ID   PGD2_HUMAN     STANDARD;      PRT;   198 AA.
AC   O60760;
DT   30-MAY-2000 (Rel. 39, Created)
DT   30-MAY-2000 (Rel. 39, Last sequence update)
DT   10-OCT-2003 (Rel. 42, Last annotation update)
DE   Glutathione-requiring prostaglandin D synthase (EC 5.3.99.2)
DE   (Glutathione-dependent PGD synthetase) (Prostaglandin-H2 D-isomerase)
DE   (Hematopoietic prostaglandin D synthase) (H-PGDS).
GN   PTGDS2 OR PGDS.
OS   Homo sapiens (Human).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC   Mammalia; Eutheria; Primates; Catarrhini; Hominidae; Homo.
OX   NCBI_TaxID=9606;
RN   [1]
RP   SEQUENCE FROM N.A.
RC   TISSUE=Placenta;
RX   MEDLINE=20285358; PubMed=10824118;
RA   Kanaoka Y., Fujimori K., Kikuno R., Sakaguchi Y., Urade Y.,
RA   Hayaishi O.;
RT   "Structure and chromosomal localization of human and mouse genes for
RT   hematopoietic prostaglandin D synthase.";
RL   Eur. J. Biochem. 267:3315-3322(2000).
RN   [2]
RP   SEQUENCE FROM N.A.
RC   TISSUE=Placenta;
RX   MEDLINE=22388257; PubMed=12477932;
RA   Strausberg R.L., Feingold E.A., Grouse L.H., Derge J.G.,
RA   Klausner R.D., Collins F.S., Wagner L., Shenmen C.M., Schuler G.D.,
RA   Altschul S.F., Zeeberg B., Buetow K.H., Schaefer C.F., Bhat N.K.,
RA   Hopkins R.F., Jordan H., Moore T., Max S.I., Wang J., Hsieh F.,
RA   Diatchenko L., Marusina K., Farmer A.A., Rubin G.M., Hong L.,
RA   Stapleton M., Soares M.B., Bonaldo M.F., Casavant T.L., Scheetz T.E.,
RA   Brownstein M.J., Usdin T.B., Toshiyuki S., Carninci P., Prange C.,
RA   Raha S.S., Loquellano N.A., Peters G.J., Abramson R.D., Mullahy S.J.,
RA   Bosak S.A., McEwan P.J., McKernan K.J., Malek J.A., Gunaratne P.H.,
RA   Richards S., Worley K.C., Hale S., Garcia A.M., Gay L.J., Hulyk S.W.,
RA   Villalon D.K., Muzny D.M., Sodergren E.J., Lu X., Gibbs R.A.,
RA   Fahey J., Helton E., Ketteman M., Madan A., Rodrigues S., Sanchez A.,
RA   Whiting M., Madan A., Young A.C., Shevchenko Y., Bouffard G.G.,
RA   Blakesley R.W., Touchman J.W., Green E.D., Dickson M.C.,
RA   Rodriguez A.C., Grimwood J., Schmutz J., Myers R.M.,
RA   Butterfield Y.S.N., Krzywinski M.I., Skalska U., Smailus D.E.,
RA   Schnerch A., Schein J.E., Jones S.J.M., Marra M.A.;
RT   "Generation and initial analysis of more than 15,000 full-length
RT   human and mouse cDNA sequences.";
RL   Proc. Natl. Acad. Sci. U.S.A. 99:16899-16903(2002).
RN   [3]
RP   CHARACTERIZATION.
RC   TISSUE=Megakaryocytes;
RX   MEDLINE=98086344; PubMed=9425264;
RA   Suzuki T., Watanabe K., Kanaoka Y., Sato T., Hayaishi O.;
RT   "Induction of hematopoietic prostaglandin D synthase in human
RT   megakaryocytic cells by phorbol ester.";
RL   Biochem. Biophys. Res. Commun. 241:288-293(1997).
RN   [4]
RP   CHARACTERIZATION.
RC   TISSUE=Megakaryocytes;
RX   MEDLINE=98019190; PubMed=9353279;
RA   Mahmud I., Ueda N., Yamaguchi H., Yamashita R., Yamamoto S.,
RA   Kanaoka Y., Urade Y., Hayaishi O.;
RT   "Prostaglandin D synthase in human megakaryoblastic cells.";
RL   J. Biol. Chem. 272:28263-28266(1997).
CC   -!- FUNCTION: CATALYZES THE CONVERSION OF PGH2 TO PGD2, A
CC       PROSTAGLANDIN INVOLVED IN SMOOTH MUSCLE CONTRACTION/RELAXATION AND
CC       A POTENT INHIBITOR OF PLATELET AGGREGATION.
CC   -!- CATALYTIC ACTIVITY: (5Z,13E)-(15S)-9-alpha,11-alpha-epidioxy-15-
CC       hydroxyprosta-5,13-dienoate = (5Z,13E)-(15S)-9-alpha,15-dihydroxy-
CC       11-oxoprosta-5,13-dienoate.
CC   -!- COFACTOR: Requires glutathione for activity.
CC   -!- SUBCELLULAR LOCATION: Cytoplasmic.
CC   -!- TISSUE SPECIFICITY: EXPRESSED IN A NUMBER OF MEGAKARYOCYTIC CELL
CC       LINES BUT NOT IN PLATELETS. ALSO EXPRESSED IN PLACENTA.
CC   -!- DEVELOPMENTAL STAGE: HIGHEST LEVELS IN IMMATURE MEGAKARYOCYTIC
CC       CELLS. DISAPPEARS AFTER FINAL DIFFERENTIATION TO PLATELETS.
CC   -!- INDUCTION: By 12-O-tetradecanoylphorbol-13-acetate (TPA).
CC   -!- SIMILARITY: Belongs to the GST superfamily. Sigma family.
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DR   EMBL; D82073; BAA25545.1; -.
DR   EMBL; AB008830; BAA96854.1; -.
DR   EMBL; AB008826; BAA96854.1; JOINED.
DR   EMBL; AB008827; BAA96854.1; JOINED.
DR   EMBL; AB008828; BAA96854.1; JOINED.
DR   EMBL; AB008829; BAA96854.1; JOINED.
DR   EMBL; BC020734; AAH20734.1; -.
DR   HSSP; P24472; 1GUK.
DR   MIM; 602598; -.
DR   GO; GO:0005737; C:cytoplasm; TAS.
DR   GO; GO:0004667; F:prostaglandin-D synthase activity; TAS.
DR   GO; GO:0007626; P:locomotory behavior; TAS.
DR   InterPro; IPR004046; GST_Cterm.
DR   InterPro; IPR004045; GST_Nterm.
DR   Pfam; PF00043; GST_C; 1.
DR   Pfam; PF02798; GST_N; 1.
KW   Isomerase; Prostaglandin biosynthesis.
FT   INIT_MET      0      0       BY SIMILARITY.
SQ   SEQUENCE   198 AA;  23212 MW;  1C1B8491B833C56C CRC64;
     PNYKLTYFNM RGRAEIIRYI FAYLDIQYED HRIEQADWPE IKSTLPFGKI PILEVDGLTL
     HQSLAIARYL TKNTDLAGNT EMEQCHVDAI VDTLDDFMSC FPWAEKKQDV KEQMFNELLT
     YNAPHLMQDL DTYLGGREWL IGNSVTWADF YWEICSTTLL VFKPDLLDNH PRLVTLRKKV
     QAIPAVANWI KRRPQTKL
//