ID NKG2D_RAT Reviewed; 215 AA. AC O70215; DT 07-JUN-2004, integrated into UniProtKB/Swiss-Prot. DT 01-AUG-1998, sequence version 1. DT 18-APR-2012, entry version 75. DE RecName: Full=NKG2-D type II integral membrane protein; DE AltName: Full=Killer cell lectin-like receptor subfamily K member 1; DE AltName: Full=NK cell receptor D; DE AltName: Full=NK lectin-like receptor; DE Short=NKLLR; DE AltName: Full=NKG2-D-activating NK receptor; DE AltName: Full=NKR-P2; DE AltName: CD_antigen=CD314; GN Name=Klrk1; Synonyms=Nkg2d, Nkrp2; OS Rattus norvegicus (Rat). OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; OC Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Sciurognathi; OC Muroidea; Muridae; Murinae; Rattus. OX NCBI_TaxID=10116; RN [1] RP NUCLEOTIDE SEQUENCE [MRNA], AND TISSUE SPECIFICITY. RC STRAIN=Fischer 344; TISSUE=Natural killer cell; RX MEDLINE=98281903; PubMed=9620593; DOI=10.1093/intimm/10.4.379; RA Berg S.F., Dissen E., Westgaard I.H., Fossum S.; RT "Molecular characterization of rat NKR-P2, a lectin-like receptor RT expressed by NK cells and resting T cells."; RL Int. Immunol. 10:379-385(1998). RN [2] RP TISSUE SPECIFICITY. RX PubMed=15048723; DOI=10.1002/eji.200324793; RA Alli R., Savithri B., Das S., Varalakshmi C., Rangaraj N., Khar A.; RT "Involvement of NKR-P2/NKG2D in DC-mediated killing of tumor targets: RT indicative of a common, innate, target-recognition paradigm?"; RL Eur. J. Immunol. 34:1119-1126(2004). CC -!- FUNCTION: Involved in the immune surveillance exerted by T and B CC lymphocytes. Functions as a natural cytotoxicity receptor to CC stimulate perforin-mediated elimination of ligand-expressing tumor CC cells. Signaling involves calcium influx, culminating in the CC expression of TNF-alpha. CC -!- SUBUNIT: Homodimer. Interacts with DAP10. The interaction with CC DAP10 is required for NKG2D cell surface expression (By CC similarity). CC -!- SUBCELLULAR LOCATION: Membrane; Single-pass type II membrane CC protein (By similarity). CC -!- TISSUE SPECIFICITY: Expressed by natural killer cells and by CC resting thoracic duct CD4+ and CD8+ T-cells, but not by thymocytes CC or other hemopoietic cells. Expressed by DC cells. CC -!- SIMILARITY: Contains 1 C-type lectin domain. CC --------------------------------------------------------------------------- CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms CC Distributed under the Creative Commons Attribution (CC BY 4.0) License CC --------------------------------------------------------------------------- DR EMBL; AF009511; AAC40092.1; -; mRNA. DR IPI; IPI00206939; -. DR RefSeq; NP_598196.1; NM_133512.1. DR UniGene; Rn.14544; -. DR ProteinModelPortal; O70215; -. DR SMR; O70215; 93-215. DR STRING; O70215; -. DR PRIDE; O70215; -. DR Ensembl; ENSRNOT00000012886; ENSRNOP00000012886; ENSRNOG00000009638. DR GeneID; 24934; -. DR KEGG; rno:24934; -. DR UCSC; NM_133512; rat. DR CTD; 22914; -. DR RGD; 3180; Klrk1. DR eggNOG; NOG315914; -. DR GeneTree; ENSGT00640000091112; -. DR HOVERGEN; HBG052629; -. DR KO; K06728; -. DR OrthoDB; EOG49KFRM; -. DR NextBio; 604911; -. DR ArrayExpress; O70215; -. DR Genevestigator; O70215; -. DR GermOnline; ENSRNOG00000009638; Rattus norvegicus. DR GO; GO:0016021; C:integral to membrane; IEA:UniProtKB-KW. DR GO; GO:0005529; F:sugar binding; IEA:UniProtKB-KW. DR Gene3D; G3DSA:3.10.100.10; C-type_lectin-like; 1. DR InterPro; IPR002353; AntifreezeII. DR InterPro; IPR001304; C-type_lectin. DR InterPro; IPR016186; C-type_lectin-like. DR InterPro; IPR016187; C-type_lectin_fold. DR Pfam; PF00059; Lectin_C; 1. DR PRINTS; PR00356; ANTIFREEZEII. DR SMART; SM00034; CLECT; 1. DR SUPFAM; SSF56436; C-type_lectin_fold; 1. DR PROSITE; PS00615; C_TYPE_LECTIN_1; FALSE_NEG. DR PROSITE; PS50041; C_TYPE_LECTIN_2; 1. PE 2: Evidence at transcript level; KW Complete proteome; Disulfide bond; Glycoprotein; Lectin; Membrane; KW Receptor; Reference proteome; Signal-anchor; Transmembrane; KW Transmembrane helix. FT CHAIN 1 215 NKG2-D type II integral membrane protein. FT /FTId=PRO_0000046670. FT TOPO_DOM 1 51 Cytoplasmic (Potential). FT TRANSMEM 52 74 Helical; Signal-anchor for type II FT membrane protein; (Potential). FT TOPO_DOM 75 215 Extracellular (Potential). FT DOMAIN 105 211 C-type lectin. FT CARBOHYD 120 120 N-linked (GlcNAc...) (Potential). FT CARBOHYD 130 130 N-linked (GlcNAc...) (Potential). FT CARBOHYD 162 162 N-linked (GlcNAc...) (Potential). FT DISULFID 95 104 By similarity. FT DISULFID 98 109 By similarity. FT DISULFID 126 210 By similarity. FT DISULFID 188 202 By similarity. SQ SEQUENCE 215 AA; 24438 MW; B49C0364613031AF CRC64; MSKCHNYDLK PAKWDTSQEH QKQRSALPTS RPGENGIIRR RSSIEELKIS PLFVVRVLVA AMTIRFTVIT LTWLAVFITL LCNKEVSVSS REGYCGPCPN DWICHRNNCY QFFNENKAWN QSQASCLSQN SSLLKIYSKE EQDFLKLVKS YHWMGLVQSP ANGSWQWEDG SSLSPNELTL VKTPSGTCAV YGSSFKAYTE DCSNPNTYIC MKRAV //