, integrated into UniProtKB/Swiss-Prot. DT 01-JUN-2003, sequence version 2. DT 01-APR-2015, entry version 64. DE RecName: Full=Replicase protein 1a; DE Contains: DE RecName: Full=Leader protease; DE Short=L-Pro; DE EC=3.4.22.-; DE AltName: Full=Papain-like cysteine proteinase; DE Short=PCP; DE Contains: DE RecName: Full=Methyltransferase/helicase; DE EC=2.1.1.-; DE EC=3.6.4.13; GN ORFNames=1a; OS Grapevine leafroll-associated virus 3 (isolate United States/NY1) OS (GLRaV-3) (Grapevine leafroll-associated closterovirus (isolate 109)). OC Viruses; ssRNA positive-strand viruses, no DNA stage; Closteroviridae; OC Ampelovirus. OX NCBI_TaxID=651354; OH NCBI_TaxID=29760; Vitis vinifera (Grape). RN [1] RP NUCLEOTIDE SEQUENCE [GENOMIC RNA]. RX PubMed=9603346; RA Ling K.S., Zhu H.Y., Drong R.F., Slightom J.L., McFerson J.R., RA Gonsalves D.; RT "Nucleotide sequence of the 3'-terminal two-thirds of the grapevine RT leafroll-associated virus-3 genome reveals a typical monopartite RT closterovirus."; RL J. Gen. Virol. 79:1299-1307(1998). CC -!- CATALYTIC ACTIVITY: ATP + H(2)O = ADP + phosphate. CC -!- COFACTOR: CC Name=Fe(2+); Xref=ChEBI:CHEBI:29033; CC Evidence={ECO:0000255|PROSITE-ProRule:PRU00805}; CC Note=Binds 1 Fe(2+) ion per subunit. {ECO:0000255|PROSITE- CC ProRule:PRU00805}; CC -!- ALTERNATIVE PRODUCTS: CC Event=Ribosomal frameshifting; Named isoforms=2; CC Comment=The replicase 1a is produced from conventional CC translation of the 1a ORF. The replicase 1ab is generated CC probably by a +1 ribosomal frameshifting mechanism occurring at CC the 1a-1b genes boundary.; CC Name=Replicase 1a; CC IsoId=O71188-1; Sequence=Displayed; CC Name=Replicase 1ab; CC IsoId=O71189-1; Sequence=External; CC -!- SIMILARITY: Contains 1 (+)RNA virus helicase ATP-binding domain. CC {ECO:0000305}. CC -!- SIMILARITY: Contains 1 (+)RNA virus helicase C-terminal domain. CC {ECO:0000305}. CC -!- SIMILARITY: Contains 1 alphavirus-like MT (methyltransferase) CC domain. {ECO:0000255|PROSITE-ProRule:PRU01079}. CC -!- SIMILARITY: Contains 1 Fe2OG dioxygenase domain. CC {ECO:0000255|PROSITE-ProRule:PRU00805}. CC --------------------------------------------------------------------------- CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms CC Distributed under the Creative Commons Attribution (CC BY 4.0) License CC --------------------------------------------------------------------------- DR EMBL; AF037268; AAC40717.3; -; Genomic_RNA. DR RefSeq; NP_813795.3; NC_004667.1. DR GeneID; 