ID MCA3_HUMAN Reviewed; 174 AA. AC O43324; Q5THS2; DT 18-OCT-2001, integrated into UniProtKB/Swiss-Prot. DT 01-JUN-1998, sequence version 1. DT 29-APR-2008, entry version 62. DE Eukaryotic translation elongation factor 1 epsilon-1 (Multisynthetase DE complex auxiliary component p18) (Elongation factor p18). GN Name=EEF1E1; Synonyms=P18; OS Homo sapiens (Human). OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; OC Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; OC Catarrhini; Hominidae; Homo. OX NCBI_TaxID=9606; RN [1] RP NUCLEOTIDE SEQUENCE [MRNA]. RC TISSUE=Testis; RA Motegi H., Noda T., Shiba K.; RT "Cloning of cDNA for human p18 from human testis."; RL Submitted (FEB-1998) to the EMBL/GenBank/DDBJ databases. RN [2] RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. RC TISSUE=Umbilical cord blood; RX MEDLINE=98318631; PubMed=9653160; DOI=10.1073/pnas.95.14.8175; RA Mao M., Fu G., Wu J.-S., Zhang Q.-H., Zhou J., Kan L.-X., Huang Q.-H., RA He K.-L., Gu B.-W., Han Z.-G., Shen Y., Gu J., Yu Y.-P., Xu S.-H., RA Wang Y.-X., Chen S.-J., Chen Z.; RT "Identification of genes expressed in human CD34(+) hematopoietic RT stem/progenitor cells by expressed sequence tags and efficient full- RT length cDNA cloning."; RL Proc. Natl. Acad. Sci. U.S.A. 95:8175-8180(1998). RN [3] RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. RA Kalnine N., Chen X., Rolfs A., Halleck A., Hines L., Eisenstein S., RA Koundinya M., Raphael J., Moreira D., Kelley T., LaBaer J., Lin Y., RA Phelan M., Farmer A.; RT "Cloning of human full-length CDSs in BD Creator(TM) system donor RT vector."; RL Submitted (MAY-2003) to the EMBL/GenBank/DDBJ databases. RN [4] RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. RX MEDLINE=22935763; PubMed=14574404; DOI=10.1038/nature02055; RA Mungall A.J., Palmer S.A., Sims S.K., Edwards C.A., Ashurst J.L., RA Wilming L., Jones M.C., Horton R., Hunt S.E., Scott C.E., RA Gilbert J.G.R., Clamp M.E., Bethel G., Milne S., Ainscough R., RA Almeida J.P., Ambrose K.D., Andrews T.D., Ashwell R.I.S., RA Babbage A.K., Bagguley C.L., Bailey J., Banerjee R., Barker D.J., RA Barlow K.F., Bates K., Beare D.M., Beasley H., Beasley O., Bird C.P., RA Blakey S.E., Bray-Allen S., Brook J., Brown A.J., Brown J.Y., RA Burford D.C., Burrill W., Burton J., Carder C., Carter N.P., RA Chapman J.C., Clark S.Y., Clark G., Clee C.M., Clegg S., Cobley V., RA Collier R.E., Collins J.E., Colman L.K., Corby N.R., Coville G.J., RA Culley K.M., Dhami P., Davies J., Dunn M., Earthrowl M.E., RA Ellington A.E., Evans K.A., Faulkner L., Francis M.D., Frankish A., RA Frankland J., French L., Garner P., Garnett J., Ghori M.J., RA Gilby L.M., Gillson C.J., Glithero R.J., Grafham D.V., Grant M., RA Gribble S., Griffiths C., Griffiths M.N.D., Hall R., Halls K.S., RA Hammond S., Harley J.L., Hart E.A., Heath P.D., Heathcott R., RA Holmes S.J., Howden P.J., Howe K.L., Howell G.R., Huckle E., RA Humphray S.J., Humphries M.D., Hunt A.R., Johnson C.M., Joy A.A., RA Kay M., Keenan S.J., Kimberley A.M., King A., Laird G.K., Langford C., RA Lawlor S., Leongamornlert D.A., Leversha M., Lloyd C.R., Lloyd D.M., RA Loveland J.E., Lovell J., Martin S., Mashreghi-Mohammadi M., RA Maslen G.L., Matthews L., McCann O.T., McLaren S.J., McLay K., RA McMurray A., Moore M.J.F., Mullikin J.C., Niblett D., Nickerson T., RA Novik K.L., Oliver K., Overton-Larty E.K., Parker A., Patel R., RA Pearce A.V., Peck A.I., Phillimore B.J.C.T., Phillips S., Plumb R.W., RA Porter K.M., Ramsey Y., Ranby S.A., Rice C.M., Ross M.T., Searle S.M., RA Sehra H.K., Sheridan E., Skuce C.D., Smith S., Smith M., Spraggon L., RA Squares S.L., Steward C.A., Sycamore N., Tamlyn-Hall G., Tester J., RA Theaker A.J., Thomas D.W., Thorpe A., Tracey A., Tromans A., Tubby B., RA Wall M., Wallis J.M., West A.P., White S.S., Whitehead S.L., RA Whittaker H., Wild A., Willey D.J., Wilmer T.E., Wood J.M., Wray P.W., RA Wyatt J.C., Young L., Younger R.M., Bentley D.R., Coulson A., RA Durbin R.M., Hubbard T., Sulston J.E., Dunham I., Rogers J., Beck S.; RT "The DNA sequence and analysis of human chromosome 6."; RL Nature 425:805-811(2003). RN [5] RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. RC TISSUE=Urinary bladder; RX PubMed=15489334; DOI=10.1101/gr.2596504; RG The MGC Project Team; RT "The status, quality, and expansion of the NIH full-length cDNA RT project: the Mammalian Gene Collection (MGC)."; RL Genome Res. 14:2121-2127(2004). RN [6] RP PROTEIN SEQUENCE OF 2-12, CLEAVAGE OF INITIATOR METHIONINE, RP ACETYLATION AT ALA-2, AND MASS SPECTROMETRY. RC TISSUE=Cervix carcinoma; RA Bienvenut W.V., Calvo F., Kolch W.; RL Submitted (MAR-2008) to UniProtKB. RN [7] RP INTERACTION WITH ATM, SUBCELLULAR LOCATION, AND TISSUE SPECIFICITY. RX PubMed=15680327; DOI=10.1016/j.cell.2004.11.054; RA Park B.