1444473; -. DR KEGG; vg:1444473; -. DR Proteomes; UP000006707; Genome. DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW. DR GO; GO:0008234; F:cysteine-type peptidase activity; IEA:UniProtKB-KW. DR GO; GO:0004386; F:helicase activity; IEA:UniProtKB-KW. DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW. DR GO; GO:0008174; F:mRNA methyltransferase activity; IEA:InterPro. DR GO; GO:0016706; F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, 2-oxoglutarate as one donor, and incorporation of one atom each of oxygen into both donors; IEA:InterPro. DR GO; GO:0003723; F:RNA binding; IEA:InterPro. DR GO; GO:0006396; P:RNA processing; IEA:InterPro. DR Gene3D; 2.60.120.590; -; 1. DR Gene3D; 3.40.50.300; -; 1. DR InterPro; IPR027351; (+)RNA_virus_helicase_core_dom. DR InterPro; IPR027450; AlkB-like. DR InterPro; IPR005123; Oxoglu/Fe-dep_dioxygenase. DR InterPro; IPR027417; P-loop_NTPase. DR InterPro; IPR002588; Tymovirus_MeTrfase. DR Pfam; PF13532; 2OG-FeII_Oxy_2; 1. DR Pfam; PF01443; Viral_helicase1; 1. DR Pfam; PF01660; Vmethyltransf; 1. DR SUPFAM; SSF52540; SSF52540; 3. DR PROSITE; PS51743; ALPHAVIRUS_MT; 1. DR PROSITE; PS51471; FE2OG_OXY; 1. DR PROSITE; PS51657; PSRV_HELICASE; 1. PE 3: Inferred from homology; KW ATP-binding; Complete proteome; Dioxygenase; Helicase; Hydrolase; KW Iron; Metal-binding; Methyltransferase; Nucleotide-binding; KW Oxidoreductase; Protease; Reference proteome; Ribosomal frameshifting; KW Thiol protease; Transferase. FT CHAIN 1 2233 Replicase protein 1a. FT /FTId=PRO_0000402531. FT CHAIN 1 393 Leader protease. {ECO:0000250}. FT /FTId=PRO_0000402532. FT CHAIN 394 2233 Methyltransferase/helicase. FT {ECO:0000250}. FT /FTId=PRO_0000402533. FT DOMAIN 478 669 Alphavirus-like MT. {ECO:0000255|PROSITE- FT ProRule:PRU01079}. FT DOMAIN 1601 1694 Fe2OG dioxygenase. {ECO:0000255|PROSITE- FT ProRule:PRU00805}. FT DOMAIN 1902 2066 (+)RNA virus helicase ATP-binding. FT DOMAIN 2067 2230 (+)RNA virus helicase C-terminal. FT ACT_SITE 311 311 For leader protease activity. FT {ECO:0000250}. FT ACT_SITE 357 357 For leader protease activity. FT {ECO:0000250}. FT METAL 1619 1619 Iron. {ECO:0000255|PROSITE- FT ProRule:PRU00805}. FT METAL 1621 1621 Iron. {ECO:0000255|PROSITE- FT ProRule:PRU00805}. FT METAL 1676 1676 Iron. {ECO:0000255|PROSITE- FT ProRule:PRU00805}. FT BINDING 1685 1685 2-oxoglutarate. {ECO:0000255|PROSITE- FT ProRule:PRU00805}. FT SITE 393 394 Cleavage; by the leader protease. FT {ECO:0000250}. SQ SEQUENCE 2233 AA; 244804 MW; CDDC18B6133FB08C CRC64; MDYIRPLRVF SFPHVNNTLE YVRYNKANGD VGAFLTTMKF IGNVKLSDFT PRCAAMIYIG KLTKGVKRTF VPPPVKGFAR QYAVVSGSVS ALRGDGKKVL MEARTSTSAT SDVSDFDVVF EAVSNALLVV HYHRVVPYAP VKREQPKPAV KQDEQKPKRQ ASHWAVKPTA VGVHVPLPKK QEALEPAQSV PQQSLEEKAA LTFGLFFSKG GGDESDAVIL RKGKLFNRAL NVPIDVKNTF VWAKIWDEAS RRRGYFYVKD RAVKFFPIVR GRATIEDFIV NTAPGCDVAL PRIELWSMRE RAFVCTTKGW CWFNNERLRG EIYRRRCFSS SFSIGFLMHL GFRSLKVIRF AGTNILHMPS LNEERTFGWK GGDVYLPNVP KTAIVAGDRT RLGGEILASV ANALNQEEVY SSVVSSITNR LVLRDQSALL SHLDTKLCDM FSQRDAMIRE KPSHRCDVFL KPREREKLRE LFPELSIQFS DSVRSSHPFA NAMRSCFNGI FSRRCGNVCF FDIGGSFTYH VKAGHVNCHV CNPVLDVKDV KRRINEILFL STAGGDSYVS SDLLTEAASK SVSYCSRESQ NCDSRADAGF MVDVYDISPQ QVAEAMDKKG ALVFDIALMF PVELLYGNGE VYLEELDTLV KREGDYLAYN VGQCGEMYEH SFSNVSGFFT FSYVRTSSGN VFKLEYEGYR CGYHHLTMCR AQKSPGTEVT YRSLVPSFVG KSLVFIPVVA GSSVSFKTIV LDSDFVDRIY SYALNTIGTF ENRTFEYAVG AVRSQKTHVI TGSRVVHSKV DISPDDMWGL VVAVMAQAIK DRAKSIRSYN FIKASEGSLA GVFKLFFQTV GDCFSNAVSV YAKAMVHDNF NVLETLMSMP RAFIRKVPGS VVVTICTSGA SDRLELRGAF DISKETFGRK LKNSRLRVFS RAIVEDSIKV MKAMKTEDGK PLPITEDSVY AFIMGNVSNV HCTRAGLLGG SKATVVSSVS KGLVARGAAT KAFSGITSFF STGSLFYDRG LTEDERLDAL VRTENAINSP VGILETSRVA VSKVVAGTKE FWSEVSLNDF TTFVLRNKVL IGIFVASLGA APIAWKYRRG IAANARRYAG SSYETLSSLS SQAAGGLRGL TSSTVSGGSL VVRRGFSSAV TVTRATVAKR QVPLALLSFS TSYAISGCSM LGIWAHALPR HLMFFFGLGT LLGARASANT WKFGGFSNNW CAVPEVVWRG KSVSSLLLPI TLGVSLIIRG LLNDTIPQLA YVPPVEGRNV YDETLRYYRD FDYDEGAGPS GTQHEAVPGD DNDGSTSSVS SYDVVTNVRD VGISTNGEVT GEEETHSPRS VQYTYVEEEV APSAAVAERQ GDPSGSGTAD AMAFVESVKK GVDDVFHQQS SGETAREVEV DGKGLLPESV VGEAPTQERG RAADGNTAQT AVNEGDREPV QSSLVSSPQA DIPKVTQSEV HAQKEVKQEV PLATVSGATP IVDEKPAPSV TTRGVKIIDK GKAVAHVAEK KQVQVEQPKQ RSLTINEGKA GKQLCMFRTC SCGVQLDVYN EATIATRFSN AFTFVDNLKG RSAVFFSKLG EGYTYNGGSH VSSGWPRALE DILTAIKYPS VFDHCLVQKY KMGGGVPFHA DDEECYPSDN PILTVNLVGK ANFSTKCRKG GKVMVINVAS GDYFLMPCGF QRTHLHSVNS IDEGRISLTF RATRRVFGVG RMLQLAGGVS DEKSPGVPNQ QPQSQGATRT ITPKSGGKAL SEGSGREVKG RSTYSIWCEQ DYVRKCEWLR ADNPVMALEP DYTPMTFEVV KTGTSEDAVV EYLKYLAIGI ERTYRALLMA RNIAVTTAEG VLKVPNQVYE SLPGFHVYKS GTDLIFHSTQ DGLRVRDLPY VLIAEKGIFT KGKDVDAVVA LGDNLFVCDD ILVFHDAINL IGALKVARCG MVGESFKSFE YKCYNAPPGG GKTTTLVDEF VKSPNSTATI TANVGSSEDI NMAVKKRDPN LEGLNSATTV NSRVVNFIVR GMYKRVLVDE VHMMHQGLLQ LGVFATGASE GLFFGDINQI PFINREKVFR MDCAVFVPKK ESVVYTSKSY RCPLDVCYLL SSMTVRGTEK CYPEKVVSGK DKPVVRSLSK RPIGTTDDVA EINADVYLCM TQLEKSDMKR SLKGKGKETP VMTVHEAQGK TFSDVVLFRT KKADDSLFTK QPHILVGLSR HTRSLVYAAL SSKLDDKVGT YISDASPQSV SDALLHTFAP AGC //