-J., Kang J.W., Lee S.W., Choi S.-J., Shin Y.K., Ahn Y.H., RA Choi Y.H., Choi D., Lee K.S., Kim S.; RT "The haploinsufficient tumor suppressor p18 upregulates p53 via RT interactions with ATM/ATR."; RL Cell 120:209-221(2005). CC -!- FUNCTION: Positive modulator of ATM response to DNA damage. CC -!- SUBUNIT: Component of the multisynthetase complex which is CC comprised of a bifunctional glutamyl-prolyl-tRNA synthetase, the CC monospecific isoleucyl, leucyl, glutaminyl, methionyl, lysyl, CC arginyl, and aspartyl-tRNA synthetases as well as three auxiliary CC proteins, p18, p48 and p43. Interacts with ATM and ATR. The CC interaction with ATM, which takes place independently of TP53, is CC induced by DNA damage that may occur during genotoxic stress or CC cell growth. The interaction with ATR is enhanced by UV CC irradiation. CC -!- SUBCELLULAR LOCATION: Cytoplasm. Nucleus. Note=Cytoplasmic under CC growth arrest conditions. Translocated into the nucleus when CC growth resumes (S phase) and following DNA damage. CC -!- TISSUE SPECIFICITY: Down-regulated in various cancer tissues. CC -!- INDUCTION: By DNA damaging agents, such as UV, adriamycin, CC actinomycin D and cisplatin. CC -!- SIMILARITY: Contains 1 GST C-terminal domain. CC --------------------------------------------------------------------------- CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms CC Distributed under the Creative Commons Attribution (CC BY 4.0) License CC --------------------------------------------------------------------------- DR EMBL; AB011079; BAA24926.1; -; mRNA. DR EMBL; AF054186; AAC39916.1; -; mRNA. DR EMBL; BT007306; AAP35970.1; -; mRNA. DR EMBL; AL355499; CAH72539.1; -; Genomic_DNA. DR EMBL; AL023694; CAH72539.1; JOINED; Genomic_DNA. DR EMBL; AL451187; CAH72539.1; JOINED; Genomic_DNA. DR EMBL; AL023694; CAI21644.1; -; Genomic_DNA. DR EMBL; AL355499; CAI21644.1; JOINED; Genomic_DNA. DR EMBL; AL451187; CAI21644.1; JOINED; Genomic_DNA. DR EMBL; AL451187; CAI14747.1; -; Genomic_DNA. DR EMBL; AL023694; CAI14747.1; JOINED; Genomic_DNA. DR EMBL; AL355499; CAI14747.1; JOINED; Genomic_DNA. DR EMBL; BC005291; AAH05291.1; -; mRNA. DR RefSeq; NP_004271.1; -. DR UniGene; Hs.631818; -. DR PhosphoSite; O43324; -. DR PeptideAtlas; O43324; -. DR Ensembl; ENSG00000124802; Homo sapiens. DR GeneID; 9521; -. DR KEGG; hsa:9521; -. DR H-InvDB; HIX0005569; -. DR HGNC; HGNC:3212; EEF1E1. DR MIM; 609206; gene. DR PharmGKB; PA27648; -. DR LinkHub; O43324; -. DR ArrayExpress; O43324; -. DR CleanEx; HS_EEF1E1; -. DR GermOnline; ENSG00000124802; Homo sapiens. DR GO; GO:0005737; C:cytoplasm; ISS:UniProtKB. DR GO; GO:0005634; C:nucleus; ISS:UniProtKB. DR GO; GO:0005515; F:protein binding; ISS:UniProtKB. DR GO; GO:0008285; P:negative regulation of cell proliferation; ISS:UniProtKB. DR GO; GO:0043065; P:positive regulation of apoptosis; ISS:UniProtKB. DR GO; GO:0043517; P:positive regulation of DNA damage response,...; ISS:UniProtKB. DR InterPro; IPR010987; Glutathione-S-Trfase_C-like. DR InterPro; IPR004046; GST_C. DR Gene3D; G3DSA:1.20.1050.10; GST_C_like; 1. DR Pfam; PF00043; GST_C; 1. DR PROSITE; PS50405; GST_CTER; 1. PE 1: Evidence at protein level; KW Acetylation; Cytoplasm; Direct protein sequencing; Nucleus; KW Protein biosynthesis. FT INIT_MET 1 1 Removed. FT CHAIN 2 174 Eukaryotic translation elongation factor FT 1 epsilon-1. FT /FTId=PRO_0000221132. FT DOMAIN 50 173 GST C-terminal. FT MOD_RES 2 2 N-acetylalanine. SQ SEQUENCE 174 AA; 19811 MW; 58AAE4BD9E1684E2 CRC64; MAAAAELSLL EKSLGLSKGN KYSAQGERQI PVLQTNNGPS LTGLTTIAAH LVKQANKEYL LGSTAEEKAI VQQWLEYRVT QVDGHSSKND IHTLLKDLNS YLEDKVYLTG YNFTLADILL YYGLHRFIVD LTVQEKEKYL NVSRWFCHIQ HYPGIRQHLS SVVFIKNRLY TNSH //